{"id":16775,"date":"2022-06-27T11:52:28","date_gmt":"2022-06-27T11:52:28","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/behindthebench\/?p=16775"},"modified":"2022-07-08T19:50:52","modified_gmt":"2022-07-08T19:50:52","slug":"two-applications-one-instrument-assay","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/two-applications-one-instrument-assay\/","title":{"rendered":"Two Applications One Instrument"},"content":{"rendered":"<h1><strong>What are the Benefits of Performing Proteomics and Genomics Assays on the Same Instrument?<\/strong><\/h1>\n<p>&nbsp;<\/p>\n<h2><strong>Protein and Gene Expression Immunoassays<\/strong><\/h2>\n<p>As cell analysis techniques evolve, biological systems are investigated in greater depths, and many recent advances have been focused on genomics. Genetic sequencing provides comprehensive information on mRNA expression and the genome. However, it does not provide the full biological picture due to its stochastic nature. Proteins are more stable, present in higher concentrations, and play a direct role in maintaining cellular functions. To accurately define cellular states, understanding both proteomics and genomics provides a more holistic picture than either one in isolation. Traditional gene expression assays and immunoassays have limitations such as:<\/p>\n<ul>\n<li>High sample consumption<\/li>\n<li>Time consuming setup<\/li>\n<li>Variability between users<\/li>\n<li>Complex data analysis<\/li>\n<\/ul>\n<p>Hence, the ability to analyze gene and protein expression on the same platform offers a unique opportunity to interrogate two \u201c-omics\u201d aspects with greater ease.<\/p>\n<h2><strong>One Platform for Multi-omics<\/strong><\/h2>\n<p>Several approaches are being developed for analyzing mRNA and protein expression. Many are based on microscopy-based technologies. Microarrays and RNA sequencing technologies can be used for genome expression whereas, mass spectrometry has been used for proteomic analysis in human samples. However, some of these techniques can only analyze targeted mRNA and surface proteins. Other methods can measure intracellular proteins, but they are limited to only a handful of proteins and require cell fixation and large volumes of sample.<\/p>\n<h2><strong>Unique Solution<\/strong><\/h2>\n<p>One solution overcomes most of these challenges and offers measurement of intra- or extracellular proteins and mRNA expression utilizing small sample volumes \u2013 <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex\/procartaplex-immunoassays.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay1\">Invitrogen\u2122 ProcartaPlex\u2122 immunoassays<\/a> and <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/gene-expression-analysis-genotyping\/quantigene-rna-assays\/quantigene-plex-assay.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay2\">QuantiGene\u2122 Plex gene expression assays<\/a> using xMap<sup>\u00ae<\/sup> Technology on the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex\/luminex-instruments.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay3\">Luminex\u00ae instrument platform.<\/a> This flexibility of the Luminex platform offers greater efficiency and lowers start up spend. It eliminates the need for a separate instrument for both protein and genetic analysis. Also, multiplexing assays using xMap<sup>\u00ae<\/sup> technology enables scientists to measure multiple biomarkers from a single sample, which can generate more data with less sample in a short period of time, resulting in cost savings per biomarker analyzed.<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex\/procartaplex-immunoassays.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay4\">The ProcartaPlex immunoassays<\/a> cover more than 600 cytokines, chemokines, growth factors, and other protein targets from human, mouse, rat, non-human primate, canine, and other species. <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/gene-expression-analysis-genotyping\/quantigene-rna-assays\/quantigene-plex-assay.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay5\">The QuantiGene Plex assays<\/a> offer a fast and high-throughput solution for multiplex gene expression analysis of thousands of different mRNA targets, with the ability to measure up to 80 genes of interest simultaneously. This significantly increases the amount of datapoints measured with traditional gene expression technologies like qRT-PCR. Data provided by ProcartaPlex and QuantiGene assays are complementry and the assays can be run on any Luminex platform, facilitating holistic understanding of biological systems, all from one instrument.<\/p>\n<h2><strong>Practical Applications<\/strong><\/h2>\n<p>Inflammation and Immune response are complex processes. Moreover, individuals may show different levels of immune response to viral and other types of infections. Hence, deeper knowledge of mRNA expression and cytokine release patterns is necessary to understand clinical symptoms and personalize treatment. Infection and dysfunctional immune reactions are both controlled by a combination of mRNA expression and immune factors such as cytokines\/chemokines. Hence, studying both these factors are necessary for understanding the disease and immune response.<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex\/luminex-instruments.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay6\">The Luminex platform<\/a> for gene expression and protein secretion analysis is very practical as illustrated by real-life examples. The QuantiGene Plex hybridization-based assays for RNA quantitation and the ProcartaPlex immunoassays for protein quantitation have been successfully used for cytokine storm analysis and SARS-CoV-2 research. These assays have been used in bacterial disease research as well viral research. A research study on Meningitic <em>Escherichia coli<\/em> used QuantiGene Plex 2.0 Assay for cytokine mRNA quantification and ProcartaPlex Multiplex Immunoassays for measuring cytokines of brain lysates.<\/p>\n<h2><strong>Luminex Offers One Solution<\/strong><\/h2>\n<p>Combining <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/gene-expression-analysis-genotyping\/quantigene-rna-assays\/quantigene-plex-assay.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay7\">QuantiGene assays<\/a> with <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex\/procartaplex-immunoassays.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay8\">ProcartaPlex immunoassays<\/a> on <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex\/luminex-instruments.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay9\">Luminex instruments<\/a> offers a comprehensive approach to understanding biological systems. It provides reliability, flexibility, and efficiency to your genomic and proteomic research.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-16776 size-large\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/06\/PG2631-PJT9046-COL020405-PCA-Immunology-Campg-Image-PPX_QG-Blog-Global-1600x350-F-1024x224.jpg\" alt=\"Luminex for protein and gene expression assay\" width=\"760\" height=\"166\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/06\/PG2631-PJT9046-COL020405-PCA-Immunology-Campg-Image-PPX_QG-Blog-Global-1600x350-F-1024x224.jpg 1024w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/06\/PG2631-PJT9046-COL020405-PCA-Immunology-Campg-Image-PPX_QG-Blog-Global-1600x350-F-300x66.jpg 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/06\/PG2631-PJT9046-COL020405-PCA-Immunology-Campg-Image-PPX_QG-Blog-Global-1600x350-F-768x168.jpg 768w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/06\/PG2631-PJT9046-COL020405-PCA-Immunology-Campg-Image-PPX_QG-Blog-Global-1600x350-F-1536x336.jpg 1536w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2022\/06\/PG2631-PJT9046-COL020405-PCA-Immunology-Campg-Image-PPX_QG-Blog-Global-1600x350-F.jpg 1600w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/><\/p>\n<h2><strong>Learn more about the product in this blog:<\/strong><\/h2>\n<h3><\/h3>\n<h3><strong>Resources and Solutions for ProcartaPlex<\/strong><strong> immunoassay and QuantiGene assay<\/strong><\/h3>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/antibodies\/immunoassays\/procartaplex-assays-luminex.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay10\">Luminex platform<\/a> \u2013 Quantitative protein and RNA assays with superior throughput<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/luminex\/?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay11\">ProcartaPlex Panel Configurator<\/a> \u2013 This online tool helps you design an effective protein panel for your specific application<\/li>\n<li><a href=\"https:\/\/assets.fishersci.com\/TFS-Assets\/LSG\/brochures\/procartaplex-multiplex-immunoassays-brochure.pdf?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay12\">ProcartaPlex Handbook<\/a> \u2013 What would the ability to simultaneously quantitate as many as 80 cytokines or other soluble biomarkers from a single 25 \u03bcL sample do for your research?<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/technical-resources\/technical-reference-library\/antibodies-immunoassays-support-center\/luminex-assays-support.html?icid=bid_pca_aup_ro1_co_1434_pjt9046_COL020405_0s0_blg_op_awa_kt_s00_assay13\">ProcartaPlex Assays Support<\/a> \u2013 Explore the \u201cGetting Started\u201d and \u201cTroubleshooting\u201d sections for solutions to top inquiries and common problems.<\/li>\n<\/ul>\n<h3><strong>References:<\/strong><\/h3>\n<ul>\n<li>Identification of Immune Activation Markers in the Early Onset of COVID-19 Infection, Johannes J. Kovarik et.al., Cell. Infect. Microbiol., 03 September 2021<\/li>\n<li>Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood\u2013Brain Barrier Disruption via Inhibiting Proteinase 3\/Protease-Activated Receptor 2 Axis, Bojie Xu et.al., frontiers in Cellular Neuroscience, Feb 2022<\/li>\n<li>Cross-tissue Analysis of Gene and Protein Expression in Normal and Cancer Tissues, Idit Kosti et.al.,Scientific Reports volume 6, 2016<\/li>\n<li>MiRNA Profiling in Plasma and Placenta of SARS-CoV-2-Infected Pregnant Women, Irma Saulle et.al., Cells 2021<\/li>\n<\/ul>\n<h3><strong>Additional blogs:<\/strong><\/h3>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/immunoassay-more-results-from-a-single-sample\/\">How Much Data Can You Get from a Single Sample?<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/flow-cytometry-panel-design\/\">How Can You Bring Panel Design Up to Speed?<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/what-are-ways-of-expanding-flow-cytometry-panels\/\">What are Ways of Expanding Flow Cytometry Panels?<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-cell-sorting-is-becoming-an-even-more-powerful-tool\/\">How Cell Sorting is Becoming an Even More Powerful Tool?<\/a><\/li>\n<\/ul>\n<p><em>Luminex, xMAP, MAGPIX are registered trademarks of Luminex Corporation.<\/em><\/p>\n<p><em>For Research Use Only. Not for use in diagnostic procedures.<\/em><\/p>\n","protected":false},"excerpt":{"rendered":"<p>What are the Benefits of Performing Proteomics and Genomics Assays on the Same Instrument? &nbsp; Protein and Gene Expression Immunoassays As cell analysis techniques evolve, biological systems are investigated in greater depths, and many recent advances have been focused on genomics. Genetic sequencing provides comprehensive information on mRNA expression and the genome. However, it does<\/p>\n","protected":false},"author":120,"featured_media":16776,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[135],"tags":[2281,159],"division":[],"class_list":{"0":"post-16775","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-immunology","8":"tag-immunoassays","9":"tag-immunology","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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