HeLa cell Golgi and cytoskeleton imaged using the EVOS® FL Auto imaging system

HeLa cells were transduced with CellLight® Nucleus-GFP (nucleus, green, C10602), grown over night. Following fixation, permeabilization and block using the Image-iT Fixation/Permeabilization Kit (R37602), the cystoskeleton was stained using ActinRed™ 555 ReadyProbes® reagent (actin, red, R37112). Nuclei were counter-stained with NucBlue® Fixed cell stain (R37606) and images were acquired on the EVOS® FL Auto imaging system using a 20x objective (AMEP4624).

HeLa cells were transduced with CellLight® Nucleus-GFP (nucleus, green, C10602), grown over night. Following fixation, permeabilization and block using the Image-iT Fixation/Permeabilization Kit (R37602), the cystoskeleton was stained using ActinRed™ 555 ReadyProbes® reagent (actin, red, R37112). Nuclei were counter-stained with NucBlue® Fixed cell stain (R37606) and images were acquired on the EVOS® FL Auto imaging system using a 20x objective (AMEP4624).

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