{"id":18374,"date":"2025-02-19T00:18:16","date_gmt":"2025-02-19T00:18:16","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/analyteguru\/?p=18374"},"modified":"2026-09-11T20:37:38","modified_gmt":"2026-09-11T20:37:38","slug":"automated-lc-ms-sample-cleanup-using-turbulent-flow-chromatography","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/analyteguru\/automated-lc-ms-sample-cleanup-using-turbulent-flow-chromatography\/","title":{"rendered":"Automated LC-MS Sample Cleanup Using Turbulent Flow Chromatography"},"content":{"rendered":"\n<h3 class=\"wp-block-heading\" id=\"h-too-much-interfering-matrix-discover-how-to-streamline-your-daily-lc-ms-analysis-with-turbulent-flow-chromatography\">Too much interfering matrix? Discover how to streamline your daily LC-MS analysis with turbulent flow chromatography.<\/h3>\n\n\n\n<p>Sample preparation is a critical step in liquid chromatography-mass spectrometry (LC-MS) analysis to achieve quantitative accuracy and precision. Directly injecting crude samples into your instrument without prior cleanup to remove interfering compounds can clog the flow path, damage your detector, and negatively impact your analyses through a \u201cmatrix effect.\u201d<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-style-default\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"512\" src=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/Turboflow-blog-main-image-1024x512.jpeg\" alt=\"Too much interfering matrix? Discover how to streamline your daily LC-MS analysis with turbulent flow chromatography.\" class=\"wp-image-18375\" srcset=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/Turboflow-blog-main-image-1024x512.jpeg 1024w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/Turboflow-blog-main-image-300x150.jpeg 300w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/Turboflow-blog-main-image-768x384.jpeg 768w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/Turboflow-blog-main-image.jpeg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>Common approaches to clean up samples and suppress the matrix effect include simple dilution and centrifugation to extensive extraction techniques. The common scope of all these procedures is to minimize the possible contamination of your LC-MS system but, more importantly, to reduce to a minimum the interference of unrelated sample components on the detection and quantification of your analytes of interest.<\/p>\n\n\n\n<p>A point to note is the preparation method you choose often depends on the complexity of your sample. For instance, samples for clinical research applications or toxicology tend to have high levels of interfering matrix and require a more sophisticated clean-up approach.&nbsp; Let\u2019s dive into what a matrix effect is, the impacts on your LC-MS data, and an effective method called turbulent flow chromatography you can use to clean complex samples.<sup>1<\/sup><\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-what-exactly-is-matrix-effect\"><strong>What exactly is matrix effect?<\/strong><\/h3>\n\n\n\n<p>Any chemical entity in your sample capable of coeluting or interacting with analytes of interest can generate matrix effect, ultimately impacting the integrity of your data.<\/p>\n\n\n\n<p>Interfering matrix compounds come from endogenous and exogenous sources, but biological samples like blood or serum, and food and environmental extracts inherently have higher levels of interfering compounds.<\/p>\n\n\n\n<p>Some examples of interfering matrix compounds include ion-suppressing salts, proteins, lipids, compounds capable of ion-pairing interactions, and polymers.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-how-does-matrix-effect-impact-your-lc-ms-data\"><strong>How does matrix effect impact your LC-MS data?<\/strong><\/h3>\n\n\n\n<p>Because matrix compounds interfere with the detection and quantitation of your target analytes, the effects ultimately lead to an under- or overestimation in analyte quantification.<\/p>\n\n\n\n<p>In the case of LC-MS, the matrix effect triggers ion suppression<sup>2<\/sup>, which happens when compounds from the sample matrix inhibit charge transfer from the ion source to your target analytes &#8211; a prime example is electrospray ionization, where the detection relies on efficient charge transfer to analytes.<\/p>\n\n\n\n<p>Analytes and matrix components will naturally compete for the available charge from the MS ion source. And when compounds of your sample matrix are present in higher concentrations than your analytes, these unwanted molecules can capture more of the charge, leaving fewer ions available for charge transfer to your analytes.<\/p>\n\n\n\n<p>Hence suppression of charge transfer to your analytes leads to poor accuracy and limits of detection and quantitation.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-sample-preparation-methods-to-remove-interfering-matrix\"><strong>Sample preparation methods to remove interfering matrix<\/strong><\/h3>\n\n\n\n<p>Using the example of ion suppression, you can see how eliminating the competition for charge transfer between matrix components and analytes through proper sample cleaning is essential to developing a method with quantitative accuracy.<\/p>\n\n\n\n<p>Solid-Phase Extraction (SPE) and Liquid-Liquid Extraction (LLE) are techniques used to clean samples before LC-MS analysis.<sup> 3,4<\/sup><\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>In SPE, a liquid sample is loaded onto a column packed with a solid adsorbent material. The analytes of interest are retained on the adsorbent material while unwanted matrix components are washed away. The retained analytes are then eluted using a solvent. This approach is typically developed on a specific panel of target analytes.<\/li>\n<\/ul>\n\n\n\n<ul class=\"wp-block-list\">\n<li>LLE is based on the differential solubility of analytes between two immiscible solvents; once a partition equilibrium is reached, the enriched solvent is separated and, usually, evaporated to dryness and reconstituted in a solvent compatible with LC-MS.<\/li>\n<\/ul>\n\n\n\n<p>Regardless of the <a href=\"\/content\/lifetech\/global\/en\/home\/industrial\/chromatography\/chromatography-learning-center\/chromatography-consumables\/sample-prep.html\">sample preparation<\/a> approach you decide to use, these manual procedures are imprecise, time-consuming, and labor-intensive\u2014even a quick and easy cleanup is subject to human errors like improper pipetting or sample mix-up.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-automated-online-sample-cleanup-for-efficient-sample-preparations\"><strong>Automated online sample cleanup for efficient sample preparations<\/strong><\/h3>\n\n\n\n<p>To overcome the bottlenecks and risks of manual approaches, automated sample cleanup is gaining popularity, thanks to much less human intervention combined with better speed and analytical reproducibility.<\/p>\n\n\n\n<p>Online SPE&nbsp;is an improvement over manual ones, where the extraction process integrates directly with your LC system. The sample is automatically injected onto an extraction cartridge, cleanup occurs, and your extracted sample transfers directly to the MS instrument (flow injection analysis) or to an analytical column for further separation and detection.<\/p>\n\n\n\n<p>Turbulent flow chromatography (TurboFlow) is a more advanced online sample cleanup approach combining the aspects of the chemical affinity of SPE with size exclusion chromatography (SEC).<sup>5<\/sup><\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-how-does-turbulent-flow-chromatography-work\"><strong>How does turbulent flow chromatography work?<\/strong><\/h3>\n\n\n\n<p>TurboFlow separates low molecular weight molecules from high molecular weight matrix components using a specialized sample extraction column on LC systems (<strong>Figure 2<\/strong>).<\/p>\n\n\n\n<p><strong>Here\u2019s a detailed look at how TurboFlow works<\/strong>:<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>Your sample is directly injected into a <a href=\"\/order\/catalog\/product\/CH-952434\">TurboFlow column<\/a> (typically large &gt; 50 \u00b5m particle size with macropores).<\/li>\n\n\n\n<li>The sample flows through the column at high linear velocities, creating turbulence to enhance the interaction between the sample components and the stationary phase.<\/li>\n\n\n\n<li>Low molecular weight molecules diffuse into the pores faster than high molecular weight components and quickly flush to waste. Only the small molecules with an affinity for the particle chemistry bind to the internal pore surfaces.<\/li>\n\n\n\n<li>A change in mobile phase composition elutes the small molecules bound by the TurboFlow column for further separation and detection.<\/li>\n<\/ol>\n\n\n\n<figure class=\"wp-block-image size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"512\" src=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/how-turboflow-chromtatography-works-1024x512.jpeg\" alt=\"Figure 1. Illustration showing how turbulent flow chromatography works.\" class=\"wp-image-18378\" srcset=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/how-turboflow-chromtatography-works-1024x512.jpeg 1024w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/how-turboflow-chromtatography-works-300x150.jpeg 300w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/how-turboflow-chromtatography-works-768x384.jpeg 768w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/how-turboflow-chromtatography-works.jpeg 1200w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\"><strong>Figure 1<\/strong>. Illustration showing how turbulent flow chromatography works.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-benefits-of-turbulent-flow-chromatography-for-sample-preparation-over-spe\"><strong>Benefits of turbulent flow chromatography for sample preparation over SPE<\/strong><\/h3>\n\n\n\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 60%\"><div class=\"wp-block-media-text__content\">\n<p>While both TurboFlow and online SPE streamline and automate the sample preparation process, TurboFlow has key differences and advantages to consider (Figure 2).<\/p>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"410\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/turboflow-versus-spe.jpeg\" alt=\"Figure 2. TurboFlow vs. SPE for sample cleanup\" class=\"wp-image-18380 size-full\" srcset=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/turboflow-versus-spe.jpeg 700w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/turboflow-versus-spe-300x176.jpeg 300w\" sizes=\"auto, (max-width: 700px) 100vw, 700px\" \/><\/figure><\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\"><strong>Figure 2<\/strong>. TurboFlow vs. SPE for sample cleanup<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li><strong>TurboFlow provides a cleaner sample compared to SPE<\/strong>\n<ul class=\"wp-block-list\">\n<li>The more efficient dual mode of action (chemical affinity plus SEC)<\/li>\n\n\n\n<li>The elution performed by a strong solvent plug potentially reduces the transfer of residual matrix components to your MS<\/li>\n<\/ul>\n<\/li>\n\n\n\n<li><strong>A cleaner sample means<\/strong>\n<ul class=\"wp-block-list\">\n<li>Reduced ion suppression, leading to increased sensitivity<\/li>\n\n\n\n<li>Less mass spectrometer contamination<\/li>\n\n\n\n<li>Extended analytical column lifespan<\/li>\n\n\n\n<li>Faster analytical gradients<br><br>Moreover, the opportunity to modulate the strength of the mobile phase reaching the analytical column allows for better analyte focusing on the head of the column, significantly improving your chromatography.<\/li>\n<\/ul>\n<\/li>\n<\/ol>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-streamline-your-daily-lc-ms-analysis-with-turboflow-technology\"><strong>Streamline your daily LC-MS analysis with TurboFlow technology<\/strong><\/h3>\n\n\n\n<div class=\"wp-block-media-text has-media-on-the-right is-stacked-on-mobile\" style=\"grid-template-columns:auto 60%\"><div class=\"wp-block-media-text__content\">\n<p>Automated sample cleanup using <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/brochures\/br-74120-lc-ms-transcend-uhplc-systems-br74120-en.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">online TurboFlow technology<\/a> is a rapid and efficient sample preparation approach that considerably improves method accuracy, reliability, and sensitivity.<br><br>Adopting this technology can even enhance the competitiveness of your laboratory by allowing faster results, lower costs per sample, and improved reproducibility and MS sensitivity.<\/p>\n<\/div><figure class=\"wp-block-media-text__media\"><img loading=\"lazy\" decoding=\"async\" width=\"700\" height=\"410\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/turbulent-flow-chromatography-system.jpg\" alt=\"Figure 3. Transcend TLX TurboFlow UHPLC System\" class=\"wp-image-18382 size-full\" srcset=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/turbulent-flow-chromatography-system.jpg 700w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2025\/02\/turbulent-flow-chromatography-system-300x176.jpg 300w\" sizes=\"auto, (max-width: 700px) 100vw, 700px\" \/><\/figure><\/div>\n\n\n\n<p class=\"has-text-align-center has-small-font-size\"><strong>Figure 3<\/strong>. Transcend TLX TurboFlow UHPLC System<\/p>\n\n\n\n<p><a href=\"\/content\/lifetech\/global\/en\/home\/industrial\/chromatography\/liquid-chromatography-lc\/hplc-uhplc-systems\/transcend-turboflow-tlx-systems.html\">Learn how TurboFlow can ease your lab\u2019s daily LC-MS analysis of complex matrices today<\/a>.<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-literature-references\"><strong>Literature references<\/strong><\/h3>\n\n\n\n<ol class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/abs\/pii\/S0021967321005689\" target=\"_blank\" rel=\"noreferrer noopener\">Recent developments in sample preparation techniques combined with high-performance liquid chromatography: A critical review<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/34546235\/\" target=\"_blank\" rel=\"noreferrer noopener\">Overview, consequences, and strategies for overcoming matrix effects in LC-MS analysis: a critical review<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/36198988\/\" target=\"_blank\" rel=\"noreferrer noopener\">A review of the modern principles and applications of solid-phase extraction techniques in chromatographic analysis<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/scijournals.onlinelibrary.wiley.com\/doi\/full\/10.1002\/jctb.1794\" target=\"_blank\" rel=\"noreferrer noopener\">Liquid\u2013liquid extraction of biomolecules: an overview and update of the main techniques<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/bmc.2769\" target=\"_blank\" rel=\"noreferrer noopener\">Turbulent flow chromatography in bioanalysis: a review<\/a><\/li>\n<\/ol>\n\n\n\n<p>Visit us on <a href=\"https:\/\/www.linkedin.com\/showcase\/72003132\/admin\/dashboard\/\" target=\"_blank\" rel=\"noreferrer noopener\">LinkedIn:<\/a> #TurbulentflowChromatography #lcms #IonSuppression<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Too much interfering matrix? Discover how to streamline your daily LC-MS analysis with turbulent flow chromatography. Sample preparation is a critical step in liquid chromatography-mass spectrometry (LC-MS) analysis to achieve quantitative accuracy and precision. Directly injecting crude samples into your instrument without prior cleanup to remove interfering compounds can clog the flow path, damage your<\/p>\n","protected":false},"author":1760,"featured_media":18375,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[111,36,56],"tags":[1782,1748,37,1788,85],"division":[],"class_list":{"0":"post-18374","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-clinical-research-and-toxicology","8":"category-food-beverage","9":"category-pesticide-analysis","10":"tag-automated-sample-preparation","11":"tag-clinical-research-and-toxicology","12":"tag-food-beverage","13":"tag-forensic-toxicology","14":"tag-lc-ms","15":"entry"},"_selected_authors":[1969],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Automated LC-MS Sample Cleanup Using Turbulent Flow Chromatography - AnalyteGuru<\/title>\n<meta name=\"description\" content=\"Too much interfering matrix? 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