Biosystems QuantStudio 3 Real-Time PCR 

Intuitive and easy to use for all levels of experience

Now you can get up and running quickly with the Applied Biosystems QuantStudio 3 Real-Time PCR System, a simple and affordable real-time PCR solution designed for users with all levels of experience. Get connected to your data easier than before with a real-time PCR systems that offers an interactive touch screen and the option to choose Web browser-based software enabled through the Thermo Fisher Cloud.

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The QuantStudio 3 Real-Time PCR System is available with 96-well thermal blocks with either 0.1 mL or 0.2 mL well volume.

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Contact us
Telephone: 800 327 3002, option 4, then option 3 (toll-free in the US only)
Telephone: 00 800 5345 5345, option 3 (toll-free in Europe, Middle East and South Africa only)

Get started quickly—The factory-calibrated system is delivered in a user-friendly package, enabling quick installation and immediate use.

Skip the learning curve—With preoptimized templates and an intuitive interface that requires minimal training, the QuantStudio Design and Analysis Software is available in desktop and Web browser-based format.

Helps save time with efficient experiment runs—Applied Biosystems™ VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as 30 minutes.

Results you can trust—Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.

Get started with a limited budget—Get the modern industrial design and superior functionality you have come to know with the Applied Biosystems™ QuantStudio family of high-performance instruments. The QuantStudio 3 instrument is the perfect entry-level system and allows you to stretch your budget.

Get connected to your data more easily and quickly than ever before, with a real-time PCR system that offers an interactive touch screen for a modern usability experience similar to mobile devices and the option to choose Web browser–based software enabled through the Thermo Fisher Cloud. With the cloud, you can:

  • Remotely monitor your runs and access data anytime, anywhere*
  • Analyze sophisticated data sets quickly
  • Store results in a secure data storage space
  • Share and collaborate with colleagues at your institution or around the world

For peace of mind, the Thermo Fisher Cloud offers high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services® (AWS) to offer a robust platform.

Learn more

*Where the Web is accessible

Utilizing proven Applied Biosystems OptiFlex technology and VeriFlex blocks, the QuantStudio 3 system offers improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.

Precise quantification with 1.5-fold discrimination

Figure 5-A

A

Figure 5-B

B

Figure 1. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with Applied Biosystems PE2 TaqMan. Assay under Fast run conditions using TaqMan. Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.

Excellent reproducibility and 10-log dynamic range

Figure 6

Figure 2. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.


The QuantStudio 3 system also supports probe-based assays and intercalating dyes. TaqMan probe-based assays enable outstanding specificity and sensitivity. Applied Biosystems SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 3 system has four filters calibrated for FAM/SYBR Green, VIC, ABY/NED/TAMRA, and JUN/ROX dyes. 

Melt curve analysis

Figure 3

Figure 3. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems SYBR Select Master Mix with primers for RNase P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).

Genotyping analysis

Figure 4

Figure 4. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using Applied Biosystems TaqMan. SNP Genotyping Assay C_29086771_20, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of real-time PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.

  Repair Center service plans On-site service plans
Service plans at a glance AB Repair Center Support Plus AB Maintenance Plus AB Assurance AB Complete
On-site response time   Target 2 business days* 2 business days* Next business day*
Scheduled on-site planned maintenance (PM)   Yes Yes Yes
Remote diagnostics Yes Yes Yes Yes
Parts, labor, and travel for repair included Yes 10% discount Yes Yes
Computer repair and replacement included Yes 10% discount Yes Yes
Priority access to Tech Support
Mon–Fri, 8 a.m.—5 p.m. local time
Yes   Yes Yes
Priority access to Remote Service Engineer Yes   Yes Yes
Requalification post-PM and critical repairs       Yes
Field Application Scientist consultation       Yes
Loaner instrument issued during repair (Repair Center plans only) Yes      

*Availability limited in some geographic areas.

Get started quickly—The factory-calibrated system is delivered in a user-friendly package, enabling quick installation and immediate use.

Skip the learning curve—With preoptimized templates and an intuitive interface that requires minimal training, the QuantStudio Design and Analysis Software is available in desktop and Web browser-based format.

Helps save time with efficient experiment runs—Applied Biosystems™ VeriFlex™ technology offers 3 independent temperature zones for PCR optimization, and fast thermal cycling enables you to get results in as little as 30 minutes.

Results you can trust—Applied Biosystems™ OptiFlex™ technology with white LED and 4 coupled channels enables optimal well-to-well and instrument-to-instrument accuracy.

Get started with a limited budget—Get the modern industrial design and superior functionality you have come to know with the Applied Biosystems™ QuantStudio family of high-performance instruments. The QuantStudio 3 instrument is the perfect entry-level system and allows you to stretch your budget.

Get connected to your data more easily and quickly than ever before, with a real-time PCR system that offers an interactive touch screen for a modern usability experience similar to mobile devices and the option to choose Web browser–based software enabled through the Thermo Fisher Cloud. With the cloud, you can:

  • Remotely monitor your runs and access data anytime, anywhere*
  • Analyze sophisticated data sets quickly
  • Store results in a secure data storage space
  • Share and collaborate with colleagues at your institution or around the world

For peace of mind, the Thermo Fisher Cloud offers high industry-standard security, so your work remains secure. We have partnered with Amazon Web Services® (AWS) to offer a robust platform.

Learn more

*Where the Web is accessible

Utilizing proven Applied Biosystems OptiFlex technology and VeriFlex blocks, the QuantStudio 3 system offers improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.

Precise quantification with 1.5-fold discrimination

Figure 5-A

A

Figure 5-B

B

Figure 1. The QuantStudio 3 and 5 systems provide sensitive detection and high-confidence target discrimination down to 1.5-fold differences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with Applied Biosystems PE2 TaqMan. Assay under Fast run conditions using TaqMan. Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.

Excellent reproducibility and 10-log dynamic range

Figure 6

Figure 2. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.


The QuantStudio 3 system also supports probe-based assays and intercalating dyes. TaqMan probe-based assays enable outstanding specificity and sensitivity. Applied Biosystems SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 3 system has four filters calibrated for FAM/SYBR Green, VIC, ABY/NED/TAMRA, and JUN/ROX dyes. 

Melt curve analysis

Figure 3

Figure 3. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using Applied Biosystems SYBR Select Master Mix with primers for RNase P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).

Genotyping analysis

Figure 4

Figure 4. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using Applied Biosystems TaqMan. SNP Genotyping Assay C_29086771_20, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of real-time PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.

  Repair Center service plans On-site service plans
Service plans at a glance AB Repair Center Support Plus AB Maintenance Plus AB Assurance AB Complete
On-site response time   Target 2 business days* 2 business days* Next business day*
Scheduled on-site planned maintenance (PM)   Yes Yes Yes
Remote diagnostics Yes Yes Yes Yes
Parts, labor, and travel for repair included Yes 10% discount Yes Yes
Computer repair and replacement included Yes 10% discount Yes Yes
Priority access to Tech Support
Mon–Fri, 8 a.m.—5 p.m. local time
Yes   Yes Yes
Priority access to Remote Service Engineer Yes   Yes Yes
Requalification post-PM and critical repairs       Yes
Field Application Scientist consultation       Yes
Loaner instrument issued during repair (Repair Center plans only) Yes      

*Availability limited in some geographic areas.