Validating cell line quality is critical to stem cell research. Therefore, we offer you maximum flexibility in antibody and dye choices to enable you to specifically detect your pluripotent stem cells (PSCs) and their derivatives.
Antibodies for Pluripotent Stem Cell Detection
Superior imaging for PSCs in one box
Everything you need for superior image-based analysis of hPSCs and a variety of lineages is now available in one box. Introducing Molecular Probes Immunocytochemistry and live imaging kits for stem cells. Kits offer a combination of antibodies, stains, buffers, and/or media to create beautiful, high-quality images of stem cells.
Stand-alone Pluripotent Stem Cell Antibodies
|Target Antigen||Reactivity||Host||Clonality||Validation||Cat. No.|
|E-Cadherin||Human||Mouse||Monoclonal||WB, IHC, IP, IF, FC, ELISA||13-1700|
|TRA 1-60||Human||Mouse||Monoclonal||WB, IHC, IF, FC, ELISA||41-1000|
|TRA 1-81||Human||Mouse||Monoclonal||WB, IHC, IF, FC, ELISA||41-1100|
|SSEA-3/4||Human, Mouse||Mouse||Monoclonal||IHC, IF, FC||41-4000|
WB = Western Blot; IHC = Immunohistochemistry; IP = Immunoprecipitation; IF = Immunofluorescence; FC = Flow Cytometry
These primary antibodies can be conjugated directly with Alexa Fluor dyes or combined with our vast suite of secondary conjugated antibodies. Stained cells can be visualized by the FLoid Cell Imaging Station (Figures 4-6).
Figure 4. H9 hESCs were grown on feeders and stained (without fixing) with SSEA-4 primary antibody and an Alexa Fluor 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 100x magnification using a FITC filter.
Figure 5. H9 hESCs were grown on feeders and stained (without fixing) with TRA-1-81 primary antibody and an Alexa Fluor 488–conjugated anti–mouse IgG secondary antibody. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast and a FITC filter.
Figure 6. H9 hESCs were grown on feeders, fixed with PFA, stained with TRA-1-60 primary antibody and an Alexa Fluor 488–conjugated anti–mouse IgG secondary antibody, and counterstained with DAPI nuclear stain. The cells were visualized at 50x magnification. Shown is a merged image using phase contrast, and FITC and Hoescht filters.