{"id":570,"date":"2026-03-03T03:08:24","date_gmt":"2026-03-03T03:08:24","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=570"},"modified":"2026-03-09T05:48:14","modified_gmt":"2026-03-09T05:48:14","slug":"deconvolution-101","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/deconvolution-101\/","title":{"rendered":"\u663e\u5fae\u56fe\u50cf\u53bb\u5377\u79ef\uff1a\u6e05\u6670\u663e\u5fae\u955c\u6307\u5357"},"content":{"rendered":"\n<figure class=\"wp-block-image size-full\"><img decoding=\"async\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/Title-Card-Deconvo-1-1.png\" alt=\"Deconvolution 101: Guide to Crisp Microscopy\" class=\"wp-image-4843\" \/><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>\u663e\u5fae\u955c\u6781\u5927\u5730\u63d0\u5347\u4e86\u6211\u4eec\u89c2\u5bdf\u4e0d\u53ef\u89c1\u4e16\u754c\u7684\u80fd\u529b\uff0c\u4f46\u5373\u4f7f\u662f\u9ad8\u8d28\u91cf\u7684\u56fe\u50cf\u4e5f\u53ef\u80fd\u56e0\u5149\u5b66\u7cfb\u7edf\u548c\u5149\u7684\u7269\u7406\u7279\u6027\u800c\u51fa\u73b0\u6a21\u7cca\u3002\u8fd9\u79cd\u6a21\u7cca\u6216\u80cc\u666f\u4fe1\u53f7\u4f1a\u8ba9\u89c2\u5bdf\u7ec6\u5fae\u7ed3\u6784\u53d8\u5f97\u56f0\u96be\u3002<\/p>\n\n\n\n<p>\u56fe\u50cf\u53cd\u5377\u79ef\u662f\u4e00\u79cd\u8ba1\u7b97\u6280\u672f\uff0c\u53ef\u4ee5\u5e2e\u52a9\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\uff0c\u901a\u8fc7\u51cf\u5c11\u80cc\u666f\u5e76\u589e\u5f3a\u56fe\u50cf\u6e05\u6670\u5ea6\u3002<\/p>\n\n\n\n<p>\u5728\u8fd9\u7bc7\u535a\u5ba2\u6587\u7ae0\u4e2d\uff0c\u6211\u4eec\u5c06\u63a2\u8ba8\u56fe\u50cf\u53cd\u5377\u79ef\u7684\u57fa\u7840\u77e5\u8bc6\u3001\u5b83\u5982\u4f55\u6539\u5584\u663e\u5fae\u955c\u56fe\u50cf\uff0c\u4ee5\u53ca\u4e00\u4e9b\u4f60\u53ef\u4ee5\u7528\u6765\u5904\u7406\u81ea\u5df1\u6570\u636e\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/evos-cell-imaging-systems\/models\/evos-m7000.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>\u4f7f\u7528EVOS M7000\u6210\u50cf\u7cfb\u7edf\u63a2\u7d22\u53cd\u5377\u79ef\u6280\u672f<\/strong><\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-yoast-seo-table-of-contents yoast-table-of-contents\"><h2>\u76ee\u5f55<\/h2><ul><li><a href=\"#h-\u4ec0\u4e48\u662f\u53cd\u5377\u79ef\" data-level=\"2\">\u4ec0\u4e48\u662f\u53cd\u5377\u79ef\uff1f<\/a><\/li><li><a href=\"#h-\u4e3a\u4ec0\u4e48\u56fe\u50cf\u53cd\u5377\u79ef\u6709\u7528-\u5b83\u80fd\u4e3a\u4f60\u5e26\u6765\u4ec0\u4e48\" data-level=\"2\">\u4e3a\u4ec0\u4e48\u56fe\u50cf\u53cd\u5377\u79ef\u6709\u7528\uff0c\u5b83\u80fd\u4e3a\u4f60\u5e26\u6765\u4ec0\u4e48\uff1f<\/a><ul><li><a href=\"#h-\u53cd\u5377\u79ef\u7684\u4f18\u52bf\" data-level=\"3\">\u53cd\u5377\u79ef\u7684\u4f18\u52bf<\/a><\/li><li><a href=\"#h-\u53cd\u5377\u79ef\u663e\u5fae\u955c\u7684\u5e94\u7528\" data-level=\"3\">\u53cd\u5377\u79ef\u663e\u5fae\u955c\u7684\u5e94\u7528<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u53bb\u5377\u79ef\u662f\u5982\u4f55\u5de5\u4f5c\u7684\" data-level=\"2\">\u53bb\u5377\u79ef\u662f\u5982\u4f55\u5de5\u4f5c\u7684\uff1f<\/a><ul><li><a href=\"#h-\u70b9\u6269\u6563\u51fd\u6570\" data-level=\"3\">\u70b9\u6269\u6563\u51fd\u6570<\/a><\/li><li><a href=\"#h-\u53bb\u5377\u79ef\u7b97\u6cd5\" data-level=\"3\">\u53bb\u5377\u79ef\u7b97\u6cd5<\/a><\/li><\/ul><\/li><li><a href=\"#h-2d\u56fe\u50cf\u53bb\u5377\u79ef\u76845\u4e2a\u793a\u4f8b-\u5904\u7406\u524d\u4e0e\u5904\u7406\u540e\u5bf9\u6bd4\" data-level=\"2\">2D\u56fe\u50cf\u53bb\u5377\u79ef\u76845\u4e2a\u793a\u4f8b\uff0c\u5904\u7406\u524d\u4e0e\u5904\u7406\u540e\u5bf9\u6bd4<\/a><ul><li><a href=\"#h-1-\u5c0f\u9f20\u80be\u810f\" data-level=\"3\">1. \u5c0f\u9f20\u80be\u810f<\/a><\/li><li><a href=\"#h-2-\u5c0f\u9f20\u7ed3\u80a0\" data-level=\"3\">2. \u5c0f\u9f20\u7ed3\u80a0 \ud83d\udd17<\/a><\/li><li><a href=\"#h-3-hela\u7ec6\u80de\" data-level=\"3\">3. HeLa\u7ec6\u80de<\/a><\/li><li><a href=\"#h-4-bpae\u7ec6\u80de\" data-level=\"3\">4. BPAE\u7ec6\u80de<\/a><\/li><li><a href=\"#h-5-u2os\u7ec6\u80de\" data-level=\"3\">5. U2OS\u7ec6\u80de<\/a><\/li><\/ul><\/li><li><a href=\"#h-\u66f4\u591a\u53bb\u5377\u79ef\u5b66\u4e60\u8d44\u6e90\" data-level=\"2\">\u66f4\u591a\u53bb\u5377\u79ef\u5b66\u4e60\u8d44\u6e90<\/a><\/li><\/ul><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u4ec0\u4e48\u662f\u53cd\u5377\u79ef\">\u4ec0\u4e48\u662f\u53cd\u5377\u79ef\uff1f<\/h2>\n\n\n\n<p>\u5f53\u4f60\u4f7f\u7528\u8367\u5149\u663e\u5fae\u955c\uff0c\u5c24\u5176\u662f\u5bbd\u573a\u663e\u5fae\u955c\u65f6\uff0c\u4f60\u53ef\u80fd\u4f1a\u6ce8\u610f\u5230\u4f60\u7684\u56fe\u50cf\u770b\u8d77\u6765\u6709\u4e9b\u6a21\u7cca\uff0c\u6216\u8005\u663e\u793a\u51fa\u80cc\u666f\u4fe1\u53f7\u3002\u8fd9\u79cd\u6a21\u7cca\u5e76\u4e0d\u662f\u4f60\u7684\u64cd\u4f5c\u5931\u8bef\uff0c\u800c\u662f\u7531\u4e8e\u6837\u54c1\u4e2d\u4e0d\u540c\u7126\u5e73\u9762\u4e0a\u7684\u79bb\u7126\u5149\u7ebf\u5171\u540c\u4f5c\u7528\u4e8e\u56fe\u50cf\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u6765\u81ea\u5f53\u524d\u7126\u5e73\u9762\u4e0a\u4e0b\u7ed3\u6784\u7684\u5149\u7ebf\u90fd\u4f1a\u88ab\u6355\u6349\uff0c\u4ece\u800c\u964d\u4f4e\u4e86\u4f60\u5173\u6ce8\u7ed3\u6784\u7684\u6e05\u6670\u5ea6\u3002<\/p>\n\n\n\n<p>\u53bb\u5377\u79ef\u662f\u4e00\u79cd\u8ba1\u7b97\u6280\u672f\uff0c\u53ef\u4ee5\u5e2e\u52a9\u89e3\u51b3\u8fd9\u4e2a\u95ee\u9898\u3002\u5b83\u5229\u7528\u663e\u5fae\u955c\u7684\u70b9\u6269\u6563\u51fd\u6570\uff08PSF\uff09\u2014\u2014\u5373\u5355\u4e2a\u5149\u70b9\u5728\u6210\u50cf\u7cfb\u7edf\u4e2d\u7684\u8868\u73b0\u6a21\u578b\u2014\u2014\u901a\u8fc7\u6570\u5b66\u65b9\u5f0f\u5c06\u79bb\u7126\u5149\u91cd\u65b0\u5206\u914d\u56de\u5176\u539f\u59cb\u6765\u6e90\u3002\u8fd9\u6837\uff0c\u53bb\u5377\u79ef\u80fd\u591f\u589e\u5f3a\u56fe\u50cf\u7684\u5bf9\u6bd4\u5ea6\u548c\u5206\u8fa8\u7387\uff0c\u4f7f\u4f60\u66f4\u5bb9\u6613\u533a\u5206\u6837\u54c1\u4e2d\u7684\u7ec6\u8282\u7ed3\u6784\u3002<\/p>\n\n\n\n<p>\u76ee\u524d\u6709\u591a\u79cd\u7528\u4e8e\u56fe\u50cf\u53bb\u5377\u79ef\u7684\u7b97\u6cd5\uff0c\u4ece\u7b80\u5355\u7684\u53bb\u6a21\u7cca\u65b9\u6cd5\u5230\u66f4\u590d\u6742\u7684\u8fed\u4ee3\u5904\u7406\u3002\u4e00\u4e9b\u5148\u8fdb\u6280\u672f\u751a\u81f3\u7ed3\u5408\u4e86\u6df1\u5ea6\u5b66\u4e60\uff0c\u4ee5\u63d0\u5347PSF\u4f30\u7b97\u548c\u53bb\u5377\u79ef\u8fc7\u7a0b\u672c\u8eab\u7684\u51c6\u786e\u6027\u3002<\/p>\n\n\n\n<p>\u5728\u5b9e\u9645\u64cd\u4f5c\u4e2d\uff0c\u5bf9\u663e\u5fae\u955c\u56fe\u50cf\u5e94\u7528\u53bb\u5377\u79ef\u53ef\u4ee5\u663e\u8457\u63d0\u5347\u56fe\u50cf\u8d28\u91cf\uff0c\u56e0\u6b64\u5bf9\u4e8e\u5e0c\u671b\u5145\u5206\u5229\u7528\u8367\u5149\u663e\u5fae\u6570\u636e\u7684\u4eba\u6765\u8bf4\uff0c\u8fd9\u662f\u4e00\u9879\u975e\u5e38\u6709\u4ef7\u503c\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n<div class=\"wp-block-image\">\n<figure data-wp-context=\"{&quot;imageId&quot;:&quot;6aa7e805794f5&quot;}\" data-wp-interactive=\"core\/image\" data-wp-key=\"6aa7e805794f5\" class=\"aligncenter size-full wp-lightbox-container\"><img decoding=\"async\" data-wp-class--hide=\"state.isContentHidden\" data-wp-class--show=\"state.isContentVisible\" data-wp-init=\"callbacks.setButtonStyles\" data-wp-on--click=\"actions.showLightbox\" data-wp-on--load=\"callbacks.setButtonStyles\" data-wp-on-window--resize=\"callbacks.setButtonStyles\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/D1-Image.png\" alt=\"Deconvolution of a widefield HeLa cell image with fluorescent staining shows a much clearer image of cellular structure\" class=\"wp-image-4813\" 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id=\"h-\u53cd\u5377\u79ef\u7684\u4f18\u52bf\">\u53cd\u5377\u79ef\u7684\u4f18\u52bf<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6539\u5584\u56fe\u50cf\u6e05\u6670\u5ea6<\/strong>: \u53bb\u5377\u79ef\u7b97\u6cd5\u5c06\u79bb\u7126\u5149\u91cd\u65b0\u5206\u914d\u5230\u5176\u6765\u6e90\uff0c\u4ece\u800c\u83b7\u5f97\u66f4\u6e05\u6670\u7684\u56fe\u50cf\uff0c\u5e76\u63d0\u5347\u5bf9\u6bd4\u5ea6\u548c\u5206\u8fa8\u7387\u3002<\/li>\n\n\n\n<li><strong>\u589e\u5f3a\u7684\u4fe1\u566a\u6bd4\uff08SNR\uff09<\/strong>: \u901a\u8fc7\u51cf\u5c11\u80cc\u666f\u566a\u58f0\uff0c\u53bb\u5377\u79ef\u63d0\u9ad8\u4e86\u4fe1\u566a\u6bd4\uff0c\u4f7f\u68c0\u6d4b\u548c\u5206\u6790\u5fae\u5f31\u4fe1\u53f7\u53d8\u5f97\u66f4\u52a0\u5bb9\u6613\u3002<\/li>\n\n\n\n<li><strong>\u66f4\u597d\u7684\u4e09\u7ef4\u53ef\u89c6\u5316<\/strong>: \u5e94\u7528\u4e8eZ\u8f74\u5806\u53e0\u65f6\uff0c\u53bb\u5377\u79ef\u4f18\u5316\u4e86\u8f74\u5411\u5206\u8fa8\u7387\uff0c\u4e3a\u6837\u54c1\u63d0\u4f9b\u66f4\u51c6\u786e\u7684\u4e09\u7ef4\u91cd\u5efa\u3002<\/li>\n\n\n\n<li><strong>\u5b9a\u91cf\u7cbe\u786e\u6027<\/strong>: \u66f4\u6e05\u6670\u7684\u56fe\u50cf\u53ef\u4ee5\u5e26\u6765\u5bf9\u7ed3\u6784\u3001\u5f3a\u5ea6\u548c\u7a7a\u95f4\u5173\u7cfb\u7684\u66f4\u7cbe\u786e\u6d4b\u91cf\u3002<\/li>\n<\/ul>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u53cd\u5377\u79ef\u663e\u5fae\u955c\u7684\u5e94\u7528\">\u53cd\u5377\u79ef\u663e\u5fae\u955c\u7684\u5e94\u7528<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6d3b\u7ec6\u80de\u6210\u50cf<\/strong>: \u589e\u5f3a<a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/evos-cell-imaging-systems\/models\/evos-m7000.html?ef_id=Cj0KCQjwlYHBBhD9ARIsALRu09rF31bmEgaGzZDvzK0OQTOkySXPh-CMw9FC-FNh6tkZ-hkJWZ80jbMaAmtlEALw_wcB:G:s&amp;s_kwcid=AL!3652!3!586406504876!e!!g!!thermo%20fisher%20evos%20m7000!9768035657!98974177639&amp;cid=bid_pca_iie_r01_co_cp1359_pjt0000_bid00000_0se_gaw_bt_lgn_ins&amp;gad_source=1&amp;gad_campaignid=9768035657&amp;gbraid=0AAAAADxi_GSw2-gt0I0edtgM0eeBwqgoz&amp;gclid=Cj0KCQjwlYHBBhD9ARIsALRu09rF31bmEgaGzZDvzK0OQTOkySXPh-CMw9FC-FNh6tkZ-hkJWZ80jbMaAmtlEALw_wcB#media\">\u5ef6\u65f6\u5e8f\u5217<\/a>\u901a\u8fc7\u51cf\u5c11\u6a21\u7cca\uff0c\u4f7f\u52a8\u6001\u8fc7\u7a0b\u7684\u89c2\u5bdf\u66f4\u52a0\u6e05\u6670\u3002\u51cf\u5c11\u8367\u5149\u66b4\u9732\uff0c\u63d0\u9ad8\u6d3b\u7ec6\u80de\u7684\u5b58\u6d3b\u7387\u3002<\/li>\n\n\n\n<li><strong>\u539a\u7ec4\u7ec7\u6210\u50cf<\/strong>\uff1a\u5728\u539a\u6807\u672c\u4e2d\u6539\u5584\u6e05\u6670\u5ea6\uff0c\u51cf\u5c11\u79bb\u7126\u5149\uff0c\u4ece\u800c\u6709\u52a9\u4e8e\u590d\u6742\u7ec4\u7ec7\u7684\u7814\u7a76\u3002<\/li>\n\n\n\n<li><strong>\u5171\u5b9a\u4f4d\u7814\u7a76<\/strong>\uff1a\u901a\u8fc7\u6700\u5c0f\u5316\u4fe1\u53f7\u4e32\u6270\uff0c\u5b9e\u73b0\u591a\u79cd\u8367\u5149\u6807\u8bb0\u7269\u66f4\u51c6\u786e\u7684\u91cd\u53e0\u5206\u6790\u3002<\/li>\n\n\n\n<li><strong>\u8d85\u5206\u8fa8\u7387\u6280\u672f<\/strong>\uff1a\u5c06\u53bb\u5377\u79ef\u4e0e\u7ed3\u6784\u5316\u7167\u660e\u663e\u5fae\u955c\uff08SIM\uff09\u7b49\u65b9\u6cd5\u7ed3\u5408\uff0c\u7a81\u7834\u4f20\u7edf\u5206\u8fa8\u7387\u6781\u9650\u3002<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u53bb\u5377\u79ef\u662f\u5982\u4f55\u5de5\u4f5c\u7684\">\u53bb\u5377\u79ef\u662f\u5982\u4f55\u5de5\u4f5c\u7684\uff1f<\/h2>\n\n\n\n<p>\u53bb\u5377\u79ef\u4f9d\u8d56\u4e8e\u590d\u6742\u7684\u56fe\u50cf\u7b97\u6cd5\uff0c\u5176\u6613\u7528\u6027\u901a\u5e38\u53d6\u51b3\u4e8e\u6240\u4f7f\u7528\u7684\u5de5\u5177\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u70b9\u6269\u6563\u51fd\u6570\">\u70b9\u6269\u6563\u51fd\u6570<\/h3>\n\n\n\n<p>\u5728\u663e\u5fae\u955c\u9886\u57df\uff0c<strong>\u70b9\u6269\u6563\u51fd\u6570\uff08PSF\uff09<\/strong>\u63cf\u8ff0\u4e86\u4e00\u4e2a\u70b9\u5149\u6e90\u2014\u2014\u5982\u8367\u5149\u5206\u5b50\u2014\u2014\u7531\u4e8e\u5149\u5b66\u7cfb\u7edf\u56fa\u6709\u7684\u884d\u5c04\u548c\u7f3a\u9677\uff0c\u5728\u56fe\u50cf\u4e2d\u5448\u73b0\u7684\u65b9\u5f0f\u3002\u5149\u4e0d\u4f1a\u5f62\u6210\u5b8c\u7f8e\u7684\u70b9\uff0c\u800c\u662f\u6269\u6563\u5f00\u6765\uff0c\u4ea7\u751f\u4e00\u79cd\u7279\u5f81\u6027\u6a21\u5f0f\uff0c\u53cd\u6620\u51fa\u7279\u5b9a\u663e\u5fae\u955c\u5bf9\u56fe\u50cf\u7684\u6a21\u7cca\u7a0b\u5ea6\u3002<\/p>\n\n\n\n<p>\u901a\u8fc7\u5bf9PSF\u8fdb\u884c\u5efa\u6a21\uff0c\u53bb\u5377\u79ef\u7b97\u6cd5\u53ef\u4ee5\u5c06\u79bb\u7126\u5149\u91cd\u65b0\u5206\u914d\u5230\u5176\u539f\u59cb\u4f4d\u7f6e\uff0c\u4ece\u800c\u63d0\u5347\u56fe\u50cf\u6e05\u6670\u5ea6\u548c\u5206\u8fa8\u7387\u3002\u8fd9\u4e00\u8fc7\u7a0b\u5728\u8367\u5149\u663e\u5fae\u955c\u4e2d\u5c24\u4e3a\u91cd\u8981\uff0c\u56e0\u4e3a\u79bb\u7126\u5149\u4f1a\u4e25\u91cd\u906e\u853d\u7ec6\u5fae\u7ed3\u6784\u7ec6\u8282\u3002<\/p>\n\n\n\n<p>PSF\u53ef\u4ee5\u901a\u8fc7\u7406\u8bba\u65b9\u6cd5\uff08\u57fa\u4e8e\u663e\u5fae\u955c\u7684\u5149\u5b66\u53c2\u6570\uff09\u6216\u7ecf\u9a8c\u65b9\u6cd5\uff08\u62cd\u6444\u4e9a\u5206\u8fa8\u7387\u73e0\u5b50\u5e76\u5206\u6790\u5176\u5149\u6269\u6563\u60c5\u51b5\uff09\u83b7\u5f97\u3002\u51c6\u786e\u4e86\u89e3PSF\u80fd\u591f\u5b9e\u73b0\u66f4\u6709\u6548\u7684\u53bb\u5377\u79ef\u5904\u7406\uff0c\u4f7f\u56fe\u50cf\u66f4\u52a0\u9510\u5229\u3001\u7ec6\u8282\u66f4\u4e30\u5bcc\uff0c\u66f4\u597d\u5730\u53cd\u6620\u6837\u54c1\u7684\u5b9e\u9645\u7ed3\u6784\u3002<\/p>\n\n\n\n<p>EVOS Analysis\u53bb\u5377\u79ef\u5de5\u5177\u91c7\u7528\u81ea\u9002\u5e94PSF\uff0c\u8fd9\u662f\u4e00\u79cd\u53d7\u7ea6\u675f\u8fed\u4ee3\u8ba1\u7b97\u7b97\u6cd5\u3002\u4e0e\u6570\u5b57\u96fe\u5316\u51cf\u5f31\u65b9\u6cd5\u4e0d\u540c\uff0c\u81ea\u9002\u5e94PSF\u901a\u8fc7\u5c06\u6563\u5c04\u5149\u91cd\u65b0\u5206\u914d\u5230\u5176\u539f\u59cb\u4f4d\u7f6e\u6765\u6062\u590d\u56fe\u50cf\uff0c\u51cf\u5c11\u80cc\u666f\u8367\u5149\u5e76\u589e\u5f3a\u8367\u5149\u4fe1\u53f7\u3002\u8fd9\u9879\u6280\u672f\u80fd\u591f\u89e3\u6790\u539f\u59cb\u56fe\u50cf\u4e2d\u5fae\u5f31\u3001\u6a21\u7cca\u7684\u7ec6\u8282\u3002\u5728\u9002\u5f53\u63a7\u5236\u6761\u4ef6\u4e0b\uff0c\u7ecf\u81ea\u9002\u5e94PSF\u53bb\u5377\u79ef\u5904\u7406\u540e\u7684\u56fe\u50cf\u53ef\u7528\u4e8e\u5b9a\u91cf\u8367\u5149\u6d4b\u91cf\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u53bb\u5377\u79ef\u7b97\u6cd5\">\u53bb\u5377\u79ef\u7b97\u6cd5<\/h3>\n\n\n\n<p>\u5728\u663e\u5fae\u955c\u9886\u57df\uff0c\u53bb\u5377\u79ef\u7b97\u6cd5\u5bf9\u4e8e\u63d0\u5347\u56fe\u50cf\u6e05\u6670\u5ea6\u81f3\u5173\u91cd\u8981\uff0c\u5b83\u4eec\u901a\u8fc7\u7f13\u89e3\u7531\u5149\u5b66\u7cfb\u7edf\u70b9\u6269\u6563\u51fd\u6570\uff08PSF\uff09\u5f15\u5165\u7684\u6a21\u7cca\u6548\u5e94\u6765\u5b9e\u73b0\u8fd9\u4e00\u76ee\u6807\u3002\u8fd9\u4e9b\u7b97\u6cd5\u4e3b\u8981\u5206\u4e3a\u4e24\u7c7b\uff1a\u9006\u6ee4\u6ce2\u65b9\u6cd5\u548c\u8fed\u4ee3\u65b9\u6cd5\u3002<\/p>\n\n\n\n<p><strong>\u8fed\u4ee3\u7b97\u6cd5<\/strong>\u5c06\u53bb\u5377\u79ef\u89c6\u4e3a\u4e00\u4e2a\u9010\u6b65\u7cbe\u7ec6\u5316\u7684\u8fc7\u7a0b\u3002\u4ed6\u4eec\u4ece\u771f\u5b9e\u56fe\u50cf\u7684\u521d\u59cb\u4f30\u8ba1\u5f00\u59cb\uff0c\u5e76\u4e0d\u65ad\u66f4\u65b0\u8be5\u4f30\u8ba1\uff0c\u4ee5\u6700\u5c0f\u5316\u89c2\u6d4b\u56fe\u50cf\u4e0e\u4f30\u8ba1\u503c\u548c\u70b9\u6269\u6563\u51fd\u6570\uff08PSF\uff09\u5377\u79ef\u4e4b\u95f4\u7684\u5dee\u5f02\u3002\u8fd9\u79cd\u65b9\u6cd5\u5728\u5904\u7406\u566a\u58f0\u548c\u590d\u6742\u6a21\u7cca\u65b9\u9762\u7279\u522b\u6709\u6548\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>Richardson\u2013Lucy\uff08RL\uff09\u53bb\u5377\u79ef\uff1a<\/strong>\u5047\u8bbe\u5b58\u5728\u6cca\u677e\u566a\u58f0\uff0c\u8fd9\u5728\u5149\u5b50\u53d7\u9650\u6210\u50cf\u4e2d\u5f88\u5e38\u89c1\u3002\u5b83\u9010\u6b65\u4f18\u5316\u56fe\u50cf\u4f30\u8ba1\uff0c\u5c24\u5176\u5728\u4f4e\u5149\u6761\u4ef6\u4e0b\u80fd\u63d0\u4f9b\u9ad8\u8d28\u91cf\u7684\u4fee\u590d\u6548\u679c\u3002<\/li>\n\n\n\n<li><strong>\u6700\u5927\u4f3c\u7136\u4f30\u8ba1\uff08MLE\uff09\uff1a<\/strong>\u4e00\u79cd\u7edf\u8ba1\u65b9\u6cd5\uff0c\u6839\u636e\u89c2\u6d4b\u6570\u636e\u548c\u566a\u58f0\u7279\u6027\uff0c\u4f30\u7b97\u6700\u53ef\u80fd\u7684\u539f\u59cb\u56fe\u50cf\u3002\u5b83\u5177\u6709\u5f88\u5f3a\u7684\u9c81\u68d2\u6027\uff0c\u4f46\u8ba1\u7b97\u91cf\u5927\uff0c\u5e38\u7528\u4e8e\u9ad8\u7cbe\u5ea6\u5e94\u7528\u573a\u666f\u3002<\/li>\n\n\n\n<li><strong>Landweber\u8fed\u4ee3\u6cd5\uff1a<\/strong>\u4e00\u79cd\u7b80\u5355\u7684\u8fed\u4ee3\u65b9\u6cd5\uff0c\u901a\u8fc7\u6cbf\u8bef\u5dee\u68af\u5ea6\u76f8\u53cd\u65b9\u5411\u66f4\u65b0\u56fe\u50cf\u4f30\u8ba1\u3002\u867d\u7136\u64cd\u4f5c\u7b80\u5355\uff0c\u4f46\u6536\u655b\u901f\u5ea6\u8f83\u6162\uff0c\u901a\u5e38\u9700\u8981\u5927\u91cf\u8fed\u4ee3\u6b21\u6570\u3002<\/li>\n\n\n\n<li><strong>\u5168\u53d8\u5206\uff08TV\uff09\u6b63\u5219\u5316\uff1a<\/strong>\u5f15\u5165\u6b63\u5219\u9879\u4ee5\u5728\u964d\u566a\u540c\u65f6\u4fdd\u7559\u8fb9\u7f18\u4fe1\u606f\u3002\u5bf9\u4e8e\u5177\u6709\u9510\u5229\u7279\u5f81\u7684\u56fe\u50cf\u975e\u5e38\u6709\u76ca\uff0c\u5728\u53bb\u5377\u79ef\u8fc7\u7a0b\u4e2d\u80fd\u591f\u4fdd\u6301\u7ed3\u6784\u5b8c\u6574\u6027\u3002<\/li>\n<\/ul>\n\n\n\n<p><strong>\u9006\u6ee4\u6ce2\u65b9\u6cd5<\/strong>\u65e8\u5728\u901a\u8fc7\u5bf9\u70b9\u6269\u6563\u51fd\u6570\uff08PSF\uff09\u5e94\u7528\u9006\u64cd\u4f5c\uff0c\u76f4\u63a5\u9006\u8f6c\u6a21\u7cca\u6548\u5e94\u3002\u8fd9\u4e9b\u65b9\u6cd5\u901a\u5e38\u901f\u5ea6\u8f83\u5feb\uff0c\u4f46\u5bf9\u566a\u58f0\u66f4\u4e3a\u654f\u611f\u3002<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><strong>\u6734\u7d20\u9006\u6ee4\u6ce2\uff1a<\/strong>\u76f4\u63a5\u5c06\u89c2\u6d4b\u56fe\u50cf\u7684\u5085\u91cc\u53f6\u53d8\u6362\u9664\u4ee5PSF\u7684\u5085\u91cc\u53f6\u53d8\u6362\u3002\u867d\u7136\u65b9\u6cd5\u7b80\u5355\uff0c\u4f46\u5bf9\u566a\u58f0\u6781\u4e3a\u654f\u611f\u3002<\/li>\n\n\n\n<li><strong>\u7ef4\u7eb3\u53bb\u5377\u79ef\uff1a<\/strong>\u662f\u5728\u9006\u6ee4\u6ce2\u57fa\u7840\u4e0a\u6269\u5c55\u7684\u65b9\u6cd5\uff0c\u7ed3\u5408\u4e86\u566a\u58f0\u4e0e\u4fe1\u53f7\u7684\u6bd4\u7387\uff0c\u65e8\u5728\u6700\u5c0f\u5316\u4f30\u8ba1\u56fe\u50cf\u4e0e\u771f\u5b9e\u56fe\u50cf\u4e4b\u95f4\u7684\u5747\u65b9\u8bef\u5dee\u3002\u5b83\u53ef\u4ee5\u5728\u53bb\u6a21\u7cca\u548c\u6291\u5236\u566a\u58f0\u4e4b\u95f4\u5b9e\u73b0\u5e73\u8861\u3002<\/li>\n\n\n\n<li><strong>Tikhonov\u6b63\u5219\u5316\uff1a<\/strong>\u901a\u8fc7\u6dfb\u52a0\u6b63\u5219\u5316\u9879\u6765\u7a33\u5b9a\u53cd\u6f14\u8fc7\u7a0b\uff0c\u4ece\u800c\u51cf\u5c11\u566a\u58f0\u5f71\u54cd\uff0c\u63d0\u9ad8\u53bb\u5377\u79ef\u7684\u9c81\u68d2\u6027\u3002<\/li>\n\n\n\n<li><strong>\u76f2\u53bb\u5377\u79ef\uff1a<\/strong>\u5f53PSF\u672a\u77e5\u65f6\u4f7f\u7528\uff0c\u8be5\u65b9\u6cd5\u540c\u65f6\u4f30\u8ba1PSF\u548c\u53bb\u6a21\u7cca\u540e\u7684\u56fe\u50cf\u3002\u5f53\u65e0\u6cd5\u5b9e\u9645\u6d4b\u91cfPSF\u65f6\uff0c\u8fd9\u79cd\u65b9\u6cd5\u5c24\u4e3a\u6709\u7528\uff0c\u4f46\u9700\u8981\u8c28\u614e\u5b9e\u73b0\u4ee5\u907f\u514d\u4ea7\u751f\u4f2a\u5f71\u3002<\/li>\n\n\n\n<li><strong>AI\u9a71\u52a8\u7b97\u6cd5<\/strong>\u6700\u8fd1\u7684\u8fdb\u5c55\u5305\u62ec\u4f7f\u7528\u6df1\u5ea6\u5b66\u4e60\u6a21\u578b\uff0c\u5982\u5377\u79ef\u795e\u7ecf\u7f51\u7edc\uff08CNN\uff09\uff0c\u6765\u8fdb\u884c\u53cd\u5377\u79ef\u3002\u8fd9\u4e9b\u6a21\u578b\u80fd\u591f\u5b66\u4e60\u4ece\u6a21\u7cca\u56fe\u50cf\u5230\u6e05\u6670\u56fe\u50cf\u7684\u590d\u6742\u6620\u5c04\uff0c\u5728\u67d0\u4e9b\u573a\u666f\u4e0b\u63d0\u4f9b\u4e86\u66f4\u597d\u7684\u6027\u80fd\u3002<\/li>\n<\/ul>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-2d\u56fe\u50cf\u53bb\u5377\u79ef\u76845\u4e2a\u793a\u4f8b-\u5904\u7406\u524d\u4e0e\u5904\u7406\u540e\u5bf9\u6bd4\">2D\u56fe\u50cf\u53bb\u5377\u79ef\u76845\u4e2a\u793a\u4f8b\uff0c\u5904\u7406\u524d\u4e0e\u5904\u7406\u540e\u5bf9\u6bd4<\/h2>\n\n\n\n<p>\u8fd9\u4e9b\u793a\u4f8b\u5c55\u793a\u4e86\u53cd\u5377\u79ef\u5728\u5206\u6790\u548c\u53d1\u8868\u76ee\u7684\u4e0a\u6240\u5e26\u6765\u7684\u5dee\u5f02\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-1-\u5c0f\u9f20\u80be\u810f\">1. \u5c0f\u9f20\u80be\u810f<\/h3>\n\n\n\n<figure class=\"wp-block-jetpack-image-compare\"><div class=\"juxtapose\" data-mode=\"vertical\"><img loading=\"lazy\" decoding=\"async\" id=\"4831\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/1a-2.jpg\" alt=\"\" width=\"500\" height=\"500\" class=\"image-compare__image-before\" \/><img loading=\"lazy\" decoding=\"async\" id=\"4832\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/1b-1-1.jpg\" alt=\"\" width=\"500\" height=\"500\" class=\"image-compare__image-after\" \/><\/div><figcaption>FluoCells\u2122\u9884\u5236\u8f7d\u73bb\u7247#3\uff08\u5c0f\u9f20\u80be\u810f\u5207\u7247\uff0c\u6807\u8bb0\u6709Alexa Fluor\u2122 488 WGA\u3001Alexa Fluor\u2122 568\u9b3c\u7b14\u73af\u80bd\u548cDAPI\uff09\uff0c\u4f7f\u7528EVOS M7000\u6210\u50cf\u7cfb\u7edf\u4ee54X\u500d\u7387\u62cd\u6444\u3002<\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-2-\u5c0f\u9f20\u7ed3\u80a0\">2. \u5c0f\u9f20\u7ed3\u80a0<div class=\"glfh_linkContainer\"><a title=\"Copy link to clipboard\" href=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-admin\/post.php?post=4747&amp;action=edit#block-fe0d891f-e10f-43f6-97cd-7803f86baf73\">\ud83d\udd17<\/a><\/div><\/h3>\n\n\n\n<figure class=\"wp-block-jetpack-image-compare\"><div class=\"juxtapose\" data-mode=\"vertical\"><img loading=\"lazy\" decoding=\"async\" id=\"4833\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/2a-1.jpg\" alt=\"\" width=\"500\" height=\"500\" class=\"image-compare__image-before\" \/><img loading=\"lazy\" decoding=\"async\" id=\"4834\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/2b-1.jpg\" alt=\"\" width=\"500\" height=\"500\" class=\"image-compare__image-after\" \/><\/div><figcaption>Mouse colon labeled with Alexa Fluor\u2122 488 conjugated CD4 primary antibody and DAPI, mounted in ProLong antifade mountant, and imaged on EVOS M7000 Imaging System at 60X magnification.\u00a0<\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-3-hela\u7ec6\u80de\">3. HeLa\u7ec6\u80de<\/h3>\n\n\n\n<figure class=\"wp-block-jetpack-image-compare\"><div class=\"juxtapose\" data-mode=\"vertical\"><img loading=\"lazy\" decoding=\"async\" id=\"4835\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/3a-1.jpg\" alt=\"\" width=\"500\" height=\"500\" class=\"image-compare__image-before\" \/><img loading=\"lazy\" decoding=\"async\" id=\"4836\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/3b.jpg\" alt=\"\" width=\"500\" height=\"500\" class=\"image-compare__image-after\" \/><\/div><figcaption>Hela cells (PFA fixed) labeled with DAPI, anti-Coilin Antibody, Beta-Tubulin Monoclonal Antibody, and Ki-67 Recombinant Rat Monoclonal Antibody. Imaged on EVOS M7000 Imaging System at 20X magnification using ProLong mounting media.\u00a0<\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-4-bpae\u7ec6\u80de\">4. BPAE\u7ec6\u80de<\/h3>\n\n\n\n<figure class=\"wp-block-jetpack-image-compare\"><div class=\"juxtapose\" data-mode=\"vertical\"><img loading=\"lazy\" decoding=\"async\" id=\"4837\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/4a.png\" alt=\"\" width=\"500\" height=\"374\" class=\"image-compare__image-before\" \/><img loading=\"lazy\" decoding=\"async\" id=\"4838\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/4b.jpg\" alt=\"\" width=\"500\" height=\"374\" class=\"image-compare__image-after\" \/><\/div><figcaption>FluoCells\u2122 Prepared Slide #1 (BPAE cells with MitoTracker\u2122 Red CMXRos, Alexa Fluor\u2122 488 Phalloidin, and DAPI), imaged on EVOS M7000 Imaging System at 40X magnification.<\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-5-u2os\u7ec6\u80de\">5. U2OS\u7ec6\u80de<\/h3>\n\n\n\n<figure class=\"wp-block-jetpack-image-compare\"><div class=\"juxtapose\" data-mode=\"vertical\"><img loading=\"lazy\" decoding=\"async\" id=\"4839\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/6a.jpg\" alt=\"\" width=\"500\" height=\"375\" class=\"image-compare__image-before\" \/><img loading=\"lazy\" decoding=\"async\" id=\"4840\" src=\"https:\/\/admin.acceleratingscience.com\/life-in-the-lab\/wp-content\/uploads\/sites\/10\/2025\/07\/6b.jpg\" alt=\"\" width=\"500\" height=\"376\" class=\"image-compare__image-after\" \/><\/div><figcaption>U2OS cells plated in Nunc\u2122 Glass Bottom Dishes (Cat. No. 150682) and labeled with Hoechst 33342, trihydrochloride trihydrate, 10 mg\/mL (Cat. No. H3570, nucleus-blue), Alexa Fluor\u2122 488 phalloidin (Cat. No. A12379, actin-green), and alpha Tubulin Monoclonal Antibody (236-10501) (Cat. No. A11126) with Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor\u2122 647 (Cat. No. A21235, tubulin-purple). Imaged on EVOS M7000 Imaging System (Cat. No. AMF7000) with EVOS 100X Oil Objective, fluorite, coverslip-corrected (Cat. No. AMEP4696).\u00a0<\/figcaption><\/figure>\n\n\n\n<div style=\"height:25px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u66f4\u591a\u53bb\u5377\u79ef\u5b66\u4e60\u8d44\u6e90\">\u66f4\u591a\u53bb\u5377\u79ef\u5b66\u4e60\u8d44\u6e90<\/h2>\n\n\n\n<p>\u60a8\u53ef\u4ee5\u5728 thermofisher.com \u4e0a\u4e86\u89e3\u66f4\u591a\u5173\u4e8e\u6210\u50cf\u3001\u53bb\u5377\u79ef\u7b49\u76f8\u5173\u4fe1\u606f\uff1a<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/evos-cell-imaging-systems\/sample-data.html#video-gallery\" target=\"_blank\" rel=\"noreferrer noopener\">EVOS \u56fe\u50cf\u5e93\u4e0e\u6837\u672c\u6570\u636e<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/evos-cell-imaging-systems\/sample-data.html#sample-applications\" target=\"_blank\" rel=\"noreferrer noopener\">\u6210\u50cf\u5e94\u7528\u8bf4\u660e\u6587\u732e\u5e93<\/a> \u2013 \u521b\u4f24\u6108\u5408\u3001\u7ec6\u80de\u6d3b\u529b\u3001\u541e\u566c\u4f5c\u7528\u3001CRISPR \u6572\u9664\u3001\u7c7b\u7403\u4f53\u5206\u6790\u7b49\u66f4\u591a\u5e94\u7528<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/stain-it\/#!\/\" target=\"_blank\" rel=\"noreferrer noopener\">Stain-iT \u7ec6\u80de\u67d3\u8272\u6a21\u62df\u5668<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/cell-imaging-systems\/evos-citations.html\" target=\"_blank\" rel=\"noreferrer noopener\">EVOS \u7ec6\u80de\u6210\u50cf\u7cfb\u7edf\u5f15\u7528\u6587\u732e<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/evos-cell-imaging-systems\/resources.html#documents-and-downloads\" target=\"_blank\" rel=\"noreferrer noopener\">\u5ba2\u6237\u6848\u4f8b\uff1a\u4f7f\u7528EVOS\u6210\u50cf\u7cfb\u7edf<\/a><\/li>\n<\/ul>\n\n\n\n<div style=\"height:15px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-width wp-block-button__width-75 is-style-fill\"><a class=\"wp-block-button__link has-white-color has-text-color has-background has-link-color has-medium-font-size has-text-align-center has-custom-font-size wp-element-button\" href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/life-science\/cell-analysis\/cellular-imaging\/evos-cell-imaging-systems\/models\/evos-m7000.html\" style=\"border-radius:0px;background-color:#ee3134\" target=\"_blank\" rel=\"noreferrer noopener\"><strong><strong>\u4f7f\u7528EVOS 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