{"id":11439,"date":"2018-07-14T00:32:50","date_gmt":"2018-07-14T00:32:50","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=11439"},"modified":"2018-07-14T00:44:11","modified_gmt":"2018-07-14T00:44:11","slug":"case-study-1-flexibility-makes-a-good-enzyme-package","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/case-study-1-flexibility-makes-a-good-enzyme-package\/","title":{"rendered":"CASE STUDY #1: Flexibility makes a good enzyme package"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" style=\"vertical-align: top\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2018\/07\/case20study20120hero.png\" width=\"1600\" height=\"550\" \/><\/p>\n<p><span style=\"font-size: 14pt\">A biotech company was developing a PCR-based assay to screen for the pathogens behind hundreds of infectious diseases.\u00a0 They found that DNA contamination from host cells in most commercially available <em>Taq<\/em> polymerase could lead to false findings as well as a need in performance requirement for their highly sensitive assays.\u00a0 After evaluating enzymes from several suppliers, they came to Thermo Fisher Scientific for custom solution to meet their performance criteria.\u00a0<\/span><\/p>\n<p><span style=\"font-size: 14pt\"><img loading=\"lazy\" decoding=\"async\" class=\"alignright\" style=\"float: right;border: 1px solid black;margin: 1px 5px 1px 5px\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2018\/07\/cs20120image20a.png\" width=\"380\" height=\"249\" \/><\/span><\/p>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/www.youtube.com\/watch?v=oBJW39hKRdg&amp;t=25s\">Watch the video from Todd Howren<\/a>, Commercial Supply Business Lead, describing the development of enzymes using a p<\/span><span style=\"font-size: 14pt\">ropriet<\/span><span style=\"font-size: 14pt\">ary <\/span><span style=\"font-size: 14pt\">technology called molecular <\/span><em style=\"font-size: 14pt\">in vitro<\/em><span style=\"font-size: 14pt\"> evolution.\u00a0 This technology enables the introduction and selection of multiple favorable mutations into a wild-type enzyme, resulting in performance improvements of enzyme function.\u00a0 With these engineered enzymes, molecular DNA-based assay design and development can be improve drastically and further differentiate for various pathogens and sample types.\u00a0<\/span><\/p>\n<p><span style=\"font-size: 14pt\"><strong>Interested in a custom solution?\u00a0 Contact us at MDxenzymes@thermofisher.com<\/strong><\/span><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><!-- BEGIN KAPOST ANALYTICS CODE --><\/p>\n<p><!-- var _kaq = _kaq || []; _kaq.push([2, \"5b494108da919c0027000065\", \"5674227b963491538f00011d\"]); (function(){ var ka = document.createElement(\"script\"); ka.async=true; ka.id=\"ka_tracker\"; ka.src = (document.location.protocol == \"https:\" ? \"https\" : \"http\") + \":\/\/analytics.kapost.com\/ka.js\"; var s = document.getElementsByTagName(\"script\")[0]; s.parentNode.insertBefore(ka, s); })(); \/\/--><\/p>\n<p><!-- END KAPOST ANALYTICS CODE --><\/p>\n","protected":false},"excerpt":{"rendered":"<p>A biotech company was developing a PCR-based assay to screen for the pathogens behind hundreds of infectious diseases.\u00a0 They found that DNA contamination from host cells in most commercially available Taq polymerase could lead to false findings as well as a need in performance requirement for their highly sensitive assays.\u00a0 After evaluating enzymes from several<\/p>\n","protected":false},"author":120,"featured_media":11438,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[172],"tags":[],"division":[],"class_list":{"0":"post-11439","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-general","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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