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Effective removal of genomic DNA using dsDNase
Two-step RT-qPCR analysis of PBGD gene with (RT+) and without (RT-) reverse transcriptase. cDNA synthesis was performed from 0.2ng total Jurkat RNA using Maxima First Strand cDNA Synthesis Kit with dsDNase (#K1671) or other kits including gDNA removal step. Flat RT-plot with Maxima kit indicates complete removal of contaminating gDNA, whereas RT-reactions with other suppliers' kits indicate amplification of residual gDNA.
