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JunD Antibody (720035) in ChIP
Chromatin Immunoprecipitation (ChIP) was performed using Anti-JunD Rabbit Polyclonal Antibody (Product # 720035, 5 ug) on sheared chromatin from 2 million HeLa cells (serum starved for 24 hours and serum released for 2 hours) using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system (Product # 4351106) with optimized PCR primer pairs for the promoters of the active VEGF, CREB5 genes, used as positive control target gene, and the promoter region of the GAPDH, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
