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H9 human embryonic stem cells (ESCs) were cultured on mouse embryonic fibroblasts (MEFs) with 20% ESC-Qualified Fetal Bovine Serum (FBS) or 20% KnockOut SR – Multi-Species. 129S6 mouse ESCs were cultured on MEFs using 15% ESC-Qualified FBS or 15% KnockOut SR – Multi-Species. Human ESC colonies were more compact in KSR – Multi-Species compared to FBS. Mouse ESCs in KSR – Mutli-Species were dome-shaped and more compact than in FBS.
