{"id":10949,"date":"2017-11-29T11:34:59","date_gmt":"2017-11-29T11:34:59","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=10949"},"modified":"2017-11-29T22:35:53","modified_gmt":"2017-11-29T22:35:53","slug":"getting-more-answers-from-challenging-bone-samples","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/getting-more-answers-from-challenging-bone-samples\/","title":{"rendered":"Getting More Answers From Challenging Bone Samples"},"content":{"rendered":"<p class=\"Pa5\"><img loading=\"lazy\" decoding=\"async\" class=\" wp-image-10951 alignleft\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-1.png\" alt=\"\" width=\"321\" height=\"206\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-1.png 514w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-1-300x193.png 300w\" sizes=\"auto, (max-width: 321px) 100vw, 321px\" \/><\/p>\n<p class=\"Pa5\">Bone samples are one of the most difficult sample types encountered in forensic laboratories. The extracted DNA is often degraded and obtained in low quantity, making it difficult to obtain usable <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/pcr-amplification-forensic-dna-profiling.html?icid=GSD_blog_hid_bone-samples\">STR profiles<\/a>. Also, the age, amount, and condition of bone samples present challenges for processing. Older bones (&gt;20 years) are problematic samples for low DNA yield, but the storage condition of even contemporary bones (&lt;20 years) can present challenges depending on the crime scene condition and environmental exposure of the bones. Labs are now able to process samples with an improved likelihood of achieving results with easy-to-use tools and a reduction in workflow steps.<\/p>\n<p class=\"Default\"><a href=\"http:\/\/assets.thermofisher.com\/TFS-Assets\/GSD\/Application-Notes\/get-more-answers-from-challenging-bone-samples-app-note.pdf?icid=GSD_blog_hid_bone-samples\">Read the app note<\/a> where we present data from three forensic laboratories that demonstrate high-quality STR results with increased allele recovery from bones.<\/p>\n<p class=\"Default\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-10952 alignright\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-2.png\" alt=\"\" width=\"579\" height=\"337\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-2.png 975w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-2-300x174.png 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-2-768x447.png 768w\" sizes=\"auto, (max-width: 579px) 100vw, 579px\" \/><\/p>\n<p class=\"Pa5\">These labs used the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/sample-preparation-extraction\/prepfiler-prepfiler-bta-forensic-dna-extraction-kits.html?icid=GSD_blog_hid_bone-samples\">PrepFiler BTA Forensic DNA Extraction Kit<\/a>, the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/dna-quantification-solutions.html?icid=GSD_blog_hid_bone-samples\">Quantifiler Trio DNA Quantification Kit<\/a> to assess the quality and quality of the extracted DNA, and amplification using the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/pcr-amplification-forensic-dna-profiling\/globalfiler.html?icid=GSD_blog_hid_bone-samples\">GlobalFiler PCR Amplification Kit<\/a> and <a href=\"http:\/\/www.thermofisher.com\/es\/es\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/pcr-amplification-forensic-dna-profiling\/ngm-detect-pcr-amplification-kit.html?icid=GSD_blog_hid_bone-samples\">NGM Detect DNA Amplification Kit<\/a> with these highly compromised or low copy number samples.<\/p>\n<p><!-- BEGIN KAPOST ANALYTICS CODE --><\/p>\n<p><!-- var _kaq = _kaq || []; _kaq.push([2, \"5a1f2c5d2bcc2d00b500008c\", \"5674227b963491538f00011d\"]); (function(){ var ka = document.createElement(\"script\"); ka.async=true; ka.id=\"ka_tracker\"; ka.src = (document.location.protocol == \"https:\" ? \"https\" : \"http\") + \":\/\/analytics.kapost.com\/ka.js\"; var s = document.getElementsByTagName(\"script\")[0]; s.parentNode.insertBefore(ka, s); })(); \/\/--><\/p>\n<p><!-- END KAPOST ANALYTICS CODE --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Bone samples are one of the most difficult sample types encountered in forensic laboratories. The extracted DNA is often degraded and obtained in low quantity, making it difficult to obtain usable STR profiles. Also, the age, amount, and condition of bone samples present challenges for processing. Older bones (&gt;20 years) are problematic samples for low<\/p>\n","protected":false},"author":115,"featured_media":10951,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[71],"tags":[],"division":[],"class_list":{"0":"post-10949","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-forensics","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Getting More Answers From Challenging Bone Samples - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/getting-more-answers-from-challenging-bone-samples\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Getting More Answers From Challenging Bone Samples\" \/>\n<meta property=\"og:description\" content=\"Bone samples are one of the most difficult sample types encountered in forensic laboratories. The extracted DNA is often degraded and obtained in low quantity, making it difficult to obtain usable STR profiles. Also, the age, amount, and condition of bone samples present challenges for processing. Older bones (&gt;20 years) are problematic samples for low\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/getting-more-answers-from-challenging-bone-samples\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2017-11-29T11:34:59+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2017-11-29T22:35:53+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2017\/11\/Bone-samples-blog-1.png\" \/>\n\t<meta property=\"og:image:width\" content=\"514\" \/>\n\t<meta property=\"og:image:height\" content=\"330\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Angie Lackey\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Angie Lackey\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/\"},\"author\":{\"name\":\"Angie Lackey\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/aee4ecb3303ff14eb0ffd6f4b0554fab\"},\"headline\":\"Getting More Answers From Challenging Bone Samples\",\"datePublished\":\"2017-11-29T11:34:59+00:00\",\"dateModified\":\"2017-11-29T22:35:53+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/\"},\"wordCount\":184,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2017\\\/11\\\/Bone-samples-blog-1.png\",\"articleSection\":[\"Forensics\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/getting-more-answers-from-challenging-bone-samples\\\/\",\"name\":\"Getting More Answers From Challenging Bone Samples - 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Featuring perspectives from Ray Ketchum, PhD; Sarah Philo,\u2026","rel":"","context":"In &quot;Lab Tips and Tricks&quot;","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Ketchum-headshot.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Ketchum-headshot.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Ketchum-headshot.jpg?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":19943,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/dna-and-rna-purity-ratios-a260-a280-and-a260-a230-explained\/","url_meta":{"origin":10949,"position":1},"title":"DNA and RNA Purity Ratios: A260\/A280 and A260\/A230 Explained","author":"Marlene Gasdia-Cochrane","date":"September 1, 2026","format":false,"excerpt":"Overview DNA and RNA purity ratios help researchers assess nucleic acid sample quality before downstream analysis. The A260\/A280 ratio can indicate potential protein contamination, while the A260\/A230 ratio can reveal residual salts, phenol, guanidine compounds, and other extraction-related contaminants. For a more complete assessment, purity ratios should be evaluated alongside\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"mRNA Technology - Messenger RNA - Two Strands of mRNA on Abstract Technology Background - Development of New Therapies and Vaccines Based on mRNA Technology - Conceptual Illustration","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-1366552805_mrna.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-1366552805_mrna.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-1366552805_mrna.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-1366552805_mrna.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-1366552805_mrna.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-1366552805_mrna.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19885,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-mix-dna-templates\/","url_meta":{"origin":10949,"position":2},"title":"Advancing the Gold Standard: Faster, Smarter, and Built for Challenging Templates","author":"Behind The Bench Staff","date":"August 5, 2026","format":false,"excerpt":"Sanger sequencing has long been the gold standard for accuracy, trusted by laboratories worldwide for applications ranging from plasmid verification to confirming next-generation sequencing results. Yet as research evolves, so do the demands placed on sequencing workflows. Today\u2019s labs are not only looking for accuracy\u2014they need speed, flexibility, and reliable\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"DNA sequencing sample tube with DNA helix and sequencing chromatogram representing Sanger sequencing workflow.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19805,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/liquid-biopsy-workflow-standardization\/","url_meta":{"origin":10949,"position":3},"title":"Improving the Reliability of Liquid Biopsy in Real-World Settings Through Workflow Standardization: A Discussion with Dr. Matteo Allegretti","author":"Ashleigh Barlow","date":"June 18, 2026","format":false,"excerpt":"Liquid biopsy offers a powerful, noninvasive way to detect and track cancer, but ensuring reliability in real-world settings remains a key hurdle. Variability in workflows and the need for robust verification steps limit widespread adoption. Dr. Matteo Allegretti, Senior Researcher at the Translational Oncology Research Unit at the IRCCS Regina\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Research scientist operating laboratory equipment during liquid biopsy and biomarker research.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":19883,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/building-a-better-sample-qc-workflow-from-extraction-to-sequencing\/","url_meta":{"origin":10949,"position":4},"title":"Building a Better Sample QC Workflow: From Extraction to Sequencing","author":"Marlene Gasdia-Cochrane","date":"August 25, 2026","format":false,"excerpt":"Article Summary Successful genomics experiments don't begin with sequencing\u2014they begin with sample quality. Every downstream application, from PCR to next-generation sequencing (NGS), depends on starting with nucleic acids that are accurately quantified and free from contaminants that could compromise results. Establishing a consistent quality control workflow helps laboratories improve reproducibility\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"A pipette drops a liquid into a petri dish with a dna sequence background, science concept. It suggests medical or genetic research.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-2216731026_petridish-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-2216731026_petridish-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-2216731026_petridish-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-2216731026_petridish-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-2216731026_petridish-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/iStock-2216731026_petridish-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19640,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/direct-from-lysate-snp-genotyping-in-research-applications\/","url_meta":{"origin":10949,"position":5},"title":"Direct-from-Lysate SNP Genotyping in Research Applications","author":"Ashleigh Barlow","date":"April 6, 2026","format":false,"excerpt":"Direct-from-lysate workflow enabled by DNA Extract All Reagents compared to traditional DNA purification for SNP genotyping applications. Can You Perform SNP Genotyping Without DNA Purification? The short answer: In many research workflows, direct-from-lysate SNP genotyping can be performed without traditional DNA purification\u2014when appropriate chemistries and verification procedures are used. 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