{"id":18703,"date":"2025-02-21T08:00:13","date_gmt":"2025-02-21T08:00:13","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=18703"},"modified":"2025-03-04T18:50:50","modified_gmt":"2025-03-04T18:50:50","slug":"discover-the-future-of-dna-sample-prep-with-me-kayli-carrillo","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/discover-the-future-of-dna-sample-prep-with-me-kayli-carrillo\/","title":{"rendered":"Discover the future of DNA sample prep with me, Kayli Carrillo"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"alignright\" style=\"width: auto;height: auto\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/Kaylie-Carillo-photo73-150x150.jpg\" alt=\"\" width=\"150\" height=\"150\" align=\"left\" \/><\/p>\n<p>Hey everyone! I\u2019m Kayli Carrillo, a PhD student in the Forensic Science Department at Sam Houston State University (SHSU). I&#8217;m super excited to share my experience with the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/industrial\/forensics\/human-identification\/forensic-dna-analysis\/sample-preparation-extraction\/hid-nimbus-presto-system.html\">Applied Biosystems\u2122 HID NIMBUS\u00ae Presto QNA System<\/a>. SHSU was fortunate enough to have early access to the HID NIMBUS Presto QNA System, and I was tasked with putting the system to the test. So, I got to unbox and challenge the instrument with interesting forensic-type samples.<\/p>\n<p><strong>What is the HID NIMBUS Presto QNA System?<\/strong><\/p>\n<p>If your goal is accurate and efficient DNA analysis, you\u2019re going to be pleased to learn that the system is an all-in-one, walk-away automated sample prep instrument. This cutting-edge device is designed to make the process of gathering and analyzing data smoother and more efficient. It performs DNA sample extraction, quantification plate setup, normalization, and amplification plate setup. You\u2019re going to love it, whether you\u2019re a tech lead or an analyst.<\/p>\n<p><strong>Proof! <\/strong><\/p>\n<p>Prior to the product\u2019s release I was able to put the system through its paces and test challenging samples, including buried, burned, and surface decomposed bones. The HID NIMBUS Presto QNA System performed as well as, and in many cases better than, manual extraction.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-18704 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HID-NIMBUS-Presto-QNA-vs-Manual-Extraction.jpg\" alt=\"\" width=\"720\" height=\"405\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HID-NIMBUS-Presto-QNA-vs-Manual-Extraction.jpg 720w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HID-NIMBUS-Presto-QNA-vs-Manual-Extraction-300x169.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/p>\n<p>&nbsp;<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-18705 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HID-NIMBUS-Presto.jpg\" alt=\"\" width=\"720\" height=\"405\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HID-NIMBUS-Presto.jpg 720w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HID-NIMBUS-Presto-300x169.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/p>\n<p>Additional samples like hair, nails, and teeth from cadavers were also tested; as with the bone samples, the results from these samples were comparable to or better than those processed in a manual workflow.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-18706 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HIDNP-QNA-vs-Manual.jpg\" alt=\"\" width=\"720\" height=\"405\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HIDNP-QNA-vs-Manual.jpg 720w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/02\/HIDNP-QNA-vs-Manual-300x169.jpg 300w\" sizes=\"auto, (max-width: 720px) 100vw, 720px\" \/><\/p>\n<p><strong>Key findings\u00a0 <\/strong><\/p>\n<ul>\n<li><strong>Accuracy and precision<\/strong>\u2014The HID NIMBUS Presto QNA System delivers accurate and precise results.<\/li>\n<li><strong>Ease of use<\/strong>\u2014The system is super user-friendly, so even if you\u2019re not a techie, you\u2019ll be able to easily navigate.<\/li>\n<li><strong>Versatility<\/strong>\u2014Whether you\u2019re processing 96 or 24 samples, the HID NIMBUS Presto QNA System scales to your needs.<\/li>\n<\/ul>\n<p>If you would like to see more proof, watch this <a href=\"https:\/\/www.forensicmag.com\/615024-Improving-Casework-Workflow-Efficiency-without-Compromising-Quality-Automated-Methods-and-Data-driven-Solutions\/\">webinar<\/a> in which I detail the test and report on the results.<\/p>\n<p><strong>Unboxing<\/strong><\/p>\n<p>Since we live in the digital era, I thought it would be fun to create a series of unboxing videos about the HID NIMBUS Presto QNA System. Watch my videos as I take you through what to expect from the system. I\u2019ll share a range of features and tips, from basic to advanced. I promise it\u2019s going to be both informative and fun!<\/p>\n<ul>\n<li><a href=\"https:\/\/www.linkedin.com\/posts\/kayli-carrillo-929a94193_forensicscience-womeninstem-forensicbiology-activity-7288236657282060290-Zvnk?utm_source=share&amp;utm_medium=member_desktop\">Video 1 &#8211; Introduction<\/a><\/li>\n<li><a href=\"https:\/\/www.linkedin.com\/posts\/kayli-carrillo-929a94193_forensicscience-womeninstem-graduateschool-activity-7290775135316316160-5yxd?utm_source=share&amp;utm_medium=member_desktop\">Video 2 &#8211; Instrument Tour<\/a><\/li>\n<li><a href=\"https:\/\/www.linkedin.com\/posts\/kayli-carrillo-929a94193_forensicscience-womeninstem-activity-7293339511562911744-bFxW?utm_source=share&amp;utm_medium=member_desktop\">Video 3 &#8211; Purification<\/a><\/li>\n<li><a href=\"https:\/\/www.linkedin.com\/posts\/kayli-carrillo-929a94193_forensicbiology-thermofisherscientific-womeninstem-activity-7295881492969177090--cJP?utm_source=share&amp;utm_medium=member_desktop&amp;rcm=ACoAAAA2VeIBSo3gxQtKUOycXIltZvxSgqZNuuc\">Video 4 &#8211; Quantification and Amplification Setup<\/a><\/li>\n<li><a href=\"https:\/\/www.linkedin.com\/posts\/kayli-carrillo-929a94193_thats-a-wrap-our-final-video-in-the-activity-7300951999276097537-0sKO?utm_source=share&amp;utm_medium=member_desktop&amp;rcm=ACoAAAA2VeIBSo3gxQtKUOycXIltZvxSgqZNuuc\">Video 5 &#8211; Wrap Up<\/a><\/li>\n<\/ul>\n<p>I\u2019m so excited to share this journey with you! Connect with me on LinkedIn to learn more about me and my experience with the HID NIMBUS Presto QNA System. Feel free to leave comments\u2014I\u2019d love to hear your thoughts and answer any questions.<\/p>\n<p><strong>Resources <\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/life-science\/sample-prep-efficiency-webinar.html\">Webinar: Improving sample prep efficiency and data quality of challenging DNA samples<\/a><\/li>\n<li><a href=\"https:\/\/www.forensicmag.com\/615024-Improving-Casework-Workflow-Efficiency-without-Compromising-Quality-Automated-Methods-and-Data-driven-Solutions\/\">Webinar: Improving casework workflow efficiency without compromising quality: automated methods and data-driven solutions<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/TFS-Assets\/GSD\/brochures\/App-Note-HIDNP-QNA-Enhancing-forensic-efficiency-automated-casework.pdf\">Application note: Enhancing forensic efficiency: automated casework workflows with the HID NIMBUS Presto QNA System<\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/TFS-Assets\/GSD\/Application-Notes\/App-Note-improve-purification-HID-samples.pdf\">Application note: Application note: Improve purification turnaround times for challenging forensic samples<\/a><\/li>\n<\/ul>\n<p><strong>For Research, Forensic or Paternity Use Only. Not for use in diagnostic procedures.<\/strong><br \/>\n\u00a9 2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. NIMBUS is a registered trademark of Hamilton Co.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Hey everyone! I\u2019m Kayli Carrillo, a PhD student in the Forensic Science Department at Sam Houston State University (SHSU). I&#8217;m super excited to share my experience with the Applied Biosystems\u2122 HID NIMBUS\u00ae Presto QNA System. SHSU was fortunate enough to have early access to the HID NIMBUS Presto QNA System, and I was tasked with<\/p>\n","protected":false},"author":1563,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[71],"tags":[],"division":[],"class_list":{"0":"post-18703","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-forensics","7":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - 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Prove it.","author":"Ashleigh Barlow","date":"August 21, 2026","format":false,"excerpt":"The chemistry of your qPCR supermix can influence analytical sensitivity, specificity, precision, and overall assay performance. Even when you keep the same instrument, assays, and samples, different master mixes can perform differently. That's why we challenge you to put your supermix to the test. 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A standardized workflow combining reliable extraction, concentration and purity assessment, application-appropriate quantification, and consistent laboratory practices can help identify sample issues early, improve reproducibility,\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"Close-up shot of a dna sequence on a screen, a digital representation of genetic code, used for analysis.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/iStock-2212923489_dnasequence-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/iStock-2212923489_dnasequence-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/iStock-2212923489_dnasequence-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/iStock-2212923489_dnasequence-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/iStock-2212923489_dnasequence-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/iStock-2212923489_dnasequence-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/18703","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/1563"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=18703"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/18703\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=18703"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=18703"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=18703"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=18703"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}