Multiparameter apoptosis assay.

Control (top row) and 4-hour camptothecin-treated (bottom row) Jurkat cells were stained with 1 µM SYTOX® AADvanced™ Dead Cell Stain (S10349), 200 nM F2N12S (from A35137), and 50 nM MitoProbe™ DiIC1(5) (M34151). Dead cells were first excluded (histograms) by gating live cells (those that have lower SYTOX® AADvanced™ fluorescence, indicated by bars). Bivariant density plots show a two-parameter apoptosis assay for mitochondrial membrane potential loss (decreased DiIC1(5) fluorescence) and breakdown of membrane asymmetry (smaller F2N12S 585/530 nm fluorescence ratio). The A, L, and D labels on the graphs indicate apoptotic, live, and dead cells, respectively.

Control (top row) and 4-hour camptothecin-treated (bottom row) Jurkat cells were stained with 1 µM SYTOX® AADvanced™ Dead Cell Stain (S10349), 200 nM F2N12S (from A35137), and 50 nM MitoProbe™ DiIC1(5) (M34151). Dead cells were first excluded (histograms) by gating live cells (those that have lower SYTOX® AADvanced™ fluorescence, indicated by bars). Bivariant density plots show a two-parameter apoptosis assay for mitochondrial membrane potential loss (decreased DiIC1(5) fluorescence) and breakdown of membrane asymmetry (smaller F2N12S 585/530 nm fluorescence ratio). The A, L, and D labels on the graphs indicate apoptotic, live, and dead cells, respectively.

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