{"id":1072,"date":"2026-03-23T07:29:27","date_gmt":"2026-03-23T07:29:27","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1072"},"modified":"2026-04-22T21:59:28","modified_gmt":"2026-04-22T21:59:28","slug":"phosphorylated-proteins","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/phosphorylated-proteins\/","title":{"rendered":"\u6d41\u5f0f\u68c0\u6d4b\u7ec6\u80de\u5185\u78f7\u9178\u5316\u86cb\u767d"},"content":{"rendered":"\n

\u4ec0\u4e48\u662f\u86cb\u767d\u78f7\u9178\u5316\uff1f<\/strong><\/h2>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u56fe1. \u78f7\u9178\u5316\u4fe1\u53f7\u7f51[1]<\/sup><\/p>\n\n\n\n

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\u4f20\u7edf\u68c0\u6d4b\u65b9\u6cd5\u5982Western Blot<\/strong>\u548c2D\u7535\u6cf3<\/strong>\u5728\u9ad8\u901a\u91cf\u53ca\u5355\u7ec6\u80de\u6c34\u5e73\u68c0\u6d4b\u5b58\u5728\u5c40\u9650\u3002\u6d41\u5f0f\u7ec6\u80de\u672f\u5c31\u50cf\u4e00\u4e2a\u8d85\u7ea7\u9ad8\u6548\u7684\u5206\u62e3\u673a\u5668\u4eba\uff0c\u5229\u7528\u7279\u5f02\u6027\u78f7\u9178\u5316\u6297\u4f53\u8fd9\u4e2a\u6293\u624b\uff0c\u80fd\u5728\u51e0\u5206\u949f\u5185\u4ece\u6210\u5343\u4e0a\u4e07\u4e2a\u7ec6\u80de\u4e2d\u627e\u5230\u78f7\u9178\u5316\u86cb\u767d\uff0c\u8fdb\u884c\u9ad8\u901a\u91cf\u53ca\u5355\u7ec6\u80de\u6c34\u5e73\u68c0\u6d4b\uff0c\u6781\u5927\u63d0\u5347\u4e86\u68c0\u6d4b\u7684\u7075\u654f\u5ea6<\/strong>\u548c\u51c6\u786e\u6027<\/strong>\u3002<\/p>\n\n\n\n

\u5982\u4f55\u7528\u6d41\u5f0f\u68c0\u6d4b\u86cb\u767d\u78f7\u9178\u5316\uff1f<\/strong><\/h2>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u6837\u54c1\u5236\u5907\u30102\u3011<\/sup><\/strong><\/h2>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

Red\uff1auntreated\uff0cBlue\uff1atreated[3]<\/sup><\/p>\n\n\n\n

\u6837\u54c1\u56fa\u5b9a\u548c\u901a\u900f\uff0c\u4f7f\u7528\u8367\u5149\u6807\u8bb0\u7684\u78f7\u9178\u5316\u6297\u4f53\u67d3\u8272\u30102\u3011<\/sup><\/strong><\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

Red\uff1auntreated\uff0cBlue\uff1atreated (blue histogram) with H2<\/sub>O2<\/sub>-activated sodium pervanadate for 5 minutes at 37\u00b0C. [3]<\/sup><\/p>\n\n\n\n

\u6d41\u5f0f\u4e0a\u673a\u548c\u6570\u636e\u5206\u6790\u30102\u3011<\/sup><\/strong><\/h3>\n\n\n\n

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\"\"<\/figure>\n\n\n\n

\u5b9e\u9a8c\u6b65\u9aa4<\/strong><\/h2>\n\n\n\n

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    \n
  1. \u5728\u9002\u5f53\u7684\u57f9\u517b\u57fa\u4e2d\u57f9\u517b\u76ee\u6807\u7ec6\u80de\u7528\u4e8e\u523a\u6fc0\u3002<\/li>\n\n\n\n
  2. \u8ba1\u6570\u7ec6\u80de\uff0c\u5728\u9002\u5f53\u7684\u57f9\u517b\u57fa\u4e2d\u91cd\u60ac\u81f31-5\u00d710\u2076\u4e2a\u7ec6\u80de\/mL\u3002<\/li>\n\n\n\n
  3. 37\u00b0C\u4e0b\uff0c\u9009\u7528\u9002\u5f53\u7684\u65f6\u95f4\u70b9\u523a\u6fc0\u7ec6\u80de\u3002\u540c\u65f6\u5b75\u80b2\u672a\u5904\u7406\u7684\u7ec6\u80de\uff0c\u4f5c\u4e3a\u9634\u6027\u5bf9\u7167\u3002<\/li>\n\n\n\n
  4. [\u53ef\u9009] \u56fa\u5b9a\u4e4b\u524d\u8fdb\u884c\u8868\u9762\u67d3\u8272\uff0c\u4f7f\u7528\u8010\u7532\u9187\u8367\u5149\u67d3\u6599\u5076\u8054\u7684\u6297\u4f53\u3002<\/li>\n\n\n\n
  5. \u5728\u523a\u6fc0\u7ed3\u675f\u65f6\uff0c\u5728\u7ec6\u80de\u4e2d\u52a0\u5165\u7b49\u4f53\u79ef\u7684\u6d41\u5f0f\u80de\u5185\u56fa\u5b9a\u6db2\uff08\u8d27\u53f7# 00-8222\uff09\u5e76\u6da1\u65cb\u632f\u8361\uff0c\u56fa\u5b9a\u7ec6\u80de\u4ee5\u7ec8\u6b62\u523a\u6fc0\u3002<\/li>\n\n\n\n
  6. \u5ba4\u6e29\u907f\u5149\u5b75\u80b2\u7ec6\u80de10-60 min\u3002<\/li>\n\n\n\n
  7. \u5ba4\u6e29600 x g\u79bb\u5fc3\u7ec6\u80de4-5 min\uff0c\u5f03\u4e0a\u6e05\u3002<\/li>\n\n\n\n
  8. \u7528\u6b8b\u7559\u4f53\u79ef\u91cd\u60ac\u7ec6\u80de\uff0c\u52a0\u51651 mL\u9884\u51b7\u768490-100\uff05\u7532\u9187\uff0c\u6da1\u65cb\u632f\u8361\uff0c\u7f6e\u4e8e4\u00b0C\u6216\u51b0\u4e0a\u5b75\u80b2\u81f3\u5c1130 min\u3002<\/li>\n\n\n\n
  9. \u7528\u8fc7\u91cf\u4f53\u79ef\u7684\u6d41\u5f0f\u7ec6\u80de\u67d3\u8272\u6db2\u6d17\u6da4\u7ec6\u80de\u3002<\/li>\n\n\n\n
  10. \u5ba4\u6e29600 x g\u79bb\u5fc3\u7ec6\u80de4-5 min\uff0c\u5f03\u4e0a\u6e05\u3002<\/li>\n\n\n\n
  11. \u7528\u6d41\u5f0f\u7ec6\u80de\u67d3\u8272\u6db2\u4ee51×10\u2077\u4e2a\u7ec6\u80de\/mL\u91cd\u60ac\u7ec6\u80de\uff0c\u5e76\u5c061×10\u2076\u4e2a\u7ec6\u80de\uff08100 \u03bcL\uff09\u7b49\u5206\u5230\u5355\u72ec\u7684\u6d41\u5f0f\u7ba1\u4e2d\u3002<\/li>\n\n\n\n
  12. [\u53ef\u9009] \u67d3\u8272\u524d\uff0c\u4f7f\u7528\u7eaf\u5316\u7684\u6297\u5c0f\u9f20CD16\/CD32\uff08\u8d27\u53f7# 14-016\uff09\u6216\u4ebaFc\u53d7\u4f53\u7ed3\u5408\u6291\u5236\u5242\uff08\u8d27\u53f7# 14-9161\uff09\u5c01\u95ed\u3002<\/li>\n\n\n\n
  13. \u6bcf\u7ba1\u52a0\u5165\u63a8\u8350\u91cf\u7684\u8367\u5149\u6807\u8bb0\u6297\u4f53\uff0c\u5ba4\u6e29\u907f\u5149\u5b75\u80b230-60 min\uff0c\u3002<\/li>\n\n\n\n
  14. \u52a0\u51652 mL\u6d41\u5f0f\u7ec6\u80de\u672f\u67d3\u8272\u6db2\uff0c\u5ba4\u6e29600 x g\u79bb\u5fc34-5 min\uff0c\u5f03\u4e0a\u6e05\u3002<\/li>\n\n\n\n
  15. \u91cd\u590d\u7b2c14\u6b65\u3002<\/li>\n\n\n\n
  16. \u5c06\u67d3\u8272\u7ec6\u80de\u91cd\u60ac\u4e8e\u9002\u5f53\u4f53\u79ef\u7684\u6d41\u5f0f\u7ec6\u80de\u672f\u67d3\u8272\u6db2\u4e2d\uff0c\u6d41\u5f0f\u7ec6\u80de\u4eea\u4e0a\u673a\u5206\u6790\u3002<\/li>\n<\/ol>\n\n\n\n

    \u5e38\u89c1Question & Answer<\/strong><\/h2>\n\n\n\n

    Q \u6211\u8d2d\u4e70\u4e86\u4e00\u53ea\u78f7\u9178\u5316\u6297\u4f53\u8981\u505a\u6d41\u5f0f\u68c0\u6d4b\uff0c\u8bf7\u95ee\u8981\u7528\u54ea\u79cd\u56fa\u5b9a\u548c\u901a\u900f\u65b9\u6cd5\uff1f<\/strong><\/p>\n\n\n\n

    A<\/strong> \u9488\u5bf9\u4e0d\u540c\u9776\u70b9\u7684\u78f7\u9178\u5316\u6297\u4f53\uff0c\u8bf7\u53c2\u8003\u6297\u4f53\u7684\u8bf4\u660e\u4e66\u4e0a\u63a8\u8350\u7684\u56fa\u5b9a\u548c\u901a\u900f\u6761\u4ef6\u3002\u6211\u4eec\u5bf9\u4e8e\u5f88\u591a\u78f7\u9178\u5316\u6297\u4f53\u6d4b\u8bd5\u4e86\u4e0a\u9762\u63d0\u53ca\u4e09\u79cd\u56fa\u5b9a\u901a\u900f\u6761\u4ef6\uff0c\u70b9\u51fb\u4e86\u89e3<\/a>\u66f4\u591a\u4fe1\u606f\u3002<\/p>\n\n\n\n

    Q \u5982\u4f55\u540c\u65f6\u67d3\u8868\u9762\u86cb\u767d\uff1f<\/strong><\/p>\n\n\n\n

    A<\/strong> \u6211\u4eec\u5efa\u8bae\u8868\u9762\u86cb\u767d\u67d3\u8272\u4e0e\u80de\u5185\u78f7\u9178\u5316\u86cb\u767d\u67d3\u8272\u4e00\u8d77\u8fdb\u884c\uff0c\u4f46\u662f\u9700\u8981\u8bc4\u4f30\u5e76\u4f7f\u7528\u53ef\u8bc6\u522b\u56fa\u5b9a\u8868\u4f4d\u7684\u8868\u9762\u86cb\u767d\u7684\u6297\u4f53\u514b\u9686\uff0c\u6211\u4eec\u6d4b\u8bd5\u4e86\u5f88\u591a\u8868\u9762\u86cb\u767d\u6297\u4f53\u514b\u9686\u6027\u80fd\uff0c\u70b9\u51fb\u4e86\u89e3<\/a>\u66f4\u591a\u4fe1\u606f\u3002\u00a0<\/p>\n\n\n\n

    \u8868\u9762\u548c\u80de\u5185\u86cb\u767d\u5206\u5f00\u67d3\u8272\u65f6\u9700\u6ce8\u610f\u9009\u62e9\u8010\u7532\u9187\u8367\u5149\u67d3\u6599\u5076\u8054\u7684\u8868\u9762\u86cb\u767d\u4e00\u6297\uff0c\u5982FITC\uff0cAlexa fluor\u6216eFluor\u7cfb\u5217\u67d3\u6599\u5076\u8054\u7684\u6297\u4f53\u3002<\/p>\n\n\n\n

    \"\"<\/figure>\n\n\n\n

    \u53c2\u8003\u6587\u732e\uff1a<\/h2>\n\n\n\n

    [1]Ardito, F., Giuliani, M., Perrone, D., Troiano, G., & Lo Muzio, L. (2017). The crucial role of protein phosphorylation in cell signaling and its use as targeted therapy.\u202fInternational journal of molecular medicine,\u202f40(2), 271-280. <\/p>\n\n\n\n

    [2]Toney, N. J., Schlom, J., & Donahue, R. N. (2023). Phosphoflow cytometry to assess cytokine signaling pathways in peripheral immune cells: potential for inferring immune cell function and treatment response in patients with solid tumors. Journal of Experimental & Clinical Cancer Research, 42(1), 247. <\/p>\n\n\n\n

    [3]https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/flow-cytometry\/antibodies-for-flow-cytometry\/phospho-specific-antibodies-flow-cytometry.html<\/p>\n\n\n\n

    \"contact<\/a><\/figure>\n\n\n\n

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