RNA sequencing (RNA-seq) using next-generation sequencing (NGS) has proven to be a powerful technique for novel transcript discovery, fusion-transcript detection, splicing analysis, genome-binding protein mapping, and gene expression profiling. The Ion S5™ and Ion S5™ XL Systems enable a broad spectrum of RNA-seq applications in a scalable and simple NGS workflow. To get started, choose an RNA-seq application from the following table and download the appropriate user guide for the step you are interested in.

Sequencing workflow for RNA analysis

Documents and resources


User guides and FAQs


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Training, events, and webinars