“Transcriptome analysis on Ion Proton™ Sequencer” Stephan Gunther (MPI Bad Nauheim) Ion World Tour Berlin

Stephan Gunther works on cardiac stem cell transcriptomics, as well as using de-novo transcriptome assembly of non-model organisms from Ion Proton™ data.

In this video interview he discusses the use of size selection in their library preparation, as well as steps they took to reduce the number of PCR duplicates from small input amounts.