{"id":2217,"date":"2026-10-28T02:19:47","date_gmt":"2026-10-28T02:19:47","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=2217"},"modified":"2026-10-28T02:19:49","modified_gmt":"2026-10-28T02:19:49","slug":"multiplex-qpcr-optimization-6-plex","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/multiplex-qpcr-optimization-6-plex\/","title":{"rendered":"5\u91cd\u548c6\u91cd\u68c0\u6d4b\u7684\u591a\u91cdqPCR\u4f18\u5316"},"content":{"rendered":"\n<p class=\"wp-block-paragraph\">\u5f53\u591a\u91cd qPCR \u68c0\u6d4b\u4e2d\u5f15\u5165\u66f4\u591a\u9776\u6807\u540e\u5f00\u59cb\u51fa\u73b0\u6027\u80fd\u4e0b\u964d\u65f6\uff0c\u4f1a\u53d1\u751f\u4ec0\u4e48\uff1f\u4e00\u79cd\u5728\u5355\u91cd\u68c0\u6d4b\u4e2d\u8868\u73b0\u7a33\u5b9a\u4e14\u53ef\u91cd\u590d\u7684\u68c0\u6d4b\u65b9\u6cd5\uff0c\u5728\u6269\u5c55\u5230 5 \u91cd\u6216 6 \u91cd\u68c0\u6d4b\u65f6\uff0c\u53ef\u80fd\u4f1a\u51fa\u73b0 Ct \u503c\u504f\u79fb\u3001\u7075\u654f\u5ea6\u964d\u4f4e\u6216\u52a8\u6001\u8303\u56f4\u538b\u7f29\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u968f\u7740\u591a\u91cd\u6c34\u5e73\u7684\u63d0\u9ad8\uff0c\u5f15\u7269\u3001\u63a2\u9488\u3001\u62a5\u544a\u67d3\u6599\u548c\u805a\u5408\u9176\u4e4b\u95f4\u7684\u7ade\u4e89\u4f1a\u52a0\u5267\uff0c\u800c\u5fae\u5c0f\u7684\u4e0d\u5e73\u8861\u5728\u591a\u91cd\u6761\u4ef6\u4e0b\u4e5f\u53ef\u80fd\u88ab\u653e\u5927\u3002\u5982\u679c\u53cd\u5e94\u7ec4\u5206\u5e76\u975e\u4e13\u4e3a\u5728\u591a\u91cd\u6761\u4ef6\u4e0b\u534f\u540c\u5de5\u4f5c\u800c\u8bbe\u8ba1\uff0c\u9002\u5ea6\u7684 \u0394Ct \u53d8\u5316\u5c31\u53ef\u80fd\u5f71\u54cd\u5b9a\u91cf\u51c6\u786e\u6027\u3002\u8981\u6210\u529f\u5c06\u591a\u91cd qPCR \u6269\u5c55\u5230\u66f4\u9ad8\u6c34\u5e73\uff0c\u4ec5\u4ec5\u662f\u5728\u540c\u4e00\u53cd\u5e94\u4e2d\u52a0\u5165\u66f4\u591a\u68c0\u6d4b\u9776\u6807\u662f\u4e0d\u591f\u7684\uff1b\u8fd8\u9700\u8981\u5bf9\u63a2\u9488\u5316\u5b66\u3001\u67d3\u6599\u517c\u5bb9\u6027\u548c\u9884\u6df7\u6db2\u914d\u65b9\u8fdb\u884c\u534f\u540c\u4f18\u5316\u3002<\/p>\n\n\n\n<div class=\"wp-block-tf-blocks-intro-summary\">\n<h2 class=\"wp-block-heading\" id=\"h-\u60a8\u5c06\u5728\u672c\u6587\u4e2d\u4e86\u89e3\u5230\u7684\u5185\u5bb9\"><strong><strong>\u60a8\u5c06\u5728\u672c\u6587\u4e2d\u4e86\u89e3\u5230\u7684\u5185\u5bb9<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">T\u4e3a\u4e86\u5728\u4e0d\u727a\u7272\u7075\u654f\u5ea6\u7684\u60c5\u51b5\u4e0b\u6709\u628a\u63e1\u5730\u4ece 1-plex \u6269\u5c55\u5230 6-plex\uff0c\u7406\u89e3\u591a\u91cd\u5de5\u4f5c\u6d41\u7a0b\u901a\u5e38\u5728\u54ea\u4e9b\u73af\u8282\u51fa\u73b0\u6027\u80fd\u4e0b\u964d\u4ee5\u53ca\u5982\u4f55\u51cf\u8f7b\u8fd9\u4e9b\u5f71\u54cd\u975e\u5e38\u91cd\u8981\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u672c\u6587\u5c06\u6db5\u76d6\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u4e3a\u4ec0\u4e48\u5728\u591a\u91cd\u6269\u5c55\u8fc7\u7a0b\u4e2d\u4f1a\u53d1\u751f \u0394Ct \u504f\u79fb<\/li>\n\n\n\n<li>\u63a2\u9488\u5316\u5b66\u5982\u4f55\u5f71\u54cd\u4fe1\u53f7\u5f3a\u5ea6\u548c\u5149\u8c31\u5206\u79bb<\/li>\n\n\n\n<li>\u9884\u6df7\u6db2\u914d\u65b9\u5728\u7ef4\u6301\u6269\u589e\u6548\u7387\u548c\u52a8\u6001\u8303\u56f4\u65b9\u9762\u7684\u4f5c\u7528<\/li>\n\n\n\n<li>\u5728\u66f4\u9ad8\u91cd\u6570\u53cd\u5e94\u4e2d\u4fdd\u6301\u4f4e\u62f7\u8d1d\u68c0\u6d4b\u80fd\u529b\u65f6\u9700\u8981\u8003\u8651\u7684\u56e0\u7d20<\/li>\n\n\n\n<li>\u8bc4\u4f30\u548c\u4f18\u5316 5-plex \u4e0e 6-plex \u68c0\u6d4b\u7684\u5b9e\u7528\u6b65\u9aa4<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e86\u89e3\u66f4\u591a\u4fe1\u606f\uff0c\u5305\u62ec\u652f\u6301\u53ef\u9760\u9ad8\u9636\u591a\u91cd qPCR \u5f00\u53d1\u7684\u5316\u5b66\u539f\u7406\u548c\u5de5\u4f5c\u6d41\u7a0b\u6ce8\u610f\u4e8b\u9879\u3002<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-tf-blocks-definition-block\">\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u662f\u591a\u91cd-qpcr\"><strong><strong>\u4ec0\u4e48\u662f\u591a\u91cd qPCR\uff1f<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u591a\u91cd qPCR \u662f\u6307\u5728\u5355\u6b21\u53cd\u5e94\u4e2d\u6269\u589e\u4e00\u4e2a\u4ee5\u4e0a\u7684\u9776\u6807\uff0c\u5e76\u4f7f\u7528\u4e0d\u540c\u7684\u62a5\u544a\u67d3\u6599\u6765\u533a\u5206\u5404\u4e2a\u9776\u6807\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u5178\u578b\u5de5\u4f5c\u6d41\u7a0b\u4e2d\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u6bcf\u4e2a\u68c0\u6d4b\/\u9776\u6807\u5bf9\u90fd\u6807\u8bb0\u6709\u4e0d\u540c\u7684\u62a5\u544a\u67d3\u6599<\/li>\n\n\n\n<li>\u6240\u6709\u68c0\u6d4b\u4e0e\u5355\u4e00\u4e3b\u6df7\u5408\u6db2\u548c\u6a21\u677f\u7ec4\u5408\u5728\u4e00\u8d77<\/li>\n\n\n\n<li>\u6269\u589e\u8fc7\u7a0b\u901a\u8fc7\u72ec\u7acb\u7684\u5149\u5b66\u901a\u9053\u8fdb\u884c\u68c0\u6d4b<\/li>\n<\/ul>\n<\/div>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/workflow-multiplexing-1.png\" alt=\"Diagram showing four qPCR targets combined into a single reaction with master mix and detected across multiple channels.\" class=\"wp-image-19607\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\">\u6839\u636e\u68c0\u6d4b\u8bbe\u8ba1\uff0c\u591a\u91cd\u5316\u4e5f\u53ef\u4ee5\u662f\u5728\u5355\u4e00\u901a\u9053\u4e2d\u6269\u589e\u591a\u4e2a\u9776\u6807\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"aligncenter size-full\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/workflow-multiplexing-2.png\" alt=\"Diagram showing grouped qPCR targets combined into fewer assays within a multiplex reaction workflow.\" class=\"wp-image-19608\" \/><\/figure>\n<\/div>\n\n\n<p class=\"wp-block-paragraph\">W\u5728\u8bbe\u8ba1\u5f97\u5f53\u7684\u60c5\u51b5\u4e0b\uff0c\u591a\u91cd qPCR \u53ef\u5b9e\u73b0\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u51cf\u5c11\u8bd5\u5242\u548c\u6837\u672c\u7528\u91cf<\/li>\n\n\n\n<li>\u63d0\u9ad8\u901a\u91cf<\/li>\n\n\n\n<li>\u66f4\u77ed\u7684\u5de5\u4f5c\u6d41\u7a0b\u65f6\u95f4<\/li>\n\n\n\n<li>\u5728\u540c\u4e00\u53cd\u5e94\u4e2d\u6bd4\u8f83\u76ee\u6807\u65f6\u7684\u7cbe\u786e\u6027<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u7136\u800c\uff0c\u8fd9\u4e9b\u4f18\u52bf\u5728\u5f88\u5927\u7a0b\u5ea6\u4e0a\u53d6\u51b3\u4e8e\u68c0\u6d4b\u8bbe\u8ba1\u548c\u53cd\u5e94\u4f18\u5316\u3002\u4e86\u89e3\u4ec0\u4e48\u662f\u591a\u91cd qPCR \u53ea\u662f\u8d77\u70b9\u3002\u5728\u66f4\u9ad8\u91cd\u6570\u6c34\u5e73\u4e0b\u7684\u6027\u80fd\u53d6\u51b3\u4e8e\u68c0\u6d4b\u5982\u4f55\u8bbe\u8ba1\u4ee5\u53ca\u53cd\u5e94\u7ec4\u5206\u5982\u4f55\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u591a\u91cd\u68c0\u6d4b\u8bbe\u8ba1\u7684\u4e00\u822c\u6ce8\u610f\u4e8b\u9879\"><strong><strong>\u591a\u91cd\u68c0\u6d4b\u8bbe\u8ba1\u7684\u4e00\u822c\u6ce8\u610f\u4e8b\u9879<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">Successf\u6210\u529f\u7684\u591a\u91cd qPCR \u5de5\u4f5c\u6d41\u7a0b\u9700\u8981\u5728\u591a\u4e2a\u53d8\u91cf\u4e4b\u95f4\u5b9e\u73b0\u534f\u8c03\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5e94\u7528\u548c\u6837\u672c\u7c7b\u578b<\/li>\n\n\n\n<li>\u5f15\u7269\u548c\u63a2\u9488\u8bbe\u8ba1<\/li>\n\n\n\n<li>\u9884\u6df7\u6db2\u517c\u5bb9\u6027<\/li>\n\n\n\n<li>\u70ed\u5faa\u73af\u6761\u4ef6<\/li>\n\n\n\n<li>\u4eea\u5668\u548c\u67d3\u6599\u517c\u5bb9\u6027<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u5177\u4f53\u7684\u68c0\u6d4b\u8bbe\u8ba1\u8003\u8651\u56e0\u7d20\u5305\u62ec\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5c3d\u91cf\u51cf\u5c11\u975e\u7279\u5f02\u6027\u6269\u589e<\/li>\n\n\n\n<li>\u9009\u62e9\u53ef\u964d\u4f4e\u80cc\u666f\u4fe1\u53f7\u548c\u4e32\u6270\u7684\u67d3\u6599\/\u6dec\u706d\u57fa\u7ec4\u5408<\/li>\n\n\n\n<li>\u4f18\u5316\u6240\u6709\u9776\u6807\u7684\u9000\u706b\u6e29\u5ea6\u548c\u5ef6\u4f38\u65f6\u95f4<\/li>\n\n\n\n<li>\u9650\u5236\u68c0\u6d4b\u4e4b\u95f4\u7684\u7ade\u4e89\u6216\u6291\u5236\u4f5c\u7528<\/li>\n\n\n\n<li>\u8003\u8651\u4e0d\u540c\u9776\u6807\u4e4b\u95f4\u7684\u8868\u8fbe\u8303\u56f4\u5dee\u5f02<\/li>\n\n\n\n<li>\u4f18\u5316\u5f15\u7269\u548c\u63a2\u9488\u6d53\u5ea6\uff0c\u4ee5\u5e73\u8861\u6269\u589e\u6548\u7387\u5e76\u51cf\u5c11\u805a\u5408\u9176\u9971\u548c<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u968f\u7740\u591a\u91cd\u68c0\u6d4b\u6c34\u5e73\u7684\u63d0\u9ad8\uff0c\u8fd9\u4e9b\u53c2\u6570\u4f1a\u53d8\u5f97\u8d8a\u6765\u8d8a\u5173\u952e\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u63a2\u9488\u5316\u5b66\u5982\u4f55\u5f71\u54cd\u591a\u91cd\u68c0\u6d4b\u6027\u80fd\"><strong><strong>\u63a2\u9488\u5316\u5b66\u5982\u4f55\u5f71\u54cd\u591a\u91cd\u68c0\u6d4b\u6027\u80fd<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u63a2\u9488\u5316\u5b66\u4f1a\u76f4\u63a5\u5f71\u54cd\u4fe1\u53f7\u6e05\u6670\u5ea6\u3001\u80cc\u666f\u6291\u5236\u3001\u52a8\u6001\u8303\u56f4\u548c\u6269\u5c55\u6027\u80fd\u3002<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/oligonucleotides-primers-probes-genes\/applied-biosystems-custom-primers-probes.html\"><u>Applied Biosystems\u2122 QSY\u2122 \u63a2\u9488\u4ea7\u54c1\u7ec4\u5408\u914d\u5408 TaqMan\u2122 QSY2\u2122 \u63a2\u9488<\/u><\/a>\u652f\u6301\u66f4\u9ad8\u9636\u7684\u591a\u91cd\u68c0\u6d4b\u3002<br><br>\u4e3b\u8981\u7279\u6027\u5305\u62ec\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u66f4\u9ad8\u7684\u5206\u6790\u7075\u654f\u5ea6<\/li>\n\n\n\n<li>\u66f4\u5bbd\u7684\u52a8\u6001\u8303\u56f4<\/li>\n\n\n\n<li>\u4ece 1 \u91cd\u68c0\u6d4b\u6269\u5c55\u5230 6 \u91cd\u68c0\u6d4b\u65f6\u4ecd\u80fd\u4fdd\u6301\u6027\u80fd<\/li>\n\n\n\n<li>\u4e0e\u67d0\u4e9b\u66ff\u4ee3\u63a2\u9488\u76f8\u6bd4\uff0c1 \u91cd\u548c 6 \u91cd\u53cd\u5e94\u4e4b\u95f4\u7684 \u0394Ct \u5dee\u5f02\u66f4\u5c0f<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">QSY2 \u63a2\u9488\u652f\u6301\u4f7f\u7528 Cyanine 5 \u548c Cyanine 5.5 \u62a5\u544a\u67d3\u6599\u8fdb\u884c\u9ad8\u901a\u91cf 5 \u91cd\u548c 6 \u91cd\u53cd\u5e94\uff0c\u6709\u52a9\u4e8e\u5728\u4fdd\u6301\u4fe1\u53f7\u5206\u79bb\u7684\u540c\u65f6\u6269\u5c55\u591a\u91cd\u68c0\u6d4b\u9762\u677f\u5bb9\u91cf\u3002\u5728 MGB \u548c QSY \u6dec\u706d\u57fa\u56e2\u4e4b\u95f4\u5207\u6362\u65f6\uff0c\u5fc5\u987b\u91cd\u65b0\u8bbe\u8ba1\u63a2\u9488\uff0c\u8fd9\u8fdb\u4e00\u6b65\u8bf4\u660e\u67d3\u6599-\u6dec\u706d\u57fa\u56e2\u914d\u5bf9\u4e0d\u80fd\u4e92\u6362\u3002<br><br>\u5bf9\u4e8e\u5b9a\u5236\u591a\u91cd\u68c0\u6d4b\u9762\u677f\u5f00\u53d1\uff0c<a href=\"https:\/\/www.thermofisher.com\/tools\/oligos\/custom-primer-probe\"><u>Applied Biosystems\u2122 \u5b9a\u5236\u5f15\u7269\u548c\u63a2\u9488<\/u><\/a>\u63d0\u4f9b\u53ef\u914d\u7f6e\u7684\u67d3\u6599\u548c\u6dec\u706d\u57fa\u56e2\u9009\u9879\uff0c\u5728\u5355\u6b21\u53cd\u5e94\u4e2d\u6700\u591a\u652f\u6301\u516d\u4e2a\u9776\u6807\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e3a\u4ec0\u4e48-\u03b4ct-\u4fdd\u6301\u6027\u5728-6-\u91cd\u53cd\u5e94\u4e2d\u5f88\u91cd\u8981\"><strong><strong>\u4e3a\u4ec0\u4e48 \u0394Ct \u4fdd\u6301\u6027\u5728 6 \u91cd\u53cd\u5e94\u4e2d\u5f88\u91cd\u8981<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u4e00\u4e2a\u5173\u952e\u6027\u80fd\u6307\u6807\u662f\u5728\u4ece\u5355\u91cd\u53cd\u5e94\u6269\u5c55\u5230\u591a\u91cd\u53cd\u5e94\u65f6\u7684 \u0394Ct \u4fdd\u6301\u6027\u3002\u4e0e\u6240\u9009\u7684\u5176\u4ed6\u66ff\u4ee3\u63a2\u9488\u5316\u5b66\u4f53\u7cfb\u76f8\u6bd4\uff0cTaqMan QSY2 \u63a2\u9488\u5728\u5185\u90e8\u6027\u80fd\u8bc4\u4f30\u4e2d\u663e\u793a\u51fa 1 \u91cd\u4e0e 6 \u91cd\u53cd\u5e94\u4e4b\u95f4\u66f4\u5c0f\u7684 \u0394Ct \u5dee\u5f02\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u6269\u5c55\u8fc7\u7a0b\u4e2d\u4fdd\u6301\u8f83\u5c0f\u7684 \u0394Ct \u6709\u52a9\u4e8e\u7ef4\u6301\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5b9a\u91cf\u7f6e\u4fe1\u5ea6<\/li>\n\n\n\n<li>\u4f4e\u62f7\u8d1d\u7075\u654f\u5ea6<\/li>\n\n\n\n<li>\u52a8\u6001\u8303\u56f4\u7a33\u5b9a\u6027<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u6269\u5c55\u8fc7\u7a0b\u4e2d\u7684 \u0394Ct \u6f02\u79fb\u901a\u5e38\u53cd\u6620\u7684\u662f\u53cd\u5e94\u5931\u8861\uff0c\u800c\u4e0d\u53ea\u662f\u5f15\u7269\u8bbe\u8ba1\u672c\u8eab\u7684\u95ee\u9898\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e3b\u6df7\u5408\u6db2\u914d\u65b9\u5728\u591a\u91cd\u7a33\u5b9a\u6027\u4e2d\u7684\u4f5c\u7528\"><strong><strong>\u4e3b\u6df7\u5408\u6db2\u914d\u65b9\u5728\u591a\u91cd\u7a33\u5b9a\u6027\u4e2d\u7684\u4f5c\u7528<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u591a\u91cd\u53cd\u5e94\u7684\u6210\u529f\u5e76\u4e0d\u53ea\u53d6\u51b3\u4e8e\u68c0\u6d4b\u8bbe\u8ba1\u3002\u4e3b\u6df7\u5408\u6db2\u914d\u65b9\u662f\u5728\u591a\u91cd\u8d1f\u8f7d\u6761\u4ef6\u4e0b\u4fdd\u6301\u6027\u80fd\u7684\u4e3b\u8981\u56e0\u7d20\u3002Applied Biosystems\u2122 TaqPath\u2122 \u4ea7\u54c1\u7ec4\u5408\u5305\u542b\u7ecf\u9a8c\u8bc1\u9002\u7528\u4e8e\u591a\u91cd\u68c0\u6d4b\u5e94\u7528\u7684\u4e3b\u6df7\u5408\u6db2\uff0c\u5305\u62ec TaqPath\u2122 DuraPlex\u2122 1-Step RT-qPCR Master Mix<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58666\"><u>\u542b Rox<\/u><\/a>\u548c<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58669\"><u>\u4e0d\u542b Rox<\/u><\/a>\u4e13\u4e3a\u66f4\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b\u800c\u8bbe\u8ba1\u5e76\u4f18\u5316\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u5728\u590d\u6742\u6837\u672c\u57fa\u8d28\u4e2d\u4fdd\u6301\u6027\u80fd\"><strong><strong>\u5728\u590d\u6742\u6837\u672c\u57fa\u8d28\u4e2d\u4fdd\u6301\u6027\u80fd<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u591a\u91cd qPCR \u7684\u6027\u80fd\u5fc5\u987b\u5728\u591a\u79cd\u4e0d\u540c\u4e14\u6709\u65f6\u5177\u6709\u6291\u5236\u6027\u7684\u6837\u672c\u7c7b\u578b\u4e2d\u5f97\u4ee5\u4fdd\u6301\uff0c\u5305\u62ec\u8840\u6db2\u6765\u6e90\u57fa\u8d28\u3001\u75c5\u6bd2\u8f6c\u8fd0\u57f9\u517b\u57fa\u4ee5\u53ca\u751f\u7269\u5b66\u4e0a\u590d\u6742\u7684\u6837\u672c\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u7684\u5173\u952e\u8003\u8651\u56e0\u7d20\u5305\u62ec\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u5bf9\u4e34\u5e8a\u7814\u7a76\u6216\u751f\u7269\u6837\u672c\u4e2d\u5e38\u89c1\u6291\u5236\u5242\u5177\u6709\u9ad8\u8010\u53d7\u6027<\/li>\n\n\n\n<li>\u5728\u4e0d\u540c\u6a21\u677f\u8f93\u5165\u6761\u4ef6\u4e0b\u4fdd\u6301\u7a33\u5b9a\u6269\u589e<\/li>\n\n\n\n<li>\u517c\u5bb9\u81ea\u52a8\u5316\u6db2\u4f53\u5904\u7406\u7cfb\u7edf<\/li>\n\n\n\n<li>\u7ec4\u88c5\u540e\u53cd\u5e94\u4f53\u7cfb\u5728\u9ad8\u901a\u91cf\u5de5\u4f5c\u6d41\u7a0b\u4e2d\u7684\u53f0\u5f0f\u7a33\u5b9a\u6027<\/li>\n\n\n\n<li>\u7a33\u5b9a\u4e00\u81f4\u7684\u6279\u6b21\u95f4\u6027\u80fd\uff0c\u4ee5\u652f\u6301\u7eb5\u5411\u7814\u7a76<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u9488\u5bf9-6-\u4e2a\u9776\u6807\u4f18\u5316\u591a\u91cd-qpcr-\u7684\u6700\u4f73\u65b9\u6cd5\u662f\u4ec0\u4e48\"><strong><strong>\u9488\u5bf9 6 \u4e2a\u9776\u6807\u4f18\u5316\u591a\u91cd qPCR \u7684\u6700\u4f73\u65b9\u6cd5\u662f\u4ec0\u4e48\uff1f<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5728\u7406\u89e3\u5316\u5b66\u539f\u7406\u548c\u53cd\u5e94\u52a8\u529b\u5b66\u4e4b\u540e\uff0c\u4f18\u5316\u5de5\u4f5c\u5e94\u4f18\u5148\u5173\u6ce8\u5206\u6790\u91cd\u73b0\u6027\u3001\u4f4e\u62f7\u8d1d\u7075\u654f\u5ea6\u4ee5\u53ca\u5728\u4e0d\u540c\u6837\u672c\u7c7b\u578b\u4e2d\u7684\u7a33\u5065\u6027\u3002\u6709\u6548\u7684\u591a\u91cd qPCR \u4f18\u5316\u901a\u5e38\u5305\u62ec\uff1a<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5efa\u7acb\u5355\u91cd\u68c0\u6d4b\u7684\u57fa\u7ebf\u6269\u589e\u6548\u7387<\/li>\n\n\n\n<li>\u6bd4\u8f83\u5355\u91cd\u4e0e\u591a\u91cd\u683c\u5f0f\u4e4b\u95f4\u7684 \u0394Ct<\/li>\n\n\n\n<li>\u786e\u8ba4\u659c\u7387\u4e00\u81f4\u6027\u548c\u52a8\u6001\u8303\u56f4\u7684\u4fdd\u6301<\/li>\n\n\n\n<li>\u5728\u76f8\u5173\u8f93\u5165\u8303\u56f4\u5185\u8bc4\u4f30\u5206\u6790\u6027\u80fd<\/li>\n\n\n\n<li>\u4f7f\u7528\u5177\u6709\u4ee3\u8868\u6027\u7684\u6837\u672c\u57fa\u8d28\u8bc4\u4f30\u6291\u5236\u5242\u8010\u53d7\u6027<\/li>\n\n\n\n<li>\u786e\u8ba4\u4e0d\u540c\u8bd5\u5242\u6279\u6b21\u4e4b\u95f4\u7684\u91cd\u73b0\u6027<\/li>\n<\/ol>\n\n\n\n<p class=\"wp-block-paragraph\">\u6210\u529f\u5b9e\u73b0 6 \u91cd\u68c0\u6d4b\u6027\u80fd\u53d6\u51b3\u4e8e\u4e24\u4e2a\u4e3b\u8981\u56e0\u7d20\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u68c0\u6d4b\u8bbe\u8ba1\uff08\u5f15\u7269\/\u63a2\u9488\u5316\u5b66\u3001\u67d3\u6599-\u6dec\u706d\u5242\u914d\u5bf9\uff09<\/li>\n\n\n\n<li>\u9488\u5bf9\u591a\u91cd\u68c0\u6d4b\u4f18\u5316\u7684\u9884\u6df7\u6db2\u914d\u65b9<\/li>\n<\/ul>\n\n\n\n<p class=\"wp-block-paragraph\">\u63a2\u9488\u5316\u5b66\u6280\u672f\uff0c\u4f8b\u5982<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4482777\"><u>TaqMan QSY \u548c QSY2 \u63a2\u9488<\/u><\/a>\u7ecf\u8fc7\u4e13\u95e8\u8bbe\u8ba1\uff0c\u53ef\u652f\u6301\u66f4\u9ad8\u9636\u7684\u591a\u91cd\u68c0\u6d4b\uff0c\u5e76\u5728\u6269\u5c55\u89c4\u6a21\u65f6\u66f4\u597d\u5730\u4fdd\u6301\u6027\u80fd\uff1b\u800c\u9884\u6df7\u6db2\u5982<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58669\"><u>TaqPath DuraPlex<\/u><\/a>\u5219\u7ecf\u8fc7\u914d\u65b9\u4f18\u5316\uff0c\u53ef\u652f\u6301\u540c\u65f6\u8fdb\u884c\u591a\u9776\u6807\u6269\u589e\uff0c\u5e76\u5177\u6709\u5bbd\u5e7f\u7684\u52a8\u6001\u8303\u56f4\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u8fd9\u79cd\u7ec4\u5408\u6709\u52a9\u4e8e\u5728\u5c06\u68c0\u6d4b\u9762\u677f\u6269\u5c55\u7528\u4e8e\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u3001\u75c5\u6bd2\u68c0\u6d4b\u6216\u751f\u7269\u6807\u5fd7\u7269\u8bc4\u4f30\u65f6\uff0c\u4fdd\u6301\u5206\u6790\u7ed3\u679c\u7684\u53ef\u9760\u6027\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u51c6\u5907\u597d\u4f18\u5316\u591a\u91cd-qpcr-\u4e86\u5417\"><strong><strong>\u51c6\u5907\u597d\u4f18\u5316\u591a\u91cd qPCR \u4e86\u5417\uff1f<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\">\u5982\u9700\u4e86\u89e3\u4f18\u5316\u591a\u91cd\u53cd\u5e94\u7684\u5b9e\u7528\u5206\u6b65\u65b9\u6cd5\uff0c\u5305\u62ec\u68c0\u6d4b\u5e73\u8861\u548c\u89c4\u6a21\u653e\u5927\u8bc4\u4f30\u65b9\u9762\u7684\u6307\u5bfc\uff0c\u8bf7\u9605\u8bfb<a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/manuals\/taqman_optimization_man.pdf\"><u>TaqMan\u2122 \u591a\u91cd PCR \u4f18\u5316\u6307\u5357\u3002<\/u><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/documents.thermofisher.com\/TFS-Assets\/LSG\/manuals\/taqman_optimization_man.pdf\"><u>\u4e0b\u8f7d TaqMan \u591a\u91cd PCR \u4f18\u5316\u6307\u5357\uff0c\u4ee5\u652f\u6301\u68c0\u6d4b\u5f00\u53d1\u548c\u89c4\u6a21\u653e\u5927\u3002<\/u><\/a><\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5982\u9700\u4e86\u89e3\u672c\u6587\u8ba8\u8bba\u7684\u66f4\u591a\u6570\u636e\u548c\u68c0\u6d4b\u8bbe\u8ba1\u89c1\u89e3\uff0c\u8bf7\u89c2\u770b<a href=\"https:\/\/players.brightcove.net\/665001591001\/default_default\/index.html?videoId=6369340452112\"><u>\u7cbe\u901a\u591a\u91cd\u68c0\u6d4b\u7f51\u7edc\u7814\u8ba8\u4f1a<\/u><\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u63a2\u7d22\u4e13\u4e3a\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b\u800c\u8bbe\u8ba1\u7684-qpcr-\u591a\u91cd\u5c31\u7eea\u578b\u68c0\u6d4b\u548c\u9884\u6df7\u6db2\"><strong><strong>\u63a2\u7d22\u4e13\u4e3a\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b\u800c\u8bbe\u8ba1\u7684 qPCR \u591a\u91cd\u5c31\u7eea\u578b\u68c0\u6d4b\u548c\u9884\u6df7\u6db2\uff1a<\/strong><\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/oligonucleotides-primers-probes-genes\/applied-biosystems-custom-primers-probes.html\"><u>\u6d4f\u89c8 TaqMan \u591a\u91cd\u63a2\u9488\u548c qPCR \u5f15\u7269<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4316034\"><u>\u9009\u8d2d TaqM<\/u><\/a>&nbsp;<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4316034\"><u>\u9002\u7528\u4e8e 5 \u91cd\u591a\u91cd\u68c0\u6d4b\u7684 an MGB \u63a2\u9488<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/assay-design.html\"><u>\u8bbe\u8ba1\u5b9a\u5236\u591a\u91cd\u68c0\u6d4b<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58666\"><u>\u9009\u8d2d TaqPath DuraPlex \u4e00\u6b65\u6cd5 RT-qPCR \u9884\u6df7\u6db2<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/assay-support.html\"><u>\u54a8\u8be2\u591a\u91cd\u68c0\u6d4b\u4e13\u5bb6<\/u><\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6784\u5efa\u53ef\u9760\u7684-6-\u91cd\u68c0\u6d4b\u5de5\u4f5c\u6d41\u7a0b\u91c7\u7528\u4e13\u4e3a\u9ad8\u9636-qpcr-\u591a\u91cd\u68c0\u6d4b\u6027\u80fd\u800c\u8bbe\u8ba1\u7684\u63a2\u9488\u548c\u9884\u6df7\u6db2\"><strong>\u6784\u5efa\u53ef\u9760\u7684 6 \u91cd\u68c0\u6d4b\u5de5\u4f5c\u6d41\u7a0b\u91c7\u7528\u4e13\u4e3a\u9ad8\u9636 qPCR \u591a\u91cd\u68c0\u6d4b\u6027\u80fd\u800c\u8bbe\u8ba1\u7684\u63a2\u9488\u548c\u9884\u6df7\u6db2\u3002<\/strong><\/h2>\n\n\n\n<p class=\"wp-block-paragraph\"><a href=\"https:\/\/www.thermofisher.com\/taqman\/gene-expression\/assay\/query?keyword=*\"><u>TaqMan \u9884\u8bbe\u8ba1\u57fa\u56e0\u8868\u8fbe\u5206\u6790\u68c0\u6d4b<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.com\/order\/custom-genomic-products\/tools\/cadt\/\"><u>\u5b9a\u5236 TaqMan \u68c0\u6d4b<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4482777\"><u>TaqMan QSY \u548c QSY2 \u63a2\u9488<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58666\"><u>TaqPath DuraPlex \u4e00\u6b65\u6cd5 RT-qPCR \u9884\u6df7\u6db2<\/u><\/a><br><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/quantstudio-systems.html\"><u>QuantStudio \u5b9e\u65f6 PCR \u7cfb\u7edf<\/u><\/a><\/p>\n\n\n<style>.kt-accordion-id2217_577810-76 .kt-accordion-inner-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:10px;}.kt-accordion-id2217_577810-76 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;background:#ffffff;padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);}.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;background:#ffffff;color:#444444;padding-top:14px;padding-right:16px;padding-bottom:14px;padding-left:16px;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_577810-76 .kt-blocks-accordion-header:focus-visible{color:#444444;background:#ffffff;border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion--visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger, body:not(.hide-focus-outline) .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id2217_577810-76 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{color:#444444;background:#ffffff;border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_577810-76:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}@media all and (max-width: 1024px){.kt-accordion-id2217_577810-76 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}}@media all and (max-width: 1024px){.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}}@media all and (max-width: 1024px){.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_577810-76 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}}@media all and (max-width: 1024px){.kt-accordion-id2217_577810-76 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}}@media all and (max-width: 767px){.kt-accordion-id2217_577810-76 .kt-accordion-inner-wrap{display:block;}.kt-accordion-id2217_577810-76 .kt-accordion-inner-wrap .kt-accordion-pane:not(:first-child){margin-top:10px;}.kt-accordion-id2217_577810-76 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_577810-76 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}.kt-accordion-id2217_577810-76 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_577810-76 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}}<\/style>\n<div class=\"wp-block-kadence-accordion alignnone\"><div class=\"kt-accordion-wrap kt-accordion-id2217_577810-76 kt-accordion-has-2-panes kt-active-pane-0 kt-accordion-block kt-pane-header-alignment-left kt-accodion-icon-style-arrow kt-accodion-icon-side-right\" style=\"max-width:none\"><div class=\"kt-accordion-inner-wrap\" data-allow-multiple-open=\"false\" data-start-open=\"0\">\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-1 kt-pane2217_663d73-25\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\">\u591a\u91cd qPCR \u5e38\u89c1\u95ee\u9898\u89e3\u7b54<\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\"><style>.kt-accordion-id2217_3240f9-9f .kt-accordion-inner-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:10px;}.kt-accordion-id2217_3240f9-9f .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;background:#ffffff;padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);}.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;background:#ffffff;color:#444444;padding-top:14px;padding-right:16px;padding-bottom:14px;padding-left:16px;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f .kt-blocks-accordion-header:focus-visible{color:#444444;background:#ffffff;border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion--visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger, body:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id2217_3240f9-9f .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{color:#444444;background:#ffffff;border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_3240f9-9f:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}@media all and (max-width: 1024px){.kt-accordion-id2217_3240f9-9f .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}}@media all and (max-width: 1024px){.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}}@media all and (max-width: 1024px){.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}}@media all and (max-width: 1024px){.kt-accordion-id2217_3240f9-9f .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}}@media all and (max-width: 767px){.kt-accordion-id2217_3240f9-9f .kt-accordion-inner-wrap{display:block;}.kt-accordion-id2217_3240f9-9f .kt-accordion-inner-wrap .kt-accordion-pane:not(:first-child){margin-top:10px;}.kt-accordion-id2217_3240f9-9f .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_3240f9-9f .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}.kt-accordion-id2217_3240f9-9f .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_3240f9-9f > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}}<\/style>\n<div class=\"wp-block-kadence-accordion alignnone\"><div class=\"kt-accordion-wrap kt-accordion-id2217_3240f9-9f kt-accordion-has-2-panes kt-active-pane-0 kt-accordion-block kt-pane-header-alignment-left kt-accodion-icon-style-arrow kt-accodion-icon-side-right\" style=\"max-width:none\"><div class=\"kt-accordion-inner-wrap\" data-allow-multiple-open=\"false\" data-start-open=\"0\">\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-1 kt-pane2217_9b75d5-03\"><div class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong><strong>\u9ad8\u9636\u591a\u91cd qPCR \u4e2d\u6027\u80fd\u4e0b\u964d\u7684\u539f\u56e0\u662f\u4ec0\u4e48\uff1f<\/strong><\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/div><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p class=\"wp-block-paragraph\">\u9ad8\u9636\u591a\u91cd qPCR \u4e2d\u7684\u6027\u80fd\u4e0b\u964d\u53ef\u80fd\u6e90\u4e8e\u5f15\u7269\u3001\u63a2\u9488\u3001\u62a5\u544a\u67d3\u6599\u548c\u805a\u5408\u9176\u4e4b\u95f4\u7684\u7ade\u4e89\uff0c\u4ece\u800c\u5bfc\u81f4 Ct \u503c\u504f\u79fb\u3001\u6548\u7387\u964d\u4f4e\u4ee5\u53ca\u52a8\u6001\u8303\u56f4\u538b\u7f29\u3002<\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-2 kt-pane2217_8aa367-68\"><div class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong><strong>\u5f53\u4ece 1-plex \u6269\u5c55\u5230 6-plex \u65f6\uff0c\u5982\u4f55\u5c06 \u0394Ct \u964d\u81f3\u6700\u4f4e\uff1f<\/strong><\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/div><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p class=\"wp-block-paragraph\">\u8981\u5728\u5c06\u591a\u91cd qPCR \u4ece 1-plex \u6269\u5c55\u5230 6-plex \u65f6\u5c3d\u91cf\u51cf\u5c0f \u0394Ct\uff0c\u5e94\u4f7f\u7528\u4e13\u4e3a\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b\u8bbe\u8ba1\u7684\u63a2\u9488\u5316\u5b66\u4f53\u7cfb\u548c\u9884\u6df7\u6db2\u914d\u65b9\u3002\u9488\u5bf9\u591a\u91cd\u68c0\u6d4b\u4f18\u5316\u7684\u63a2\u9488\uff0c\u4f8b\u5982<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4482777\"><u>TaqMan QSY \u6216 QSY2 \u63a2\u9488<\/u><\/a>\u7ecf\u8fc7\u4e13\u95e8\u8bbe\u8ba1\uff0c\u53ef\u5728\u6269\u5c55\u8fc7\u7a0b\u4e2d\u5e2e\u52a9\u4fdd\u6301\u5206\u6790\u6027\u80fd\uff0c\u800c\u9884\u6df7\u6db2<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A58669\"><u>\u5982 TaqPath DuraPlex 1-Step RT-qPCR Master Mix<\/u><\/a>\u5219\u7ecf\u8fc7\u914d\u65b9\u4f18\u5316\uff0c\u4ee5\u652f\u6301\u591a\u4e2a\u9776\u6807\u7684\u540c\u6b65\u6269\u589e\u3002<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<style>.kt-accordion-id2217_9982a9-e8 .kt-accordion-inner-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:10px;}.kt-accordion-id2217_9982a9-e8 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;background:#ffffff;padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);}.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;background:#ffffff;color:#444444;padding-top:14px;padding-right:16px;padding-bottom:14px;padding-left:16px;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8 .kt-blocks-accordion-header:focus-visible{color:#444444;background:#ffffff;border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion--visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger, body:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id2217_9982a9-e8 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{color:#444444;background:#ffffff;border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id2217_9982a9-e8:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}@media all and (max-width: 1024px){.kt-accordion-id2217_9982a9-e8 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}}@media all and (max-width: 1024px){.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}}@media all and (max-width: 1024px){.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}}@media all and (max-width: 1024px){.kt-accordion-id2217_9982a9-e8 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}}@media all and (max-width: 767px){.kt-accordion-id2217_9982a9-e8 .kt-accordion-inner-wrap{display:block;}.kt-accordion-id2217_9982a9-e8 .kt-accordion-inner-wrap .kt-accordion-pane:not(:first-child){margin-top:10px;}.kt-accordion-id2217_9982a9-e8 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id2217_9982a9-e8 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-right-color:#878787;border-bottom-color:#878787;border-left-color:#878787;}.kt-accordion-id2217_9982a9-e8 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id2217_9982a9-e8 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-right-color:#eeeeee;border-bottom-color:#eeeeee;border-left-color:#0e9cd1;}}<\/style>\n<div class=\"wp-block-kadence-accordion alignnone\"><div class=\"kt-accordion-wrap kt-accordion-id2217_9982a9-e8 kt-accordion-has-2-panes kt-active-pane-0 kt-accordion-block kt-pane-header-alignment-left kt-accodion-icon-style-arrow kt-accodion-icon-side-right\" style=\"max-width:none\"><div class=\"kt-accordion-inner-wrap\" data-allow-multiple-open=\"false\" data-start-open=\"0\">\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-1 kt-pane2217_0d9182-69\"><div class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\">\u00a0<strong>\u5bf9\u4e8e 5-plex \u548c 6-plex qPCR\uff0c\u63a8\u8350\u4f7f\u7528\u54ea\u4e9b\u62a5\u544a\u67d3\u6599\uff1f<\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/div><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p class=\"wp-block-paragraph\">\u5bf9\u4e8e 5-plex \u548c 6-plex \u591a\u91cd qPCR\uff0c\u5e38\u7528 Cyanine 5\uff08Cy5\uff09\u548c Cyanine 5.5\uff08Cy5.5\uff09\u7b49\u62a5\u544a\u67d3\u6599\u6765\u6269\u5c55\u901a\u9053\u5bb9\u91cf\uff0c\u540c\u65f6\u6709\u52a9\u4e8e\u51cf\u5c11\u5149\u8c31\u91cd\u53e0\u3002\u8fd9\u4e9b\u67d3\u6599\u53ef\u88ab\u652f\u6301\u4e8e<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4482777\"><u>TaqMan QSY2 \u63a2\u9488\u5316\u5b66\u6280\u672f<\/u><\/a>\uff0c\u5176\u8bbe\u8ba1\u65e8\u5728\u5728\u66f4\u9ad8\u91cd\u6570\u6c34\u5e73\u4e0b\u4fdd\u6301\u4fe1\u53f7\u6e05\u6670\u5ea6\u548c\u6027\u80fd\u3002<\/p>\n\n\n\n<p class=\"wp-block-paragraph\">\u5e94\u59cb\u7ec8\u6839\u636e\u60a8\u7684\u4eea\u5668\u5149\u5b66\u914d\u7f6e\u4ee5\u53ca\u5404\u9776\u6807\u7684\u9884\u671f\u4e30\u5ea6\u6765\u786e\u8ba4\u67d3\u6599\u9009\u62e9\uff0c\u4ee5\u5b9e\u73b0\u6700\u4f73\u4fe1\u53f7\u5206\u79bb\u548c\u5b9a\u91cf\u51c6\u786e\u6027\u3002<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<p 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