{"id":912,"date":"2026-03-12T03:29:12","date_gmt":"2026-03-12T03:29:12","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/?p=912"},"modified":"2026-05-28T06:36:02","modified_gmt":"2026-05-28T06:36:02","slug":"flow-cytometry-compensation","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/accelerating-science-in-china\/flow-cytometry-compensation\/","title":{"rendered":"\u6d41\u5f0f\u8865\u507f\uff08Compensation\uff09\u5e38\u89c1\u95ee\u9898\u4e0e\u8bbe\u7f6e\u6307\u5357"},"content":{"rendered":"\n<figure class=\"wp-block-image size-large\"><a href=\"https:\/\/www.thermofisher.cn\/cn\/zh\/home\/communities-social\/socialhub\/blog-subscribe.html?icid=cn-blog-subscribe\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"176\" src=\"http:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1024x176.jpg\" alt=\"\" class=\"wp-image-2271\" srcset=\"https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1024x176.jpg 1024w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-300x52.jpg 300w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-768x132.jpg 768w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-1536x264.jpg 1536w, https:\/\/admin.acceleratingscience.com\/accelerating-science-in-china\/wp-content\/uploads\/sites\/28\/2026\/05\/779-\u00d7-134-3-2048x352.jpg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/a><\/figure>\n\n\n\n<div 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data-kb-block=\"kb-adv-heading912_d4cd28-e7\"><strong>\u54ea\u4e9b\u67d3\u6599\u4e4b\u95f4\u7684\u8865\u507f\u503c\u6bd4\u8f83\u5927\uff0c\u4e0d\u5efa\u8bae\u540c\u65f6\u4f7f\u7528\uff1f<\/strong><\/h2>\n\n\n\n<p>\u6d41\u5f0f\u5b9e\u9a8c\u4e2d\u8367\u5149\u67d3\u6599\u7684\u5149\u8c31\u91cd\u53e0\u5f80\u5f80\u65e0\u6cd5\u907f\u514d\uff0c\u5c24\u5176\u662f\u5bf9\u4e8e\u591a\u8272\u6d41\u5f0f\u5b9e\u9a8c\uff0c\u5f88\u591a\u5149\u8c31\u91cd\u53e0\u9020\u6210\u7684\u5f71\u54cd\u53ef\u4ee5\u901a\u8fc7\u4f18\u5316\u914d\u8272\u65b9\u6848\u548c\u8865\u507f\u6765\u51cf\u5c0f\u3002\u4f46\u662f\uff0c\u786e\u5b9e\u6709\u4e9b\u67d3\u6599\uff0c\u7531\u4e8e\u5149\u8c31\u91cd\u53e0\u8fc7\u4e8e\u5f3a\uff0c\u8981\u7279\u522b\u6ce8\u610f\u8fd9\u4e9b\u67d3\u6599\u540c\u65f6\u4f7f\u7528\u65f6\u7684\u8865\u507f\u8c03\u8282\u3002\u6bd4\u5982\uff0cAPC\u4e0ePE-Cy5\uff1bBV711\uff0cAF700\uff0cPE-Cy5.5\uff0cPerCP-Cy5.5\uff1b&nbsp;AMCyan\u548cFITC\u7b49\u3002\u8fd8\u6709\u5f88\u591a\u67d3\u6599\uff0c\u5149\u8c31\u6781\u5176\u63a5\u8fd1\uff0c\u5728<strong>\u4f20\u7edf\u6d41\u5f0f\u7ec6\u80de\u4eea<\/strong>\u4e0a\u662f\u540c\u4e00\u4e2a\u901a\u9053\u68c0\u6d4b\u7684\uff0c\u8fd9\u4e9b\u67d3\u6599\u662f\u4e0d\u80fd\u540c\u65f6\u4f7f\u7528\u7684\u3002\u6bd4\u5982\uff0cFITC\u548cAF488\uff1bAPC\uff0c&nbsp;AF647\u548ceFluor660\uff1beFluor450\uff0cBV421\u548cSuper 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mark.kt-highlight{font-style:normal;color:#f76a0c;-webkit-box-decoration-break:clone;box-decoration-break:clone;padding-top:0px;padding-right:0px;padding-bottom:0px;padding-left:0px;}.wp-block-kadence-advancedheading.kt-adv-heading912_a2931c-a0 img.kb-inline-image, .wp-block-kadence-advancedheading.kt-adv-heading912_a2931c-a0[data-kb-block=\"kb-adv-heading912_a2931c-a0\"] img.kb-inline-image{width:150px;vertical-align:baseline;}<\/style>\n<h2 class=\"kt-adv-heading912_a2931c-a0 wp-block-kadence-advancedheading\" data-kb-block=\"kb-adv-heading912_a2931c-a0\">\u5149\u8c31\u6d41\u5f0f\u7ec6\u80de\u4eea<strong>\u662f\u4e0d\u9700\u8981\u8fdb\u884c\u8865\u507f\u8c03\u8282\u5417\uff1f<\/strong><\/h2>\n\n\n\n<p>\u8fd9\u91cc\u8bb2\u7684\u8865\u507f\u5185\u5bb9\u90fd\u662f\u9488\u5bf9\u4e8e\u4f20\u7edf\u6d41\u5f0f\u7ec6\u80de\u4eea\u7684\u3002\u5bf9\u4e8e\u5149\u8c31\u6d41\u5f0f\u6765\u8bf4\uff0c\u662f\u4e0d\u5b58\u5728\u8865\u507f\u7684\u8fd9\u4e2a\u6982\u5ff5\u7684\uff0c\u6240\u4ee5\u5149\u8c31\u6d41\u5f0f\u4e0d\u9700\u8981\u8865\u507f\u8fd9\u79cd\u8bf4\u6cd5\u4e5f\u7b97\u662f\u6b63\u786e\u7684\u3002\u4f46\u662f\uff0c\u8fd9\u5e76\u4e0d\u610f\u5473\u7740\u5149\u8c31\u6d41\u5f0f\u4f1a\u66f4\u52a0\u7684\u7b80\u5355\uff0c\u56e0\u4e3a\u5149\u8c31\u6d41\u5f0f\u662f\u9700\u8981\u901a\u8fc7\u5149\u8c31\u89e3\u6790\uff08Spectral Unmixing\uff09\u6765\u8fdb\u884c\u6570\u636e\u62c6\u89e3\u7684\uff0c\u800c\u5149\u8c31\u89e3\u6790\u4e5f\u9700\u8981\u5355\u67d3\u5bf9\u7167\u3002<strong>UltraComp eBeads Spectral Unmixing Beads (\u8d27\u53f7:&nbsp;<a href=\"https:\/\/www.thermofisher.cn\/order\/catalog\/product\/U20250?icid=blog\">U20250<\/a>)&nbsp;\u7ecf\u8fc7\u4f18\u5316\u4e8e\u5149\u8c31\u6d41\u5f0f\u7ec6\u80de\u672f\uff0c\u901a\u8fc7\u4f9b\u7cbe\u786e\u7684\u5355\u5149\u8c31\u89e3\u6790\u5bf9\u7167\uff0c\u589e\u5f3a\u5149\u8c31\u6d41\u5f0f\u7ec6\u80de\u672f\u7684\u68c0\u6d4b\u6027\u80fd\u3002<\/strong><\/p>\n\n\n\n<p><strong>\u6d41\u5f0f\u7ec6\u80de\u672f<\/strong>\u662f\u91cd\u8981\u7684\u7ec6\u80de\u5206\u6790\u65b9\u6cd5\u5b66\uff0c<strong>\u8d5b\u9ed8\u98de\u4e16\u5c14\u79d1\u6280<\/strong>\u7684Gibco\u3001Invitrogen\u548cThermo Scientific\u4ea7\u54c1\u54c1\u724c\u53ef\u63d0\u4f9b\u4ece\u6d41\u5f0f\u4eea\u5668\u3001\u6297\u4f53\u8bd5\u5242\u5230\u4e30\u5bcc\u8d44\u6e90\u7684<strong>\u6d41\u5f0f\u6574\u4f53\u89e3\u51b3\u65b9\u6848<\/strong>\u3002\u5176\u4e2d<strong>Invitrogen&nbsp;<\/strong>eBioscience\u966a\u4f34\u7740\u6bcf\u4e00\u4ee3\u6d41\u5f0f\u79d1\u5b66\u5bb6\u548c\u6280\u672f\u4eba\u5458\u83b7\u5f97\u6210\u679c\u3001\u5171\u540c\u6210\u957f\uff0c\u8fd9\u4e9b\u5e74\u6765\u6211\u4eec\u4e00\u8d77\u89c1\u8bc1\u4ee5Anti-FoxP3\u4e3a\u4ee3\u8868\u7684\u6d41\u5f0f\u6297\u4f53\u6df1\u53d7\u559c\u7231\u3001\u66f4\u591a\u79cd\u7c7b\u7684\u6d41\u5f0f\u8367\u5149\u67d3\u6599\u6b23\u7136\u4e0a\u5e02\u4ee5\u53ca\u8d5b\u9ed8\u98de\u79d1\u5b66\u5bb6\u4eec\u7684\u5341\u5e74\u5982\u4e00\u65e5\u6280\u672f\u966a\u4f34\uff0c\u73b0\u5728\u6709\u7740\u66f4\u4e30\u5bcc\u7684\u4ea7\u54c1\u548c\u5de5\u5177\uff0c\u6ee1\u8db3\u9ad8\u6821\u7814\u7a76\u9662\u3001\u751f\u7269\u6280\u672f\u516c\u53f8\u3001\u836f\u4f01\u7b49\u4ece\u65e9\u671f\u57fa\u7840\u7814\u7a76\u5230\u4e34\u5e8a\u8f6c\u5316\u548c\u751f\u4ea7\u7b49\u4e0d\u540c\u9636\u6bb5\u7684\u9700\u6c42\uff0c\u52a0\u901f\u751f\u547d\u79d1\u5b66\u884c\u4e1a\u7684\u8fdb\u7a0b\u53d1\u5c55\u3002<\/p>\n\n\n<script 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