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An endogenous control gene shows expression levels that are relatively constant and moderately abundant across tissues, cell types, and treatment protocols. Such genes are also known as normalizer genes, housekeeping genes, and reference genes. Normalization to endogenous control genes is currently the most accurate method to correct for potential biases that are caused by:

  • Sample collection
  • Variation in the amount of starting material
  • Reverse transcription (RT) efficiency
  • Nucleic acid (RNA/DNA) preparation and quality

No single gene can act as a universal endogenous control for all experimental conditions, so we recommend verifying the chosen endogenous control or set of controls for the sample tissue, cell, or treatment. TaqMan Endogenous Control Assays are a collection of assays targeting genes that are commonly used in quantitative PCR gene expression experiments. Each assay consists of two primers and a TaqMan probe that specifically amplifies and detects sequences derived from specific gene transcripts. TaqMan Endogenous Control Assays are offered in multiple formats and scales for ease of use and economy.

Our convenient collection of pre-designed TaqMan probe and primer sets enables you to normalize your gene expression data based on RNA sample amount. Endogenous control assays are available for the most commonly used human, mouse, and rat genes, as well as 18S eukaryotic ribosomal RNA.

Individual TaqMan Endogenous Control Assays

Certain genes such as ACTB, GAPDH, and 18S are commonly used as endogenous controls.  Choosing from these popular genes increases your chances of finding a suitable endogenous control.  Testing at least 2-3 candidate endogenous controls is recommended.  Ordering individual TaqMan Endogenous Control assays is a cost-effective solution for testing a small number of candidates across many samples.  Click below to see a list of popular endogenous control candidates for a variety of species.

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TaqMan Endogenous Control array plates and cards

If there is uncertainty around which endogenous control is most accurate for a specific experiment, endogenous control candidates may be tested in an easy, economical way using TaqMan arrays. We offer endogenous control panels in standard 96-well array plates, Fast 96-well array plates, and TaqMan array cards. These arrays may be purchased individually, maximizing flexibility to test as few or as many samples as needed. Once the candidate gene with the lowest variation has been identified from the array data, the individual TaqMan Endogenous Control Assay for that gene may be purchased for the final experiment.

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Literature

For Research Use Only. Not for use in diagnostic procedures