{"id":564,"date":"2026-02-27T02:47:58","date_gmt":"2026-02-27T02:47:58","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=564"},"modified":"2026-03-09T05:55:03","modified_gmt":"2026-03-09T05:55:03","slug":"multiplex-vs-singleplex-qpcr-what-do-i-need-to-know","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/multiplex-vs-singleplex-qpcr-what-do-i-need-to-know\/","title":{"rendered":"\u591a\u91cd\u4e0e\u5355\u91cd qPCR"},"content":{"rendered":"\n<h2 class=\"wp-block-heading\" id=\"h-\u591a\u91cd\u6269\u589e\u7684\u4f18\u7f3a\u70b9\">\u591a\u91cd\u6269\u589e\u7684\u4f18\u7f3a\u70b9<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u5982\u4f55-\u4f55\u65f6\u4ee5\u53ca\u662f\u5426\u8981\u5bf9\u60a8\u7684qpcr\u5b9e\u9a8c\u8fdb\u884c\u591a\u91cd\u6269\u589e\">\u5982\u4f55\u3001\u4f55\u65f6\u4ee5\u53ca\u662f\u5426\u8981\u5bf9\u60a8\u7684qPCR\u5b9e\u9a8c\u8fdb\u884c\u591a\u91cd\u6269\u589e<\/h3>\n\n\n\n<p>\u5728\u8bbe\u8ba1\u5b9a\u91cfPCR\uff08qPCR\uff09\u5b9e\u9a8c\u65f6\uff0c\u6700\u5e38\u89c1\u7684\u95ee\u9898\u4e4b\u4e00\u5c31\u662f\u662f\u5426\u53ef\u4ee5\u5728\u5355\u6b21\u53cd\u5e94\u4e2d\u6269\u589e\u591a\u4e2a\u76ee\u6807\u5e8f\u5217\u3002\u8fd9\u88ab\u79f0\u4e3a\u591a\u91cd\u6269\u589e\uff0c\u597d\u6d88\u606f\u662f\u2014\u2014\u786e\u5b9e\u53ef\u4ee5\u5b9e\u73b0\u3002\u4e0d\u8fc7\uff0c\u8fd9\u4e5f\u5e26\u6765\u4e86\u4e00\u4e9b\u6311\u6218\uff0c\u9700\u8981\u89e3\u51b3\u8fd9\u4e9b\u95ee\u9898\u624d\u80fd\u786e\u4fdd\u5b9e\u9a8c\u6210\u529f\u3002\u5728Taq Talk\u89c6\u9891\u7cfb\u5217\u7684\u7b2c22\u548c23\u96c6\uff0c\u6211\u4eec\u56de\u987e\u4e86\u5173\u4e8e\u5982\u4f55\u3001\u4f55\u65f6\u4ee5\u53ca\u662f\u5426\u8981\u5bf9\u60a8\u7684qPCR\u5b9e\u9a8c\u8fdb\u884c\u591a\u91cd\u6269\u589e\u7684\u4e00\u4e9b\u5fc5\u5907\u77e5\u8bc6\u3002<\/p>\n\n\n<div class=\"wp-block-image\">\n<figure class=\"alignright\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/05\/Multiplex-vs-singleplex-blog-image-003-300x151.png\" alt=\"word cloud of multiplex and singleplex related terms\" class=\"wp-image-16694\" title=\"Multiplex vs Singleplex qPCR - What You Need to Know\" \/><\/figure>\n<\/div>\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u6bd4\u8f83\u4e0e\u5bf9\u6bd4\">\u6bd4\u8f83\u4e0e\u5bf9\u6bd4<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u5355\u91cd\u6269\u589e\">\u5355\u91cd\u6269\u589e<\/h3>\n\n\n\n<p>\u9996\u5148\uff0c\u5355\u91cd\u6269\u589e\uff08\u6bcf\u4e2a\u53cd\u5e94\u53ea\u6269\u589e\u4e00\u4e2a\u76ee\u6807\uff09\u8981\u6bd4<strong>\u7b80\u5355\u5f97\u591a<\/strong>\u591a\u91cd\u6269\u589e\u3002\u5b9e\u65f6PCR\u534f\u8bae\u901a\u5e38\u90fd\u662f\u56f4\u7ed5\u5355\u91cd\u5b9e\u9a8c\u8bbe\u8ba1\u7684\uff0c\u56e0\u6b64\u5207\u6362\u5230\u591a\u91cd\u6269\u589e\u9700\u8981\u5bf9\u534f\u8bae\u8fdb\u884c\u8c03\u6574\u3002\u5355\u91cd\u6269\u589e\u8fd8\u5177\u6709\u660e\u786e\u6027\uff0c\u5373\u6bcf\u4e2a\u53cd\u5e94\u53ea\u6269\u589e\u4e00\u4e2a\u76ee\u6807\u5e8f\u5217\uff0c\u4e0d\u4f1a\u51fa\u73b0\u4e0d\u540c\u7684\u6269\u589e\u4e8b\u4ef6\u76f8\u4e92\u5e72\u6270\u6216\u7ed3\u5408\uff0c\u4ece\u800c\u5bfc\u81f4\u7ed3\u679c\u590d\u6742\u5316\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u591a\u91cd\u6269\u589e\">\u591a\u91cd\u6269\u589e<\/h3>\n\n\n\n<p>\u591a\u91cd\u6269\u589e\u63d0\u4f9b\u4e86\u4e00\u4e9b\u91cd\u8981\u7684<strong>\u4f18\u52bf<\/strong>\uff0c\u8fd9\u4e9b\u4f18\u52bf\u7edd\u5bf9\u503c\u5f97\u8003\u8651\u3002\u5f53\u591a\u4e2a\u76ee\u6807\u5e8f\u5217\u5728\u540c\u4e00\u4e2a\u53cd\u5e94\u4e2d\u88ab\u6269\u589e\u65f6\uff0c\u53ef\u4ee5\u663e\u8457\u8282\u7701\u4e3b\u6df7\u5408\u8bd5\u5242\u3002\u5b54\u6570\u51cf\u5c11\u4e00\u534a\u6216\u66f4\u5c11\uff0c\u5c31\u610f\u5473\u7740\u603b\u67d3\u6599\u3001dNTP\u7b49\u7684\u7528\u91cf\u4e5f\u51cf\u5c11\u4e00\u534a\u2014\u2014\u8fd9\u79cd\u6210\u672c\u8282\u7ea6\u5bf9\u8bb8\u591a\u5b9e\u9a8c\u5ba4\u6765\u8bf4\u975e\u5e38\u6709\u5438\u5f15\u529b\u3002<\/p>\n\n\n\n<p>\u6b64\u5916\uff0c\u79fb\u6db2\u64cd\u4f5c\u6709\u65f6\u4f1a\u5bfc\u81f4\u6240\u6709qPCR\u53cd\u5e94\u4e4b\u95f4\u7684\u603b\u4f53\u79ef\u4e0d\u5b8c\u5168\u4e00\u81f4\u3002\u4f8b\u5982\uff0c\u5728\u5c06\u76ee\u6807\u57fa\u56e0\u4e0e\u53c2\u8003\u57fa\u56e0\u5206\u522b\u8fdb\u884c\u5355\u91cd\u6269\u589e\u5206\u6790\u65f6\uff0c\u8fd9\u53ef\u80fd\u6210\u4e3a\u4e00\u4e2a\u95ee\u9898\u3002<\/p>\n\n\n\n<p>\u5728\u4e00\u6b21<strong>\u591a\u91cd\u6269\u589e<\/strong>\u5b9e\u9a8c\u4e2d\uff0c\u591a\u4e2a\u57fa\u56e0\u5728\u540c\u4e00\u4e2a\u53cd\u5e94\u4e2d\u88ab\u5206\u6790\uff0c\u56e0\u6b64\u5404\u76ee\u6807\u4e4b\u95f4\u7684\u4f53\u79ef\u5fc5\u987b\u76f8\u540c\u3002\u9700\u8981\u586b\u5145\u7684\u5b54\u6570\u66f4\u5c11\uff0c\u4f7f\u5f97\u591a\u91cd\u6269\u589e\u5b9e\u9a8c\u7684\u8bbe\u7f6e\u901f\u5ea6\u8fdc\u5feb\u4e8e\u7b49\u91cf\u5355\u91cd\u6269\u589e\u53cd\u5e94\u7684\u8bbe\u7f6e\u3002<\/p>\n\n\n\n<p>\u7136\u800c\uff0c\u7531\u4e8eqPCR\u901a\u5e38\u5e94\u8be5\u91c7\u7528\u76f8\u540c\u6761\u4ef6\u4e0b\u7684\u4e09\u91cd\u590d\u53cd\u5e94\uff0c\u5373\u4f7f\u662f\u5355\u91cd\u6269\u589e\u5b9e\u9a8c\u4e5f\u5185\u7f6e\u4e86\u76d1\u63a7\u79fb\u6db2\u7cbe\u5ea6\u7684\u65b9\u6cd5\u3002\u56e0\u6b64\uff0c\u591a\u91cd\u6269\u589e\u5f88\u5c11\uff08\u751a\u81f3\u51e0\u4e4e\u4e0d\u4f1a\uff09\u6210\u4e3a\u5f3a\u5236\u8981\u6c42\uff0c\u4f46\u5982\u679c\u80fd\u591f\u59a5\u5584\u5904\u7406\u5176\u590d\u6742\u6027\uff0c\u5b83\u4ecd\u7136\u53ef\u4ee5\u5e26\u6765\u5927\u91cf\u7684<strong>\u8bd5\u5242\u548c\u65f6\u95f4\u4e0a\u7684\u8282\u7701\u3002<\/strong><br><br><strong>\u76f8\u5173\u89c6\u9891: <\/strong><a title=\"Considerations for Singleplex vs. Multiplex qPCR\" href=\"https:\/\/players.brightcove.net\/3663210762001\/default_default\/index.html?videoId=6298814638001\" target=\"_blank\" rel=\"noopener\">\u5355\u91cd\u4e0e\u591a\u91cdqPCR\u7684\u6ce8\u610f\u4e8b\u9879\u2014\u2014Taq Talk\u7b2c23\u96c6<\/a><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4f18\u5316\u591a\u91cdqpcr\u5b9e\u9a8c\">\u4f18\u5316\u591a\u91cdqPCR\u5b9e\u9a8c<\/h2>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u76ee\u6807\u5e8f\u5217\u8981\u6c42\">\u76ee\u6807\u5e8f\u5217\u8981\u6c42<\/h3>\n\n\n\n<p>\u8981\u4ece\u591a\u91cd\u5b9e\u9a8c\u4e2d\u83b7\u5f97\u4f18\u8d28\u6570\u636e\uff0c\u9700\u8981\u7279\u522b\u5c0f\u5fc3\u3002\u6bcf\u4e2a\u76ee\u6807\u5e8f\u5217\u90fd\u9700\u8981\u4e13\u5c5e\u7684\u67d3\u6599\u548c\u76f8\u5e94\u7684\u63a2\u9488\uff0c\u5e76\u4e14\u8be5\u67d3\u6599\u5fc5\u987b\u5177\u6709\u72ec\u7279\u7684\u53d1\u5c04\u6ce2\u957f\uff0c\u4ee5\u4fbf\u60a8\u7684qPCR\u4eea\u5668\u80fd\u591f\u68c0\u6d4b\u5e76\u533a\u5206\u3002\u67d3\u6599\u9009\u62e9\u4e5f\u975e\u5e38\u91cd\u8981\uff1aApplied Biosystems\u2122 SYBR\u2122 Green\u8bd5\u5242\u76d2\u4ec5\u9002\u7528\u4e8e\u5355\u91cd\u53cd\u5e94\uff0c\u56e0\u4e3aSYBR Green\u67d3\u6599\u4f1a\u7ed3\u5408\u6240\u6709PCR\u4ea7\u7269\u3002\u800c\u4f7f\u7528Applied Biosystems\u2122 TaqMan\u2122 Assays\u65f6\uff0c\u53ef\u4ee5\u901a\u8fc7\u6807\u8bb0\u4e0d\u540c\u8367\u5149\u57fa\u56e2\uff08\u5982FAM\u2122\u548cVIC\u2122\u67d3\u6599\uff09\u7684\u63a2\u9488\u8f7b\u677e\u533a\u5206\u5404\u4e2a\u76ee\u6807\u3002\u4efb\u4f55Applied Biosystems\u2122\u5b9e\u65f6PCR\u4eea\u5668\u90fd\u80fd\u533a\u5206\u8fd9\u4e9b\u67d3\u6599\u3002<\/p>\n\n\n\n<p>\u4f46\u8bf7\u6ce8\u610f\u2014\u2014\u4ec5\u4ec5\u5c06\u6240\u6709\u8fd9\u4e9b\u7ec4\u5206\u653e\u5728\u4e00\u4e2a\u53cd\u5e94\u4f53\u7cfb\u4e2d\uff0c\u53ef\u80fd\u8fd8\u4e0d\u8db3\u4ee5\u83b7\u5f97\u4f18\u8d28\u6570\u636e\u3002\u5f53\u4e24\u4e2a\u53cd\u5e94\u5171\u4eab\u4e00\u4e2a\u5b54\u65f6\uff0c\u5373\u4f7f\u5b83\u4eec\u62e5\u6709\u4e0d\u540c\u7684\u63a2\u9488\uff0c\u4e5f\u4f1a\u4e89\u593adNTP\u3001\u9176\u4ee5\u53ca\u5176\u4ed6\u53cd\u5e94\u7ec4\u5206\uff08\u89c1\u56fe1\uff09\u3002<\/p>\n\n\n\n<p>\u5982\u679c\u67d0\u4e2a\u57fa\u56e0\uff08\u901a\u5e38\u662f\u5185\u6e90\u6027\u5bf9\u7167\uff09\u6bd4\u53e6\u4e00\u4e2a\u5148\u5f00\u59cb\u6269\u589e\uff0c\u5b83\u53ef\u80fd\u5728\u53e6\u4e00\u4e2a\u57fa\u56e0\u51fa\u73b0\u5728\u6269\u589e\u66f2\u7ebf\u4e4b\u524d\u5c31\u8fdb\u5165\u7ebf\u6027\u548c\u5e73\u53f0\u671f\u3002\u6b64\u65f6\uff0c\u76ee\u6807\u57fa\u56e0\u4f1a\u56e0\u8bd5\u5242\u4f9b\u5e94\u4e0d\u8db3\u800c\u6269\u589e\u6548\u679c\u8f83\u5dee\uff0c\u5bfc\u81f4\u7ed3\u679c\u65e0\u6cd5\u89e3\u91ca\uff08\u89c1\u56fe2\uff09\u3002<\/p>\n\n\n<div class=\"wp-block-image wp-image-16695 size-full\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/05\/Fig-1.-Taq-Talk-Ep-23-01.12.png\" alt=\"figure 1 competition for reaction components in real-time PCR assay with reaction wells\" class=\"wp-image-16695\" \/><figcaption class=\"wp-element-caption\">\u56fe1. \u5b9e\u65f6PCR\u5206\u6790\u4e2d\u5bf9\u53cd\u5e94\u7ec4\u5206\u7684\u7ade\u4e89\u3002\u4e2d\u5fc3\u663e\u793a\u7684\u662f\u4e09\u91cd\u590d\u53cd\u5e94\u5b54\u3002<\/figcaption><\/figure>\n<\/div>\n\n<div class=\"wp-block-image wp-image-16696 size-full\">\n<figure class=\"aligncenter\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/05\/Fig-2.-Taq-Talk-Ep-22-01.58.png\" alt=\"figure 2 early amplification of endogenous control resulting in poor amplification of target gene\" class=\"wp-image-16696\" \/><figcaption class=\"wp-element-caption\">\u56fe2. \u5185\u6e90\u6027\u5bf9\u7167\u63d0\u524d\u6269\u589e\u5bfc\u81f4\u76ee\u6807\u57fa\u56e0\u6269\u589e\u6548\u679c\u4e0d\u4f73\u3002<\/figcaption><\/figure>\n<\/div>\n\n\n<p><strong>\u76f8\u5173\u89c6\u9891:<\/strong><a title=\"How to Optimize Multiplex qPCR Experiments\" href=\"https:\/\/players.brightcove.net\/3663210762001\/default_default\/index.html?videoId=6298812105001\" target=\"_blank\" rel=\"noopener\">\u5982\u4f55\u4f18\u5316\u591a\u91cdqPCR\u5b9e\u9a8c\u2014\u2014Taq Talk\u7b2c22\u96c6<\/a><br><br>\n\n\u4f60\u5f88\u5e78\u8fd0\u2014\u2014\u907f\u514d\u8fd9\u4e2a\u95ee\u9898\u5176\u5b9e\u975e\u5e38\u7b80\u5355\u3002\u5982\u679c\u67d0\u4e2a\u76ee\u6807\u53ef\u80fd\u4f1a\u5728\u8bd5\u5242\u7ade\u4e89\u4e2d\u80dc\u8fc7\u53e6\u4e00\u4e2a\u76ee\u6807\uff0c\u53ef\u4ee5\u5bf9\u8be5\u76ee\u6807\u8fdb\u884c\u5f15\u7269\u9650\u5236\u3002\u663e\u8457\u964d\u4f4e\u5bf9\u7167\u57fa\u56e0\u7684\u5f15\u7269\u6d53\u5ea6\uff0c\u4f1a\u8ba9\u5176\u4fe1\u53f7\u66f4\u65e9\u8fbe\u5230\u5e73\u53f0\u671f\uff0c\u4ece\u800c\u66f4\u5bb9\u6613\u533a\u5206\u4e24\u4e2a\u4fe1\u53f7\uff0c\u5e76\u4e3a\u611f\u5174\u8da3\u7684\u5b9e\u9a8c\u57fa\u56e0\u4fdd\u7559\u66f4\u591a\u5176\u4ed6\u8bd5\u5242\u3002\n<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u53cc\u57fa\u56e0\u591a\u91cd\u68c0\u6d4b\">\u53cc\u57fa\u56e0\u591a\u91cd\u68c0\u6d4b<\/h3>\n\n\n\n<p>\u8981\u5224\u65ad\u4e24\u79cd\u57fa\u56e0\u591a\u91cd\u68c0\u6d4b\u7684\u96be\u6613\u7a0b\u5ea6\u3001\u9009\u62e9\u54ea\u4e2a\u57fa\u56e0\u8fdb\u884c\u5f15\u7269\u9650\u5236\uff0c\u4ee5\u53ca\u4e24\u79cd\u67d3\u6599\u662f\u5426\u771f\u6b63\u517c\u5bb9\u591a\u91cd\u68c0\u6d4b\uff0c\u9700\u8981\u4e00\u4e9b\u6d4b\u8bd5\u6837\u672c\u3002\u9996\u5148\u4ece\u4f60\u7684\u5b9e\u9a8c\u7ec4\u548c\u5bf9\u7167\u7ec4\u5404\u90095-6\u4e2a\u6837\u672c\uff0c\u5728\u53cc\u91cd\u548c\u5355\u91cd\u914d\u7f6e\u4e0b\u5206\u522b\u8fd0\u884c\u3002\u5982\u679c\u5927\u591a\u6570\u6837\u672c\u5728\u4e24\u79cd\u914d\u7f6e\u4e0b\u7684\u8bfb\u6570\u5dee\u5f02\u5f88\u5c0f\uff0c\u5219\u53ef\u4ee5\u5b89\u5168\u5730\u5c06\u5269\u4f59\u5b9e\u9a8c\u4ee5\u591a\u91cd\u65b9\u5f0f\u8fdb\u884c\u3002\u5982\u679c\u4e24\u79cd\u8bfb\u6570\u4e0d\u4e00\u81f4\uff0c\u8bf4\u660e\u4ee5\u591a\u91cd\u65b9\u5f0f\u8fd0\u884c\u5b9e\u9a8c\u53ef\u80fd\u5bfc\u81f4\u6570\u636e\u4e0d\u7a33\u5b9a\u751a\u81f3\u65e0\u6cd5\u4f7f\u7528\u3002\u5982\u679c\u6837\u672c\u6570\u91cf\u6709\u9650\u6216\u5236\u5907\u8017\u65f6\u8f83\u957f\uff0c\u907f\u514d\u98ce\u9669\u53ef\u80fd\u66f4\u4e3a\u53ef\u53d6\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b\">\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b<\/h3>\n\n\n\n<p>\n\u5728\u9ad8\u9636\u591a\u91cd\u68c0\u6d4b\u4e2d\u4e5f\u5e94\u4fdd\u6301\u540c\u6837\u7684\u8c28\u614e\u6001\u5ea6\u3002<a title=\"Highly efficient Applied Biosystems real-time PCR solutions\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr.html\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122\u5b9e\u65f6PCR\u4eea\u5668<\/a>\u914d\u5907\u4e86\u591a\u4e2a\u6ee4\u5149\u7247\u7ec4\uff0c\u53ef\u8ba9\u4f60\u5728\u5355\u6b21\u53cd\u5e94\u4e2d\u5b9e\u73b0\u591a\u4e2a\u5f15\u7269\u548c\u63a2\u9488\u7684\u591a\u91cd\u68c0\u6d4b\u3002\u7136\u800c\uff0c\u5355\u6b21\u53cd\u5e94\u4e2d\u7684\u76ee\u6807\u8d8a\u591a\uff0c\u5b83\u4eec\u4e4b\u95f4\u7684\u76f8\u4e92\u4f5c\u7528\u5c31\u8d8a\u590d\u6742\uff0c\u7ed3\u679c\u88ab\u67d0\u79cd\u65b9\u5f0f\u504f\u79bb\u7684\u98ce\u9669\u4e5f\u8d8a\u5927\u3002<br><br>\n\n<a title=\"Applied Biosystems TaqMan Assays are the industry-leading choice for 5' nuclease qPCR assays\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays.html\" target=\"_blank\" rel=\"noopener\">Applied Biosystems\u2122 TaqMan\u2122 \u68c0\u6d4b\u7cfb\u7edf<\/a>\u91c7\u7528\u5f3a\u5927\u3001\u9ad8\u5ea6\u7279\u5f02\u4e14\u6781\u5176\u7075\u6d3b\u7684\u5b9e\u65f6PCR\u6280\u672f\u3002\u7528\u6237\u53ef\u4ee5\u9009\u62e9\u4e0d\u540c\u67d3\u6599\u7684TaqMan\u63a2\u9488\uff0c\u8fd9\u4f7f\u4ed6\u4eec\u80fd\u591f\u81ea\u4fe1\u4e14\u6210\u529f\u5730\u5728\u5355\u5b54\u5185\u5b9e\u73b0\u591a\u4e2a\u76ee\u6807\u7684\u591a\u91cd\u68c0\u6d4b\u3002\n<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4e86\u89e3\u66f4\u591a\">\u4e86\u89e3\u66f4\u591a<\/h2>\n\n\n\n<p><a title=\"Discover the Real-Time PCR Handbook 2.0 Digital Experience\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/global\/forms\/life-science\/digital-experience.html\" target=\"_blank\" rel=\"noopener\">\u00bb \u5b9e\u65f6PCR\u6570\u5b57\u624b\u518c2.0<\/a> \u2013 \u63a2\u7d22\u5b9e\u65f6PCR\u7684\u6700\u4f73\u5b9e\u8df5\u3001\u57fa\u672c\u539f\u7406\u548c\u6545\u969c\u6392\u9664\u6280\u5de7\u3002\n<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-applied-biosystems-taq-talk\u89c6\u9891\u7cfb\u5217\">Applied Biosystems\u2122 Taq Talk\u89c6\u9891\u7cfb\u5217<\/h3>\n\n\n\n<p><a title=\"Taq Talk Video Series - Whether you're a beginner or a seasoned veteran in the lab, you probably have questions about real-time PCR\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-learning-center\/real-time-pcr-basics\/taq-talk-video-series.html\" target=\"_blank\" rel=\"noopener\">\u00bb \u67e5\u770b\u5b8c\u6574\u7684Applied Biosystems Taq Talk\u89c6\u9891\u7cfb\u5217<\/a> \u2013 \u4f5c\u4e3a\u5b9e\u65f6PCR\u9886\u57df\u7684\u8d44\u6df1\u4e13\u4e1a\u4eba\u58eb\uff0c\u6211\u4eec\u5c06\u5728\u8fd9\u4e2a\u5168\u65b0\u7684\u89c6\u9891\u7cfb\u5217\u4e2d\u89e3\u7b54\u60a8\u6700\u5e38\u89c1\u7684\u5b9e\u65f6PCR\u95ee\u9898\u548c\u6311\u6218\u3002\u6d4f\u89c8\u8282\u76ee\u5355\u5e76\u5173\u6ce8\u66f4\u591aTaq Talk\u89c6\u9891\u66f4\u65b0.\n<\/p>\n\n\n\n<h4 class=\"wp-block-heading\" id=\"h-\u89c2\u770b\u672c\u6587\u5f15\u7528\u7684\u89c6\u9891\">\u89c2\u770b\u672c\u6587\u5f15\u7528\u7684\u89c6\u9891<\/h4>\n\n\n\n<p><a title=\"How to Optimize Multiplex qPCR Experiments\" href=\"https:\/\/players.brightcove.net\/3663210762001\/default_default\/index.html?videoId=6298812105001\" target=\"_blank\" rel=\"noopener\">\u00bb \u5982\u4f55\u4f18\u5316\u591a\u91cdqPCR\u5b9e\u9a8c \u2013 Taq Talk\u7b2c22\u96c6<\/a><br><br>\n<a title=\" Considerations for Singleplex vs. Multiplex qPCR \" href=\"https:\/\/players.brightcove.net\/3663210762001\/default_default\/index.html?videoId=6298814638001\" target=\"_blank\" rel=\"noopener\">\u00bb \u5355\u91cd\u4e0e\u591a\u91cdqPCR\u5b9e\u9a8c\u6ce8\u610f\u4e8b\u9879 \u2013 Taq Talk\u7b2c23\u96c6<\/a><br><br>\n<strong>&#8212;<\/strong><br><br>\n<strong>\u4ec5\u4f9b\u79d1\u7814\u4f7f\u7528\u3002\u4e0d\u5f97\u7528\u4e8e\u8bca\u65ad\u7a0b\u5e8f\u3002<\/strong><br><br>\n\n\u00a9 2022 Thermo Fisher Scientific Inc. \u4fdd\u7559\u6240\u6709\u6743\u5229\u3002\u9664\u975e\u53e6\u6709\u8bf4\u660e\uff0c\u6240\u6709\u5546\u6807\u5747\u4e3aThermo Fisher Scientific\u53ca\u5176\u5b50\u516c\u53f8\u7684\u8d22\u4ea7\u3002TaqMan\u662fRoche Molecular Systems, Inc.\u7684\u5546\u6807\uff0c\u7ecf\u8bb8\u53ef\u4f7f\u7528\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/support\/contact-us-cn.html?icid=blog_banner#form\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg\" alt=\"contact and techincal support\" class=\"wp-image-387\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/02\/779-\u00d7-134-x3-1-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n","protected":false},"excerpt":{"rendered":"<p>\u591a\u91cd\u6269\u589e\u7684\u4f18\u7f3a\u70b9 \u5982\u4f55\u3001\u4f55\u65f6\u4ee5\u53ca\u662f\u5426\u8981\u5bf9\u60a8\u7684qPCR\u5b9e\u9a8c\u8fdb\u884c\u591a\u91cd\u6269\u589e \u5728\u8bbe\u8ba1\u5b9a\u91cfPCR\uff08qPCR\uff09\u5b9e\u9a8c\u65f6\uff0c\u6700\u5e38<\/p>\n","protected":false},"author":1852,"featured_media":565,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","footnotes":""},"categories":[8],"tags":[16,17],"division":[10],"class_list":{"0":"post-564","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-8","8":"tag-qpcr","9":"tag-pcr","10":"division-gsd","11":"entry"},"_selected_authors":[744],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- 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