High-Fidelity DNA Polymerases & Master Mixes—Thermo Scientific
High-fidelity PCR enzymes are used for applications requiring high accuracy during DNA amplification such as cloning, sequencing or mutagenesis. Thermo Scientific Phusion High-Fidelity DNA Polymerase was created by fusing a dsDNA-binding domain to a Pyrococcus-like proofreading polymerase. Due to this unique fusion technique, Phusion DNA Polymerases have extremely low error rates and are considered a gold standard for high-fidelity PCR.
Thermo Scientific high-fidelity DNA polymerase selection table
Phusion High-Fidelity DNA Polymerase | Phusion Hot Start II High-Fidelity DNA Polymerase | Phusion Flash High-Fidelity PCR Master Mix | Phusion U Hot Start DNA Polymerase | |
---|---|---|---|---|
Fidelity vs. Taq | 52x | 52x | 25x | 25x |
Amplicon size | Up to 20 kb | Up to 20 kb | Up to 20 kb | Up to 20 kb |
Enhanced specificity | ✓ | ✓ | ✓ | |
Fast cycling | ✓ | |||
dUTP incorporation | ✓ | |||
Stand-alone enzyme | ||||
Master mix | ||||
*Contain green buffer that includes density reagent and two tracking dyes for direct loading of PCR products on gels
Additional high-fidelity PCR enzymes:
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