{"id":21,"date":"2026-01-08T06:12:26","date_gmt":"2026-01-08T06:12:26","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=21"},"modified":"2026-05-28T06:40:12","modified_gmt":"2026-05-28T06:40:12","slug":"gibson-assembly-101-expert-cloning-tips-you-need-to-know","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/gibson-assembly-101-expert-cloning-tips-you-need-to-know\/","title":{"rendered":"Gibson \u7ec4\u88c5 101\uff1a\u60a8\u9700\u8981\u4e86\u89e3\u7684\u4e13\u4e1a\u514b\u9686\u6280\u5de7"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/communities-social\/socialhub\/blog-subscribe.html?icid=cn-blog-subscribe\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1024x176.jpg\" alt=\"\" class=\"wp-image-2271\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-large\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/GA-101-1-1024x668.png\" alt=\"Gibson Assembly 101: Expert Cloning Tips You Need to Know\" class=\"wp-image-4628\" \/><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Gibson Assembly\u00ae\uff08\u5409\u5e03\u68ee\u7ec4\u88c5\uff09\u6216\u79f0\u5409\u5e03\u68ee\u514b\u9686\uff0c\u662f\u4e00\u79cd\u5f3a\u5927\u7684\u5206\u5b50\u514b\u9686\u6280\u672f\uff0c\u662f\u4f20\u7edf\u9650\u5236\u6027\u5185\u5207\u9176\u65b9\u6cd5\u7684\u4e00\u79cd\u6d41\u884c\u66ff\u4ee3\u65b9\u6848\u3002<\/p>\n\n\n\n<p>\u901a\u8fc7\u5355\u7ba1\u6052\u6e29\u53cd\u5e94\uff0cGibson Assembly\u80fd\u591f\u65e0\u7f1d\u8fde\u63a5DNA\u7247\u6bb5\u3002\u5b83\u7279\u522b\u9002\u7528\u4e8e\u4ece\u591a\u4e2a\u7247\u6bb5\u6784\u5efa\u5927\u578b\u6216\u590d\u6742\u7684\u7ed3\u6784\uff0c\u5e76\u4e14\u53ef\u80fd\u6bd4\u5176\u4ed6\u514b\u9686\u65b9\u6cd5\u66f4\u5feb\u3002<\/p>\n\n\n\n<p>\u65e0\u8bba\u60a8\u662f\u4e00\u4f4d\u7ecf\u9a8c\u4e30\u5bcc\u7684\u7814\u7a76\u4eba\u5458\uff0c\u8fd8\u662f\u521a\u63a5\u89e6Gibson Assembly\u5b9e\u9a8c\u65b9\u6848\u7684\u65b0\u624b\uff0c\u672c\u6307\u5357\u90fd\u6c47\u96c6\u4e86\u6211\u4eec\u5206\u5b50\u751f\u7269\u5b66\u9886\u57df\u4e13\u5bb6\u7814\u53d1\u79d1\u5b66\u5bb6\u7684\u7ecf\u9a8c\u4e0e\u5fc3\u5f97\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cloning\/seamless-cloning-and-genetic-assembly\/gibson-assembly.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\">\u63a2\u7d22<strong> GeneArt Gibson Assembly \u514b\u9686\u6280\u672f<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-yoast-seo-table-of-contents yoast-table-of-contents\"><h2>\u76ee\u5f55<\/h2><ul><li><a href=\"#h-\u4ec0\u4e48\u662fgibson\u7ec4\u88c5\" data-level=\"2\">\u4ec0\u4e48\u662fGibson\u7ec4\u88c5\uff1f<\/a><\/li><li><a href=\"#h-\u4f55\u65f6\u4ee5\u53ca\u4e3a\u4f55\u4f7f\u7528gibson-assembly\u8fdb\u884c\u5206\u5b50\u514b\u9686\" data-level=\"2\">\u4f55\u65f6\u4ee5\u53ca\u4e3a\u4f55\u4f7f\u7528Gibson Assembly\u8fdb\u884c\u5206\u5b50\u514b\u9686<\/a><\/li><li><a href=\"#h-gibson-assembly\u5b9e\u9a8c\u65b9\u6848\" data-level=\"2\">Gibson Assembly\u5b9e\u9a8c\u65b9\u6848<\/a><ul><li><a href=\"#h-1-\u83b7\u53d6dna\u7247\u6bb5\" data-level=\"3\">1. \u83b7\u53d6DNA\u7247\u6bb5<\/a><\/li><li><a href=\"#h-2-\u6267\u884cgibson\u53cd\u5e94\" data-level=\"3\">2. \u6267\u884cGibson\u53cd\u5e94<\/a><\/li><li><a href=\"#h-3-\u8f6c\u5316\u611f\u53d7\u6001\u7ec6\u80de\" data-level=\"3\">3. \u8f6c\u5316\u611f\u53d7\u6001\u7ec6\u80de<\/a><\/li><li><a href=\"#h-4-\u7b5b\u9009\u6784\u5efa\u4f53\u7684\u83cc\u843d\" data-level=\"3\">4. \u7b5b\u9009\u6784\u5efa\u4f53\u7684\u83cc\u843d<\/a><\/li><\/ul><\/li><li><a href=\"#h-gibson\u7ec4\u88c5\u7684\u6545\u969c\u6392\u9664\u4e0e\u5e38\u89c1\u95ee\u9898\" data-level=\"2\">Gibson\u7ec4\u88c5\u7684\u6545\u969c\u6392\u9664\u4e0e\u5e38\u89c1\u95ee\u9898<\/a><ul><li><a href=\"#h-1-\u5982\u4f55\u52a0\u5feb\u7ec4\u88c5\u8fc7\u7a0b\" data-level=\"3\">1. \u5982\u4f55\u52a0\u5feb\u7ec4\u88c5\u8fc7\u7a0b\uff1f<\/a><\/li><li><a href=\"#h-2-golden-gate\u7ec4\u88c5\u4e0egibson\u7ec4\u88c5-\u6709\u4ec0\u4e48\u533a\u522b-\u6211\u5e94\u8be5\u7528\u54ea\u4e00\u4e2a\u8fdb\u884c\u514b\u9686\" data-level=\"3\">2. Golden Gate\u7ec4\u88c5\u4e0eGibson\u7ec4\u88c5\uff1a\u6709\u4ec0\u4e48\u533a\u522b\uff0c\u6211\u5e94\u8be5\u7528\u54ea\u4e00\u4e2a\u8fdb\u884c\u514b\u9686\uff1f<\/a><\/li><li><a href=\"#h-3-\u5982\u4f55\u8bbe\u8ba1gibson\u7ec4\u88c5\u7684\u5f15\u7269\" data-level=\"3\">3. \u5982\u4f55\u8bbe\u8ba1Gibson\u7ec4\u88c5\u7684\u5f15\u7269\uff1f<\/a><\/li><li><a href=\"#h-4-dna\u7247\u6bb5\u8fdb\u884cgibson\u7ec4\u88c5\u65f6\u5408\u9002\u7684\u91cd\u53e0\u957f\u5ea6\u662f\u591a\u5c11\" data-level=\"3\">4. DNA\u7247\u6bb5\u8fdb\u884cGibson\u7ec4\u88c5\u65f6\u5408\u9002\u7684\u91cd\u53e0\u957f\u5ea6\u662f\u591a\u5c11\uff1f<\/a><\/li><\/ul><\/li><li><a href=\"#h-gibson\u7b49\u6e29\u7ec4\u88c5-\u4e00\u79cd\u4fbf\u6377\u5feb\u901f\u7684\u66ff\u4ee3\u514b\u9686\u65b9\u6cd5\" data-level=\"2\">Gibson\u7b49\u6e29\u7ec4\u88c5\uff1a\u4e00\u79cd\u4fbf\u6377\u5feb\u901f\u7684\u66ff\u4ee3\u514b\u9686\u65b9\u6cd5<\/a><\/li><li><a href=\"#h-\u76f8\u5173\u7684gibson\u7ec4\u88c5\u8d44\u6e90\u4e0e\u6280\u672f\" data-level=\"2\">\u76f8\u5173\u7684Gibson\u7ec4\u88c5\u8d44\u6e90\u4e0e\u6280\u672f<\/a><\/li><\/ul><\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u662fgibson\u7ec4\u88c5\">\u4ec0\u4e48\u662fGibson\u7ec4\u88c5\uff1f<\/h2>\n\n\n\n<p>Gibson\u7ec4\u88c5\u662f\u4e00\u79cd\u5f3a\u5927\u7684DNA\u7ec4\u88c5\u65b9\u6cd5\uff0c\u4f9d\u8d56\u4e8e\u4e09\u79cd\u9176\uff1a\u5916\u5207\u9176\u3001DNA\u805a\u5408\u9176\u548cDNA\u8fde\u63a5\u9176\u3002<\/p>\n\n\n\n<p>\u8be5\u8fc7\u7a0b\u5305\u62ec\u56db\u4e2a\u4e3b\u8981\u9636\u6bb5\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>\u5916\u5207\u9176\u5904\u7406\uff1a<\/strong>\u8be5\u9176\u4eceDNA\u7247\u6bb5\u76845\u2032\u7aef\u5f00\u59cb\u5207\u5272\uff0c\u5f62\u6210\u5355\u94fe\u7a81\u51fa\u672b\u7aef\u3002<\/li>\n\n\n\n<li><strong>\u9000\u706b\uff1a<\/strong>DNA\u7247\u6bb5\u7684\u4e92\u8865\u533a\u57df\u76f8\u4e92\u9000\u706b\u7ed3\u5408\u3002<\/li>\n\n\n\n<li><strong>DNA\u805a\u5408\u9176\u5ef6\u4f38<\/strong>\uff1aDNA\u805a\u5408\u9176\u586b\u8865\u7f3a\u53e3\u3002<\/li>\n\n\n\n<li><strong>\u8fde\u63a5<\/strong>DNA\u8fde\u63a5\u9176\u53ef\u5c01\u95edDNA\u4e3b\u94fe\u4e2d\u7684\u5207\u53e3\uff0c\u4ece\u800c\u5f62\u6210\u8fde\u7eed\u7684DNA\u5206\u5b50\u3002<\/li>\n<\/ol>\n\n\n\n<p>\u4f60\u53ef\u4ee5\u5c06\u6bcf\u4e2aDNA\u7247\u6bb5\u60f3\u8c61\u6210\u4e00\u5757\u5899\u7eb8\u6216\u793c\u54c1\u5305\u88c5\u7eb8\u7684\u788e\u7247\uff1b\u82e5\u60f3\u5c06\u4e24\u5757\u788e\u7247\u8fde\u63a5\u5728\u4e00\u8d77\u800c\u770b\u4e0d\u5230\u63a5\u7f1d\uff0c\u53ef\u4ee5\u5bfb\u627e\u4e24\u4e2a\u788e\u7247\u526a\u5207\u8fb9\u7f18\u5904\u56fe\u6848\u5b8c\u5168\u91cd\u53e0\u7684\u533a\u57df\uff0c\u7136\u540e\u5c06\u5b83\u4eec\u7c98\u5408\u5728\u4e00\u8d77\u3002<\/p>\n\n\n\n<p>\u5728\u8fd9\u4e2a\u7c7b\u6bd4\u4e2d\uff0c\u6bcf\u4e2aDNA\u7247\u6bb5\u672b\u7aef\u7684\u5355\u94fe\u7a81\u51fa\u90e8\u5206\u5c31\u50cf\u56fe\u6848\u7684\u8fb9\u7f18\u2014\u2014\u7531\u5916\u5207\u9176\u5207\u5272\u5f62\u6210\uff0c\u800c\u9000\u706b\u8fc7\u7a0b\u5219\u7c7b\u4f3c\u4e8e\u5bfb\u627e\u89c6\u89c9\u4e0a\u56fe\u6848\u91cd\u53e0\u7684\u8fc7\u7a0b\u3002\u805a\u5408\u9176\u548c\u8fde\u63a5\u53cd\u5e94\u5c06\u7247\u6bb5\u56fa\u5b9a\u5728\u4e00\u8d77\uff0c\u4e0d\u4f1a\u7559\u4e0b\u4efb\u4f55\u53ef\u89c1\u7684\u75d5\u8ff9\u6216\u75a4\u75d5\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/DNA-assembly-workflow.png\" alt=\"Gibson Assembly DNA assembly workflow \" class=\"wp-image-4630\" \/><figcaption class=\"wp-element-caption\"><em><em>DNA\u7ec4\u88c5\u6d41\u7a0b<\/em> <\/em><\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4f55\u65f6\u4ee5\u53ca\u4e3a\u4f55\u4f7f\u7528gibson-assembly\u8fdb\u884c\u5206\u5b50\u514b\u9686\">\u4f55\u65f6\u4ee5\u53ca\u4e3a\u4f55\u4f7f\u7528Gibson Assembly\u8fdb\u884c\u5206\u5b50\u514b\u9686<\/h2>\n\n\n\n<p>\u5409\u5e03\u68ee\uff08Gibson\uff09\u7ec4\u88c5\u6280\u672f\u76f8\u6bd4\u4f20\u7edf\u7684\u9650\u5236\u6027\u5185\u5207\u9176\u514b\u9686\u65b9\u6cd5\u5177\u6709\u4e00\u4e9b\u4f18\u52bf\uff0c\u5305\u62ec\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u80fd\u591f\u540c\u65f6\u8fde\u63a5\u591a\u4e2aDNA\u7247\u6bb5\uff08\u6700\u591a\u53ef\u8fbe6\u4e2a\uff09\uff0c\u65e0\u8bba\u5176\u5e8f\u5217\u5982\u4f55\uff0c\u4e14\u65e0\u9700\u7279\u5b9a\u7684\u9650\u5236\u6027\u9176\u5207\u4f4d\u70b9<\/li>\n\n\n\n<li>\u7247\u6bb5\u4e4b\u95f4\u53ef\u4ee5\u5b9e\u73b0\u7cbe\u786e\u4e14\u65e0\u7f1d\u7684\u8fde\u63a5\uff0c\u4e0d\u4f1a\u4ea7\u751f\u75a4\u75d5<\/li>\n\n\n\n<li>\u76f8\u6bd4\u9650\u5236\u6027\u9176\u5207\u514b\u9686\u65b9\u6cd5\uff0c\u6b65\u9aa4\u66f4\u5c11\u3001\u8017\u65f6\u66f4\u77ed\u2014\u2014\u901a\u5e38\u5728\u4e00\u5c0f\u65f6\u4e4b\u5185\u5373\u53ef\u5b8c\u6210<\/li>\n<\/ul>\n\n\n\n<p>\u5409\u5e03\u68ee\u514b\u9686\u6280\u672f\u5177\u6709\u8bb8\u591a\u5b9e\u9645\u5e94\u7528\uff0c\u53ef\u7528\u4e8e\u65e0\u7f1d\u8d28\u7c92\u6784\u5efa\u3001\u5408\u6210\u57fa\u56e0\u548c\u57fa\u56e0\u7ec4\u7ec4\u88c5\u3001CRISPR\u8f7d\u4f53\u6784\u5efa\u3001\u5b9a\u70b9\u7a81\u53d8\u3001\u6a21\u5757\u5316DNA\u5143\u4ef6\u7ec4\u88c5\uff0c\u4ee5\u53ca\u7528\u4e8e\u57fa\u56e0\u6cbb\u7597\u548c\u7814\u7a76\u7684\u75c5\u6bd2\u8f7d\u4f53\u5f00\u53d1\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa84d8091924&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa84d8091924\" class=\"aligncenter size-full wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/GA-Diagram.jpg\" alt=\"Gibson Assembly applications\" class=\"wp-image-4631\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><em><em>Gibson\u7ec4\u88c5\u5e94\u7528\uff08\u70b9\u51fb\u653e\u5927\u56fe\u7247\uff09<\/em><\/em><\/figcaption><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-gibson-assembly\u5b9e\u9a8c\u65b9\u6848\">Gibson Assembly\u5b9e\u9a8c\u65b9\u6848<\/h2>\n\n\n\n<p>\u8be5 <a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/A46624\" target=\"_blank\" rel=\"noreferrer noopener\">Gibson \u7ec4\u88c5\u514b\u9686\u65b9\u6cd5<\/a>\u662f\u4e00\u79cd\u5355\u6b65\u7b49\u6e29\u8fc7\u7a0b\uff0c\u901a\u5e38\u6240\u9700\u7684\u52a8\u624b\u64cd\u4f5c\u65f6\u95f4\u4e0d\u5230\u4e00\u5c0f\u65f6\uff0c\u5177\u4f53\u65f6\u95f4\u53d6\u51b3\u4e8e\u6240\u6d89\u53ca\u7684DNA\u7247\u6bb5\u6570\u91cf\u3002<\/p>\n\n\n\n<p>\u8be5\u5b9e\u9a8c\u65b9\u6848\u6709\u56db\u4e2a\u5173\u952e\u6b65\u9aa4\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-1-\u83b7\u53d6dna\u7247\u6bb5\">1. \u83b7\u53d6DNA\u7247\u6bb5<\/h3>\n\n\n\n<p>Gibson\u7ec4\u88c5\u7684\u6210\u529f\u4e0e\u5426\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u53d6\u51b3\u4e8eDNA\u7247\u6bb5\u53ca\u5176\u91cd\u53e0\u533a\u57df\u7684\u8bbe\u8ba1\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa84d8091e97&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa84d8091e97\" class=\"aligncenter size-full wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/GAFragments1.png\" alt=\"Illustration of Obtaining DNA Fragments for Gibon Assembly\" class=\"wp-image-4635\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u8bf7\u4ed4\u7ec6\u8bbe\u8ba1\u91cd\u53e0\u5e8f\u5217\"><strong>\u8bf7\u4ed4\u7ec6\u8bbe\u8ba1\u91cd\u53e0\u5e8f\u5217\u3002<\/strong><\/h4>\n\n\n\n<p>\u91cd\u53e0\u533a\u57df\u957f\u5ea6\u5e94\u63a7\u5236\u572820-40\u4e2a\u78b1\u57fa\u5bf9\uff0c\u5e76\u786e\u4fdd\u5176\u5177\u6709\u8f83\u9ad8\u7684GC\u542b\u91cf\u4ee5\u4fc3\u8fdb\u7a33\u5b9a\u7684\u9000\u706b\u7ed3\u5408\u3002 \u53ef\u4ee5\u4f7f\u7528\u5982\u4e0b\u8f6f\u4ef6\u5de5\u5177\uff1a&nbsp;<a href=\"https:\/\/www.snapgene.com\/series\/simulate-gibson-assembly\" target=\"_blank\" rel=\"noreferrer noopener\">SnapGene<\/a>\u2122&nbsp;\u53ef\u7528\u4e8e\u7cbe\u786e\u8bbe\u8ba1\u8fd9\u4e9b\u91cd\u53e0\u533a\u57df\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u4f18\u5316pcr\u6761\u4ef6\"><strong>\u4f18\u5316PCR\u6761\u4ef6<\/strong><\/h4>\n\n\n\n<p>\u4e3a\u4e86\u786e\u4fdd\u9ad8\u8d28\u91cf\u7684PCR\u4ea7\u7269\uff0c\u8bf7\u9488\u5bf9\u6bcf\u4e2aDNA\u7247\u6bb5\u4f18\u5316PCR\u6761\u4ef6\u3002\u4f7f\u7528\u9ad8\u4fdd\u771fDNA\u805a\u5408\u9176\uff0c\u4f8b\u5982Platinum\u2122 SuperFi II PCR\u9884\u6df7\u6db2\uff0c\u4ee5\u51cf\u5c11\u9519\u8bef\u5e76\u786e\u4fdd\u4ea7\u751f\u5e72\u51c0\u3001\u7279\u5f02\u7684\u7247\u6bb5\u3002\u7eaf\u5316PCR\u4ea7\u7269\u4ee5\u83b7\u5f97\u5e72\u51c0\u7684DNA\u7247\u6bb5\uff0c\u5e76\u5728\u8fdb\u884c\u4e0b\u4e00\u6b65\u4e4b\u524d\u901a\u8fc7\u51dd\u80f6\u7535\u6cf3\u9a8c\u8bc1PCR\u4ea7\u7269\u7684\u5b8c\u6574\u6027<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u7ebf\u6027\u5316\u6240\u9009\u8f7d\u4f53\"><strong>\u7ebf\u6027\u5316\u6240\u9009\u8f7d\u4f53<\/strong><\/h4>\n\n\n\n<p>\u9009\u62e9\u9002\u5408\u60a8\u7ec4\u88c5\u9700\u6c42\u7684\u8f7d\u4f53\uff0c\u5e76\u901a\u8fc7\u9650\u5236\u6027\u5185\u5207\u9176\u6216PCR\u5c06\u5176\u7ebf\u6027\u5316\u3002\u7ebf\u6027\u5316\u81f3\u5173\u91cd\u8981\uff0c\u56e0\u4e3a\u5b83\u4e3a\u7247\u6bb5\u7684\u9000\u706b\u548c\u8fde\u63a5\u63d0\u4f9b\u4e86\u5fc5\u8981\u7684\u672b\u7aef\u3002\u5f53\u4f7f\u7528\u73af\u72b6\u8d28\u7c92DNA\u4f5c\u4e3a\u6a21\u677f\u65f6\uff0c\u8bf7\u4f7f\u7528 <a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/FD1703\" target=\"_blank\" rel=\"noreferrer noopener\">DpnI<\/a> \u4ee5\u51cf\u5c11\u8f6c\u5316\u540e\u7684\u6a21\u677f\u80cc\u666f\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-\u6267\u884cgibson\u53cd\u5e94\">2. \u6267\u884cGibson\u53cd\u5e94<\/h3>\n\n\n\n<p>\u8981\u5b9e\u73b0\u6210\u529f\u7684Gibson\u7ec4\u88c5\uff0c\u5fc5\u987b\u4ed4\u7ec6\u4f18\u5316\u53cd\u5e94\u6761\u4ef6\u3002\u53ef\u4ee5\u4f7f\u7528\u8bf8\u5982&nbsp;<a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/A46628\" target=\"_blank\" rel=\"noreferrer noopener\">GeneArt\u2122 Gibson Assembly\u2122 HiFi Master Mix<\/a>.<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa84d8092489&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa84d8092489\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/Gibson-rxn-1024x372.png\" alt=\"Illustration of the Gibson Assembly process\" class=\"wp-image-4636\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u8bf7\u5c0f\u5fc3\u5730\u5c06\u60a8\u8bbe\u8ba1\u7684dna\u7247\u6bb5\u548c\u7ebf\u6027\u5316\u8f7d\u4f53\u6df7\u5408\">\u8bf7\u5c0f\u5fc3\u5730\u5c06\u60a8\u8bbe\u8ba1\u7684DNA\u7247\u6bb5\u548c\u7ebf\u6027\u5316\u8f7d\u4f53\u6df7\u5408<\/h4>\n\n\n\n<p>\u786e\u4fdd\u51c6\u786e\u8ba1\u7b97\u5e76\u6b63\u786e\u5206\u914d\u5355\u7ba1\u53cd\u5e94\u4e2d\u6bcf\u79cd\u7ec4\u5206\u7684\u7528\u91cf\u3002\u5173\u6ce8\u8fd9\u4e9b\u7ec6\u8282\u6709\u52a9\u4e8e\u5b9e\u73b0\u9ad8\u6548\u7684DNA\u7247\u6bb5\u7ec4\u88c5\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5305\u542b\u5bf9\u7167\u53cd\u5e94\">\u5305\u542b\u5bf9\u7167\u53cd\u5e94<\/h4>\n\n\n\n<p>\u5728\u5b9e\u9a8c\u4e2d\u59cb\u7ec8\u5305\u542b\u9633\u6027\u548c\u9634\u6027\u5bf9\u7167\u3002\u9633\u6027\u5bf9\u7167\u53ef\u786e\u8ba4\u7ec4\u88c5\u53cd\u5e94\u6761\u4ef6\u7684\u6709\u6548\u6027\uff0c\u800c\u9634\u6027\u5bf9\u7167\uff08\u7f3a\u5c11\u4e00\u4e2a\u6216\u591a\u4e2aDNA\u7247\u6bb5\uff09\u6709\u52a9\u4e8e\u8bc6\u522b\u6f5c\u5728\u6c61\u67d3\u6216\u975e\u7279\u5f02\u6027\u7ec4\u88c5\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-3-\u8f6c\u5316\u611f\u53d7\u6001\u7ec6\u80de\">3. \u8f6c\u5316\u611f\u53d7\u6001\u7ec6\u80de<\/h3>\n\n\n\n<p>\u4f7f\u7528\u9ad8\u6548\u611f\u53d7\u6001\u7ec6\u80de\uff0c\u4f8b\u5982&nbsp;<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cloning\/competent-cells-for-transformation\/competent-cells-strains\/top10-competent-cells.html\" target=\"_blank\" rel=\"noreferrer noopener\">One Shot\u2122 TOP10 Chemically Competent E. coli<\/a>, \u4ee5\u6700\u5927\u5316\u83b7\u5f97\u6210\u529f\u7684\u8f6c\u5316\u5b50\u6570\u91cf\u3002\u9002\u5f53\u7684\u5b75\u80b2\u65f6\u95f4\u548c\u6e29\u5ea6\u5bf9\u4e8e\u83b7\u5f97\u6700\u4f73\u7ed3\u679c\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa84d8092ada&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa84d8092ada\" class=\"aligncenter size-large wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/Cell-transformation-1024x480.png\" alt=\"Illustration of Transforming Competent Cells \" class=\"wp-image-4637\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><\/figure>\n<\/div>\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u5c06\u8f6c\u5316\u6df7\u5408\u7269\u6d82\u5e03\u5728\u5408\u9002\u7684\u5e73\u677f\u4e0a\">\u5c06\u8f6c\u5316\u6df7\u5408\u7269\u6d82\u5e03\u5728\u5408\u9002\u7684\u5e73\u677f\u4e0a<\/h4>\n\n\n\n<p>\u5c06\u8f6c\u5316\u540e\u7684\u7ec6\u80de\u6d82\u5e03\u5728\u9009\u62e9\u6027LB\u743c\u8102\u5e73\u677f\u4e0a\uff0c\u4ee5\u5206\u79bb\u5355\u4e2a\u83cc\u843d\u3002\u6b64\u6b65\u9aa4\u5bf9\u4e8e\u786e\u4fdd\u53ea\u6709\u542b\u6709\u6b63\u786e\u7ec4\u88c5DNA\u6784\u5efa\u4f53\u7684\u7ec6\u80de\u624d\u80fd\u751f\u957f\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-4-\u7b5b\u9009\u6784\u5efa\u4f53\u7684\u83cc\u843d\">4. 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data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/Screening-colonies-1024x605.png\" alt=\"Illustration of Screening Colonies for Constuct \" class=\"wp-image-4639\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 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data-wp-interactive=\"core\/image\" data-wp-key=\"6aa84d809389f\" class=\"aligncenter size-full wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/QA2.png\" alt=\"Effect of Assembly reaction time on yield (R&amp;D data)\" class=\"wp-image-4633\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><em><em>\u7ec4\u88c5\u53cd\u5e94\u65f6\u95f4\u5bf9\u4ea7\u91cf\u7684\u5f71\u54cd\uff08\u7814\u53d1\u6570\u636e\uff09<\/em><\/em><\/figcaption><\/figure>\n<\/div>\n\n\n<p>\u4e3a\u4e86\u8282\u7701\u65f6\u95f4\uff0c\u60a8\u53ef\u4ee5\u7f29\u77ed\u7ec4\u88c5\u65f6\u95f4\u3002\u8be5\u534f\u8bae\u65f6\u95f4\u9488\u5bf9\u4e0d\u540c\u7684\u7ec4\u88c5\u8fdb\u884c\u4e86\u4f18\u5316\uff0c\u786e\u4fdd\u4ea7\u91cf\u4e0d\u4f1a\u53d7\u5230\u663e\u8457\u5f71\u54cd\u3002\u8fd9\u79cd\u7b80\u5316\u7684\u65b9\u6cd5\u4e0d\u4ec5\u8282\u7701\u65f6\u95f4\uff0c\u8fd8\u80fd\u4fdd\u6301\u6709\u6548\u7684\u7ed3\u679c\uff0c\u4f7f\u60a8\u7684\u5de5\u4f5c\u6d41\u7a0b\u66f4\u52a0\u9ad8\u6548\u3002<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u4f7f\u7528\u5feb\u901f\u8f6c\u5316\u65b9\u6848\">\u4f7f\u7528\u5feb\u901f\u8f6c\u5316\u65b9\u6848<\/h4>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa84d8093c09&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa84d8093c09\" class=\"aligncenter size-full wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/05\/QA3.png\" alt=\"Comparison of Rapid DNA and Standard Transformation protocols (R&amp;D data)\" class=\"wp-image-4634\" \/><button\n\t\t\tclass=\"lightbox-trigger\"\n\t\t\ttype=\"button\"\n\t\t\taria-haspopup=\"dialog\"\n\t\t\taria-label=\"\u653e\u5927\"\n\t\t\tdata-wp-init=\"callbacks.initTriggerButton\"\n\t\t\tdata-wp-on--click=\"actions.showLightbox\"\n\t\t\tdata-wp-style--right=\"state.imageButtonRight\"\n\t\t\tdata-wp-style--top=\"state.imageButtonTop\"\n\t\t>\n\t\t\t<svg xmlns=\"http:\/\/www.w3.org\/2000\/svg\" width=\"12\" height=\"12\" fill=\"none\" viewBox=\"0 0 12 12\">\n\t\t\t\t<path fill=\"#fff\" d=\"M2 0a2 2 0 0 0-2 2v2h1.5V2a.5.5 0 0 1 .5-.5h2V0H2Zm2 10.5H2a.5.5 0 0 1-.5-.5V8H0v2a2 2 0 0 0 2 2h2v-1.5ZM8 12v-1.5h2a.5.5 0 0 0 .5-.5V8H12v2a2 2 0 0 1-2 2H8Zm2-12a2 2 0 0 1 2 2v2h-1.5V2a.5.5 0 0 0-.5-.5H8V0h2Z\" \/>\n\t\t\t<\/svg>\n\t\t<\/button><figcaption class=\"wp-element-caption\"><em><em>\u5feb\u901fDNA\u8f6c\u5316\u4e0e\u6807\u51c6\u8f6c\u5316\u65b9\u6848\u7684\u6bd4\u8f83\uff08\u7814\u53d1\u6570\u636e\uff09<\/em><\/em><\/figcaption><\/figure>\n<\/div>\n\n\n<p>\u5bf9\u4e8e\u5e0c\u671b\u52a0\u5feb\u8f6c\u5316\u8fc7\u7a0b\u7684\u7528\u6237\uff0c\u53ef\u4ee5\u8003\u8651\u4f7f\u7528\u5feb\u901f\u8f6c\u5316\u65b9\u6848\u3002\u8fd9\u79cd\u65b9\u6cd5\u5305\u62ec\u7f29\u77ed\u5b75\u80b2\u65f6\u95f4\uff0c\u5e76\u4f7f\u7528\u4e13\u4e3a\u5feb\u901f\u8f6c\u5316\u800c\u4f18\u5316\u7684\u8bd5\u5242\u3002\u53ea\u9700\u5c06\u611f\u53d7\u6001\u7ec6\u80de\u4e0eDNA\u6df7\u5408\uff0c\u7136\u540e\u76f4\u63a5\u6d82\u5e03\u5230\u9009\u62e9\u6027LB\u743c\u8102\u5e73\u677f\u4e0a\uff0c\u65e0\u9700\u70ed\u6fc0\u6216\u6062\u590d\u6b65\u9aa4\u3002<\/p>\n\n\n\n<p>\u8be5\u65b9\u6cd5\u5728\u4f7f\u7528\u6c28\u82c4\u9752\u9709\u7d20\u9009\u62e9\u5e73\u677f\u8fdb\u884c\u8f6c\u5316\u65f6\u5c24\u5176\u6709\u6548\u3002\u5feb\u901f\u65b9\u6848\u53ef\u663e\u8457\u51cf\u5c11\u6240\u9700\u65f6\u95f4\uff0c\u540c\u65f6\u8f6c\u5316\u6548\u7387\u4ec5\u6709\u5fae\u5c0f\u53d8\u5316\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-golden-gate\u7ec4\u88c5\u4e0egibson\u7ec4\u88c5-\u6709\u4ec0\u4e48\u533a\u522b-\u6211\u5e94\u8be5\u7528\u54ea\u4e00\u4e2a\u8fdb\u884c\u514b\u9686\">2. Golden Gate\u7ec4\u88c5\u4e0eGibson\u7ec4\u88c5\uff1a\u6709\u4ec0\u4e48\u533a\u522b\uff0c\u6211\u5e94\u8be5\u7528\u54ea\u4e00\u4e2a\u8fdb\u884c\u514b\u9686\uff1f<\/h3>\n\n\n\n<p>\u5728DNA\u514b\u9686\u65b9\u9762\uff0c\u9009\u62e9\u5408\u9002\u7684\u7ec4\u88c5\u65b9\u6cd5\u4f1a\u663e\u8457\u5f71\u54cd\u5b9e\u9a8c\u7684\u6210\u529f\u3002Golden Gate\u7ec4\u88c5\u548cGibson\u7ec4\u88c5\u662f\u4e24\u79cd\u5e38\u7528\u6280\u672f\uff0c\u5404\u81ea\u5177\u6709\u72ec\u7279\u4f18\u52bf\u3002<\/p>\n\n\n\n<p>Golden Gate\u7ec4\u88c5\u5229\u7528IIS\u578b\u9650\u5236\u6027\u5185\u5207\u9176\u6784\u5efa\u65e0\u7f1dDNA\u7247\u6bb5\uff0c\u56e0\u6b64\u5728\u91cd\u590d\u6027\u514b\u9686\u4efb\u52a1\u4e2d\u6548\u7387\u5f88\u9ad8\u3002\u800cGibson\u7ec4\u88c5\u5219\u901a\u8fc7\u6df7\u5408\u591a\u79cd\u9176\uff0c\u5728\u5355\u4e00\u6052\u6e29\u53cd\u5e94\u4e2d\u8fde\u63a5\u591a\u4e2aDNA\u7247\u6bb5\uff0c\u9002\u7528\u4e8e\u7ec4\u88c5\u66f4\u5927\u3001\u66f4\u590d\u6742\u7684\u7ed3\u6784\u3002<\/p>\n\n\n\n<p>\u5982\u679c\u4f60\u7684\u9879\u76ee\u9700\u8981\u9ad8\u7cbe\u5ea6\u7684\u6a21\u5757\u5316\u514b\u9686\uff0cGolden Gate \u65b9\u6cd5\u53ef\u80fd\u662f\u66f4\u4f18\u7684\u9009\u62e9\u3002\u7136\u800c\uff0c\u5982\u679c\u9700\u8981\u540c\u65f6\u7ec4\u88c5\u5927\u7247\u6bb5DNA\u6216\u591a\u4e2a\u63d2\u5165\u7247\u6bb5\uff0cGibson Assembly \u53ef\u80fd\u66f4\u4e3a\u5408\u9002\u3002\u4e86\u89e3\u514b\u9686\u9879\u76ee\u7684\u5177\u4f53\u9700\u6c42\u5c06\u6709\u52a9\u4e8e\u4f60\u51b3\u5b9a\u4f7f\u7528\u54ea\u79cd\u65b9\u6cd5\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-3-\u5982\u4f55\u8bbe\u8ba1gibson\u7ec4\u88c5\u7684\u5f15\u7269\">3. \u5982\u4f55\u8bbe\u8ba1Gibson\u7ec4\u88c5\u7684\u5f15\u7269\uff1f<\/h3>\n\n\n\n<p>\u4e3aGibson\u7ec4\u88c5\u8bbe\u8ba1\u5f15\u7269\u65f6\uff0c\u9700\u8981\u521b\u5efa\u5728\u5176\u672b\u7aef\u5177\u6709\u91cd\u53e0\u5e8f\u5217\u7684\u5f15\u7269\uff0c\u4ee5\u4fbf\u4e0e\u5f85\u8fde\u63a5\u7684DNA\u7247\u6bb5\u672b\u7aef\u5339\u914d\u3002\u4ee5\u4e0b\u662f\u5173\u952e\u6b65\u9aa4\uff1a<\/p>\n\n\n\n<ol start=\"1\" class=\"wp-block-list\">\n<li><strong>\u786e\u5b9a\u91cd\u53e0\u533a\u57df<\/strong>\uff1a\u786e\u5b9a\u6bcf\u4e2a\u7247\u6bb5\u8fde\u63a5\u6240\u9700\u768420-40\u4e2a\u78b1\u57fa\u7684\u91cd\u53e0\u5e8f\u5217\u3002<\/li>\n\n\n\n<li><strong>\u8bbe\u8ba1\u5f15\u7269\u5e8f\u5217<\/strong>\uff1a\u786e\u4fdd\u6bcf\u6761\u5f15\u7269\u76845\u2032\u7aef\u5305\u542b\u91cd\u53e0\u5e8f\u5217\uff0c\u800c3\u2032\u7aef\u5219\u4e3a\u4e0e\u76ee\u6807DNA\u4e92\u8865\u7684\u533a\u57df\u3002<\/li>\n\n\n\n<li><strong>\u68c0\u67e5\u7194\u89e3\u6e29\u5ea6<\/strong>\uff1a\u786e\u8ba4\u6240\u8bbe\u8ba1\u5f15\u7269\u7684\u7194\u89e3\u6e29\u5ea6\uff08Tm\uff09\u76f8\u5bb9\uff0c\u7406\u60f3\u60c5\u51b5\u4e0b\u5f7c\u6b64\u4e4b\u95f4\u7684\u5dee\u5f02\u5e94\u57282-3\u00b0C\u4ee5\u5185\u3002\u9009\u62e9\u7194\u89e3\u6e29\u5ea6\uff08Tm\uff09\u9ad8\u4e8e50\u00b0C\u7684\u540c\u6e90\u91cd\u53e0\u533a\u57df\u53ef\u4ee5\u63d0\u9ad8\u7ec4\u88c5\u6548\u7387\u3002<\/li>\n\n\n\n<li><strong>\u907f\u514d\u4e8c\u7ea7\u7ed3\u6784<\/strong>\uff1a\u4f7f\u7528\u8f6f\u4ef6\u5de5\u5177\u786e\u4fdd\u60a8\u7684\u5f15\u7269\u4e0d\u4f1a\u5f62\u6210\u53d1\u5939\u7ed3\u6784\u6216\u4e8c\u805a\u4f53\u3002\u9075\u5faa\u8fd9\u4e9b\u6b65\u9aa4\u5c06\u5e2e\u52a9\u60a8\u8bbe\u8ba1\u51fa\u9ad8\u6548\u7684\u5f15\u7269\uff0c\u4ee5\u786e\u4fddGibson\u7ec4\u88c5\u53cd\u5e94\u7684\u6210\u529f\u3002<\/li>\n<\/ol>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-4-dna\u7247\u6bb5\u8fdb\u884cgibson\u7ec4\u88c5\u65f6\u5408\u9002\u7684\u91cd\u53e0\u957f\u5ea6\u662f\u591a\u5c11\">4. DNA\u7247\u6bb5\u8fdb\u884cGibson\u7ec4\u88c5\u65f6\u5408\u9002\u7684\u91cd\u53e0\u957f\u5ea6\u662f\u591a\u5c11\uff1f<\/h3>\n\n\n\n<p>Gibson\u7ec4\u88c5\u7684\u5408\u9002\u91cd\u53e0\u957f\u5ea6\u901a\u5e38\u572820\u523040\u4e2a\u78b1\u57fa\u5bf9\u4e4b\u95f4\u3002\u8fd9\u4e00\u957f\u5ea6\u8db3\u4ee5\u786e\u4fdd\u7247\u6bb5\u4e4b\u95f4\u80fd\u591f\u7279\u5f02\u6027\u4e14\u7a33\u5b9a\u5730\u9000\u706b\u7ed3\u5408\u3002<\/p>\n\n\n\n<p>\u77ed\u4e8e20 bp\u7684\u91cd\u53e0\u53ef\u80fd\u65e0\u6cd5\u63d0\u4f9b\u8db3\u591f\u7684\u7279\u5f02\u6027\u548c\u7a33\u5b9a\u6027\uff0c\u4ece\u800c\u5bfc\u81f4\u7ec4\u88c5\u6548\u7387\u4f4e\u4e0b\uff1b\u800c\u957f\u4e8e40 bp\u7684\u91cd\u53e0\u53ef\u80fd\u4e0d\u4f1a\u663e\u8457\u63d0\u9ad8\u6548\u7387\uff0c\u53cd\u800c\u4f1a\u4f7f\u5f15\u7269\u8bbe\u8ba1\u66f4\u52a0\u590d\u6742\u3002\u56e0\u6b64\uff0c\u5728Gibson\u7ec4\u88c5\u8fc7\u7a0b\u4e2d\uff0c\u4fdd\u6301\u5728\u6b64\u8303\u56f4\u5185\u8bbe\u8ba1\u91cd\u53e0\u5e8f\u5217\u5bf9\u4e8e\u5b9e\u9a8c\u7684\u6210\u529f\u81f3\u5173\u91cd\u8981\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-gibson\u7b49\u6e29\u7ec4\u88c5-\u4e00\u79cd\u4fbf\u6377\u5feb\u901f\u7684\u66ff\u4ee3\u514b\u9686\u65b9\u6cd5\">Gibson\u7b49\u6e29\u7ec4\u88c5\uff1a\u4e00\u79cd\u4fbf\u6377\u5feb\u901f\u7684\u66ff\u4ee3\u514b\u9686\u65b9\u6cd5<\/h2>\n\n\n\n<p>Gibson\u7ec4\u88c5\u662f\u4e00\u79cd\u591a\u529f\u80fd\u4e14\u9ad8\u6548\u7684DNA\u7ec4\u88c5\u65b9\u6cd5\uff0c\u901a\u8fc7\u7cbe\u5fc3\u89c4\u5212\u548c\u6ce8\u91cd\u7ec6\u8282\uff0c\u60a8\u53ef\u4ee5\u638c\u63e1\u8be5\u6280\u672f\u3002\u901a\u8fc7\u9075\u5faa\u4e0a\u8ff0\u5173\u952e\u6280\u5de7\uff0c\u60a8\u53ef\u4ee5\u4f18\u5316\u5b9e\u9a8c\u65b9\u6848\uff0c\u5728\u514b\u9686\u5b9e\u9a8c\u4e2d\u83b7\u5f97\u53ef\u9760\u4e14\u9ad8\u8d28\u91cf\u7684\u7ed3\u679c\u3002<\/p>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cloning\/seamless-cloning-and-genetic-assembly\/gibson-assembly.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong> \u63a2\u7d22 GeneArt Gibson \u7ec4\u88c5\u514b\u9686\u6280\u672f<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u76f8\u5173\u7684gibson\u7ec4\u88c5\u8d44\u6e90\u4e0e\u6280\u672f\">\u76f8\u5173\u7684Gibson\u7ec4\u88c5\u8d44\u6e90\u4e0e\u6280\u672f<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u4ea7\u54c1: <\/strong><a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/A46624\" target=\"_blank\" rel=\"noreferrer noopener\">GeneArt Gibson \u7ec4\u88c5\u9ad8\u4fdd\u771f\u514b\u9686\u8bd5\u5242\u76d2<\/a><\/li>\n\n\n\n<li><strong>\u5b9e\u9a8c\u6d41\u7a0b:<\/strong> <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/Technical-Notes\/mutagenesis-geneart-tech-note.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">\u4f7f\u7528 GeneArt Gibson Assembly HiFi \u514b\u9686\u8bd5\u5242\u76d2\u8fdb\u884c\u5b9a\u70b9\u8bf1\u53d8<\/a><\/li>\n\n\n\n<li><strong>\u767d\u76ae\u4e66: <\/strong><a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/Application-Notes\/equiphi29-dna-amplification-kit-app-note.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">\u5229\u7528\u65e0\u7ec6\u80de Gibson \u7ec4\u88c5\u548c\u6eda\u73af\u6269\u589e\u5de5\u4f5c\u6d41\u7a0b\u5b9e\u73b0\u4ece DNA \u6784\u5efa\u5230\u86cb\u767d\u8d28\u8868\u8fbe\u4ec5\u9700\u4e00\u5929<\/a><\/li>\n\n\n\n<li><strong>\u5b66\u4e60\u9875\u9762: <\/strong><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/spotlight-articles\/dna-cloning-tips-build-clones-dna-fragments-gibson-assembly.html\" target=\"_blank\" rel=\"noreferrer noopener\">DNA \u514b\u9686\u6280\u5de7\u2014\u2014\u4f7f\u7528 DNA \u7247\u6bb5\u901a\u8fc7 Gibson \u7ec4\u88c5\u6784\u5efa\u514b\u9686<\/a><\/li>\n\n\n\n<li><strong>\u767d\u76ae\u4e66:<\/strong> <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/BID\/Reference-Materials\/gibson-assembly-hifi-homology-oligonucleotide-stitching-white-paper.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">\u7ed3\u5408\u540c\u6e90\u91cd\u7ec4\u4e0e\u6838\u82f7\u9178\u62fc\u63a5\u6280\u672f\u6784\u5efa\u5927\u578b\u8d28\u7c92<\/a><\/li>\n<\/ul>\n\n\n\n<p><\/p>\n\n\n\n<p>##<\/p>\n\n\n\n<p><em>\u00a9 2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise<\/em>&nbsp;<em>specified.<\/em> <em>SnapGene is a trademark of GSL Biotech LLC. Gibson Assembly is a trademark of Telesis Bio Inc.<\/em><\/p>\n\n\n\n<p><em>For Research Use Only. Not for use in diagnostic procedures.<\/em><\/p>\n\n\n<script type=\"text\/javascript\">\n\/* <![CDATA[ *\/\nvar gform;gform||(document.addEventListener(\"gform_main_scripts_loaded\",function(){gform.scriptsLoaded=!0}),document.addEventListener(\"gform\/theme\/scripts_loaded\",function(){gform.themeScriptsLoaded=!0}),window.addEventListener(\"DOMContentLoaded\",function(){gform.domLoaded=!0}),gform={domLoaded:!1,scriptsLoaded:!1,themeScriptsLoaded:!1,isFormEditor:()=>\"function\"==typeof InitializeEditor,callIfLoaded:function(o){return!(!gform.domLoaded||!gform.scriptsLoaded||!gform.themeScriptsLoaded&&!gform.isFormEditor()||(gform.isFormEditor()&&console.warn(\"The use of gform.initializeOnLoaded() is deprecated in the form editor context and will be removed in Gravity Forms 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