{"id":1545,"date":"2024-10-30T02:00:28","date_gmt":"2024-10-30T02:00:28","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/?p=1545"},"modified":"2026-02-19T21:41:58","modified_gmt":"2026-02-19T21:41:58","slug":"what-is-cryo-em-what-is-cryo-electron-microscopy","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/","title":{"rendered":"What is cryo-EM?"},"content":{"rendered":"

Cryo-electron microscopy<\/a> (cryo-EM) describes a collection of techniques used to analyze cryogenically frozen samples in specially designed transmission electron microscopes (cryo-TEMs). Recent advancements, including improved sample preparation tools, detectors, and analytical computer algorithms, have even enabled the determination of 3D cryo-EM structures at atomic resolution.<\/p>\n

\"Single

General workflow used in single particle analysis, a common cryo-EM technique.<\/p><\/div>\n

How does cryo-EM work?<\/strong><\/h2>\n

When samples are flash frozen (vitrified), the aqueous solution does not form crystalline ice, instead becoming a vitreous solid. This reduces the damage that the freezing process causes to the sensitive biological sample, thereby taking a \u201csnapshot\u201d of the specimen in solution.<\/p>\n

Broadly speaking, cryo-EM techniques<\/a> then image these samples at a variety of angles at high resolution and then digitally recombine the 2D snapshots into a 3D reconstruction. Cryo-EM can determine structures of challenging proteins and other macromolecules while providing wholly unique insights into how these proteins function. Although once considered a specialty tool used only by a small group of experts, cryo-electron microscopes are now increasingly easier to use, more affordable, and accessible to the wider scientific community.<\/p>\n

Benefits of cryo-EM<\/strong><\/h2>\n

Cryo-electron microscopy fills a complimentary niche next to other popular structural analysis techniques such as X-ray diffraction and nuclear magnetic resonance. Compared to other techniques, cryo-EM doesn\u2019t require samples to be crystallized, which has been a significant barrier in the analysis of large complexes and membrane protein<\/a>. Typically, all that\u2019s required for cryo-EM is that the samples can be suspended in an aqueous environment.<\/p>\n

Additionally, other techniques that require extensive sample preparation always carry the risk of subtly disrupting the biologically accurate structures as they exist in vivo. Through vitrification, this risk is significantly lowered, thereby providing physiologically relevant observations.<\/p>\n

\"Comparison

Comparison of common methods for imaging and structural analysis used in the life sciences, including cryo-electron microscopy techniques.<\/p><\/div>\n

Cryo-EM techniques<\/strong><\/h2>\n

While cryo-EM is sometimes used synonymously with the most popular technique, single particle analysis, there are a suite of analytical methods that can be performed in a cryo-TEM. We will provide a brief introduction to them here; far more information is available on our website.<\/p>\n

\"Histamine

Histamine in the binding pocket of human GABA-A (resolved at 1.7 \u00c5 with the Krios Cryo-TEM).
Data courtesy A. Sente and R. Aricescu, MRC-LMB Cambridge.<\/p><\/div>\n

Single particle analysis<\/strong><\/h2>\n

In single particle analysis, purified proteins or protein complexes are first vitrified to preserve their native structures. TEM is then used to collect numerous 2D snapshots of the samples. As the proteins are oriented randomly within the ice, these images show the sample at various angles, and can be recombined into a high-resolution 3D reconstruction of the sample.<\/p>\n

\"Cryo-electron

Cryo-electron tomography visualization of a Golgi apparatus from the green algae Chlamydomonas reinhardtii<\/em>.<\/p><\/div>\n

Cryo-tomography<\/strong><\/h2>\n

Cryo-electron tomography (cryo-ET), most commonly applied to whole cells, provides 3D visualization of organelles and protein complexes at nanometer resolution in their physiological environments. This is done by opening windows into the cell with focused ion beam (FIB) milling of a vitrified cell. A series of 2D images are taken of this thinned cellular sample, which are then reconstructed into a 3D dataset. Such high-resolution 3D images of the cell interior can provide new insights into cellular function and sheds light on the arrangement and structure of native protein complexes.<\/p>\n

\"Protein

Protein structure determination with microcrystal electron diffraction.<\/p><\/div>\n

Microcrystal electron diffraction<\/strong><\/h2>\n

Microcrystal electron diffraction, or MicroED, is a diffraction technique similar to X-ray crystallography (XRD) that utilizes electrons rather than X-rays. Due to electron\u2019s smaller wavelength and stronger interaction with the sample, atomic details can be extracted from individual nanocrystals (<200 nm in size), even in a heterogeneous mixture. This allows for the analysis of crystals that are too small for XRD and other crystallography methods. Data collection is completed in only a few minutes, and 3D structures can be determined at atomic resolution.<\/p>\n

Conclusions<\/strong><\/h2>\n

We hope this brief introduction has given you a glimpse into the power and possibilities of cryo-electron microscopy. With this revolutionary technology, structural biologists can analyze proteins in all their complex conformations, structures, and modified forms. They can even look at multiple protein conformations or heterogeneous complexes in a single sample.<\/p>\n

If you\u2019d like to know more about cryo-electron microscopy and the ways in which it is revolutionizing structural biology, pharmaceutical research, and more, please see some of our other educational posts on our Life in Atomic Resolution blog:<\/p>\n

– Seeing with Electrons: The Anatomy of an Electron Microscope<\/a>
– Open Access: Cellular Cryo-Electron Tomography Education<\/a>
– Cryo-EM Sample Preparation: A Q&A with University of Utah\u2019s Core Lab Director<\/a><\/p>\n

\u00a0<\/p>\n

For even more educational resources, visit our Life Sciences EM Learning Center >><\/a><\/strong><\/p>\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Cryo-electron microscopy (cryo-EM) describes a collection of techniques used to analyze cryogenically frozen samples in specially designed transmission electron microscopes (cryo-TEMs). Recent advancements, including improved sample preparation tools, detectors, and analytical computer algorithms, have even enabled the determination of 3D cryo-EM structures at atomic resolution. How does cryo-EM work? When samples are flash frozen (vitrified),<\/p>\n","protected":false},"author":905,"featured_media":1551,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[330,24,128,14,116,329],"tags":[331,16,134,332,76],"division":[],"class_list":{"0":"post-1545","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cryo-em-techniques","8":"category-cryo-electron-microscopy-cryo-em","9":"category-cryo-electron-tomography","10":"category-electron-microscopy-101","11":"category-microed","12":"category-single-particle-analysis","13":"tag-cryo-em-techniques","14":"tag-cryo-em","15":"tag-cryo-tomography","16":"tag-microcrystal-electron-diffraction","17":"tag-single-particle-analysis","18":"entry"},"_selected_authors":[1292],"_selected_reviewers":[],"acf":[],"yoast_head":"\nWhat is Cryo EM? - What is Cryo Electron Microscopy? - Cryo EM - Life in Atomic Resolution<\/title>\n<meta name=\"description\" content=\"What is cryo EM? A collection of techniques for the analysis of cryogenically frozen samples in specialized transmission electron microscopes\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"What is cryo-EM?\" \/>\n<meta property=\"og:description\" content=\"What is cryo EM? A collection of techniques for the analysis of cryogenically frozen samples in specialized transmission electron microscopes\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/\" \/>\n<meta property=\"og:site_name\" content=\"Life in Atomic Resolution\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2024-10-30T02:00:28+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2026-02-19T21:41:58+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1920\" \/>\n\t<meta property=\"og:image:height\" content=\"600\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Alex Ilitchev\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Alex Ilitchev\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"5 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/\"},\"author\":{\"name\":\"Alex Ilitchev\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#\\\/schema\\\/person\\\/7b14b1ad2e6aaf9f989f5fb345363e39\"},\"headline\":\"What is cryo-EM?\",\"datePublished\":\"2024-10-30T02:00:28+00:00\",\"dateModified\":\"2026-02-19T21:41:58+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/\"},\"wordCount\":808,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2024\\\/10\\\/cryoEM-blog-virus-pore4-1920x600_Header.jpg\",\"keywords\":[\"Cryo EM Techniques\",\"cryo-EM\",\"cryo-tomography\",\"Microcrystal Electron Diffraction\",\"single particle analysis\"],\"articleSection\":[\"Cryo EM Techniques\",\"Cryo-Electron Microscopy (Cryo-EM)\",\"Cryo-Electron Tomography\",\"Electron Microscopy 101\",\"MicroED\",\"Single Particle Analysis\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/\",\"name\":\"What is Cryo EM? - What is Cryo Electron Microscopy? - Cryo EM - Life in Atomic Resolution\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2024\\\/10\\\/cryoEM-blog-virus-pore4-1920x600_Header.jpg\",\"datePublished\":\"2024-10-30T02:00:28+00:00\",\"dateModified\":\"2026-02-19T21:41:58+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#\\\/schema\\\/person\\\/7b14b1ad2e6aaf9f989f5fb345363e39\"},\"description\":\"What is cryo EM? A collection of techniques for the analysis of cryogenically frozen samples in specialized transmission electron microscopes\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2024\\\/10\\\/cryoEM-blog-virus-pore4-1920x600_Header.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/wp-content\\\/uploads\\\/sites\\\/22\\\/2024\\\/10\\\/cryoEM-blog-virus-pore4-1920x600_Header.jpg\",\"width\":1920,\"height\":600,\"caption\":\"cryoEM-blog-virus pore\"},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/atomic-resolution\\\/what-is-cryo-em-what-is-cryo-electron-microscopy\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"What is cryo-EM?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/\",\"name\":\"Life in Atomic Resolution\",\"description\":\"Electron and cryo-electron microscopy stories and solutions about the advancement of cellular and structural biology\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/atomic-resolution\\\/#\\\/schema\\\/person\\\/7b14b1ad2e6aaf9f989f5fb345363e39\",\"name\":\"Alex Ilitchev\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/54cef1351cde5037d69bb7709c905d8c8159926c7a8c061899c34f746bb02e6f?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/54cef1351cde5037d69bb7709c905d8c8159926c7a8c061899c34f746bb02e6f?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/54cef1351cde5037d69bb7709c905d8c8159926c7a8c061899c34f746bb02e6f?s=96&d=mm&r=g\",\"caption\":\"Alex Ilitchev\"},\"description\":\"Alex Ilitchev, PhD, is a science writer at Thermo Fisher Scientific. They have a background in biophysical chemistry, with a focus on protein misfolding, aggregation, as well as mass spectrometry. Alex supports writing efforts across a variety of analytical instruments and software from Thermo Fisher Scientific, including electron microscopy in the life and materials sciences.\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/alexilitchev\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"What is Cryo EM? - What is Cryo Electron Microscopy? - Cryo EM - Life in Atomic Resolution","description":"What is cryo EM? A collection of techniques for the analysis of cryogenically frozen samples in specialized transmission electron microscopes","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/","og_locale":"en_US","og_type":"article","og_title":"What is cryo-EM?","og_description":"What is cryo EM? A collection of techniques for the analysis of cryogenically frozen samples in specialized transmission electron microscopes","og_url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/","og_site_name":"Life in Atomic Resolution","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2024-10-30T02:00:28+00:00","article_modified_time":"2026-02-19T21:41:58+00:00","og_image":[{"width":1920,"height":600,"url":"http:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg","type":"image\/jpeg"}],"author":"Alex Ilitchev","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Alex Ilitchev","Est. reading time":"5 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/"},"author":{"name":"Alex Ilitchev","@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#\/schema\/person\/7b14b1ad2e6aaf9f989f5fb345363e39"},"headline":"What is cryo-EM?","datePublished":"2024-10-30T02:00:28+00:00","dateModified":"2026-02-19T21:41:58+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/"},"wordCount":808,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg","keywords":["Cryo EM Techniques","cryo-EM","cryo-tomography","Microcrystal Electron Diffraction","single particle analysis"],"articleSection":["Cryo EM Techniques","Cryo-Electron Microscopy (Cryo-EM)","Cryo-Electron Tomography","Electron Microscopy 101","MicroED","Single Particle Analysis"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/","url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/","name":"What is Cryo EM? - What is Cryo Electron Microscopy? - Cryo EM - Life in Atomic Resolution","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg","datePublished":"2024-10-30T02:00:28+00:00","dateModified":"2026-02-19T21:41:58+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#\/schema\/person\/7b14b1ad2e6aaf9f989f5fb345363e39"},"description":"What is cryo EM? A collection of techniques for the analysis of cryogenically frozen samples in specialized transmission electron microscopes","breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg","width":1920,"height":600,"caption":"cryoEM-blog-virus pore"},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/what-is-cryo-em-what-is-cryo-electron-microscopy\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/"},{"@type":"ListItem","position":2,"name":"What is cryo-EM?"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#website","url":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/","name":"Life in Atomic Resolution","description":"Electron and cryo-electron microscopy stories and solutions about the advancement of cellular and structural biology","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/#\/schema\/person\/7b14b1ad2e6aaf9f989f5fb345363e39","name":"Alex Ilitchev","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/54cef1351cde5037d69bb7709c905d8c8159926c7a8c061899c34f746bb02e6f?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/54cef1351cde5037d69bb7709c905d8c8159926c7a8c061899c34f746bb02e6f?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/54cef1351cde5037d69bb7709c905d8c8159926c7a8c061899c34f746bb02e6f?s=96&d=mm&r=g","caption":"Alex Ilitchev"},"description":"Alex Ilitchev, PhD, is a science writer at Thermo Fisher Scientific. They have a background in biophysical chemistry, with a focus on protein misfolding, aggregation, as well as mass spectrometry. Alex supports writing efforts across a variety of analytical instruments and software from Thermo Fisher Scientific, including electron microscopy in the life and materials sciences.","url":"https:\/\/admin.acceleratingscience.com\/author\/alexilitchev\/"}]}},"taxonomy_info":{"category":[{"value":330,"label":"Cryo EM Techniques"},{"value":24,"label":"Cryo-Electron Microscopy (Cryo-EM)"},{"value":128,"label":"Cryo-Electron Tomography"},{"value":14,"label":"Electron Microscopy 101"},{"value":116,"label":"MicroED"},{"value":329,"label":"Single Particle Analysis"}],"post_tag":[{"value":331,"label":"Cryo EM Techniques"},{"value":16,"label":"cryo-EM"},{"value":134,"label":"cryo-tomography"},{"value":332,"label":"Microcrystal Electron Diffraction"},{"value":76,"label":"single particle analysis"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header-1024x320.jpg",760,238,true],"author_info":{"display_name":"Alex Ilitchev","author_link":"https:\/\/admin.acceleratingscience.com\/author\/alexilitchev\/"},"comment_info":2,"category_info":[{"term_id":330,"name":"Cryo EM Techniques","slug":"cryo-em-techniques","term_group":0,"term_taxonomy_id":330,"taxonomy":"category","description":"","parent":24,"count":4,"filter":"raw","meta":[],"cat_ID":330,"category_count":4,"category_description":"","cat_name":"Cryo EM Techniques","category_nicename":"cryo-em-techniques","category_parent":24},{"term_id":24,"name":"Cryo-Electron Microscopy (Cryo-EM)","slug":"cryo-electron-microscopy-cryo-em","term_group":0,"term_taxonomy_id":24,"taxonomy":"category","description":"","parent":0,"count":78,"filter":"raw","meta":[],"cat_ID":24,"category_count":78,"category_description":"","cat_name":"Cryo-Electron Microscopy (Cryo-EM)","category_nicename":"cryo-electron-microscopy-cryo-em","category_parent":0},{"term_id":128,"name":"Cryo-Electron Tomography","slug":"cryo-electron-tomography","term_group":0,"term_taxonomy_id":128,"taxonomy":"category","description":"","parent":24,"count":15,"filter":"raw","meta":[],"cat_ID":128,"category_count":15,"category_description":"","cat_name":"Cryo-Electron Tomography","category_nicename":"cryo-electron-tomography","category_parent":24},{"term_id":14,"name":"Electron Microscopy 101","slug":"electron-microscopy-101","term_group":0,"term_taxonomy_id":14,"taxonomy":"category","description":"","parent":0,"count":5,"filter":"raw","meta":[],"cat_ID":14,"category_count":5,"category_description":"","cat_name":"Electron Microscopy 101","category_nicename":"electron-microscopy-101","category_parent":0},{"term_id":116,"name":"MicroED","slug":"microed","term_group":0,"term_taxonomy_id":116,"taxonomy":"category","description":"","parent":24,"count":4,"filter":"raw","meta":[],"cat_ID":116,"category_count":4,"category_description":"","cat_name":"MicroED","category_nicename":"microed","category_parent":24},{"term_id":329,"name":"Single Particle Analysis","slug":"single-particle-analysis","term_group":0,"term_taxonomy_id":329,"taxonomy":"category","description":"","parent":24,"count":2,"filter":"raw","meta":[],"cat_ID":329,"category_count":2,"category_description":"","cat_name":"Single Particle Analysis","category_nicename":"single-particle-analysis","category_parent":24}],"tag_info":[{"term_id":331,"name":"Cryo EM Techniques","slug":"cryo-em-techniques","term_group":0,"term_taxonomy_id":331,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","meta":[]},{"term_id":16,"name":"cryo-EM","slug":"cryo-em","term_group":0,"term_taxonomy_id":16,"taxonomy":"post_tag","description":"","parent":0,"count":63,"filter":"raw","meta":[]},{"term_id":134,"name":"cryo-tomography","slug":"cryo-tomography","term_group":0,"term_taxonomy_id":134,"taxonomy":"post_tag","description":"","parent":0,"count":2,"filter":"raw","meta":[]},{"term_id":332,"name":"Microcrystal Electron Diffraction","slug":"microcrystal-electron-diffraction","term_group":0,"term_taxonomy_id":332,"taxonomy":"post_tag","description":"","parent":0,"count":1,"filter":"raw","meta":[]},{"term_id":76,"name":"single particle analysis","slug":"single-particle-analysis","term_group":0,"term_taxonomy_id":76,"taxonomy":"post_tag","description":"","parent":0,"count":5,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":1854,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/advances-in-100-kv-high-resolution-cryo-em-single-particle-analysis\/","url_meta":{"origin":1545,"position":0},"title":"Advances in high-resolution cryo-EM at 100 kV","author":"Swarnim Rawat","date":"February 5, 2026","format":false,"excerpt":"100 kV cryo-TEM enables high-resolution single particle analysis Within the past decade, cryo-electron microscopy (cryo-EM) techniques have revolutionized structural biology by enabling the visualization of challenging molecular and sub-cellular structures in their near-native states at high resolution. These valuable observations have, until recently, been relegated to advanced 300 kV instruments\u2026","rel":"","context":"In "Cryo-Electron Microscopy (Cryo-EM)"","block_context":{"text":"Cryo-Electron Microscopy (Cryo-EM)","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/"},"img":{"alt_text":"Advances in high-resolution cryo-EM at 100 kV","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/08\/Blog-Tundra-Preprint-1858725-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":2117,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/advances-in-cryo-electron-tomography-expand-the-capabilities-of-quantitative-cell-biology\/","url_meta":{"origin":1545,"position":1},"title":"Advances in cryo-electron tomography expand the capabilities of quantitative cell biology","author":"Vinay S","date":"September 29, 2026","format":false,"excerpt":"Key takeaways Cryo-electron tomography has largely been seen as a qualitative method because cryo-ET data segmentation has been a highly intensive, manual process. Recent advances in deep-learning-based segmentation have expanded the capabilities of cryo-electron tomography, supporting quantitative analysis of cellular structures. A recent webinar by Professor Danielle Grotjahn highlights how\u2026","rel":"","context":"In "Cryo EM Techniques"","block_context":{"text":"Cryo EM Techniques","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/cryo-em-techniques\/"},"img":{"alt_text":"Advances in cryo-electron tomography expand the capabilities of quantitative cell biology.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/09\/blog-cryo-em-series-header-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":1935,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/fragment-based-drug-discovery-of-drug-targets-membrane-proteins-with-cryo-em\/","url_meta":{"origin":1545,"position":2},"title":"Fragment based drug discovery meets challenging drug targets with high-throughput cryo-EM\u00a0","author":"Swarnim Rawat","date":"December 12, 2025","format":false,"excerpt":"Benefits of FBDD in the search for novel therapeutics\u00a0 Fragment-based drug discovery (FBDD) is a method that screens a large quantity of protein fragments for their potential as small molecule therapeutics. This approach was previously hampered by the slow throughput of crystallization for the characterization of potential leads. The increasing\u2026","rel":"","context":"In "Cryo EM Drug Discovery"","block_context":{"text":"Cryo EM Drug Discovery","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/cryo-em-drug-discovery\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2025\/12\/blog-8-banner-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":2016,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/how-artificial-intelligence-ai-improves-image-analysis-workflows-in-life-sciences\/","url_meta":{"origin":1545,"position":3},"title":"How artificial intelligence (AI) improves image analysis workflows in life sciences","author":"Vinay S","date":"June 2, 2026","format":false,"excerpt":"Image acquisition is not the end of the story\u2014it is the starting point. Once images are captured, researchers typically move into preprocessing to improve quality, then they perform visualization, segmentation, and quantitative analysis to extract meaningful measurements. The goal is simple: turn images into understanding. In practice, however, image analysis\u2026","rel":"","context":"In "Amira Software"","block_context":{"text":"Amira Software","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/amira-software\/"},"img":{"alt_text":"How artificial intelligence (AI) improves image analysis workflows in life sciences","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/06\/amira-ai-tools-blog-header-1920x450-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/06\/amira-ai-tools-blog-header-1920x450-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/06\/amira-ai-tools-blog-header-1920x450-1.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/06\/amira-ai-tools-blog-header-1920x450-1.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/06\/amira-ai-tools-blog-header-1920x450-1.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/06\/amira-ai-tools-blog-header-1920x450-1.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":1953,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/3d-tissue-histology-light-sheet-microscopy-for-nondestructive-microglia-structure-analysis\/","url_meta":{"origin":1545,"position":4},"title":"3D Tissue Histology with Light-Sheet Microscopy Enables Nondestructive Analysis of Microglia","author":"Vinay S","date":"January 21, 2026","format":false,"excerpt":"3D tissue analysis offers critical benefits for neuroscience research The imaging and analysis of tissues is vital across a number of biological fields, particularly neuroscience, where the accurate characterization of neural tissue plays an important role in our understanding of the nervous system. Historically, these samples were imaged as thin\u2026","rel":"","context":"In "Amira Software"","block_context":{"text":"Amira Software","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/amira-software\/"},"img":{"alt_text":"3D Tissue Histology with Light-Sheet Microscopy Enables Nondestructive Analysis of Microglia","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/01\/neuroscience-blog-header-1920x450-1.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":2071,"url":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/facilitating-soft-tissue-analysis-unraveling-complex-microct-imaging-data-with-amira-software\/","url_meta":{"origin":1545,"position":5},"title":"Facilitating Soft Tissue Analysis \u2013 Unraveling Complex MicroCT Imaging Data with Amira Software","author":"Vinay S","date":"August 27, 2026","format":false,"excerpt":"Key takeaways Micro-computed X-ray tomography (microCT) can be used for high-resolution, 3D visualization of a range of biological samples, including soft tissues, but transforming this data into scientific insights requires the processing of increasingly large, complex datasets. Thermo Scientific Amira Software is a powerful data processing tool that can simplify\u2026","rel":"","context":"In "Amira Software"","block_context":{"text":"Amira Software","link":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/cryo-electron-microscopy-cryo-em\/amira-software\/"},"img":{"alt_text":"Facilitating Soft Tissue Analysis \u2013 Unraveling Complex MicroCT Imaging Data with Amira Software","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/08\/blog-soft-tissue-micro-ct-research-header-1920x450-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/08\/blog-soft-tissue-micro-ct-research-header-1920x450-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/08\/blog-soft-tissue-micro-ct-research-header-1920x450-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/08\/blog-soft-tissue-micro-ct-research-header-1920x450-1.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/08\/blog-soft-tissue-micro-ct-research-header-1920x450-1.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2026\/08\/blog-soft-tissue-micro-ct-research-header-1920x450-1.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/atomic-resolution\/wp-content\/uploads\/sites\/22\/2024\/10\/cryoEM-blog-virus-pore4-1920x600_Header.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/posts\/1545","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/users\/905"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/comments?post=1545"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/posts\/1545\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/media\/1551"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/media?parent=1545"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/categories?post=1545"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/tags?post=1545"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/atomic-resolution\/wp-json\/wp\/v2\/division?post=1545"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}