{"id":1209,"date":"2026-03-25T07:30:14","date_gmt":"2026-03-25T07:30:14","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=1209"},"modified":"2026-04-22T21:59:27","modified_gmt":"2026-04-22T21:59:27","slug":"fixable-viability-dye-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/fixable-viability-dye-2\/","title":{"rendered":"\u6d41\u5f0f\u53ef\u56fa\u5b9a\u6d3b\u6027\u67d3\u6599"},"content":{"rendered":"\n

Q \u4eb2\u7231\u7684\u540c\u5b66\uff0c\u4f60\u77e5\u9053\u5417\uff1f\u6d41\u5f0fPanel\u4e2d\u8981\u6709\u53bb\u9664\u6b7b\u7ec6\u80de\u7684\u8367\u5149\u67d3\u6599\u3002<\/strong><\/p>\n\n\n\n

A <\/strong>\u5728\u68c0\u6d4b\u7ec6\u80de\u5185\u7ec6\u80de\u56e0\u5b50\u6216\u7ec6\u80de\u6838\u5185\u9776\u6807\u65f6\uff0cPI\u548c7-AAD\u662f\u4e0d\u591f\u7684\uff0c\u8fd9\u8981\u7528\u5230\u53ef\u56fa\u5b9a\u7684\u6d41\u5f0f\u8367\u5149\u67d3\u6599\uff01<\/p>\n\n\n\n

\u8fd9\u4e9b\u90fd\u662f\u975e\u5e38\u91cd\u8981\u7684\u77e5\u8bc6\u70b9\u54e6\uff01\u5728\u8fd9\u91cc\uff0c\u4f60\u53ef\u4ee5\u5feb\u901fGet\u5230Dr.\u8d5b\u4e3a\u4f60\u51c6\u5907\u7684\u6d41\u5f0f\u68c0\u6d4b\u7ec6\u80de\u6d3b\u6027\u57fa\u7840\u77e5\u8bc6\uff0c\u8fd8\u6709\u63a8\u8350\u7684\u53ef\u56fa\u5b9a\u7ec6\u80de\u6d3b\u6027\u67d3\u6599\u597d\u7269\uff01<\/p>\n\n\n\n

\u4e3a\u4ec0\u4e48\u9274\u5b9a\u7ec6\u80de\u6b7b\u6d3b\uff1f<\/strong><\/h2>\n\n\n\n

\u6837\u54c1\u4e2d\u7684\u6b7b\u7ec6\u80de\u4f1a\u7531\u4e8e\u81ea\u53d1\u8367\u5149\u4ee5\u53ca\u975e\u7279\u5f02\u6027\u7684\u6297\u4f53\u6216\u8005\u67d3\u6599\u7ed3\u5408\uff0c\u5bfc\u81f4\u5047\u9633\u6027\u5e76\u589e\u52a0\u80cc\u666f\u4fe1\u53f7\uff0c\u56e0\u6b64\u4ece\u6d41\u5f0f\u5b9e\u9a8c\u6570\u636e\u4e2d\u79fb\u9664\u6b7b\u7ec6\u80de\u662f\u786e\u4fdd\u7ed3\u679c\u7cbe\u786e\u7684\u5173\u952e\u6b65\u9aa4\u3002\u6211\u4eec\u4ee5\u6570\u636e\u8bf4\u8bdd\uff0c\u8bf7\u770b\u56fe\u72471\u4e2d\u7684\u4f8b\u5b50\uff0c\u56fe\u4e2d\u6570\u636e\u501f\u52a9\u7ec6\u80de\u6b7b\u6d3b\u67d3\u6599\u6392\u9664\u4e86\u6b7b\u7ec6\u80de\u7684\u4fe1\u53f7\u5e72\u6270\u540e\uff0c\u5206\u6790\u5f97\u5230\u7684CD8+\u6216\u8005CD19+\u7ec6\u80de\u6bd4\u4f8b\u66f4\u51c6\u786e\u4e86\u3002\u56e0\u6b64\uff0c\u63a8\u8350\u5927\u5bb6\u8fdb\u884c\u6d41\u5f0f\u5b9e\u9a8c\u65f6\uff0c\u5c3d\u91cf\u642d\u914d\u4e0a\u7ec6\u80de\u6b7b\u6d3b\u67d3\u6599\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n
\"\"<\/figure>\n\n\n\n

\u56fe 1. \u7ec6\u80de\u6b7b\u6d3b\u67d3\u6599\u6392\u9664\u6b7b\u7ec6\u80de\u5e72\u6270\u3002<\/strong> \u901a\u8fc7\u5149\u6563\u5c04\u7684\u95e8\u63a7\u5708\u51fa\u76ee\u6807\u6dcb\u5df4\u7ec6\u80de\uff0c\u5206\u6790\u540e\u7684CD8+\u6216\u8005CD19+\u7ec6\u80de\u6bd4\u4f8b\u660e\u663e\u504f\u9ad8\uff08\u4e0a\u56fe\uff09\uff1b\u76f8\u540c\u6837\u54c1\u501f\u52a9\u6b7b\u6d3b\u67d3\u6599\uff0c\u6392\u9664\u4e86\u6b7b\u7ec6\u80de\u7684\u4fe1\u53f7\u5e72\u6270\u540e\uff0c\u5206\u6790\u540e\u7684CD8+\u6216\u8005CD19+\u7ec6\u80de\u6bd4\u4f8b\u66f4\u51c6\u786e\u4e86\uff08\u4e0b\u56fe\uff09\u3002<\/p>\n\n\n\n

\u5982\u4f55\u9009\u62e9\u5408\u9002\u7684\u6b7b\u6d3b\u67d3\u6599\uff1f<\/strong><\/h2>\n\n\n\n

\u4f20\u7edf\u7684\u6d41\u5f0f\u7ec6\u80de\u6b7b\u6d3b\u67d3\u6599\u4e3a\u7ec6\u80de\u819c\u975e\u901a\u900f\u6027\u7684\u6838\u9178\u67d3\u6599\uff0c\u4f8b\u5982PI\u30017-AAD\u3001DAPI\u3002\u8fd9\u7c7b\u6838\u9178\u67d3\u6599\u80fd\u7a7f\u8fc7\u6b7b\u7ec6\u80de\u7684\u7ec6\u80de\u819c\uff0c\u7ed3\u5408\u6838\u9178\u540e\u53d1\u51fa\u8367\u5149\uff0c\u800c\u65e0\u6cd5\u7a7f\u8fc7\u6d3b\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u3002<\/p>\n\n\n\n

\u4f46\u662f\uff0c\u5982\u679c\u5b9e\u9a8c\u6d89\u53ca\u5230\u80de\u5185\u67d3\u8272\uff0c\u7ec6\u80de\u9700\u8981\u56fa\u5b9a\u7834\u819c\uff0c\u60a8\u53ef\u80fd\u4f1a\u9047\u5230\u56fe2\u4e0a\u56fe\u8fd9\u79cd\u60c5\u51b5\uff0c\u6838\u9178\u67d3\u6599\u4f1a\u5c06\u6240\u6709\u7684\u7ec6\u80de\u90fd\u67d3\u4e0a\u989c\u8272\uff0c\u96be\u4ee5\u5206\u8fa8\u6b7b\u6d3b\u4fe1\u53f7\u3002\u6b64\u65f6\u60a8\u5e94\u8be5\u9009\u62e9\u53ef\u4ee5\u56fa\u5b9a\u7684\u7ec6\u80de\u6b7b\u6d3b\u67d3\u6599\uff0c\u5373Fixable viability dye\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe 2. \u56fa\u5b9a\u540e LIVE\/DEAD\u2122 Fixable dye\u4fe1\u53f7\u53ef\u4fdd\u7559\u3002<\/strong>\u5206\u522b\u7528PI\u6216LIVE\/DEAD\u2122 Fixable Stain\u6807\u8bb0\u7ec6\u80de\uff0cPI\u6807\u8bb0\u7684\u7ec6\u80de\u56fa\u5b9a\u540e\u96be\u4ee5\u5206\u8fa8\u6b7b\u6d3b\u4fe1\u53f7\uff08\u4e0a\u56fe\uff09\uff0cLIVE\/DEAD\u2122 Fixable dye\u5728\u56fa\u5b9a\u540e\u7ef4\u6301\u4e86\u6b7b\u7ec6\u80de\u548c\u6d3b\u7ec6\u80de\u7684\u533a\u522b\uff08\u4e0b\u56fe\uff09\u3002<\/p>\n\n\n\n

Fixable viability dye\uff08LIVE\/DEAD\u2122 Fixable dye\uff09\u662f\u4e00\u79cd\u7ec6\u80de\u819c\u975e\u901a\u900f\u6027\u7684\u80fa\u53cd\u5e94\u67d3\u6599\uff0c \u8fd9\u4e9b\u67d3\u6599\u53ea\u80fd\u7a7f\u8fc7\u7ec6\u80de\u819c\u53d7\u635f\u7684\u7ec6\u80de\uff0c\u4e0e\u7ec6\u80de\u8868\u9762\u6216\u8005\u7ec6\u80de\u5185\u7684\u6e38\u79bb\u80fa\u57fa\u5f62\u6210\u5171\u4ef7\u7ed3\u5408\uff08\u89c1\u56fe3\uff09\u3002\u4e14\u7531\u4e8e\u5171\u4ef7\u5076\u8054\u6bd4\u8f83\u7a33\u5b9a\uff0c\u56e0\u6b64\u5bf9\u7ec6\u80de\u67d3\u8272\u540e\u53ef\u4ee5\u517c\u5bb9\u919b\u7c7b\u56fa\u5b9a\u548c\u7834\u819c\u5904\u7406\uff0c\u67d3\u8272\u7ed3\u679c\u4e0d\u53d7\u5f71\u54cd\uff08\u89c1\u56fe2\u4e0b\u56fe\uff09\u3002<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

\u56fe3. LIVE\/DEAD \u53ef\u56fa\u5b9a\u6b7b\u7ec6\u80de\u67d3\u8272\u539f\u7406\u3002<\/strong> \u975e\u7ec6\u80de\u901a\u900f\u7684\u80fa\u53cd\u5e94\u67d3\u6599\u4ec5\u80fd\u7ed3\u5408\u5230\u6d3b\u7ec6\u80de\u7684\u8868\u9762\uff0c\u4ea7\u751f\u975e\u5e38\u5fae\u5f31\u7684\u8367\u5149\u3002\u8fd9\u4e9b\u67d3\u6599\u53ef\u7a7f\u900f\u6b7b\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u5e76\u4e0e\u5185\u90e8\u7684\u86cb\u767d\u53d1\u751f\u7ed3\u5408\uff0c\u4ece\u800c\u4ea7\u751f\u6781\u5f3a\u7684\u8367\u5149\u3002<\/p>\n\n\n\n

\u4e0b\u8868\u662f\u4e24\u79cd\u67d3\u6599\u7684\u9009\u62e9\u6307\u5357<\/strong><\/h3>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Question & Answer<\/strong><\/h2>\n\n\n\n

Q \u4f7f\u7528Fixable viability dye\u65f6\uff0c\u67d3\u8272\u6db2\u6709\u4ec0\u4e48\u8981\u6c42\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u5f53\u4f7f\u7528\u53ef\u56fa\u5b9a\u7684\u7ec6\u80de\u6d3b\u6027\u67d3\u6599\u65f6\uff0cTris\u7f13\u51b2\u6db2\u548c\u542b\u6709\u53e0\u6c2e\u5316\u94a0\u6216\u5916\u6765\u86cb\u767d\u8d28\u7684\u6eb6\u6db2\u4e0d\u80fd\u4f5c\u4e3a\u7ec6\u80de\u67d3\u8272\u7f13\u51b2\u6db2\uff0c\u56e0\u4e3a\u6eb6\u6db2\u4e2d\u542b\u6709\u8fd9\u4e9b\u7269\u8d28\u53ef\u80fd\u5bfc\u81f4\u6b7b\u7ec6\u80de\u7fa4\u67d3\u8272\u5f3a\u5ea6\u7684\u663e\u8457\u964d\u4f4e\u548c\/\u6216\u6d3b\u7ec6\u80de\u7fa4\u80cc\u666f\u67d3\u8272\u7684\u589e\u52a0\u3002<\/p>\n\n\n\n

Q \u6d41\u5f0f\u5b9e\u9a8c\u4e2d\uff0c\u5229\u7528LIVE\/DEAD Fixable dead cell stain\u6216\u8005\u662fFixable Viability Dye \u80fa\u57fa\u53cd\u5e94\u67d3\u6599\u9274\u5b9a\u6b7b\u6d3b\u7ec6\u80de\uff0c\u9700\u5982\u4f55\u5236\u5907\u8865\u507f\u5355\u67d3\u7ba1\uff1f<\/strong><\/p>\n\n\n\n

A <\/strong>\u8865\u507f\u6837\u672c\u4e2d\u9700\u8981\u540c\u65f6\u68c0\u6d4b\u5230\u9633\u6027\u4fe1\u53f7\u548c\u9634\u6027\u4fe1\u53f7\uff0c\u6211\u4eec\u63d0\u4f9b\u4e24\u79cd\u65b9\u6848\uff1a<\/p>\n\n\n\n

    \n
  1. \u51c6\u5907\u6d3b\u7ec6\u80de\u4e0e\u6b7b\u7ec6\u80de\u7684\u6df7\u5408\u6837\u672c\uff1a\u5c06\u4e00\u4efd\u6d3b\u7ec6\u80de\u6837\u672c\u5e73\u5747\u5206\u6210\u4e24\u4efd\uff0c\u4e00\u4efd\u4e0d\u505a\u5904\u7406\uff0c\u4e00\u4efd\u5236\u5907\u6210\u6b7b\u7ec6\u80de\uff0c\u5236\u5907\u5b8c\u6210\u540e\u5c06\u4e24\u4efd\u6837\u672c\u91cd\u65b0\u6df7\u5408\uff0c\u518d\u8fdb\u884c\u67d3\u8272\u3002\u5236\u5907\u6b7b\u7ec6\u80de\u7684\u65b9\u6cd5\u8bf7\u53c2\u8003\u5982\u4e0b\u63cf\u8ff0\uff1a\u53ef\u4ee5\u901a\u8fc760\u00b0C\u653e\u7f6e20\u5206\u949f\u70ed\u706d\u6d3b\u7ec6\u80de\u3002\u6216\u5c06\u7ec6\u80de\u653e\u516570%\u4e59\u9187\u56fa\u5b9a\uff0c\u4e59\u9187\u56fa\u5b9a\u7684\u7ec6\u80de\u53ef\u4ee5\u4fdd\u5b58\u5728-20\u00b0C\uff0c\u4f7f\u7528\u524d\u518d\u53d6\u51fa\u3002<\/li>\n\n\n\n
  2. \u5229\u7528ArC\u2122 \u80fa\u53cd\u5e94\u8865\u507f\u73e0\u8bd5\u5242\u76d2\uff08\u8d27\u53f7A10346\uff09\uff0c\u8be5\u4ea7\u54c1\u53ef\u4e0e\u5177\u6709\u80fa\u53cd\u5e94\u6027\u7684LIVE\/DEAD Fixable dead cell stain\u6216\u8005\u662fFixable Viability Dye\u53ef\u56fa\u5b9a\u6b7b\u7ec6\u80de\u67d3\u6599\u7ed3\u5408\uff0c\u4ee5\u7528\u4e8e\u6d41\u5f0f\u8865\u507f\u8c03\u8282\uff0c\u800c\u65e0\u9700\u81ea\u5df1\u5236\u5907\u8865\u507f\u6837\u672c\u3002<\/li>\n<\/ol>\n\n\n\n

    Q Fixable Viability Dye\u6d41\u5f0f\u67d3\u8272\u7ed3\u679c\u5982\u4f55\u89e3\u8bfb\uff1f<\/strong><\/p>\n\n\n\n

    A <\/strong>\u76f8\u5bf9\u4e8e\u672a\u67d3\u8272\u7684\u7ec6\u80de\uff0cFixable Viability Dye\u67d3\u8272\u7684\u7ec6\u80de\u6574\u4f53\u90fd\u4f1a\u68c0\u6d4b\u5230\u8367\u5149\u4fe1\u53f7\uff0c\u4f46\u662f\u53ef\u4ee5\u901a\u8fc7\u4fe1\u53f7\u7684\u5f3a\u5f31\u6765\u533a\u5206\u6d3b\u7ec6\u80de\u548c\u6b7b\u7ec6\u80de\u3002\u5176\u4e2d\u6d3b\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u5b8c\u6574\uff0c\u4ec5\u7ec6\u80de\u8868\u9762\u86cb\u767d\u80fa\u57fa\u7ed3\u5408\u4e86\u67d3\u6599\uff0c\u5448\u73b0\u8f83\u5f31\u7684\u8367\u5149\u4fe1\u53f7\uff1b\u800c\u6b7b\u7ec6\u80de\u7684\u7ec6\u80de\u819c\u901a\u900f\u6027\u6539\u53d8\uff0c\u67d3\u6599\u80fd\u591f\u8fdb\u5165\u6b7b\u7ec6\u80de\uff0c\u4e0e\u7ec6\u80de\u5185\u548c\u7ec6\u80de\u8868\u9762\u86cb\u767d\u4e0a\u7684\u80fa\u57fa\u7ed3\u5408\uff0c\u56e0\u6b64\u6b7b\u7ec6\u80de\u5448\u73b0\u66f4\u5f3a\u7684\u8367\u5149\u4fe1\u53f7\u3002\uff08\u89c1\u56fe4\uff09<\/p>\n\n\n\n

    \"\"<\/figure>\n\n\n\n

    \u56fe4. Fixable Viability Dye\u6d41\u5f0f\u67d3\u8272\u7ed3\u679c\u89e3\u8bfb\u3002\u5c06\u672a\u67d3\u8272\u7ec6\u80de\u548cFixable Viability Dye\u67d3\u8272\u7ec6\u80de\u6d41\u5f0f\u5206\u6790\u7ed3\u679c\u5408\u5e76\u5206\u6790\u3002\u76f8\u5bf9\u4e8e\u672a\u67d3\u8272\u7684\u7ec6\u80de\uff0cFixable Viability Dye\u67d3\u8272\u7684\u7ec6\u80de\u6574\u4f53\u90fd\u4f1a\u68c0\u6d4b\u5230\u8367\u5149\u4fe1\u53f7\uff0c\u5176\u4e2d\u6b7b\u7ec6\u80de\u5448\u73b0\u6bd4\u8f83\u5f3a\u7684\u8367\u5149\u4fe1\u53f7\uff0c\u800c\u6d3b\u7ec6\u80de\u5448\u73b0\u7684\u4fe1\u53f7\u8f83\u5f31\u3002<\/p>\n\n\n\n

    Q \u6211\u53ef\u4ee5\u5c06\u6d41\u5f0fFixable Viability Dye\u67d3\u8272\u7684\u7ec6\u80de\u901a\u8fc7\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u7ec6\u80de\u6b7b\u6d3b\u5417\uff1f<\/p>\n\n\n\n

    A \u4e0d\u5efa\u8bae\uff0c\u8be5\u67d3\u6599\u5bf9\u57fa\u4e8e\u6210\u50cf\u7684\u5b9e\u9a8c\u6548\u679c\u4e0d\u597d\uff0c\u7531\u4e8e\u6240\u6709\u7ec6\u80de\u90fd\u88ab\u67d3\u8272\uff0c\u5f88\u96be\u901a\u8fc7\u663e\u5fae\u955c\u4ece\u6697\u6de1\u7684\u6d3b\u7ec6\u80de\u4e2d\u5206\u8fa8\u51fa\u660e\u4eae\u7684\u6b7b\u7ec6\u80de\u3002<\/p>\n\n\n\n

    \"contact<\/a><\/figure>\n\n\n\n

    <\/p>\n","protected":false},"excerpt":{"rendered":"

    Q \u4eb2\u7231\u7684\u540c\u5b66\uff0c\u4f60\u77e5\u9053\u5417\uff1f\u6d41\u5f0fPanel\u4e2d\u8981\u6709\u53bb\u9664\u6b7b\u7ec6\u80de\u7684\u8367\u5149\u67d3\u6599\u3002 A 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