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CD38 / cyclic ADP-ribose hydrolase Antibody (MA1-182) in WB
Western blot analysis of CD38 was performed by loading 50ug of the indicated cell lysates prepared using IP Lysis Buffer (Product ## 87788) or Mem-PER Plus Membrane Protein Extraction Kit (Product ## 89842), and 10ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202). Proteins were transferred to a nitrocellulose membrane (Product ## 88014) using the Power Blotter (Product ## 22834), and blocked with 5% milk in TBST for at least 1 hour at room temperature. CD38 was detected at ~45kD using a CD38 monoclonal antibody (Product ## MA1-182) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG secondary antibody (Product ## 31430) at a dilution of 1:40,000 for at least 30 minutes. Chemiluminescent detection was performed using SuperSignal West Dura (Product ## 34076).
