Streamline your workflow with our primer design tool

From design to synthesis, quality primers are vital to successful results. Use our online Applied Biosystems™ Primer Designer™ Tool to search for the right PCR/Sanger sequencing primer pair from a database of ~650,000 predesigned primer pairs for resequencing the human exome and human mitochondrial genome. Choose from different amplicon lengths to accommodate various research applications and biological sample types.

  • Our primers are free of known SNPs and primer-dimers, highly target-specific and used under universal PCR conditions 
  • Full primer coverage for Ion Torrent™ AmpliSeq™ Exome Panel and Ion Torrent™ AmpliSeq™ Cancer Hotspot Panel v.2 Sanger confirmation workflow
  • Flexible primer configuration to meet your research needs: primers can be ordered unmodified, M13-tailed, HPLC-purified or desalted
  • All the primers have been checked by mass spectrometry and have passed stringent bioinformatics metrics; lab bench validation tests have shown >95% success rate

No time for primer design?

From design to synthesis, quality primers are vital to successful results. Search our tool, to find and order pre-designed primers for PCR and Sanger sequencing. Free of SNPs and primer-dimers, highly target-specific, and used under universal PCR conditions - these primers can be ordered unmodified, M13-tailed, HPLC-purified, or desalted. All are checked by mass spectometry. Have confidence knowing primer pairs are selected to tile across exons for optimal exon coverage with a minimal number of primer pairs.

Save time with the Primer Designer™ Tool by selecting from >300,000 primer pairs targeting the human exome.

Primer Designer Tool – Search for PCR & Sanger Sequencing Primers

(File must be less than 500 kb in size and contain less than 1000 unique line-items.)



Fast & powerful data analysis for your genomics platforms

Learn more about our cloud-based Sanger analysis modules

Product literature

Primer Designer Tool quick reference card
Primer Designer Tool brochure
Primer Designer Tool application note
NGS Confirmation App Note
Human Genome 38 FAQ

Visistat reference component

Primer Designer™ Tool:

Delivery schedule for pre-designed primer pairs.

Learn more

Learn about the Sanger sequencing workflow.

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Access software to align Sanger and NGS sequencing files for result confirmation.

Open VR Toolkit™ software


Primer Designer™ Tool Quick Reference card
Primer Designer™ Tool Product Bulletin
Primer Designer™ Tool FAQs
Aplication Note: Primer Designer™ Tool



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