Luminex Technology Multiplex Assays
Multiplexing to save time, money, and sample
Immunoassays based on Luminex xMAP (multi-analyte profiling) technology enable simultaneous detection and quantitation of multiple secreted proteins (cytokines, chemokines, growth factors, etc.) and have been invaluable for the comprehensive study of biological systems. This high-throughput technology produces results comparable to ELISA but with greater efficiency, speed, and dynamic range. Plus, multiplexing is less expensive per target result.
We offer Invitrogen ProcartaPlex multiplex immunoassays and QuantiGene Plex multiplex gene expression assays for the Luminex platform in the form of preconfigured panels. Procartaplex immunoassays are also available as singleplex assays so you can configure your own panel. In addition, we offer and support Luminex instrumentation, including MAGPIX, LX200, and FLEXMAP 3D systems.
ProcartaPlex configurable simplex and multiplex immunoassay panels use Luminex xMAP technology to cover more than 400 cytokine, chemokine, growth factor, and other protein targets from human, mouse, rat, porcine, canine and nonhuman primate species.
The Invitrogen QuantiGene Plex Assay utilizes xMAP Luminex beads for multiplexing of 3 to 80 RNA targets followed by branched DNA-based signal amplification. The assay is performed in a 96-well plate and signal is detected using a Luminex instrument.
Sales and support for MAGPIX, LX200, and FLEXMAP 3D Luminex instrument systems involving detection by Luminex xMAP (multianalyte profiling) technology with Invitrogen ProcartaPlex assays.
We continue to support customers of our pre-existing (non-ProcartaPlex) portfolio of Invitrogen assays for the Luminex platform. Contact us at LuminexFAS@thermofisher.com for personalized assistance. While supplies last, selected legacy singleplex kits and multiplex panels remain available for purchase. Navigate to the following product categories, then search or filter results by the word “Novex”.
QuantiGene RNA assays are hybridization-based assays that utilize a branched DNA technology for signal amplification for the direct quantitation of gene expression transcripts. The QuantiGene Plex and QuantiGene Singleplex RNA assays are designed to quantitatively measure RNA directly from cell or tissue homogenates and even directly from blood without RNA purification or enzymatic manipulation, thereby avoiding inefficiencies inherent to these processes.
The Invitrogen QuantiGene Plex DNA Copy Number Variation Assay is a 96-well, hybridization-based assay that utilizes xMAP Luminex magnetic beads for multiplexing of 3 to 33 DNA targets and branched-DNA signal amplification technology to quantify DNA targets, allowing precise determination of copy number variation. Signal is detected using a Luminex instrument.
The xMAP technology applies to two bead types—magnetic and polystyrene. The magnetic Magplex bead is a superparamagnetic 6.5 micron microsphere with a magnetic core and polystyrene surface. The polystyrene Microplex bead is a 5.6 micron polystyrene microsphere. Both bead types are internally dyed with precise proportions of red and infrared fluorophores. The differing proportions of the red and infrared fluorophores result in 100 unique spectral signature microspheres which are identified by the Luminex xMAP detection systems. The conjugation of a distinct monoclonal antibody to a distinct bead allows for analysis of multiple analytes in a single well.
The polystyrene bead requires expensive, and sometimes difficult to use filter plates. This bead type also makes automating the assay more difficult. The magnetic bead provides significant advantages over the polystyrene bead because it allows for greater ease and flexibility. This ease is realized in the ability to use a flat-bottom plate with either a hand-held magnetic plate washer (cat. no. EPX-55555-00) or an automated magnetic plate washer. In addition, a filter plate/vacuum manifold system, commonly used with polystyrene beads, can also be used with magnetic beads.
Luminex instrumentation is comprised of three instrument models—MAGPIX, LX200 and FLEXMAP 3D. These instruments are based on either the principles of quantitative fluorescent microscopy or fluorescent flow cytometry. The multiplex quantitation capability of an assay is dependent upon instrument model, and ranges from 50 analytes with use of the MAGPIX, to 500 analytes with the FLEXMAP 3D. Used in combination with ProcartaPlex, the Luminex platform delivers fast and cost-effective immunoassay profiling analysis.
The Luminex System is a series of three instruments that flexible analyzer based on the principles of quantitative fluorescent microscopy or fluorescent flow cytometry that is designed to meet the needs of any size research laboratory. The multiplex system allows for the simultaneously measurement of up to 80 different analytes from a single microplate well, using very small sample volumes. The system delivers fast and cost-effective immunoassay profiling and gene expression analysis through the multiplex quantitative measurement and detection of protein, RNA, and DNA.
The Luminex System is the combination of three core xMAP technologies. The first is xMAP microspheres, a family of 100 fluorescently dyed 5.6 magnetic microspheres (beads) and 5.6 micron-sized polystyrene microspheres. Both bead types act as both the target identifier and the solid surface capture to build the assay. The second is a fluorescent imaging and flow cytometry-based instruments, the Luminex Magpix and Luminex 200/FLEXMAP 3D analyzer, respectively, which integrates key xMAP detection components such as light emitting diodes (LEDs) and a CCD camera or lasers, optics, advanced fluidics and high-speed digital signal processors. The third component is the assays that are designed around the microspheres.
More recently Luminex introduced the MAGPIX system which is based on the principles of fluorescence imaging. Lasers and Photo Multiplying Tubes (PMTs) were replaced with Light Emitting Diodes (LEDs) and a CCD camera to deliver a cost effective, compact, and reliable multiplexing platform. The compact MAGPIX instrument is capable of simultaneously measuring and quantitating up to 50 analytes in a single microtiter well when used with magnetic micropsheres (beads) (described above). The MAGPIX instrument is ideal for low to medium-throughput labs for the quantitative analysis of proteins, RNA, and DNA in a variety of sample matrices.
Luminex 200 and FLEXMAP 3D
The Luminex 200 and FLEXMAP 3D reader combines two lasers, fluidics, and real-time digital signal processing to distinguish up to 80 different sets of color-coded magnetic microsphere beads, each bearing a different assay. The Luminex reader is an essential tool that performs the key functions of this multiplex technology:
The reader detects individual beads by flow cytometry. The fluidics system of the reader aligns the beads into single file as they enter a stream of sheath fluid and then enter a flow cell. Once the beads are in single file within the flow cell, each bead is individually interrogated for bead color (analyte) and assay signal strength (PE fluorescence intensity).
The reader uses a 532 nm green laser ("assay" laser) is used to excite the PE dye of the assay (streptavidin-PE). The 635 nm solid state laser (red "classify" laser) is used to excite the dyes inside the beads to determine their "color" or "region" and is also used for doublet discrimination by light scatter.
The reader has four detectors, one for each of the optical paths shown in the figure below. Detectors are used to measure the fluorescence of the assay, to make bead determination (1-100) and the last to discriminate between single and aggregate beads.
Hand-Held magnetic plate washer for magnetic bead assays
We have developed a proprietary hand-held magnetic plate washer (sold separately) to simplify the bead wash process.
This 72-page guide provides detailed information about different tools for protein and RNA quantitation. Download this valuable technical resource that covers technologies useful for cancer and inflammation research, immunology, neurology and more. Learn more about how antibody pairs, ELISA kits, and multiplex kits for the Invitrogen Luminex platform may help advance your research.
For Research Use Only. Not for use in diagnostic procedures.