{"id":10246,"date":"2017-03-08T03:40:22","date_gmt":"2017-03-08T03:40:22","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=10246"},"modified":"2017-03-13T20:18:26","modified_gmt":"2017-03-13T20:18:26","slug":"traditional-cloning-versus-gene-synthesis","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/traditional-cloning-versus-gene-synthesis\/","title":{"rendered":"Traditional Cloning versus Gene Synthesis"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2017\/03\/blog20hero201600x35020gns-1.png\" \/><\/p>\n<p><em>It\u2019s never been easier to make DNA!\u00a0 <\/em>Generating an expression construct is typically the first step in your protein expression workflow. Obviously there are many ways to get the desired expression plasmid. One of them is <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307gns\" target=\"_blank\">synthetic gene synthesis<\/a>, which is becoming more popular in today\u2019s research labs. There are several good reasons to synthetically make your custom DNA:<\/p>\n<ul>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Freedom of design (e.g. add\/remove restriction sites to your sequence)<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 No physical template required<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Ability to optimize your sequence for improved protein expression<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Fast access to your DNA (production times starting from <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/geneart-gene-synthesis\/geneart-gene-synthesis-rush-services.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307rush\" target=\"_blank\">5 business days<\/a>)<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Utilizing freed up time for other research<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Economical with respect to money and time<\/li>\n<\/ul>\n<div style=\"width: 905px\" class=\"wp-caption alignmiddle\"><img loading=\"lazy\" decoding=\"async\" style=\"vertical-align: middle\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2017\/03\/geneart20gene20synthesis20versus20classic20cloning-1.jpg\" width=\"895\" height=\"537\" \/><p class=\"wp-caption-text\">Figure 1. Workflow comparison of GeneArt Gene Synthesis versus classic cloning<\/p><\/div>\n<p>&nbsp;<\/p>\n<p>There are several providers of synthetic genes in the market place, but <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/geneart-gene-synthesis.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307gnschild\" target=\"_blank\">Invitrogen\u2122 GeneArt\u2122 Gene Synthesis<\/a>\u00a0and services provides additional <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/value-of-dna-project.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307value\" target=\"_blank\">value beyond just the DNA<\/a>:<\/p>\n<ul>\n<li>\u00a0 \u00a0 \u00a0 \u00a0\u00a0<strong>Dependability<\/strong>: More than 17 years\u2019 experience in making synthetic DNA; trusted Invitrogen quality provided by ISO certified quality systems; and sustainability in production<\/li>\n<li>\u00a0 \u00a0 \u00a0 \u00a0\u00a0<strong>Optimized performance and cost-effectiveness<\/strong>: Optimize your sequence for reliable and increased expression in your host system with our proprietary <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/geneart-gene-synthesis\/geneoptimizer.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307optimize\" target=\"_blank\">GeneOptimizer\u2122<\/a> software and save on cost per mg protein. <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/geneart-gene-synthesis\/geneoptimizer.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307optimize\" target=\"_blank\">It\u2019s proven to work!<\/a><\/li>\n<li>\u00a0 \u00a0 \u00a0 \u00a0\u00a0<strong>Customer Experience<\/strong>:\u00a0 It\u2019s never been easier to order synthetic DNA using the online <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/geneart-gene-synthesis\/geneart-portal-quickstart-guide.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307quickstart\" target=\"_blank\">GeneArt\u2122 Portal<\/a> at any time and any place. No need to wait for a quote or optimization results. Just upload your sequence, specify your requirements, and place your order. That\u2019s it. Our experienced customer care group is your convenient single point of contact for all of your gene synthesis inquiries.<\/li>\n<li>\u00a0 \u00a0 \u00a0 \u00a0\u00a0<strong>The product of your needs<\/strong>: Our synthetic DNA portfolio includes several offers according to your needs and budget. Individually design your project. Starting from <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/gene-strings-dna-fragments.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307strings\" target=\"_blank\">Invitrogen\u2122 GeneArt\u2122 Strings\u2122 DNA Fragments<\/a> to expression ready constructs, DNA libraries, and protein expression and purification services. All in-house, all from one provider.<\/li>\n<\/ul>\n<p>Find out more about the added value of GeneArt Gene Synthesis <a href=\"http:\/\/bit.ly\/2lW9f39\" target=\"_blank\">here<\/a>.<\/p>\n<p>Read what customers say about our products and services <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/products-and-services\/promotions\/geneart-it-customer-stories.html?cid=bid_SB_GNS_cloningVSgns_blog_20170307cs\" target=\"_blank\">here<\/a>.<\/p>\n<p>For Research Use Only. Not for use in diagnostic procedures.<\/p>\n<p>&nbsp;<\/p>\n<p>Sources:<\/p>\n<p>http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/cloning\/gene-synthesis\/geneart-gene-synthesis.html<\/p>\n","protected":false},"excerpt":{"rendered":"<p>It\u2019s never been easier to make DNA!\u00a0 Generating an expression construct is typically the first step in your protein expression workflow. Obviously there are many ways to get the desired expression plasmid. One of them is synthetic gene synthesis, which is becoming more popular in today\u2019s research labs. There are several good reasons to synthetically<\/p>\n","protected":false},"author":120,"featured_media":10249,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[101],"tags":[],"division":[],"class_list":{"0":"post-10246","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-product-news","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Traditional Cloning versus Gene Synthesis - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/traditional-cloning-versus-gene-synthesis\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Traditional Cloning versus Gene Synthesis\" \/>\n<meta property=\"og:description\" content=\"It\u2019s never been easier to make DNA!\u00a0 Generating an expression construct is typically the first step in your protein expression workflow. 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These complex structures span cell membranes, performing vital functions from signal transduction to transport of molecules across cellular barriers. Low expression yields, protein misfolding, and aggregation frequently plague researchers attempting to produce functional transmembrane proteins. 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The A260\/A280 ratio can indicate potential protein contamination, while the A260\/A230 ratio can reveal residual salts, phenol, guanidine compounds, and other extraction-related contaminants. 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