Purifying RNA from cultured cells has traditionally been a highly repetitive, time- and labor-intensive procedure, often involving the use of toxic organic chemicals. The products developed by our RNA experts allow you to isolate RNA of maximum yield, purity, and integrity using several technology formats (organic extraction, glass-fiber columns, magnetic beads, and combinations of these).

You can also analyze cultured cell lysates without first isolating the RNA. In significantly less time, you’ll get qPCR results equivalent to - or in some cases better than - those from purified RNA. The Cells-to-CT™ and Single Cell-to-CT™ product lines offer tremendous performance and handling advantages.

Which RNA Isolation Kit from Cultured Cells is Right for You?

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  Highly pure, intact RNA Choice for long template with enhanced fidelity & specificity Complete system—cells to qRT-PCR with TaqMan® Master Mix Complete system—cells to qRT-PCR from 1-10 cells
  TRIzol® Reagent Power SYBR® Green Cells-to-CT™ Kit TaqMan® Gene Expression Cells-to-CT™ Kit Ambion® Single Cell-to-CT™ Kit
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Prep time 1 hr <10 minutes <10 minutes <10 minutes
Cell lysis method High purity organic extraction (requires alcohol precipitation) Cells-to-CT™ lysis Cells-to-CT™ lysis Cells-to-CT™ lysis
Pre-amplification reagents included No No No TaqMan® PreAmp Master Mix
Reverse transcription reagents included No MuLV MuLV Superscript VILO™
PCR Enzyme included No AmpliTaq Gold® AmpliTaq Gold® Platinum® Taq Hi-Fi
Master mix included No SYBR® Green TaqMan® TaqMan®
Amount of Starting material Up to 1 x 107 cells 10–100,000 cells 10–100,000 cells 1–10 cells
Application All RNA expression methods qRT-PCR qRT-PCR qRT-PCR

Preserve and Stabilize Your RNA

RNAlater® Solution is widely used for the preservation of RNA in intact animal tissues, organs, and cells, and bacteria. It eliminates the need to immediately process samples for RNA isolation or to freeze them in liquid nitrogen for later processing.