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Accelerating ScienceBehind the Bench / Product News / The Next Generation of PCR Enzymes: Purity and accuracy with DNA-free PCR enzymes

The Next Generation of PCR Enzymes: Purity and accuracy with DNA-free PCR enzymes

By 09.06.2017

Molecular diagnostics, a driving force in health care, relies heavily on tests utilizing Polymerase Chain Reaction (PCR) technology. PCR enables sensitive detection, specific identification, as well as accurate quantification of disease causing agents. To avoid false positives and uncertainty in the assay results, it is crucial that the reagents used in any PCR test are free of foreign DNA; such as DNA originating from the manufacturing environment or human operator, the host DNA of recombinant protein-expressing cells, or carry over DNA from shared manufacturing equipment.

DNA-Free White Paper

Thermo Fisher Scientific has developed a new manufacturing paradigm that delivers a much purer PCR reagent enabling better diagnostic assays. This new process to manufacture DNA-free enzymes incorporate  a closed and single-use system based technology to remove the risk of DNA contamination inherent to the conventional manufacturing process1. To ensure conformance to strict purity requirements expected by molecular diagnostics developers, we subject our DNA-free PCR reagents to stringent quality tests to ensure reagents are free of contaminating bacterial, human and plasmid-born DNA.  This combination of manufacturing systems and quality testing delivers PCR reagents that are 100X cleaner than other DNA-free reagents on the market.

For more information about single-use system manufacturing of DNA-free PCR enzymes, download the white paper.

For Research Use Only.  Not for use in diagnostic procedures.

1Spangler R, Goddard NL, Thaler DS (2009) Optimizing Taq polymerase concentration for improved signal-to-noise in the broad-range detection of low-abundance bacteria. PLoS One 4:e7010.

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