Which RNA Isolation Kit is Right for Your qRT-PCR Experiment?

  Gold standard for highly pure, intact RNA   High-quality RNA in less than 20 minutes   High-throughput purification of RNA and DNA   Complete, no purification system for qRT-PCR results
 
 
 
 
 
 
 
 
Recommendations Most Cited, Top Seller           Best for qRT-PCR
Isolation method Organic reagent   Silica filter, column format (convenience)   Magnetic beads (scalability, flexibility)   Chemical lysis (time to qRT-PCR results)
High throughput compatible No   Yes (plate format available)   Yes   Yes
RT reagents included No   No   No   Yes
qPCR reagents included No   No   No   Yes
Prep time ~1hr   < 20 minutes   ~45 minutes   10 minutes
Compatible sample types Most sample types, particularly more difficult to lyse   Cells and tissues   Cells, blood, plants   Cells
Amount of starting material 100 mg of tissue or 107 cells (requires 1ml reagent)   Up to 200 mg tissue, 5x106 to 1 x 108 cells   Up to 100 mg tissue, Up to 5x106 cells   1–10,000 cells
  Order Now   Order Now   Order Now   Order Now

Eliminate Genomic DNA Contamination

Genomic DNA contamination can lead to false positive RT-PCR results. We offer a variety of Ambion® tools for minimizing genomic DNA contamination from RNA samples prior to RT-PCR. For example, DNA-free™ DNase Treatment and Removal Reagents are designed for removing contaminating DNA from RNA samples and for the removal of DNase after treatment without Proteinase K treatment and organic extraction. In addition, we offer TURBO DNase™ enzyme kits, a hyperactive enzyme engineered from wild-type bovine DNase. The proficiency of TURBO DNase™ enzyme in binding very low concentrations of DNA means that the enzyme is particularly effective in removing trace quantities of DNA contamination.  To prevent cross contamination during PCR experiments, we also offer DNAZap™ DNA Degradation Solution and RNase-free barrier pipette tips.

Less time to real-time for your RNA applications.