{"id":13548,"date":"2020-05-18T20:51:31","date_gmt":"2020-05-18T20:51:31","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=13548"},"modified":"2020-05-18T20:56:36","modified_gmt":"2020-05-18T20:56:36","slug":"high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/","title":{"rendered":"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging"},"content":{"rendered":"

Cameron Smurthwaite MSc, MBA, explores new innovations in high-content screening and how Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.<\/em><\/p>\n

<\/a>The theme of the Society for Laboratory Automation and Screening\u2019s SLAS2020 conference, held in San Diego, California, was \u2018Level Up Your Science.\u2019 Given the increased participation of small biotech and pharmaceutical companies this year, it\u2019s obvious that the field has indeed leveled up. From equipment and hardware to software and process improvements, the conference was filled with automation solutions designed to streamline, enable scale, and optimize workflows for deeper, more insightful analyses.<\/p>\n

As pharmaceutical and biotech companies clearly have a dynamic and symbiotic relationship, we wanted to understand how the breadth of the Thermo Fisher Scientific portfolio enables both ends of the biotech-pharma spectrum. Visikol, a biotechnology Contract Research Organization (CRO) established in 2014 – who utilize High-Content Screening (HCS) within their workflows, spoke with us about their services and how they use some of our premier brands and products to advance the drug discovery process.<\/p>\n

See what new innovations for the CeLLInsight\u00ae CX7 LED and LZR High-Content Screening platform are coming next!<\/a><\/p>\n

Can you introduce yourself, your institution, and the services provided?<\/h2>\n

I\u2019m Erin Edwards, head of services at Visikol. As a CRO we specialize in a number of different assays that include imaging as one of the primary endpoints. These include running in vitro assays, cell-based assays, 2D and 3D cell culture models, where we will work with clients to evaluate their compounds on those models. We also take in projects from clients who have already generated 3D cell culture models, or other tissues, and label them in 3D, pull out any sort of quantitative insights by complex analysis of those 3D tissues and cell culture models and deliver a report to the client in order to drive forward their drug discovery programs.<\/p>\n

How does the Thermo Scientific CeLLInsight\u00ae CX7 LZR High-Content Screening Platform help you image large 3D models or thick tissues?<\/h2>\n

The Thermo Scientific CeLLInsight\u00ae CX7 LZR High-Content Screening Platform<\/a> is a great system because it allows us to essentially perform automated confocal microscopy on a number of samples in a much shorter amount of time than if you were to using standard upright confocal microscopy. Confocal imaging mode on the CeLLInsight\u00ae CX7 LZR is super important when you\u2019re trying to visualize 3D cell culture models or 3D tissues, because it really allows you to get deeper into those tissues and make sure you\u2019re actually analyzing the entire tissue of sizes up to 1,000 uM. To do so we also use clearing reagents, in particular the CytoVista\u2122 reagents. These allow us to clear and image the entire 3D cell culture models or more complex tissues. Also, the utilization of CytoVista\u2122 clearing reagents eliminates\u00a0the need for water immersion objectives, which are difficult to maintain during screening campaigns. So we really leverage those technologies, the combination of CytoVista\u2122 clearing reagents with the High-Content Screening Platform in order to extract the most quantitative insights and fully characterize these models.<\/p>\n

Can you tell us more about how CytoVista\u2122\u00a0helps to analyze 3D cell culture models?<\/h2>\n

Another unique thing about the CytoVista\u2122 reagent is the ability to multiplex immunofluorescence and still get good clearing results. Many of the other clearing approaches out there are not amenable to immunofluorescence labeling. Not only can you use some small molecule dyes, but you can also add antibody labels on there, and do a full characterization of your 3D cell culture models. This becomes really important, when you have regionalized activity within a larger 3D cell culture model. If you think about having a necrotic core, or have the cells on the outer most layer exposed to a therapeutic; these regional differences really become important to understanding what\u2019s really going on when you treat a 3D cell culture model with a compound of interest.<\/p>\n

What are some of the features and benefits related to scaling for high-throughput screening assay?<\/h2>\n

The advantage of using the Thermo Scientific CeLLInsight\u00ae CX7 LZR Platform is that it reduces the time in the imaging phase.<\/p><\/blockquote>\n

If you have to go through each individual tissue and image it in confocal microscopy mode, it\u2019ll take hours or even days and require constant interface with the equipment. However, with the Thermo Scientific CeLLInsight\u00ae CX7 LZR we are able to accomplish that same amount of imaging in a mere one two maybe three hours, depending on how many different types of endpoints you\u2019re looking at. This especially becomes important when you\u2019re talking about doing a high-content screen, something where you\u2019re trying to get a lot more out of the 3D cell culture models. You might have 4 or 5 different colors, different channels that you\u2019re trying to image in, maybe 15 or 20 Z-steps, in a 96 or 384 well plate. This would normally take days to go through to scan each individual tissue, so the equipment really allows us to do that in a much more high-throughput manner.<\/p>\n

What kinds of research applications are common for you to work on?<\/h2>\n

We don\u2019t have a particular therapeutic area that we narrow into, instead we work in a variety of areas, so it allows us to get exposure to different therapeutic contexts. Things like immuno-oncology where we will look at immune cell invasion into tumor spheroids. Again, clearing and high-content confocal microscopy are extremely important for this assay. It allows us to see how deeply immune cells are actually infiltrating into spheroids, and how a client\u2019s compound might affect that ability for those cells to infiltrate. That\u2019s just one example in the immuno-oncology space, but we also do a number of assays in other areas, such as toxicology. We look at liver toxicology, cardio-tox, neuro-tox and try to get a mechanistic understanding of a compound. We also do some disease modeling, for instance in NASH (Non-Alcoholic Steatohepatitis) – so there\u2019s a lot of really interesting projects, and 3D approaches can really bring a lot more to the table in terms of recapitulating a type of toxicity or a disease state in order to fully evaluate the effects of a compound.<\/p>\n

What does success look like for Visikol?<\/h2>\n

I\u2019m really excited to see where the future of these kind of high-content imaging projects can take us.<\/p>\n

We are always looking to drive forward the drug discovery process at an accelerated pace, and that\u2019s something that the Thermo Scientific CeLLInsight\u00ae CX7 LZR allows us to do.<\/p><\/blockquote>\n

By being able to image tissues and 3D cell culture models more quickly, using a very relevant cell culture model, allows us to provide accurate and efficient turnaround times for our clients, in order to drive forward the drug discovery process and hopefully improve patient outcomes.<\/p>\n

<\/a>What\u2019s next from Thermo Fisher Scientific?<\/h2>\n

At Thermo Fisher Scientific, we have a long history of partnering with our high-content screening customers and delivering compelling solutions that help them advance and accelerate their science. Our HCS Studio software, already well known in the high-content community for its powerful cell analysis capabilities, will soon be further enhanced with the release of HCS Studio 4.0, featuring EurekaScan Finder.<\/p>\n

EurekaScan is a new \u201cseek and find\u201d feature for the CeLLInsight\u00ae CX7 LED and LZR HCS platforms aimed at accelerating discovery by automating the identification and capture of irregularly seeded biological samples, including spheroids and organoids,\u00a0at progressively higher magnifications. With EurekaScan applied, specimens including spheroids and tissues are identified during \u201cseek\u201d operations at low magnification and once \u201cfound,\u201d efficiently scanned at higher magnifications for optimal resolution. EurekaScan is allows the user to first identify samples using low magnification across large surface areas, capture them at intermediate magnification, then evaluate them for rare events or improved resolution at higher magnifications. With EurekaScan, efficiencies in total scan times and corresponding file memory consumption are accomplished. Moreover, object identification and validation parameters are applied in real time during EurekaScan operation, so scientists have confidence that samples are being adaptively examined.<\/p>\n

Learn more about CytoVista\u2122 clearing reagents<\/a> and Invitrogen\u2122\u00a0mounting media and antifades<\/a>
\nLearn more about High-Content Screening basics<\/a>
\nLearn more about High-Content Screening applications<\/a>
\nLearn more about the CeLLInsight\u00ae CX7 LZR HCS platform<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

Cameron Smurthwaite MSc, MBA, explores new innovations in high-content screening and how Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process. The theme of the Society for Laboratory Automation and Screening\u2019s SLAS2020 conference, held in San Diego, California, was \u2018Level Up Your Science.\u2019 Given the increased participation of small<\/p>\n","protected":false},"author":120,"featured_media":13718,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[113,172],"tags":[],"division":[],"class_list":{"0":"post-13548","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-events-and-tradeshows","8":"category-general","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nHigh Content Screening Platform Innovations - Thick tissue, 3D model imaging<\/title>\n<meta name=\"description\" content=\"New innovations in High Content Screening. How Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging\" \/>\n<meta property=\"og:description\" content=\"New innovations in High Content Screening. How Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2020-05-18T20:51:31+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-18T20:56:36+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1286\" \/>\n\t<meta property=\"og:image:height\" content=\"726\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Behind The Bench Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Behind The Bench Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"7 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/\"},\"author\":{\"name\":\"Behind The Bench Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"headline\":\"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging\",\"datePublished\":\"2020-05-18T20:51:31+00:00\",\"dateModified\":\"2020-05-18T20:56:36+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/\"},\"wordCount\":1380,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2020\\\/05\\\/Featured-Image.jpg\",\"articleSection\":[\"Events and Tradeshows\",\"General\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/\",\"name\":\"High Content Screening Platform Innovations - Thick tissue, 3D model imaging\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2020\\\/05\\\/Featured-Image.jpg\",\"datePublished\":\"2020-05-18T20:51:31+00:00\",\"dateModified\":\"2020-05-18T20:56:36+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"description\":\"New innovations in High Content Screening. How Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2020\\\/05\\\/Featured-Image.jpg\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2020\\\/05\\\/Featured-Image.jpg\",\"width\":1286,\"height\":726},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\",\"name\":\"Behind the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\",\"name\":\"Behind The Bench Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"caption\":\"Behind The Bench Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/behind-the-bench-staff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"High Content Screening Platform Innovations - Thick tissue, 3D model imaging","description":"New innovations in High Content Screening. How Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/","og_locale":"en_US","og_type":"article","og_title":"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging","og_description":"New innovations in High Content Screening. How Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.","og_url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/","og_site_name":"Behind the Bench","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2020-05-18T20:51:31+00:00","article_modified_time":"2020-05-18T20:56:36+00:00","og_image":[{"width":1286,"height":726,"url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg","type":"image\/jpeg"}],"author":"Behind The Bench Staff","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Behind The Bench Staff","Est. reading time":"7 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/"},"author":{"name":"Behind The Bench Staff","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"headline":"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging","datePublished":"2020-05-18T20:51:31+00:00","dateModified":"2020-05-18T20:56:36+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/"},"wordCount":1380,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg","articleSection":["Events and Tradeshows","General"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/","url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/","name":"High Content Screening Platform Innovations - Thick tissue, 3D model imaging","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg","datePublished":"2020-05-18T20:51:31+00:00","dateModified":"2020-05-18T20:56:36+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"description":"New innovations in High Content Screening. How Visikol, a biotechnology CRO, utilizes HCS within their workflows to advance the drug discovery process.","breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg","contentUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg","width":1286,"height":726},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/high-content-screening-platform-innovations-enabling-thick-tissue-and-3d-model-imaging\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/behindthebench\/"},{"@type":"ListItem","position":2,"name":"High-Content Screening Platform Innovations: Enabling thick tissue and 3D model imaging"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/behindthebench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c","name":"Behind The Bench Staff","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","caption":"Behind The Bench Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"}]}},"taxonomy_info":{"category":[{"value":113,"label":"Events and Tradeshows"},{"value":172,"label":"General"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image-1024x578.jpg",760,429,true],"author_info":{"display_name":"Behind The Bench Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"},"comment_info":0,"category_info":[{"term_id":113,"name":"Events and Tradeshows","slug":"events-and-tradeshows","term_group":0,"term_taxonomy_id":113,"taxonomy":"category","description":"","parent":0,"count":89,"filter":"raw","meta":[],"cat_ID":113,"category_count":89,"category_description":"","cat_name":"Events and Tradeshows","category_nicename":"events-and-tradeshows","category_parent":0},{"term_id":172,"name":"General","slug":"general","term_group":0,"term_taxonomy_id":172,"taxonomy":"category","description":"","parent":0,"count":157,"filter":"raw","meta":[],"cat_ID":172,"category_count":157,"category_description":"","cat_name":"General","category_nicename":"general","category_parent":0}],"tag_info":false,"jetpack-related-posts":[{"id":19223,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/innovations-in-qpcr-technology\/","url_meta":{"origin":13548,"position":0},"title":"Innovations in qPCR Technology: What Researchers Need to Know","author":"Ashleigh Barlow","date":"October 29, 2025","format":false,"excerpt":"https:\/\/youtu.be\/z9Kb1xAnNcg?si=TVUIc8rUxZUOhCqA Real-time PCR, also known as quantitative polymerase chain reaction (qPCR) technology, has been a cornerstone of molecular biology research for decades. With continuous advancements, qPCR remains a critical tool for researchers worldwide. Damien Luk, Senior Director of Product Management at Thermo Fisher Scientific, shared his insights on the future\u2026","rel":"","context":"In "Developmental Biology"","block_context":{"text":"Developmental Biology","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/developmental-biology\/"},"img":{"alt_text":"A lineup of Thermo Fisher Scientific QuantStudio real-time PCR systems, including QuantStudio 3, 5, 6 Pro, 7 Pro, and 12K Flex instruments, displayed against a blue gradient background.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/qPCR-Blog-Cover-Image.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19435,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/scaling-human-relevant-oncology-testing-oncopro-tumoroids-on-screenin3ds-upscale-platform\/","url_meta":{"origin":13548,"position":1},"title":"Scaling Human-Relevant Oncology Testing: OncoPro Tumoroids on ScreenIn3D\u2019s UpScale Platform","author":"Behind The Bench Staff","date":"February 25, 2026","format":false,"excerpt":"The United States National Institutes of Health has prioritized human cell-based in vitro approaches to \u201cmodel human disease and capture human variability and patient-specific characteristics,\u201d complementing the U.S. Food and Drug Administration\u2019s goals to use advanced in vitro assays as a New Approach Methodology (NAM) that could reduce animal testing.\u2026","rel":"","context":"In "Cancer Research"","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19193,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/advancing-cancer-cell-based-assays-with-assay-ready-tumoroids\/","url_meta":{"origin":13548,"position":2},"title":"Advancing Cancer Cell-Based Assays with Assay-Ready Tumoroids","author":"Behind The Bench Staff","date":"October 28, 2025","format":false,"excerpt":"Cancer research is continually evolving, and patient-derived tumoroids are playing a crucial role in this progress. These three-dimensional (3D) cell culture models, also known as cancer organoids, closely mimic the genetic profile, gene expression patterns, and architecture of actual tumors. Therefore, tumoroids are increasingly being used for basic research, drug\u2026","rel":"","context":"In "Cancer Research"","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Microscopic image of HuLu051921 tumoroids stained with Hematoxylin and Eosin at 20x magnification, field 1, bottom section of the slide","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19860,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/choose-sybr-green-qpcr-master-mix\/","url_meta":{"origin":13548,"position":3},"title":"Choosing the Right SYBR Green Master Mix for Your Research and Budget","author":"Behind The Bench Staff","date":"July 28, 2026","format":false,"excerpt":"Every qPCR experiment begins with a series of decisions. How many targets should you analyze? How many samples and replicates are enough? Should you expand your sample cohort or reserve budget for downstream analytical validation studies? The answers depend not only on the scientific question, but also on the resources\u2026","rel":"","context":"In "Lab Tips and Tricks"","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"Research scientist preparing samples for a real-time PCR experiment in a molecular biology laboratory.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Blog-1280-x-720_Michealla-Scott.jpeg?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19966,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-assay-optimization-qpcr\/","url_meta":{"origin":13548,"position":4},"title":"Building Better Multiplex Panels: Identifying Assay Interactions Early","author":"Ashleigh Barlow","date":"September 8, 2026","format":false,"excerpt":"Multiplex qPCR has transformed molecular research by enabling scientists to detect multiple targets in a single reaction. The benefits are clear: reduced sample consumption, lower costs, faster workflows, and more data from every experiment. However, designing successful multiplex assays isn't always straightforward. Traditionally, identifying problematic assay combinations required extensive wet-lab\u2026","rel":"","context":"In "Lab Tips and Tricks"","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"For Research Use Only. Not for use in diagnostic procedures. \u00a9 2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19257,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/cryopreservation-advancements-in-human-stem-cell-derived-neurons\/","url_meta":{"origin":13548,"position":5},"title":"Cryopreservation: Advancements in Human Stem Cell-Derived Neurons","author":"Behind The Bench Staff","date":"December 3, 2025","format":false,"excerpt":"The field of cryopreservation has seen advancements, particularly with the ability to preserve human stem cell-derived neurons. This progress holds great promise for research and therapeutic applications, helping to ensure the availability of high-quality neuronal cultures. What is Cryopreservation? Cryopreservation involves cooling cells to sub-zero temperatures to halt biological activity\u2026","rel":"","context":"In "General"","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"Illustration of active nerve cell synapses showing neural connections, related to cryopreservation research.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/active-nerve-cell-synapses.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/active-nerve-cell-synapses.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/active-nerve-cell-synapses.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/active-nerve-cell-synapses.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/active-nerve-cell-synapses.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/active-nerve-cell-synapses.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2020\/05\/Featured-Image.jpg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/13548","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=13548"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/13548\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media\/13718"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=13548"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=13548"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=13548"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=13548"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}