{"id":11474,"date":"2018-07-31T05:31:13","date_gmt":"2018-07-31T05:31:13","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=11474"},"modified":"2018-07-31T15:09:05","modified_gmt":"2018-07-31T15:09:05","slug":"case-study-2-commercializing-molecular-assay-kits","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/case-study-2-commercializing-molecular-assay-kits\/","title":{"rendered":"CASE STUDY #2: Commercializing Molecular Assay Kits?"},"content":{"rendered":"<p><img decoding=\"async\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/9\/2018\/07\/case20study20220hero201600x350-4.jpg\" \/><\/p>\n<p><span style=\"font-size: 14pt\">A biotech startup was developing a <a href=\"http:\/\/bit.ly\/2AnRF4P\" target=\"_blank\" rel=\"noopener\">PCR-based multiplex assays<\/a> for detection in infectious and inherited diseases.\u00a0 These assays will run on an instrument platform that utilizes disposable cartridge containing a lyophilized PCR assay.\u00a0The client approached us after they had completed development with our enzymes, which provided the performance that met their assay requirement.\u00a0 A major problem faced in lyophilized assay was that most enzymes on the market were incompatible with lyophilization due to presence of glycerol in the storage buffer. <\/span><\/p>\n<p><span style=\"font-size: 14pt\">Check out the video of Julia Fletcher, R&amp;D Program Lead; discussing how we enter into a R&amp;D and manufacturing development project with the\u00a0<\/span><span style=\"font-size: 14pt\">client to remove glycerol from our enzymes prior to lyophilization.\u00a0The project consisted of:<\/span><\/p>\n<ul>\n<li><span style=\"font-size: 14pt\">Development of a method in R&amp;D to remove glycerol from the enzymes\u00a0 \u00a0<\/span><\/li>\n<li><span style=\"font-size: 14pt\">Transfer of this method to manufacturing<\/span><\/li>\n<li><span style=\"font-size: 14pt\">Scaling and validation of the method in manufacturing<\/span><\/li>\n<\/ul>\n<p><span style=\"font-size: 14pt\">The collaboration with the client to co-develop and manufacture a custom product formulation allowed the client to bring their lyophilized assay to market. Download the &#8220;<a href=\"http:\/\/bit.ly\/2LEcC0y\" target=\"_blank\" rel=\"noopener\">Take your assay anywhere<\/a>&#8221; Case Study to learn more.\u00a0<\/span><\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/DPWKAghTbBM?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p><span style=\"font-size: 14pt\">Interested in a custom solution?\u00a0 Contact us at <\/span><a style=\"font-size: 14pt\" href=\"mailto:MDxenzymes@thermofisher.com\">MDxenzymes@thermofisher.com<\/a><\/p>\n<p><!-- BEGIN KAPOST ANALYTICS CODE --><\/p>\n<p><!-- var _kaq = _kaq || []; _kaq.push([2, \"5b5fdb3bacb5dc006300006d\", \"5674227b963491538f00011d\"]); (function(){ var ka = document.createElement(\"script\"); ka.async=true; ka.id=\"ka_tracker\"; ka.src = (document.location.protocol == \"https:\" ? \"https\" : \"http\") + 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major<\/p>\n","protected":false},"author":120,"featured_media":11485,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[172],"tags":[],"division":[],"class_list":{"0":"post-11474","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-general","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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As Influenza A viruses continue to\u2026","rel":"","context":"In &quot;Animal Health and AgriGenomics&quot;","block_context":{"text":"Animal Health and AgriGenomics","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/animal-health-and-agrigenomics\/"},"img":{"alt_text":"Chickens in a flock on a farm representing poultry populations monitored for avian influenza surveillance.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Untitled-3.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Untitled-3.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Untitled-3.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Untitled-3.png?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":19193,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/advancing-cancer-cell-based-assays-with-assay-ready-tumoroids\/","url_meta":{"origin":11474,"position":2},"title":"Advancing Cancer Cell-Based Assays with Assay-Ready Tumoroids","author":"Behind The Bench Staff","date":"October 28, 2025","format":false,"excerpt":"Cancer research is continually evolving, and patient-derived tumoroids are playing a crucial role in this progress. These three-dimensional (3D) cell culture models, also known as cancer organoids, closely mimic the genetic profile, gene expression patterns, and architecture of actual tumors. Therefore, tumoroids are increasingly being used for basic research, drug\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Microscopic image of HuLu051921 tumoroids stained with Hematoxylin and Eosin at 20x magnification, field 1, bottom section of the slide","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19271,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-qpcr-basics-guide\/","url_meta":{"origin":11474,"position":3},"title":"Multiplex vs. Singleplex qPCR: What do I need to know?","author":"Ashleigh Barlow","date":"December 3, 2025","format":false,"excerpt":"Real-time PCR (qPCR) has transformed how scientists detect and quantify nucleic acid targets, offering analytical sensitivity, speed, and scalability. Many real-time PCR reactions measure a single target per well \u2014 a setup called singleplex qPCR. However, the effectiveness of real-time PCR can be increased by an approach called multiplex qPCR.\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":19748,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/andes-virus-rt-qpcr-detection-workflow\/","url_meta":{"origin":11474,"position":4},"title":"Responding to Hantavirus: Advancing Rapid Molecular Detection","author":"Behind The Bench Staff","date":"May 14, 2026","format":false,"excerpt":"Article Summary Laboratories responding to the emerging 2026 Andes hantavirus incident need rapid, scalable molecular workflows that can integrate into existing RT-qPCR infrastructure. This workflow is best suited for public health laboratories, surveillance programs, academic research labs, and high-throughput testing facilities seeking rapid research-use-only (RUO) molecular detection capabilities. By combining\u2026","rel":"","context":"In &quot;Infectious Diseases&quot;","block_context":{"text":"Infectious Diseases","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/infectious-diseases\/"},"img":{"alt_text":"Illustration of hantavirus transmission showing rodent-derived viral particles entering the human respiratory system.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19606,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-qpcr-optimization-6-plex\/","url_meta":{"origin":11474,"position":5},"title":"Optimizing multiplex qPCR: How to amplify up to 6 targets without compromising analytical sensitivity","author":"Behind The Bench Staff","date":"April 1, 2026","format":false,"excerpt":"What happens when a multiplex qPCR assay begins to lose performance as additional targets are introduced? An assay that performs reproducibly in singleplex can exhibit Ct shift, reduced sensitivity, or compressed dynamic range when scaled to 5- or 6-plex. As plex level increases, competition among primers, probes, reporter dyes, and\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Graph comparing Ct values between singleplex and multiplex qPCR reactions showing \u0394Ct shift as targets increase.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/mulitplexing-image.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/mulitplexing-image.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/mulitplexing-image.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/mulitplexing-image.png?resize=700%2C400&ssl=1 2x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2018\/07\/case20study20220hero201600x350-4.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/11474","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=11474"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/11474\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media\/11485"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=11474"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=11474"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=11474"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=11474"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}