{"id":12440,"date":"2019-05-29T20:02:41","date_gmt":"2019-05-29T20:02:41","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=12440"},"modified":"2020-05-28T19:15:16","modified_gmt":"2020-05-28T19:15:16","slug":"gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/","title":{"rendered":"Gene expression applications for drug development at a Japanese pharmaceutical company"},"content":{"rendered":"

<\/p>\n

Recent advancements in next-generation sequencing (NGS) technology have contributed to developing a targeted approach for gene expression analysis, providing researchers with speed, scalability, and precision without the need for additional time and costly resources to get from samples to quality insights.<\/p>\n

We spoke with Dr. Ryo Fujii, Associate Director at the Japanese pharmaceutical company Axcelead Drug Discovery Partners, Inc., where he leads genomics, transcriptomics, and bioinformatics in the company\u2019s Integrated Biology Group. He uses gene expression analysis techniques with tissue cultures, animal models, and clinical samples as indices for investigating the mechanism of drug action and for confirming pharmacokinetics.<\/p>\n

When Dr. Fujii first performed gene expression analyses, he started with real-time PCR assays but felt limited by the gene types available. Now with the Ion AmpliSeq\u2122 Transcriptome Assay<\/a>, Dr. Fujii says that he is able to use both human and mouse genes, \u201cimproving both the quantity and quality of our gene analysis information.\u201d<\/p>\n

As little as 10 ng of total RNA required to perform transcriptome analysis<\/strong><\/p>\n

The Ion AmpliSeq Transcriptome Assay is part of an end-to-end NGS workflow solution that enables investigation of over 20,000 human or mouse genes using only a single assay.<\/p>\n

Dr. Fujii has relied on this assay for drug development since 2015. \u201cIt\u2019s very convenient because the assay requires only 10 ng of total RNA without additional steps required for removal of rRNA,\u201d he explains. He typically uses 96-well plates for his cell cultures and expose the cells to various types of drug candidates at different concentrations. With the Ion AmpliSeq Transcriptome Assay, Dr. Fujii and his team are able to get \u201cmore than enough RNA for the transcriptome analysis from a single well.\u201d<\/p>\n

Resulting data are reproducible, with a straightforward data analysis workflow<\/strong><\/p>\n

With the Ion Proton\u2122 System<\/a>, Dr. Fujii\u2019s team is able to easily process 16 samples from two sequencing reactions per day. He has found that the data generated from the Ion AmpliSeq Transcriptome Assay with NGS are reproducible using Applied Biosystems\u2122 TaqMan\u00ae Assays. Dr. Fujii recalls a time when he was \u201cusing the same positive control sample in different experiments, and the positive control data were so close to the actual data, that I thought I was looking at the wrong data.\u201d<\/p>\n

Dr. Fujii has also discovered that \u201cthe dynamic range of the Ion AmpliSeq Transcriptome Assay is greater than that of microarray. Because the Ion AmpliSeq Transcriptome Assay<\/a> sequences one target site per gene, data analysis is simpler due to having a resulting data set smaller when compared to traditional RNA-Seq.\u201d In addition to the simplicity of having a smaller data set that is reproducible, Dr. Fujii\u2019s team can analyze the resulting data easily with optimized plug-in software specifically created for use with this workflow, and access support from the knowledgeable technical support team at Thermo Fisher Scientific to help make data analysis straightforward.<\/p>\n

Future applications of transcriptome analysis for drug development<\/strong><\/p>\n

As for future applications of transcriptome analysis, Dr. Fujii believes he may be able to use it to assess off-target effects in oligonucleotide therapeutics. During the development of oligonucleotide drugs, he selects unique sequences from the human genome to form antisense DNA, which interact with the target. However, when the drug is applied to cell or model animals, unexpected off-target effects or disruption of cell signaling pathways occur. According to Dr. Fujii, transcriptome analysis could then, in theory, be used as a tool for \u201cconfirmation of off-target effects during early-stage drug development, and provide more efficiency in the development process.\u201d<\/p>\n

Accelerate drug development with an integrated drug discovery service<\/strong><\/p>\n

Dr. Fujii believes that successful drug development won\u2019t come from knowledge and understanding in only a single field. Instead, he feels strongly that integration with other related areas is key. Guided by this perspective, the first stage of Axcelead\u2019s integrative service was involved in providing high-throughput screening using the largest compound library in Japan. Because Axcelead Drug Discovery Partners is owned by Takeda Pharmaceutical Company, Dr. Fujii has been able to access a vast amount of drug development data accumulated from across the entire company. Using this approach, Dr. Fujii has been able to quickly identify chemical compounds with desired biological activities in a relatively short time, and with a high success rate.<\/p>\n

Using innovative technologies like the Ion AmpliSeq Transcriptome Assay, Dr. Fujii plans to continue in the development of novel approaches within the drug discovery and health care space by leveraging his team\u2019s collective expertise, cultivated through their extensive experience in drug development.<\/p>\n

Find out more about targeted human and mouse transcriptome sequencing at thermofisher.com\/ampliseqtranscriptome<\/strong><\/a><\/p>\n

 <\/p>\n

About Axcelead Drug Discovery Partners Inc.<\/strong><\/p>\n

Axcelead is the first comprehensive integrated drug discovery solutions provider in the pharmaceutical industry in Japan, having succeeded some portion of the drug discovery research department of Takeda Pharmaceutical Company Limited, and started its business in July 2017. Axcelead performs all of the functions related to nonclinical drug discovery research, drawing on the scientific powerhouse of their staff\u2019s expertise in a range of therapeutic areas at Takeda. Axcelead offers a one-stop shop for research services with a comprehensive set of best-in-class capabilities that meet a range of customer needs to bridge basic and applied clinical research\u2014from exploratory research to optimizing compounds.<\/p>\n

<\/p>\n

<\/p>\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

Recent advancements in next-generation sequencing (NGS) technology have contributed to developing a targeted approach for gene expression analysis, providing researchers with speed, scalability, and precision without the need for additional time and costly resources to get from samples to quality insights. We spoke with Dr. Ryo Fujii, Associate Director at the Japanese pharmaceutical company Axcelead<\/p>\n","protected":false},"author":120,"featured_media":12446,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[54],"tags":[43],"division":[],"class_list":{"0":"post-12440","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cancer-research","8":"tag-pcr","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nGene expression applications for drug development at a Japanese pharmaceutical company - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Gene expression applications for drug development at a Japanese pharmaceutical company\" \/>\n<meta property=\"og:description\" content=\"Recent advancements in next-generation sequencing (NGS) technology have contributed to developing a targeted approach for gene expression analysis, providing researchers with speed, scalability, and precision without the need for additional time and costly resources to get from samples to quality insights. We spoke with Dr. Ryo Fujii, Associate Director at the Japanese pharmaceutical company Axcelead\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2019-05-29T20:02:41+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-28T19:15:16+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png\" \/>\n\t<meta property=\"og:image:width\" content=\"865\" \/>\n\t<meta property=\"og:image:height\" content=\"574\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Behind The Bench Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Behind The Bench Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"4 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/\"},\"author\":{\"name\":\"Behind The Bench Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"headline\":\"Gene expression applications for drug development at a Japanese pharmaceutical company\",\"datePublished\":\"2019-05-29T20:02:41+00:00\",\"dateModified\":\"2020-05-28T19:15:16+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/\"},\"wordCount\":881,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2019\\\/05\\\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png\",\"keywords\":[\"PCR\"],\"articleSection\":[\"Cancer Research\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/\",\"name\":\"Gene expression applications for drug development at a Japanese pharmaceutical company - Behind the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2019\\\/05\\\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png\",\"datePublished\":\"2019-05-29T20:02:41+00:00\",\"dateModified\":\"2020-05-28T19:15:16+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2019\\\/05\\\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2019\\\/05\\\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png\",\"width\":865,\"height\":574},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Gene expression applications for drug development at a Japanese pharmaceutical company\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\",\"name\":\"Behind the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\",\"name\":\"Behind The Bench Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"caption\":\"Behind The Bench Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/behind-the-bench-staff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Gene expression applications for drug development at a Japanese pharmaceutical company - Behind the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/","og_locale":"en_US","og_type":"article","og_title":"Gene expression applications for drug development at a Japanese pharmaceutical company","og_description":"Recent advancements in next-generation sequencing (NGS) technology have contributed to developing a targeted approach for gene expression analysis, providing researchers with speed, scalability, and precision without the need for additional time and costly resources to get from samples to quality insights. We spoke with Dr. Ryo Fujii, Associate Director at the Japanese pharmaceutical company Axcelead","og_url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/","og_site_name":"Behind the Bench","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2019-05-29T20:02:41+00:00","article_modified_time":"2020-05-28T19:15:16+00:00","og_image":[{"width":865,"height":574,"url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png","type":"image\/png"}],"author":"Behind The Bench Staff","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Behind The Bench Staff","Est. reading time":"4 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/"},"author":{"name":"Behind The Bench Staff","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"headline":"Gene expression applications for drug development at a Japanese pharmaceutical company","datePublished":"2019-05-29T20:02:41+00:00","dateModified":"2020-05-28T19:15:16+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/"},"wordCount":881,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png","keywords":["PCR"],"articleSection":["Cancer Research"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/","url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/","name":"Gene expression applications for drug development at a Japanese pharmaceutical company - Behind the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png","datePublished":"2019-05-29T20:02:41+00:00","dateModified":"2020-05-28T19:15:16+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png","contentUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png","width":865,"height":574},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/gene-expression-applications-for-drug-development-at-a-japanese-pharmaceutical-company\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/behindthebench\/"},{"@type":"ListItem","position":2,"name":"Gene expression applications for drug development at a Japanese pharmaceutical company"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/behindthebench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c","name":"Behind The Bench Staff","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","caption":"Behind The Bench Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"}]}},"taxonomy_info":{"category":[{"value":54,"label":"Cancer Research"}],"post_tag":[{"value":43,"label":"PCR"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png",760,504,false],"author_info":{"display_name":"Behind The Bench Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"},"comment_info":0,"category_info":[{"term_id":54,"name":"Cancer Research","slug":"cancer-research","term_group":0,"term_taxonomy_id":54,"taxonomy":"category","description":"","parent":0,"count":143,"filter":"raw","meta":[],"cat_ID":54,"category_count":143,"category_description":"","cat_name":"Cancer Research","category_nicename":"cancer-research","category_parent":0}],"tag_info":[{"term_id":43,"name":"PCR","slug":"pcr","term_group":0,"term_taxonomy_id":43,"taxonomy":"post_tag","description":"","parent":0,"count":142,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":19810,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/integration-site-analysis-gene-therapy-safety\/","url_meta":{"origin":12440,"position":0},"title":"Genotoxicity, vectors, and the power of integration site analysis","author":"Ashleigh Barlow","date":"June 23, 2026","format":false,"excerpt":"Article Summary In this episode of Absolute Gene-ius, Michael Stump, PhD, President of ProtaGene, and Matteo Franco, Lead Scientist at ProtaGene, discuss how integration site analysis is helping researchers evaluate genotoxicity risks in cell and gene therapy workflows. Their conversation explores how viral vectors integrate into the genome, why integration\u2026","rel":"","context":"In "Gene Editing"","block_context":{"text":"Gene Editing","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/gene-editing\/"},"img":{"alt_text":"Matteo Franco, Lead Scientist at ProtaGene, discussing integration site analysis and genotoxicity assessment","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19976,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/taqman-assay-design-genomic-data\/","url_meta":{"origin":12440,"position":1},"title":"What does it take to keep 20 million Applied Biosystems TaqMan Assays current?","author":"Ashleigh Barlow","date":"September 30, 2026","format":false,"excerpt":"How do you keep more than 20 million TaqMan assays current as genomic knowledge evolves? Every year, our understanding of biology becomes more complete. New variants are discovered, transcript annotations improve, gene models evolve, and our assay-design algorithms advance. That does not mean an assay designed years ago is suddenly\u2026","rel":"","context":"In "Bioinformatics"","block_context":{"text":"Bioinformatics","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/bioinformatics\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/BAN-16090146_1528x860.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/BAN-16090146_1528x860.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/BAN-16090146_1528x860.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/BAN-16090146_1528x860.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/BAN-16090146_1528x860.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/BAN-16090146_1528x860.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19860,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/choose-sybr-green-qpcr-master-mix\/","url_meta":{"origin":12440,"position":2},"title":"Choosing the Right SYBR Green Master Mix for Your Research and Budget","author":"Behind The Bench Staff","date":"July 28, 2026","format":false,"excerpt":"Every qPCR experiment begins with a series of decisions. How many targets should you analyze? How many samples and replicates are enough? Should you expand your sample cohort or reserve budget for downstream analytical validation studies? The answers depend not only on the scientific question, but also on the resources\u2026","rel":"","context":"In "Lab Tips and Tricks"","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"Research scientist preparing samples for a real-time PCR experiment in a molecular biology laboratory.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19435,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/scaling-human-relevant-oncology-testing-oncopro-tumoroids-on-screenin3ds-upscale-platform\/","url_meta":{"origin":12440,"position":3},"title":"Scaling Human-Relevant Oncology Testing: OncoPro Tumoroids on ScreenIn3D\u2019s UpScale Platform","author":"Behind The Bench Staff","date":"February 25, 2026","format":false,"excerpt":"The United States National Institutes of Health has prioritized human cell-based in vitro approaches to \u201cmodel human disease and capture human variability and patient-specific characteristics,\u201d complementing the U.S. Food and Drug Administration\u2019s goals to use advanced in vitro assays as a New Approach Methodology (NAM) that could reduce animal testing.\u2026","rel":"","context":"In "Cancer Research"","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19658,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/is-raman-spectroscopy-a-better-verification-method-in-pharmaceutical-manufacturing\/","url_meta":{"origin":12440,"position":4},"title":"Is Raman Spectroscopy a Better Verification Method in Pharmaceutical Manufacturing?","author":"Marlene Gasdia-Cochrane","date":"May 5, 2026","format":false,"excerpt":"Article Summary Verification testing is a critical step in pharmaceutical manufacturing, helping to ensure that every product released meets strict safety, quality, and regulatory standards. Verification helps ensure that products contain the correct active ingredients, meet concentration specifications, are free from contamination, and maintain structural and chemical integrity. This process\u2026","rel":"","context":"In "Pharma & Biotech Manufacturing"","block_context":{"text":"Pharma & Biotech Manufacturing","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/pharmamfg\/"},"img":{"alt_text":"Yellow pill and red check mark sitting on brown wood surface in front of abstract defocused background. Horizontal composition with selective focus and copy space. Front view. Drug approval concept.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/iStock-1223991858.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/iStock-1223991858.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/iStock-1223991858.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/iStock-1223991858.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/iStock-1223991858.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/04\/iStock-1223991858.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19223,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/innovations-in-qpcr-technology\/","url_meta":{"origin":12440,"position":5},"title":"Innovations in qPCR Technology: What Researchers Need to Know","author":"Ashleigh Barlow","date":"October 29, 2025","format":false,"excerpt":"https:\/\/youtu.be\/z9Kb1xAnNcg?si=TVUIc8rUxZUOhCqA Real-time PCR, also known as quantitative polymerase chain reaction (qPCR) technology, has been a cornerstone of molecular biology research for decades. With continuous advancements, qPCR remains a critical tool for researchers worldwide. Damien Luk, Senior Director of Product Management at Thermo Fisher Scientific, shared his insights on the future\u2026","rel":"","context":"In "Developmental Biology"","block_context":{"text":"Developmental Biology","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/developmental-biology\/"},"img":{"alt_text":"A lineup of Thermo Fisher Scientific QuantStudio real-time PCR systems, including QuantStudio 3, 5, 6 Pro, 7 Pro, and 12K Flex instruments, displayed against a blue gradient background.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2019\/05\/pg2008-pjt4739-col09437-ro_review_-_blog_for_axcelead_mouse_transcriptome_-_global-qc-gr-clean-3.png","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/12440","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=12440"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/12440\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media\/12446"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=12440"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=12440"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=12440"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=12440"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}