89839-Lyso-Enrichment-Kit-230

These reagent-based kits are optimized for the isolation and enrichment of organelles, including mitochondria, lysosomes, and synaptosomes. Isolated mitochondria, lysosomes and extracted synaptic proteins are compatible with protein assays and typical downstream applications. These reagents provide high protein yield and enable consistent results due to our stringent controls over ingredient quality and formulation.

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Select a mitochondria isolation kit for your experiment

 
  Mitochondria isolation kit for tissue Mitochondria isolation kit for cultured cells
Compatible sample types Heart and liver tissue Cultured mammalian cells
Organelle isolated Mitochondria Mitochondria
Sample processing time 1 hr 40 min
Mechanical disruption required? Dounce optional Dounce optional
Amount of sample processed 50 samples each containing 50 to 200 mg of soft or hard tissue 50 samples each containing 20 million cultured mammalian cells
Protein assay compatibility BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford BCA, Pierce 660, Detergent Compatible Bradford
Downstream compatibility Western blot, ELISA, amine reactive labeling, apoptosis, signal transduction and metabolic studies Western blot, ELISA, amine reactive labeling, apoptosis, signal transduction and metabolic studies
Protease and/or phosphatase inhibitors recommended? Yes, both Yes, both
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Retain activity while enriching key subcellular components