c-Myc Epitope Tag Antibody (R95325) in Flow

Flow cytometry analysis of c-Myc Epitope Tag, FITC conjugate was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with c-Myc Epitope Tag, FITC conjugate Mouse Monoclonal Antibody (R95325, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA and incubated at room temperature for 2 hours. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

Flow cytometry analysis of c-Myc Epitope Tag, FITC conjugate was done on SH-SY5Y cells. Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature. Cells were labeled with c-Myc Epitope Tag, FITC conjugate Mouse Monoclonal Antibody (R95325, red histogram) or with mouse isotype control (yellow histogram) at 3-5 ug/million cells in 2.5% BSA and incubated at room temperature for 2 hours. The representative 10,000 cells were acquired and analyzed for each sample using an Attune® Acoustic Focusing Cytometer. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

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