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Celebrating over 20 years of innovation in real-time PCR

As a leader in PCR innovation, we offer you the gold standard in real-time PCR instrumentation. With our Applied Biosystems real-time PCR platforms, you get true value with excellent performance, reliability, and world-class support. Designed for compatibility with Applied Biosystems TaqMan Assays and the flexibility of unlimited real-time chemistry choices, we make it easy for you to get started with your experiments. The Applied Biosystems QuantStudio family of instruments enables you to obtain the results you need, connect and collaborate with colleagues, and achieve your research goals.

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Compare real-time PCR systems

  Simply accessible for new users Simple to use for all levels of experience Easy controls for optimized experiments
 
 

 

 

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Reactions per Run 96 96 96 or 384
Block Format 96-Well-0.2 mL (isothermal) 96-Well 0.2-mL or 96-Well Fast 0.1-mL 96-Well 0.2-mL, 96-Well Fast 0.1-mL, or 384-Well
Color Combinations (Multiplexing) Up to 3 Up to 4 96-well up to 21
384-well up to 5
Run Time Approximately 40 min As fast as 30 min As fast as 30 min
Programmable Volumes 1-200 μL
Validated volumes
10-100 μL
10-100 µl (96-well 0.2mL)

10-30 µl (96-well 0.1mL)
10-100 µl (96-well 0.2mL)

10-30 µl (96-well 0.1mL)

5-20 µl (384-well)
Automation Compatible No No No
Remote Monitoring
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On-Board Memory Yes, >2,000 runs
Email Notification
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Assist with 21 CFR Part 11 Compliance     Security, auditing, e-signature package included
Sensitivity Down to 1 copy
Dynamic Range Up to 10 logs**
Chemistry All real-time PCR-based chemistries.  Flexibility for chemistries with or without passive reference dye.
Applications Available:
 
GeneExpression
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Genotyping
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Copy Number Variation
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Protein Detection
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MicroRNA
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Pathogen Detection
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HRM      
IVD Platform No No Available in certain countries
Temperature Uniformity ± 0.4°C
Excitation Source OptiFlex™ System with white LED light
Filters/Colors 3 excitation filters (450-600 nm)
3 emission filters (500-640 nm)
4 excitation filters (450–600 nm)
4 emission filters (500–640 nm)
96-well: 6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)

384-well: 5 excitation filters (450–650 nm)
5 emission filters (500–700 nm)
Thermo Fisher Cloud Direct connection
Wi-Fi Connectivity Yes
Size W 27 cm
D 50 cm
H 40 cm
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  High flexibility and intuitive design High versatility and intuitive design Maximum throughput, flexibility, and scalability
 
 

 

 

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Reactions per Run 96 or 384 96 or 384 96, 384, or 12,228
Block Format 96, 96 Fast, 384, upgradeable to TaqMan Array (interchangeable) 96, 96 Fast, 384, TaqMan Array (inter- changeable) 96, 96 Fast, 384, TaqMan Array, Applied Biosystems OpenArray
(interchangeable)
Color Combinations (Multiplexing) Up to 5 Up to 21 Up to 21*
Run Time As fast as 30 min
Programmable Volumes 1–200 μL
Validated volumes 5–100 μL 1–100 μL 33 nL–100 μL
Automation Compatible Upgradable to automation capability Yes Yes
Remote Monitoring Yes Yes Yes
On-Board Memory Yes, >100 runs
Email Notification Yes Yes Yes
Assist with 21 CFR Part 11 Compliance Security, auditing, e-signature package available separately
Sensitivity Down to 1 copy
Dynamic Range Up to 10 logs**
Chemistry All real-time PCR-based chemistries. Flexibility for chemistries with or without passive reference dye.
Applications Available:  
GeneExpression
  •  
  •  
  •  
Genotyping
  •  
  •  
  •  
Copy Number Variation
  •  
  •  
  •  
Protein Detection
  •  
  •  
  •  
MicroRNA
  •  
  •  
  •  
Pathogen Detection
  •  
  •  
  •  
HRM
  •  
  •  
  •  
IVD Platform No Available in certain countries No
Temperature Uniformity ±0.5°C
Excitation Source OptiFlex System with halogen lamp
Filters/Colors 5 excitation filters (450–650 nm)
5 emission filters (500–700 nm)
6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)
6 excitation filters (450–670 nm)
6 emission filters (500–720 nm)
Thermo Fisher Cloud Connect to cloud through computer
Wi-Fi Connectivity No
Size W 50.5 cm
D 67.2 cm
H 73.8 cm
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*Duplex capabilities with OpenArray and multiplex capabilities for other formats.
**Up to 7 logs with OpenArray.
  Absolutely attainable chip-based digital PCR
 
 

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Reactions per Run 1 per chip
Block Format 20,000 partitions/chip
Color Combinations (Multiplexing) 2 (endpoint detection)
Run Time ~30 seconds to read 1 chip
Programmable Volumes N/A
Validated volumes 14.5 μL per chip
Automation Compatible No
Remote Monitoring No
On-Board Memory Yes, >500 chips
Email Notification No
Assist with 21 CFR Part 11 Compliance No
Sensitivity Down to 1 copy
Dynamic Range 5 logs
Chemistry TaqMan and SYBR Green
Applications Available Rare Mutation Detection, Quantification of molecular standards, Copy Number Variation, Absolute Quantification, Pathogen Detection,  Digital PCR
IVD Platform No
Temperature Uniformity N/A
Excitation Source OptiFlex System with white LED light
Filters/Colors 3 excitation filters (450-600 nm) 3 emission filters (500-640 nm)
Thermo Fisher Cloud Direct connection
Wi-Fi Connectivity Yes
Size W 13.5 cm
D 23.25 cm
H 21 cm
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Take the quiz

Find the parts you need as well as part numbers for our legacy real-time PCR systems.

Reaction tubes, 96-well and 384-well plates, and adhesive films for real-time PCR.

Looking for a thermal cycler for end-point PCR?

A wide range of software applications to help you get the most from your Applied Biosystems real-time PCR system.

Applied Biosystems Spectral Calibration Kits and TaqMan® RNase P Instrument Verification Plates to help calibrate and verify the performance of your real-time PCR system.