CRISPR-Cas9 is rapidly evolving as the tool of choice for genome editing in mammalian cells. The delivery of Cas9 and synthesis of guide RNA (gRNA) remain as steps that limit overall efficiency and general ease of use.
During his presentation at ESHG 2015, Jason Potter of Thermo Fisher Scientific:
– Described novel methods for rapid synthesis of gRNA and highly efficient delivery of Cas9 protein/gRNA complexes into a variety of cells
– Showed data using lentivirus-based CRISPR delivery for high-throughput screening of mammalian cell populations
– Demonstrated how the rapid evolution of editing tools for mammalian genomes has resulted in these 2 CRISPR-based platforms by simplifying the cell engineering workflow and providing a pre-designed, ready to use platform for efficient compound screening in mammalian cell lines