{"id":1224,"date":"2023-09-12T08:15:17","date_gmt":"2023-09-12T08:15:17","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/?p=1224"},"modified":"2023-11-27T18:41:06","modified_gmt":"2023-11-27T18:41:06","slug":"new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/","title":{"rendered":"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography"},"content":{"rendered":"

Biomedical research leverages volume electron microscopy techniques<\/strong><\/h2>\n

Volume electron microscopy allows biomedical researchers to study the 3D structure of cells, tissue and small organisms at nanometer resolution<\/a>.<\/p>\n

Because every organism has this type of multi-scale, 3D complexity, volume electron microscopy is relevant to understanding the secrets of human life and disease. It bridges the gap between light microscopy and cellular cryo-electron tomography and is extremely impactful for furthering 3D cell biology research.<\/p>\n

It was also named one of Nature<\/em>\u2019s Seven Technologies to Watch<\/a> in 2023.<\/p>\n

The expanding\u00a0community<\/a> requires more dedicated, multipurpose and customizable life science solutions to help maximize instrument run times, ensure predictable and reproducible results, and continue to advance this method sure to create scientific breakthroughs yet-to-be-imagined.<\/p>\n

Hydra Bio Plasma-FIB: A new dedicated solution for life sciences workflows <\/strong><\/h2>\n

We’re excited to introduce our new Thermo Scientific Hydra Bio Plasma-Focused Ion Beam<\/a> (Plasma-FIB) designed for cell biologists seeking simplified workflows while undertaking volume electron microscopy for cryo or resin-embedded samples.<\/p>\n

Building off the revered Thermo Scientific Helios Hydra DualBeam platform, the Hydra Bio Plasma-FIB joins the company\u2019s expanding cryo-FIB portfolio<\/a>, including a suite of tailored solutions for a variety of life science needs, and is designed to be a bridge from cryo-EM to room temperature analysis or vice versa.<\/p>\n

\n

Register to attend a webinar about the new Hydra Bio Plasma-FIB on October 18 >><\/em><\/a><\/p>\n

\n

From tissues to proteins, this versatile, multi-application instrument supports volume electron microscopy at cryogenic and room temperatures, as well as cellular cryo-electron tomography.<\/p>\n\n\n\n
\"Stefan<\/th>\n<\/th>\n\u201cMy lab is using cryo tomography to study the architecture of sarcomeres, the smallest subunits of striated muscle. Previously, we used isolated myofibrils to accomplish our goal. Now we are using the Hydra and cryo-lift out to be able to study muscle structures directly in the native muscle fibres.\u201d<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n

Dr. Stefan Raunser, Director, Structural Biochemistry, Max Planck Institute of Molecular Physiology. Image courtesy of North Rhine-Westphalian Academy of Sciences and Arts \/ Bettina Engel-Albustin 2022.<\/em><\/p>\n

The Hydra Bio Plasma-FIB addresses multiple workflows, including high-resolution serial PFIB imaging, including large area serial PFIB imaging using Thermo Fisher\u2019s unique Spin Mill Bio Method<\/a>, array tomography, correlative light and electron microscopy, and high-throughput lamella preparation for cryo-electron tomography.<\/p>\n

\"Thermo<\/p>\n

Spin Mill Bio Method: A Case Study<\/strong><\/h2>\n

The Spin Mill Bio Method used on the Hydra Bio Plasma-FIB allows researchers to uncover large sample areas and visualize regions of interest by providing large-area planar milling up to 1 mm in diameter, with a geometry like microtome-based serial block face imaging but at a slice thickness as small as 5 nm. Users can prepare clean, smooth surfaces used to localize regions of interest and subsequently image them in 2D or 3D.<\/p>\n

In this example<\/a>, by Watanabe et al, researchers used this method to study resin embedded mouse hippocampal synapses, high pressure frozen at different time points, and used tagging strategies to visualize different receptors and their relationship with synaptic vesicles.<\/p>\n

For each sample, they milled a 638 \u00b5m wide area view of the tissue and identified sparsely distributed synapses within the sample. The tagging of the receptors at these locations with gold enabled fast and easy end pointing and stacking of images.<\/p>\n

They then zoomed in on these regions of interest at high resolution, delivering images that they could use to measure the critical dimensions of the receptors in which they were interested.<\/p>\n

Being able to compare different samples, frozen at specific time points in a fast and time efficient manner, enabled a deeper understanding of synaptic function. The Hydra Bio Plasma-FIB has both the speed and the resolution needed to understand physiological processes at the nanometer level.<\/p>\n

Advancing cell biology research through technological innovation<\/strong><\/h2>\n

The further systematic buildup of a volume electron microscopy community will continue to fuel technological developments as well as provide access for increasingly large groups of bioscience researchers to benefit from these exciting and valuable techniques.<\/p>\n\n\n\n
\"Kirk<\/th>\n<\/th>\n\u201cWe are increasingly being challenged to understand how to improve crop resilience to environmental stress and disease. The remarkable capabilities of our new Hydra will allow us the unprecedented ability to reconstruct entire plant cells and tissue with exquisite detail. This technology will allow us to \u201cfreeze\u201d organisms in time and space and build intricate 3D models that will help us solve our critical food security challenges.\u201d<\/th>\n<\/tr>\n<\/tbody>\n<\/table>\n

Dr. Kirk J. Czymmek, Principal Investigator, Director Advanced Bioimaging Laboratory, Donald Danforth Plant Science Center<\/em><\/p>\n

At Thermo Fisher, we\u2019re focused on these technological developments so scientists can focus on what\u2019s most important\u2014their research and the positive impact it continues to have on our understanding of life and disease.<\/p>\n

With the new Hydra Bio Plasma-FIB, we\u2019re proud to offer cell biologists access to a life-science-dedicated cryo-FIB system that is easier to use and helps simplify room temperature and cryogenic workflows\u2014similar to what we\u2019ve developed for cryo-EM single particle analysis needs over the last decade.<\/p>\n

Learn more about the Thermo Scientific Hydra Bio Plasma-FIB >><\/em><\/a><\/p>\n

Register to attend \u2018Cryo-tomography to Volume EM: Explore with Hydra Bio Plasma-FIB\u2019 on Oct. 18 >><\/em><\/a><\/p>\n

\"Be<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Biomedical research leverages volume electron microscopy techniques Volume electron microscopy allows biomedical researchers to study the 3D structure of cells, tissue and small organisms at nanometer resolution. Because every organism has this type of multi-scale, 3D complexity, volume electron microscopy is relevant to understanding the secrets of human life and disease. It bridges the gap<\/p>\n","protected":false},"author":1391,"featured_media":1238,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[128,33,142],"tags":[129,228,229,226,227],"division":[],"class_list":{"0":"post-1224","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cryo-electron-tomography","8":"category-general","9":"category-in-the-news","10":"tag-cryo-electron-tomography","11":"tag-helios-hydra","12":"tag-pfib","13":"tag-volume-electron-microscopy","14":"tag-volume-em","15":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nVolume Electron Microscopy - PFIB - Cryo-ET - Life in Atomic Resolution<\/title>\n<meta name=\"description\" content=\"Thermo Scientific Hydra Bio Plasma-FIB for cell biology supports volume electron microscopy at cryo and room temperatures, as well as cryo-ET\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography\" \/>\n<meta property=\"og:description\" content=\"Thermo Scientific Hydra Bio Plasma-FIB for cell biology supports volume electron microscopy at cryo and room temperatures, as well as cryo-ET\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/\" \/>\n<meta property=\"og:site_name\" content=\"Life in Atomic Resolution\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2023-09-12T08:15:17+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2023-11-27T18:41:06+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Brianna Radicioni\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Brianna Radicioni\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/\"},\"author\":{\"name\":\"Brianna Radicioni\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#\\\/schema\\\/person\\\/2bd9f2cd8f714e3113d94c1612205906\"},\"headline\":\"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography\",\"datePublished\":\"2023-09-12T08:15:17+00:00\",\"dateModified\":\"2023-11-27T18:41:06+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/\"},\"wordCount\":847,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2023\\\/09\\\/Hydra-visual-1200x630-1.png\",\"keywords\":[\"cryo-electron tomography\",\"Helios Hydra\",\"PFIB\",\"volume electron microscopy\",\"volume-EM\"],\"articleSection\":[\"Cryo-Electron Tomography\",\"General\",\"In The News\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/\",\"name\":\"Volume Electron Microscopy - PFIB - Cryo-ET - Life in Atomic Resolution\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2023\\\/09\\\/Hydra-visual-1200x630-1.png\",\"datePublished\":\"2023-09-12T08:15:17+00:00\",\"dateModified\":\"2023-11-27T18:41:06+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#\\\/schema\\\/person\\\/2bd9f2cd8f714e3113d94c1612205906\"},\"description\":\"Thermo Scientific Hydra Bio Plasma-FIB for cell biology supports volume electron microscopy at cryo and room temperatures, as well as cryo-ET\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2023\\\/09\\\/Hydra-visual-1200x630-1.png\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2023\\\/09\\\/Hydra-visual-1200x630-1.png\",\"width\":1200,\"height\":630,\"caption\":\"Hydra Bio Plasma-FIB supports volume electron microscopy, cryo-electron tomography\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/\",\"name\":\"Life in Atomic Resolution\",\"description\":\"Electron and cryo-electron microscopy stories and solutions about the advancement of cellular and structural biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#\\\/schema\\\/person\\\/2bd9f2cd8f714e3113d94c1612205906\",\"name\":\"Brianna Radicioni\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/e8b33a5e67ca0918f85bde6cbe97f1d1c2e947932e5e7240aefa9feb0ba728b8?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/e8b33a5e67ca0918f85bde6cbe97f1d1c2e947932e5e7240aefa9feb0ba728b8?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/e8b33a5e67ca0918f85bde6cbe97f1d1c2e947932e5e7240aefa9feb0ba728b8?s=96&d=mm&r=g\",\"caption\":\"Brianna Radicioni\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/bradicioni\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Volume Electron Microscopy - PFIB - Cryo-ET - Life in Atomic Resolution","description":"Thermo Scientific Hydra Bio Plasma-FIB for cell biology supports volume electron microscopy at cryo and room temperatures, as well as cryo-ET","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/","og_locale":"en_US","og_type":"article","og_title":"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography","og_description":"Thermo Scientific Hydra Bio Plasma-FIB for cell biology supports volume electron microscopy at cryo and room temperatures, as well as cryo-ET","og_url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/","og_site_name":"Life in Atomic Resolution","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2023-09-12T08:15:17+00:00","article_modified_time":"2023-11-27T18:41:06+00:00","og_image":[{"width":1200,"height":630,"url":"http:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png","type":"image\/png"}],"author":"Brianna Radicioni","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Brianna Radicioni","Est. reading time":"5 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/"},"author":{"name":"Brianna Radicioni","@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#\/schema\/person\/2bd9f2cd8f714e3113d94c1612205906"},"headline":"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography","datePublished":"2023-09-12T08:15:17+00:00","dateModified":"2023-11-27T18:41:06+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/"},"wordCount":847,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png","keywords":["cryo-electron tomography","Helios Hydra","PFIB","volume electron microscopy","volume-EM"],"articleSection":["Cryo-Electron Tomography","General","In The News"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/","url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/","name":"Volume Electron Microscopy - PFIB - Cryo-ET - Life in Atomic Resolution","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png","datePublished":"2023-09-12T08:15:17+00:00","dateModified":"2023-11-27T18:41:06+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#\/schema\/person\/2bd9f2cd8f714e3113d94c1612205906"},"description":"Thermo Scientific Hydra Bio Plasma-FIB for cell biology supports volume electron microscopy at cryo and room temperatures, as well as cryo-ET","breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png","contentUrl":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png","width":1200,"height":630,"caption":"Hydra Bio Plasma-FIB supports volume electron microscopy, cryo-electron tomography"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/new-plasma-fib-volume-electron-microscopy-cryo-electron-tomography\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/"},{"@type":"ListItem","position":2,"name":"Thermo Fisher Scientific Announces New Plasma-FIB for Volume Electron Microscopy, Cryo-Electron Tomography"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#website","url":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/","name":"Life in Atomic Resolution","description":"Electron and cryo-electron microscopy stories and solutions about the advancement of cellular and structural biology","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#\/schema\/person\/2bd9f2cd8f714e3113d94c1612205906","name":"Brianna Radicioni","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/e8b33a5e67ca0918f85bde6cbe97f1d1c2e947932e5e7240aefa9feb0ba728b8?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/e8b33a5e67ca0918f85bde6cbe97f1d1c2e947932e5e7240aefa9feb0ba728b8?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/e8b33a5e67ca0918f85bde6cbe97f1d1c2e947932e5e7240aefa9feb0ba728b8?s=96&d=mm&r=g","caption":"Brianna Radicioni"},"url":"https:\/\/admin.acceleratingscience.com\/author\/bradicioni\/"}]}},"taxonomy_info":{"category":[{"value":128,"label":"Cryo-Electron Tomography"},{"value":33,"label":"General"},{"value":142,"label":"In The News"}],"post_tag":[{"value":129,"label":"cryo-electron tomography"},{"value":228,"label":"Helios Hydra"},{"value":229,"label":"PFIB"},{"value":226,"label":"volume electron microscopy"},{"value":227,"label":"volume-EM"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1-1024x538.png",760,399,true],"author_info":{"display_name":"Brianna Radicioni","author_link":"https:\/\/admin.acceleratingscience.com\/author\/bradicioni\/"},"comment_info":0,"category_info":[{"term_id":128,"name":"Cryo-Electron Tomography","slug":"cryo-electron-tomography","term_group":0,"term_taxonomy_id":128,"taxonomy":"category","description":"","parent":24,"count":16,"filter":"raw","meta":[],"cat_ID":128,"category_count":16,"category_description":"","cat_name":"Cryo-Electron Tomography","category_nicename":"cryo-electron-tomography","category_parent":24},{"term_id":33,"name":"General","slug":"general","term_group":0,"term_taxonomy_id":33,"taxonomy":"category","description":"","parent":0,"count":59,"filter":"raw","meta":[],"cat_ID":33,"category_count":59,"category_description":"","cat_name":"General","category_nicename":"general","category_parent":0},{"term_id":142,"name":"In The News","slug":"in-the-news","term_group":0,"term_taxonomy_id":142,"taxonomy":"category","description":"","parent":0,"count":4,"filter":"raw","meta":[],"cat_ID":142,"category_count":4,"category_description":"","cat_name":"In The News","category_nicename":"in-the-news","category_parent":0}],"tag_info":[{"term_id":129,"name":"cryo-electron tomography","slug":"cryo-electron-tomography","term_group":0,"term_taxonomy_id":129,"taxonomy":"post_tag","description":"","parent":0,"count":15,"filter":"raw","meta":[]},{"term_id":228,"name":"Helios Hydra","slug":"helios-hydra","term_group":0,"term_taxonomy_id":228,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","meta":[]},{"term_id":229,"name":"PFIB","slug":"pfib","term_group":0,"term_taxonomy_id":229,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","meta":[]},{"term_id":226,"name":"volume electron microscopy","slug":"volume-electron-microscopy","term_group":0,"term_taxonomy_id":226,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","meta":[]},{"term_id":227,"name":"volume-EM","slug":"volume-em","term_group":0,"term_taxonomy_id":227,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":2117,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/advances-in-cryo-electron-tomography-expand-the-capabilities-of-quantitative-cell-biology\/","url_meta":{"origin":1224,"position":0},"title":"Advances in cryo-electron tomography expand the capabilities of quantitative cell biology","author":"Vinay S","date":"September 29, 2026","format":false,"excerpt":"Key takeaways Cryo-electron tomography has largely been seen as a qualitative method because cryo-ET data segmentation has been a highly intensive, manual process. Recent advances in deep-learning-based segmentation have expanded the capabilities of cryo-electron tomography, supporting quantitative analysis of cellular structures. A recent webinar by Professor Danielle Grotjahn highlights how\u2026","rel":"","context":"In "Cryo EM Techniques"","block_context":{"text":"Cryo EM Techniques","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/cryo-em-techniques\/"},"img":{"alt_text":"Advances in cryo-electron tomography expand the capabilities of quantitative cell biology.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":2143,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/microscopy-image-analysis-with-amira-software-ai-in-cell-biology\/","url_meta":{"origin":1224,"position":1},"title":"Four ways AI is making microscopy image analysis easier for cell biologists","author":"Alejandra Sanchez-Erostegui","date":"October 14, 2026","format":false,"excerpt":"Key takeaways Modern microscopes are capable of generating large volumes of high-resolution data, which is highly valuable for cell biology research, but also makes it increasingly more laborious and difficult to extract meaningful scientific information from the whole dataset.\u00a0 Artificial intelligence is well positioned to support this work by streamlining\u2026","rel":"","context":"In "Amira Software"","block_context":{"text":"Amira Software","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/amira-software\/"},"img":{"alt_text":"Four ways AI is making microscopy image analysis easier for cell biologists.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/fault-localization-root-cause-ai-packaging-header-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/fault-localization-root-cause-ai-packaging-header-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/fault-localization-root-cause-ai-packaging-header-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/fault-localization-root-cause-ai-packaging-header-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/fault-localization-root-cause-ai-packaging-header-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/fault-localization-root-cause-ai-packaging-header-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":1854,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/advances-in-100-kv-high-resolution-cryo-em-single-particle-analysis\/","url_meta":{"origin":1224,"position":2},"title":"Advances in high-resolution cryo-EM at 100 kV","author":"Swarnim Rawat","date":"February 5, 2026","format":false,"excerpt":"100 kV cryo-TEM enables high-resolution single particle analysis Within the past decade, cryo-electron microscopy (cryo-EM) techniques have revolutionized structural biology by enabling the visualization of challenging molecular and sub-cellular structures in their near-native states at high resolution. These valuable observations have, until recently, been relegated to advanced 300 kV instruments\u2026","rel":"","context":"In "Cryo-Electron Microscopy (Cryo-EM)"","block_context":{"text":"Cryo-Electron Microscopy (Cryo-EM)","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/"},"img":{"alt_text":"Advances in high-resolution cryo-EM at 100 kV","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":2172,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-subtomogram-averaging\/","url_meta":{"origin":1224,"position":3},"title":"What is Subtomogram Averaging?","author":"Swarnim Rawat","date":"October 19, 2026","format":false,"excerpt":"Turning crowded cryo-ET data into structural insight with subtomogram averaging Cryo-electron tomography (cryo-ET) is capable of capturing detailed, three-dimensional views of vitrified biological samples, supporting a broad range of life sciences research. Subtomogram averaging helps refine that crowded data for clearer structural insight by finding repeated particles, extracting them as\u2026","rel":"","context":"In "Cancer Research"","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/amira-software\/cancer-research\/"},"img":{"alt_text":"Social Cryo EM","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/social.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/social.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/social.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/10\/social.png?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":1935,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/fragment-based-drug-discovery-of-drug-targets-membrane-proteins-with-cryo-em\/","url_meta":{"origin":1224,"position":4},"title":"Fragment based drug discovery meets challenging drug targets with high-throughput cryo-EM\u00a0","author":"Swarnim Rawat","date":"December 12, 2025","format":false,"excerpt":"Benefits of FBDD in the search for novel therapeutics\u00a0 Fragment-based drug discovery (FBDD) is a method that screens a large quantity of protein fragments for their potential as small molecule therapeutics. This approach was previously hampered by the slow throughput of crystallization for the characterization of potential leads. The increasing\u2026","rel":"","context":"In "Cryo EM Drug Discovery"","block_context":{"text":"Cryo EM Drug Discovery","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/cryo-em-drug-discovery\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":1953,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/3d-tissue-histology-light-sheet-microscopy-for-nondestructive-microglia-structure-analysis\/","url_meta":{"origin":1224,"position":5},"title":"3D Tissue Histology with Light-Sheet Microscopy Enables Nondestructive Analysis of Microglia","author":"Vinay S","date":"January 21, 2026","format":false,"excerpt":"3D tissue analysis offers critical benefits for neuroscience research The imaging and analysis of tissues is vital across a number of biological fields, particularly neuroscience, where the accurate characterization of neural tissue plays an important role in our understanding of the nervous system. Historically, these samples were imaged as thin\u2026","rel":"","context":"In "Amira Software"","block_context":{"text":"Amira Software","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/amira-software\/"},"img":{"alt_text":"3D Tissue Histology with Light-Sheet Microscopy Enables Nondestructive Analysis of Microglia","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2023\/09\/Hydra-visual-1200x630-1.png","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/posts\/1224","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/users\/1391"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/comments?post=1224"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/posts\/1224\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/media\/1238"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/media?parent=1224"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/categories?post=1224"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/tags?post=1224"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/division?post=1224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}