Mesophilic and thermophilic DNA polymerases for different polymerization reactions, DNA end-blunting and amplification, labeling and others.
Properties of DNA polymerases
Polymerase |
Applications |
Template |
3' - 5' exo- nuclease |
5' - 3' exo- nuclease |
Strand displace- ment |
DNA Polymerase I |
- DNA labeling by nick-translation
- Second strand cDNA synthesis
|
DNA |
 |
 |
|
Klenow Fragment |
- DNA blunting by fill-in 5'- overhangs
- Random-primed DNA labeling
- Labeling by fill-in 5'- overhangs of dsDNA
- DNA sequencing
- Site-specific mutagenesis
- Second strand cDNA synthesis
|
DNA |
 |
|
 |
Klenow Fragment, exo- |
- Random-primed DNA labeling
- Labeling by fill-in 5'- overhangs of dsDNA
- Strand displacement amplification
- DNA sequencing
|
DNA |
|
|
 |
T4 DNA Polymerase |
- Blunting of DNA with 5'- or 3'- overhangs
- Blunting of PCR products with 3'-dA overhangs
- DNA labeling
- Site-directed mutagenesis
- Ligation-independent cloning
|
DNA |
 |
|
|
T7 DNA Polymerase |
- Purification of covalently closed circular DNA
- Primer extension reactions on long templates
- DNA 3'-end labeling
- Strand extensions in site directed mutagenesis
- Fill-in blunting of 5'-overhang DNA
- Second strand cDNA synthesis
- In situ detection of DNA fragmentation associated with apoptosis
|
DNA |
 |
|
|
Terminal deoxynucleotidyl transferase (TdT) |
- Production of synthetic homo-, heteropolymers
- Homo polymeric tailing of linear duplex DNA with 3'-OH terminus
- Oligodeoxyribonucleotide and DNA labeling
- 5'-RACE
|
ssDNA dsDNA ssRNA dsRNA |
|
|
NA |
Terminal deoxynucleotidyl transferase (TdT), Recombinant |
- Production of synthetic homo-, heteropolymers
- Homo polymeric tailing of linear duplex DNA with 3'-OH terminus
- Oligodeoxyribonucleotide and DNA labeling
- PCR, RNA analysis, genomic DNA cloning, 5' RACE, sequencing, and labeling of DNA probes
|
ssDNA dsDNA ssRNA dsRNA |
|
|
NA |
phi29 DNA Polymerase |
- Rolling circle amplification
- Multiple displacement amplification
- Whole genome amplification(WGA)
- DNA sequencing
- Protein-primed DNA amplification
- RNA-primed DNA amplification
|
DNA |
 |
|
 |
Bsm DNA Polymerase |
- Loop-mediated isothermal amplification (LAMP)
- Whole genome amplification (WGA)
- Ramification amplification (RAM)
- Random-primed DNA labeling
- Labeling by fill-in 5'-overhangs of dsDNA
|
DNA |
|
|
 |
EP0041,EP0042,EP0051,EP0052,EP0054,EP0061,EP0062,EP0081,EP0091,EP0092,EP0094,EP0161,EP0162,72033.2500.UN,EP0421,EP0422,EP0691