Pacific Orange dye is a bright orange-fluorescent dye optimally excited by the 405 nm line of the violet laser and with excitation/emission at 400/551 nm. Conjugates of this dye are strongly fluorescent even at neutral pH.
Pacific Blue, Pacific Green and Pacific Orange dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm, 500 nm and 551 nm, respectively, facilitating three-color analysis.
We offer Pacific Orange dye conjugated to a variety of primary antibodies, secondary antibodies and streptavidin. In addition, reactive and protein labeling kits are available so that you can produce your own antibody conjugates or probes.
 Initial brightness |
Pacific Orange dye is an orange fluorophore used primarily for flow cytometry, optimally excited by the 405 nm violet laser line. | ||
 Photostability in buffer |
| 6 | 405 | TRITC | 400 | 551 | ||
 Stain index |
 Laser line |
 Common filter set |
 Excitation max |
 Emission max |
We offer a broad range of Pacific Orange dye products including labeling kits, labeled antibodies, bioconjugates and more:
Fluorescence excitation and emission spectra of Pacific Orange dye.
Select dye-labeling chemistries for conjugation with the following reactive groups:
| Amine |
|---|
Select an optimized labeling kit for your target antibody or protein:
| Target protein | Kit | Cat. No. |
|---|---|---|
| Site-specific antibody labeling | ||
| <1–20 µg mouse IgG1 | Zenon Pacific Orange Mouse IgG1 Labeling Kit | Z25256 |
| Conventional antibody/protein labeling | ||
| 100 µg antibody/protein | Pacific Orange Antibody/Protein Labeling Kit | P30014 |
| 1 mg antibody/protein | Pacific Orange Antibody/Protein Labeling Kit | P30016 |
We offer Pacific Blue, Pacific Green and Pacific Orange dyes as well as Qdot probes for use with the violet laser. In addition to primary and secondary antibody conjugates of these dyes, we’ve developed kits for cell proliferation, immunophenotyping, dead cell discrimination, apoptosis, and cell cycle analysis—all aimed at helping you maximize the data you can get from your violet laser–equipped flow cytometer.
Learn more about Cell Health and Viability Assays for Flow Cytometry
Excitation spectra (dotted lines) and emission spectra (solid lines) for Molecular Probes violet laser–excited Pacific Blue dye (blue), Pacific Green dye (green), and Pacific Orange dye (orange). The shaded area represents the emission bandpass filter (522/31) used for detection of the Pacific Green dye.
The significantly revised 11th Edition is the most complete fluorescence labeling and detection reference available. The Molecular Probes Handbook can help you achieve the best experimental results for your fluorescence labeling and detection experiments. View the handbook online or order your own copy.
Keep your flow cytometry running at peak efficiency with instrument set-up, calibration, and counting beads including alignment and linearity beads, compensation tools, and cell-counting beads.
Stain your own cell by using our simulation tool to develop reproducible results with many of our signature fluorescent dyes. Develop your multiplexing strategy for antibody probes, reactive dyes, and counterstains to create your perfectly labeled fluorescent cell.
Plot and compare spectra and check the spectral compatibility for common fluorophores including all of the fluorescent probes from Thermo Fisher Scientific. The Fluorescence SpectraViewer tool is formatted for both microscope imaging and flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures.
