Membranes for Western Blotting
Choosing your western blot membrane
|Membrane type||Applications & uses*||Reprobe characteristics||Binding interaction|
|Nitrocellulose membranes||WB-low mol wt proteins (<20 kDa), S,N-nucleic acids (<300 bp), AA, DSB||Can be stripped & reprobed||Hydrophobic and electrostatic|
|PVDF membranes (including Invitrolon™ PVDF)||WB, P, AA, SPA||Can be stripped & reprobed||Hydrophobic|
|iBlot® Transfer Stack, Nitrocellulose Membrane
||WB-low mol wt proteins (<20 kDa)||Cannot be stripped & reprobed||Hydrophobic and electrostatic|
|iBlot® Transfer Stack, PVDF Membrane
||WB||Can be stripped & reprobed||Hydrophobic|
|Nylon||S, N, WB||Can be stripped & reprobed||Ionic, hydrophobic, and electrostatic|
*WB=Western blot transfer, S=Southern transfer, N=Northern transfer, AA=Amino acid analysis, DBS=Dot/Slot blotting, P=Protein sequencing, SPA=Solid phase assay systems.
Sheets and rolls of nitrocellulose with binding properties suitable for western blot and other protein or nucleic acid methods.
Sheets and rolls of high-quality polyvinylidene difluoride (PVDF) membrane that offer high protein and nucleic acid binding capacity.
|NEW||Protein Electrophoresis and Western Blotting Support Center
Find tips, troubleshooting help, and resources for your protein expression applications.
For Research Use Only. Not for use in diagnostic procedures.