Our Newest Cellular Analysis Products and Technologies

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Positive Isolation of Monocytes Directly From Blood

Cell isolation directly from whole blood or buffy coat saves time, reduces costs, and minimizes manipulations compared to isolation via PBMC. Gentle Dynabeads® FlowComp™ technology allows for release and removal of the beads from the cells after isolation. CD14+ monocytes show 96–99% purity, high recovery, and increased viability compared to PBMC isolation. Functional responses are kept intact in subsequent experiments, and the cells can be used in any downstream assay, including monocyte-derived dendritic cells (Mo-DC) or cytokine release.

Dynabeads® FlowComp™ Human CD14

Dynabeads® FlowComp™ Human CD14 was used to isolate bead-free monocytes from buffy coat with high purity.

Versatile and Flexible HulaMixer™ Sample Mixer

The multipurpose HulaMixer™ helps mix practically any sample in any tube. It can rotate in three ways: orbital (end-over-end), reciprocal (tilting), and vibration (vortex). The HulaMixer™ is flexible, intuitive, and easy to operate. Adjust the speed (1–100 rpm), time, and movement combinations to match your changing needs. The mixer is small; just carry it to the incubator or cold room (5–40°C).

The HulaMixer™ Sample Mixer  
The versatile HulaMixer™ Sample Mixer includes two platforms to suit all kinds of tubes (0.05–50 mL).

Copper-Free DIBO Alternatives for Detecting Azide-Modified Proteins With Click Chemistry

Classic click chemistry involves the copper-catalyzed triazole formation from an azide and an alkyne. In certain situations, copper can be cytotoxic, reduce or eliminate the fluorescence of Qdot® nanocrystals and fluorescent proteins, and reduce activity of certain enzymes. Our new Click-iT® DIBO reagents now offer a means of achieving click reactions without copper for detection of azide-modified proteins. Nine new Click-iT® products are available, including DIBO derivatives of Alexa Fluor®, TAMRA, and biotin labels, and reactive probes capable of modifying amine, cysteine, and carboxy groups.

Copper-free click azide/DIBO reaction

Copper-free click azide/DIBO reaction. The azide and DIBO moieties are interchangeable; the molecule can be labeled with a DIBO and reacted with a fluorophore or hapten-azide.

APEX® Alexa Fluor® 568 and Biotin-XX Labeling Kits

APEX® Antibody Labeling Kits provide a convenient means of directly labeling a small amount of IgG antibody (10–20 µg) with a fluorescent molecule or biotin-XX. The kits use a solid-phase labeling technique that captures the IgG antibody on the resin inside the APEX® antibody labeling tip in as little as 2.5 hours. Additional kits for Alexa Fluor® 568 and biotin-XX antibody labeling are now available.

s007839_APEX-Labeling Kits
Labeling of α-tubulin in bovine pulmonary artery endothelial (BPAE) cells with the APEX® Biotin-XX Antibody Labeling Kit (left panel) or Alexa Fluor® 568 Antibody Labeling Kit (right panel).

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.