{"id":10805,"date":"2017-09-06T15:37:34","date_gmt":"2017-09-06T15:37:34","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=10805"},"modified":"2017-09-06T17:17:36","modified_gmt":"2017-09-06T17:17:36","slug":"the-next-generation-of-pcr-enzymes-purity-accuracy-with-dna-free-pcr-enzymes","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/the-next-generation-of-pcr-enzymes-purity-accuracy-with-dna-free-pcr-enzymes\/","title":{"rendered":"The Next Generation of PCR Enzymes: Purity and accuracy with DNA-free PCR enzymes"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" style=\"margin-top: 2px;margin-bottom: 2px;vertical-align: middle\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2017\/09\/blue201600x583.jpg\" width=\"1056\" height=\"385\" \/><\/p>\n<p>Molecular diagnostics, a driving force in health care, relies heavily on tests utilizing Polymerase Chain Reaction (PCR) technology. PCR enables sensitive detection, specific identification, as well as accurate quantification of disease causing agents. To avoid false positives and uncertainty in the assay results, it is crucial that the reagents used in any PCR test are free of foreign DNA; such as DNA originating from the manufacturing environment or human operator, the host DNA of recombinant protein-expressing cells, or carry over DNA from shared manufacturing equipment.<\/p>\n<div style=\"width: 130px\" class=\"wp-caption alignright\"><a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/about-us\/partnering-licensing\/oem-commercial-supply\/life-science-tools\/pcr-enzymes-rt-commercial-supply\/dna-free-reagents.html?icid=BID_MBO_DNAFreeWP_Blog1_20170905\"><img loading=\"lazy\" decoding=\"async\" style=\"border: 1px solid black;float: right;margin-left: 5px;margin-right: 5px\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2017\/09\/wp20image.jpg\" alt=\"\" width=\"120\" height=\"120\" \/><\/a><p class=\"wp-caption-text\">DNA-Free White Paper<\/p><\/div>\n<p>Thermo Fisher Scientific has developed a new manufacturing paradigm that delivers a much purer PCR reagent enabling better diagnostic assays. This new process\u00a0to manufacture DNA-free enzymes incorporate\u00a0\u00a0a closed and single-use system based technology to remove the risk of DNA contamination inherent to the conventional manufacturing process<sup>1<\/sup>.\u00a0To ensure conformance to strict purity requirements expected by molecular diagnostics developers,\u00a0we subject our DNA-free PCR reagents to stringent quality tests to ensure reagents are free of contaminating bacterial, human and plasmid-born DNA.\u00a0\u00a0This combination of manufacturing systems and\u00a0quality testing delivers PCR reagents that are 100X cleaner than other DNA-free reagents on the market.<\/p>\n<p>For more information about single-use system manufacturing of DNA-free PCR enzymes, download the <a href=\"http:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/single-use-technology-dna-free-pcr-reagents-white-paper.pdf?icid=BID_MBO_DNAFreeWP_Blog1_20170905\" target=\"_blank\" rel=\"noopener\">white paper<\/a>.<\/p>\n<p><em>For Research Use Only.\u00a0 Not for use in diagnostic procedures<\/em>.<\/p>\n<p><sup>1<\/sup><span style=\"font-size: 8pt\">Spangler R, Goddard NL, Thaler DS (2009) Optimizing <\/span><em style=\"font-size: 8pt\">Taq <\/em><span style=\"font-size: 8pt\">polymerase concentration for improved signal-to-noise in the broad-range detection of low-abundance bacteria. <\/span><em style=\"font-size: 8pt\">PLoS One <\/em><span style=\"font-size: 8pt\">4:e70<\/span><span style=\"font-size: 8pt\">10.<\/span><\/p>\n<p><!-- BEGIN KAPOST ANALYTICS CODE --><\/p>\n<p><!-- var _kaq = _kaq || []; _kaq.push([2, \"59a9e21063de3a0026000694\", \"5674227b963491538f00011d\"]); (function(){ var ka = document.createElement(\"script\"); ka.async=true; ka.id=\"ka_tracker\"; ka.src = (document.location.protocol == \"https:\" ? \"https\" : \"http\") + \":\/\/analytics.kapost.com\/ka.js\"; var s = document.getElementsByTagName(\"script\")[0]; s.parentNode.insertBefore(ka, s); })(); \/\/--><\/p>\n<p><!-- END KAPOST ANALYTICS CODE --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Molecular diagnostics, a driving force in health care, relies heavily on tests utilizing Polymerase Chain Reaction (PCR) technology. PCR enables sensitive detection, specific identification, as well as accurate quantification of disease causing agents. To avoid false positives and uncertainty in the assay results, it is crucial that the reagents used in any PCR test are<\/p>\n","protected":false},"author":120,"featured_media":10804,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[172,101],"tags":[245,239,244,240,246,243,247,44,242,241],"division":[],"class_list":{"0":"post-10805","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-general","8":"category-product-news","9":"tag-dna-contamination","10":"tag-dna-free","11":"tag-dna-free-enzymes","12":"tag-dna-free-reagents","13":"tag-molecular-diagnostics","14":"tag-pcr-enzymes","15":"tag-pcr-reagents","16":"tag-plasmid","17":"tag-single-use-system","18":"tag-sus","19":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>The Next Generation of PCR Enzymes: Purity and accuracy with DNA-free PCR enzymes - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/the-next-generation-of-pcr-enzymes-purity-accuracy-with-dna-free-pcr-enzymes\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The Next Generation of PCR Enzymes: Purity and accuracy with DNA-free PCR enzymes\" \/>\n<meta property=\"og:description\" content=\"Molecular diagnostics, a driving force in health care, relies heavily on tests utilizing Polymerase Chain Reaction (PCR) technology. 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