
{"id":5270,"date":"2020-04-09T00:00:00","date_gmt":"2020-04-09T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/analyteguru\/sports-doping-how-isotopes-help-to-fight-the-crime-part-2\/"},"modified":"2026-02-20T16:43:00","modified_gmt":"2026-02-20T16:43:00","slug":"sports-doping-how-isotopes-help-to-fight-the-crime-part-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/analyteguru\/sports-doping-how-isotopes-help-to-fight-the-crime-part-2\/","title":{"rendered":"Sports Doping How Isotopes Help to Fight the Crime &#8211; Part 2"},"content":{"rendered":"<div class=\"lia-message-body-content\">\n<p>Performance-enhancing drugs are substances that have the capability to improve performance in humans. In sports, those substances are sometimes referred to endogenic aids and one highly popular reference is anabolic steroids or their precursors with the colloquial term <a href=\"http:\/\/analyteguru.com\/athletes-on-the-anabolic-steriod-pump-a-review\/\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">steroids<\/a>.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignright wp-image-5272 size-medium\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/3737-og-300x200.jpg\" alt=\"\" width=\"300\" height=\"200\" srcset=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/3737-og-300x200.jpg 300w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/3737-og-768x512.jpg 768w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/3737-og-96x65.jpg 96w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/3737-og.jpg 999w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/>In our previous article, <a href=\"https:\/\/www.thermofisher.com\/blog\/analyteguru\/sports-doping-how-isotopes-help-to-fight-the-crime\/#:~:text=The%20origin%20of%20testosterone%20can,endogenous%20steroids%20in%20his%20body.\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">Sports Doping &#8212; How Isotopes Help to Fight the Crime<\/a>, we were talking about the history of performance-enhancing drugs, about challenges of doping analysis and how <a href=\"https:\/\/www.thermofisher.com\/de\/en\/home\/industrial\/mass-spectrometry\/isotope-ratio-mass-spectrometry-irms\/isotope-fingerprinting.html\" target=\"_blank\" rel=\"nofollow noopener\">isotope fingerprint<\/a>s help distinguish between naturally-produced steroids and synthetic analogues. The simple answer and the summery is\u0080\u00a6 We are what we eat and the stable isotopes signature in our bodies represents our diet and eating behavior.<\/p>\n<p>The <a href=\"https:\/\/www.thermofisher.com\/de\/en\/home\/industrial\/mass-spectrometry\/isotope-ratio-mass-spectrometry-irms\/isotope-fingerprinting.html\" target=\"_blank\" rel=\"nofollow noopener\">isotope fingerprint<\/a>, found in plants, for example, can vary according to plant type, but also according to region or rainfall. By eating those plants, carbon, hydrogen, and other elements are taken up by our bodies and converted into our own body tissue. Our own isotope fingerprint resembles therefore the fingerprint of our nutrition. Similarly, isotope fingerprints of our naturally produced steroids equals the isotope fingerprint of our body tissue. However, synthetic steroids do not! So, if the athlete is clean, stable isotope composition should remain identical among all steroids in his body. But if the athlete was taking synthetic testosterone, we will be able to see a difference in stable isotope value in the testosterone sample.<\/p>\n<p>Check out this whiteboard video about doping control with stable isotopes to get a great overview of how isotope analysis works.<\/p>\n<p><iframe loading=\"lazy\" title=\"Doping Control with the Isotope Hunter\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/C3NCCBmZGoU?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" allowfullscreen><\/iframe><\/p>\n<p>The analysis of testosterone misuses in sports is usually done with carbon isotopes (<sup>13<\/sup>C\/<sup>12<\/sup>C). Here, samples of testosterone are collected from urine and analyzed after sample preparation. <a href=\"https:\/\/www.thermofisher.com\/de\/de\/home\/industrial\/mass-spectrometry\/isotope-ratio-mass-spectrometry-irms.html\" target=\"_blank\" rel=\"nofollow noopener\">Isotope-ratio mass spectrometry (IRMS)<\/a> is the analytical technique for stable isotope analysis in organic compounds. In contrast to the standard mass spectrometry, as in example <a href=\"https:\/\/www.thermofisher.com\/de\/en\/home\/industrial\/mass-spectrometry\/gas-chromatography-mass-spectrometry-gc-ms.html\" target=\"_blank\" rel=\"nofollow noopener\">quadrupole MS or Orbitrap MS<\/a>, IRMS is capable of measuring the smallest differences in the isotope composition with high accuracy. And this is quite important since differences in isotope ratio occur in per mill range. Therefore, too small to detect with a standard MS.<\/p>\n<p>The IRMS is usually coupled to a gas chromatography (GC) or liquid chromatography (HPLC) for a prior separation of a compound mixture. Especially, this is useful for biological and environmental samples.<\/p>\n<p>Achieving the high accuracy isotope composition can only be measured after online compound conversion to a simple analyte gas &#8212; i.e. carbon to carbon dioxide. Carbon dioxide retains the isotopic information and is subsequently analyzed with IRMS with high precision.<\/p>\n<p>However, we must take into consideration the destructive nature of the methodology. To achieve the high accuracy, isotope composition can only be measured after online compound conversion to a simple analyte gas &#8212; i.e. carbon to carbon dioxide. One creative solution is <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/brochures\/AN-30405-GC-MS-IRMS-Sports-Drug-Testing-AN30405-EN.pdf\" target=\"_blank\" rel=\"nofollow noopener\">coupling GC-IRMS with high-resolution mass spectrometry for final confirmation in sports drug testin&#8230;<\/a>. This way structural information and stable isotopes can be measured simultaneously. Coupling mass spectrometers provides a tool to measure stable isotopes and performs structural elucidation simultaneously. Find out more about the final confirmation in sports drug testing <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/LSG\/brochures\/AN-30405-GC-MS-IRMS-Sports-Drug-Testing-AN30405-EN.pdf\" target=\"_blank\" rel=\"nofollow noopener\">here<\/a>.<\/p>\n<p>Table: <em>Elements for stable isotope analysis of doping. Source and interpretation.<\/em><\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-11257\" src=\"http:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/090420_BlogImage1.png\" alt=\"\" width=\"870\" height=\"264\" srcset=\"https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/090420_BlogImage1.png 870w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/090420_BlogImage1-300x91.png 300w, https:\/\/admin.acceleratingscience.com\/analyteguru\/wp-content\/uploads\/sites\/25\/2023\/06\/090420_BlogImage1-768x233.png 768w\" sizes=\"auto, (max-width: 870px) 100vw, 870px\" \/><\/p>\n<p>Except for carbon, hydrogen also can be applied for metabolite research. Identification of steroid metabolites in complex matrices can be time-consuming and challenging due to the requirement to both identify and quantify hundreds of different compounds with limited prior knowledge of the metabolites. Here compound-specific stable isotope analysis can be used to provide insights into the metabolism of many compounds in plants, animals and humans. Deuterium labeling of compounds has long been recognized as a valuable means to identify metabolites. GC-IRMS enables the compound-specific hydrogen isotope analysis at natural abundance and deuterium enrichment. Find out more about metabolite research <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/Application-Notes\/AN-30402-IRMS-Analyzers-AN30402-EN.pdf\" target=\"_blank\" rel=\"nofollow noopener\">here<\/a>.<\/p>\n<p>Are you performing analysis to uncover origins and authenticity of your samples?<\/p>\n<p>Access the e-learning collection of presentations and interviews of the Isotope Hunters, who are using isotope fingerprints to uncover the real history of their samples for:<\/p>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>food integrity and adulteration cases<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>environmental studies for the research of pollution origins and past climates<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>forensics applications for tracing animal and human movements<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<ul>\n<li style=\"list-style-type: none\">\n<ul>\n<li>&#8230; and much more<\/li>\n<\/ul>\n<\/li>\n<\/ul>\n<p>Become an Isotope Hunter! Attend the <a href=\"https:\/\/www.thermofisher.com\/de\/en\/home\/global\/forms\/industrial\/isotope-hunter-fingerprints-e-learning.html\" target=\"_blank\" rel=\"nofollow noopener\">Isotope Fingerprints e-learning sessions<\/a>.<\/p>\n<h3 id=\"toc-hId--1902365049\"><strong>Additional resources: <\/strong><\/h3>\n<p>Smart Note: <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/brochures\/sn-30519-irms-stable-isotopes-doping-control-investigations-sn30519-en.pdf\" target=\"_blank\" rel=\"nofollow noopener\">How do isotope fingerprints support doping control investigations?<\/a><\/p>\n<p>Application brief: <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/CMD\/Application-Notes\/ab-30520-gc-irms-isotopes-drugs-ab30520-en.pdf\" target=\"_blank\" rel=\"nofollow noopener\">GC-IRMS: Combat emerging threats in drug abuse with isotope fingerprints<\/a><\/p>\n<p>e-learning: <a href=\"https:\/\/www.thermofisher.com\/IsotopeFingerprintsElearning\" target=\"_blank\" rel=\"nofollow noopener\">Chapter about Isotope Fingerprints in Doping Control Investigations<\/a><\/p>\n<p>Webinar Series: <a href=\"https:\/\/www.thermofisher.com\/uk\/en\/home\/global\/forms\/industrial\/webinar-series--overcoming-challenges-in-sports-doping-analysis.html\" target=\"_blank\" rel=\"nofollow noopener\">Specific identification of exogenous steroids in urine by GC-IRMS<\/a><\/p>\n<p>Webinar Series: <a href=\"https:\/\/www.thermofisher.com\/de\/en\/home\/global\/forms\/industrial\/sessions-isotope-hunters-investigations.html\" target=\"_blank\" rel=\"nofollow noopener\">Isotope Hunter session &#8211; Uncover origin and authenticity of your samples<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Performance-enhancing drugs are substances that have the capability to improve performance in humans. In sports, those substances are sometimes<\/p>\n","protected":false},"author":1327,"featured_media":5272,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[57,1829],"tags":[471,59],"division":[],"class_list":{"0":"post-5270","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-gas-chromatography","8":"category-sports-doping","9":"tag-antidoping","10":"tag-gas-chromatography","11":"entry"},"_selected_authors":[],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Sports Doping How Isotopes Help to Fight the Crime - Part 2 - AnalyteGuru<\/title>\n<meta name=\"description\" content=\"Performance-enhancing drugs are substances that have the capability to improve performance in humans. 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