{"id":13136,"date":"2020-01-28T21:09:28","date_gmt":"2020-01-28T21:09:28","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=13136"},"modified":"2021-03-22T17:35:11","modified_gmt":"2021-03-22T17:35:11","slug":"improving-dna-profiling","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/improving-dna-profiling\/","title":{"rendered":"Forensic DNA Profiling"},"content":{"rendered":"<h2><em>Improving The Sexual Assault Workflow Using Applied Biosystems Forensic Solutions<\/em><\/h2>\n<p>The Institute of Environmental Science and Research Limited \u00a0(ESR) supports the New Zealand Police in crime scene investigation, evidence collection and forensic DNA profiling. The DNA team has optimized their analysis workflow to gain the most probative information possible, especially from sexual assault evidence kits.<\/p>\n<p><iframe loading=\"lazy\" title=\"Applied Biosystems Quantifiler Trio, Yfiler Plus and Identifiler kits\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/Wn-tPdWOfhE?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p><a href=\"https:\/\/www.youtube.com\/watch?v=Wn-tPdWOfhE\">In this video<\/a>, \u00a0Donna Foskin, senior scientist at ESR, describes how they process sexual assault kits. To start, the details of the case are taken into consideration along with the length of time that has passed since the assault occurred. Once the DNA has been extracted, the team uses <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/dna-quantification-solutions.html\">Applied Biosystems Quantifiler Trio kit<\/a> to assess the quantity of DNA present along with the male-to-female ratio. This information then guides the decision to use autosomal STR testing with <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/pcr-amplification-forensic-dna-profiling.html\">Applied Biosystems Identifiler PCR Amplification Kit<\/a> or Y-STR testing with Applied Biosystems <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/pcr-amplification-forensic-dna-profiling\/y-str-analysis.html\">Yfiler Plus PCR Amplification Kit<\/a> or both.<\/p>\n<p><a href=\"https:\/\/www.youtube.com\/watch?v=Wn-tPdWOfhE\">Watch the video<\/a> to get all the details on how ESR has improved their processes to gain a better understanding of their more complex cases.<\/p>\n<p style=\"text-align: right\"><em>For Research Forensic or Paternity Use Only. Not for use in diagnostic procedures.\u00a0<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Improving The Sexual Assault Workflow Using Applied Biosystems Forensic Solutions The Institute of Environmental Science and Research Limited \u00a0(ESR) supports the New Zealand Police in crime scene investigation, evidence collection and forensic DNA profiling. The DNA team has optimized their analysis workflow to gain the most probative information possible, especially from sexual assault evidence kits.<\/p>\n","protected":false},"author":115,"featured_media":13138,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[71],"tags":[],"division":[],"class_list":{"0":"post-13136","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-forensics","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Forensic DNA Profiling - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/improving-dna-profiling\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Forensic DNA Profiling\" \/>\n<meta property=\"og:description\" content=\"Improving The Sexual Assault Workflow Using Applied Biosystems Forensic Solutions The Institute of Environmental Science and Research Limited \u00a0(ESR) supports the New Zealand Police in crime scene investigation, evidence collection and forensic DNA profiling. The DNA team has optimized their analysis workflow to gain the most probative information possible, especially from sexual assault evidence kits.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/improving-dna-profiling\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2020-01-28T21:09:28+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2021-03-22T17:35:11+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/01\/Screen-Shot-2020-01-28-at-1.08.26-PM-1024x533.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1024\" \/>\n\t<meta property=\"og:image:height\" content=\"533\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Angie Lackey\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Angie Lackey\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/improving-dna-profiling\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/improving-dna-profiling\\\/\"},\"author\":{\"name\":\"Angie Lackey\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/aee4ecb3303ff14eb0ffd6f4b0554fab\"},\"headline\":\"Forensic DNA Profiling\",\"datePublished\":\"2020-01-28T21:09:28+00:00\",\"dateModified\":\"2021-03-22T17:35:11+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/improving-dna-profiling\\\/\"},\"wordCount\":200,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/improving-dna-profiling\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2020\\\/01\\\/Screen-Shot-2020-01-28-at-1.08.26-PM.png\",\"articleSection\":[\"Forensics\"],\"inLanguage\":\"en-US\"},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/improving-dna-profiling\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/improving-dna-profiling\\\/\",\"name\":\"Forensic DNA Profiling - 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Can You Perform SNP Genotyping Without DNA Purification? The short answer: In many research workflows, direct-from-lysate SNP genotyping can be performed without traditional DNA purification\u2014when appropriate chemistries and verification procedures are used. 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