Lipofectamine RNAiMAX Transfection Reagent

Get superior transfection for your gene knockdown studies

Invitrogen Lipofectamine RNAiMAX Transfection Reagent offers an advanced, efficient solution for siRNA delivery. No other siRNA specific transfection reagent provides such easy and efficient siRNA delivery in a wide variety of cell lines including common cell types, stem cells and primary cells, as well as traditionally hard-to-transfect cell types.

  • Unmatched performance, delivering greater knockdown with less siRNA
  • Easy optimization with a simple protocol helps ensure great performance

Video protocol: siRNA transfection

View the video to see the steps needed to perform siRNA transfection with Lipofectamine RNAiMAX reagent.

Superior transfection at low siRNA concentrations

When it comes to achieving effective gene knockdown, Lipofectamine RNAiMAX Transfection Reagent easily outperforms other siRNA transfection reagents. High knockdown levels of target genes can be achieved with as little as 1 nM siRNA (Figure 1). The Lipofectamine RNAiMAX protocol recommends using 10 nM siRNA as a starting point to maximize knockdown levels.

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Figure 1. Superior knockdown with Lipofectamine RNAiMAX Transfection Reagent compared to competing siRNA transfection reagents. At both 10 nM and 1 nM p53 siRNA, Lipofectamine RNAiMAX Transfection Reagent provides more effective knockdown relative to nontransfected cells than other RNAi reagents, including siLentFect™ (Bio-Rad), DharmaFECT™ (Dharmacon), and HiPerFect (Qiagen) reagents.

 

Low cytotoxicity profile for easy optimization

Lipofectamine RNAiMAX Transfection Reagent gives maximal knockdown and excellent cell viability across a 10-fold concentration range of the reagent (Figure 2). This makes Lipofectamine RNAiMAX reagent easy to optimize for the lowest siRNA concentration while reducing cytotoxicity in your experimental system. Transfection-mediated cytotoxicity can mask the true phenotype of a target gene being studied, so minimizing the amount of reagent used in your transfections is a critical factor for successful RNAi experiments.

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Figure 2. Optimal knockdown and minimal cytotoxicity in A549 cells transfected with Lipofectamine RNAiMAX Transfection Reagent. A range of 0.1 μL to 1.0 μL of Lipofectamine RNAiMAX reagent was used, resulting in efficient knockdown of the p53 gene expression level while maintaining excellent cell viability.

 

Simple protocol for easy optimization in a broad spectrum of cell lines

With its ability to transfect a broad range of cell types, Lipofectamine RNAiMAX reagent, combined with the Invitrogen BLOCK-iT Alexa Fluor Red Fluorescent Control, gives you the most versatile approach to all of your knockdown experiments (Table 1, Figure 3). Lipofectamine RNAiMAX offers a simple protocol—no need to remove transfection complexes, or change or add medium following transfection. You’ll spend less hands-on time and get results faster.

Table 1. Achieve transfection perfection on a wide range of cell types with Lipofectamine RNAiMAX Transfection Reagent.

Cell line/type Cell type
MDA-MB-435 Breast cancer
HeLa Cervical carcinoma
HT1080 Human fibrosarcoma
A549 Lung carcinoma
HepG2 Liver carcinoma
MCF7 Breast cancer
SK-N-SH Neuroblastoma
Mesenchymal stem cells Bone marrow
HAMSC Human aortic smooth muscle cells
HEKa Human epidermal keratinocytes
HRE Human renal epithelial cells
NTERA-2 Embryonal carcinoma cells
HUVEC Human umbilical vein endothelial cells
ADSC Adipose-derived stem cells
HCT116 Colon carcinoma
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Figure 3. Improve your transfection efficiencies using the BLOCK-iT Alexa Fluor Red Fluorescent Control. HeLa cells were transfected with the BLOCK-iT Alexa Fluor Red Fluorescent Control (50 nM) using Lipofectamine RNAiMAX Transfection Reagent. A. Twenty-four hours after transfection, nearly 100% of the cells take up the control and retain a normal morphology as seen in the bright-field image. B.

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