{"id":19922,"date":"2026-08-21T20:35:41","date_gmt":"2026-08-21T20:35:41","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/?p=19922"},"modified":"2026-08-21T20:43:36","modified_gmt":"2026-08-21T20:43:36","slug":"qpcr-master-mix-comparison","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/qpcr-master-mix-comparison\/","title":{"rendered":"Think your supermix is good enough? Prove it."},"content":{"rendered":"\n<figure class=\"wp-block-image aligncenter size-large\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"341\" src=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-6-main-hero-copy-1024x341.jpeg\" alt=\"\" class=\"wp-image-19940\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-6-main-hero-copy-1024x341.jpeg 1024w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-6-main-hero-copy-300x100.jpeg 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-6-main-hero-copy-768x256.jpeg 768w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-6-main-hero-copy-1536x512.jpeg 1536w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-6-main-hero-copy-2048x683.jpeg 2048w\" sizes=\"auto, (max-width: 1024px) 100vw, 1024px\" \/><\/figure>\n\n\n\n<p>The chemistry of your qPCR supermix can influence analytical sensitivity, specificity, precision, and overall assay performance. Even when you keep the same instrument, assays, and samples, different master mixes can perform differently.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-that-s-why-we-challenge-you-to-put-your-supermix-to-the-test\"><strong>That&#8217;s why we challenge you to put your supermix to the test.<\/strong><\/h2>\n\n\n\n<p>Compare it side by side with an Applied Biosystems Master Mix using your existing workflow.<\/p>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\">Take the challenge \u2013 Request a sample<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-keep-your-system-rethink-your-supermix\"><strong>Keep your system. Rethink your supermix.<\/strong><\/h2>\n\n\n\n<p>Already using a Bio-Rad\u2122 CFX Opus\u2122 or other Bio-Rad\u2122 real-time PCR system? Applied Biosystems Master Mixes have demonstrated analytical performance on Bio-Rad\u2122 CFX Opus\u2122 systems, allowing you to compare your current supermix using your existing system and workflow.<\/p>\n\n\n\n<p>To support your comparison, we&#8217;ve developed dedicated evaluation guides for some of our most popular qPCR master mixes.<\/p>\n\n\n\n<p>Each evaluation includes:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Recommended starting cycling conditions for Bio-Rad\u2122 CFX Opus\u2122 systems<\/li>\n\n\n\n<li>Step-by-step reaction setup<\/li>\n\n\n\n<li>Side-by-side evaluation scorecards<\/li>\n\n\n\n<li>Access to technical support, which includes assistance reviewing data from Bio-Rad\u2122 system run files if needed<\/li>\n<\/ul>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<p><strong>Compare qPCR Master Mix Performance<\/strong><\/p>\n\n\n\n<p>Choose the Applied Biosystems Master Mix that matches your qPCR chemistry, then compare its performance with your current supermix using the same assays, samples, and instrument.<\/p>\n\n\n\n<p><strong>Compare TaqMan Fast Advanced Master Mix to Your Probe-Based Supermix<\/strong><\/p>\n\n\n\n<p>Low-copy targets can be particularly challenging in multiplex qPCR, where analytical sensitivity, precision, and PCR efficiency can all influence reliable quantification. In comparative testing on a Bio-Rad\u2122 CFX Opus\u2122 Real-Time PCR System, TaqMan Fast Advanced Master Mix was evaluated against Bio-Rad\u2122 SsoAdvanced\u2122 Universal Probes Supermix, Bio-Rad\u2122 iTaq\u2122 Universal Probes Supermix, and Bio-Rad\u2122 iQ\u2122 Multiplex Powermix. Under the study conditions, TaqMan Fast Advanced Master Mix demonstrated greater or equal precision across the evaluated multiplex targets compared with the Bio-Rad supermixes tested. At 0.0256 ng cDNA per reaction, replicate amplification profiles were evaluated across FAM, VIC, and JUN targets. Across the dilution series, TaqMan Fast Advanced Master Mix demonstrated PCR efficiencies of 101%, 102%, and 97% for FAM, VIC, and JUN, respectively.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full is-resized\"><img loading=\"lazy\" decoding=\"async\" width=\"1280\" height=\"720\" src=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-1-Probe-Mixes.png\" alt=\"\" class=\"wp-image-19933\" style=\"width:624px;height:auto\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-1-Probe-Mixes.png 1280w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-1-Probe-Mixes-300x169.png 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-1-Probe-Mixes-1024x576.png 1024w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-1-Probe-Mixes-768x432.png 768w\" sizes=\"auto, (max-width: 1280px) 100vw, 1280px\" \/><figcaption class=\"wp-element-caption\">Figure 1 <strong>Low-copy multiplex performance of TaqMan Fast Advanced Master Mix compared with three supermixes.<\/strong> Amplification plots at 0.0256 ng cDNA\/reaction and standard curves for FAM (blue), VIC (green), and JUN (orange) are shown for TaqMan Fast Advanced Master Mix, Bio-Rad SsoAdvanced Universal Probes Supermix, Bio-Rad iTaq Universal Probes Supermix, and Bio-Rad iQ Multiplex Powermix. Tables show slope, PCR efficiency, and R\u00b2 for each target.<br><br>* Results reflect the specific assays, experimental conditions, reagent lots, and product versions evaluated and may not be representative of performance in all applications or conditions.<\/figcaption><\/figure>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\">Request a sample of TaqMan Fast Advanced Master Mix<\/a><\/div>\n<\/div>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-19922_575e2f-f8 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-19922_575e2f-f8 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-19922_575e2f-f8\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\" \/><\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-compare-your-sybr-green-supermix-to-powerup-or-powertrack-sybr-green-master-mix\"><strong>Compare Your SYBR Green Supermix to PowerUp or PowerTrack SYBR Green Master Mix<\/strong><\/h2>\n\n\n\n<p>With SYBR Green chemistry, every fluorescent signal matters\u2014but not every signal is from your target. Nonspecific amplification and primer-dimers can contribute to fluorescence, making analytical specificity an important consideration when choosing a SYBR Green master mix.<\/p>\n\n\n\n<p>Compare your current SYBR Green supermix with PowerUp or PowerTrack SYBR Green Master Mix and evaluate performance using your own assays, samples, and qPCR system.<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-compare-sybr-green-assay-specificity-across-master-mixes\"><strong>Compare SYBR Green assay specificity across master mixes<\/strong><\/h2>\n\n\n\n<p>Melt curve analysis can help assess SYBR Green assay specificity. A single melt peak suggests amplification of a single product, while additional peaks can indicate nonspecific amplification or primer-dimer formation.<\/p>\n\n\n\n<p>In comparative testing on a Bio-Rad\u2122 CFX Opus\u2122 Real-Time PCR System, melt curve profiles were evaluated across three representative SYBR Green assays using PowerTrack and PowerUp SYBR Green Master Mixes and two Bio-Rad supermixes.<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full\"><img loading=\"lazy\" decoding=\"async\" width=\"1280\" height=\"720\" src=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-2-SYBR-Green-Mixes.png\" alt=\"\" class=\"wp-image-19934\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-2-SYBR-Green-Mixes.png 1280w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-2-SYBR-Green-Mixes-300x169.png 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-2-SYBR-Green-Mixes-1024x576.png 1024w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/SuperMix-Challenge-Blog-Figure-2-SYBR-Green-Mixes-768x432.png 768w\" sizes=\"auto, (max-width: 1280px) 100vw, 1280px\" \/><figcaption class=\"wp-element-caption\"><strong>Figure 2. SYBR Green melt curve profiles across three representative assays. <\/strong>Melt curve profiles are shown for PowerTrack SYBR Green Master Mix, PowerUp SYBR Green Master Mix, Bio-Rad SsoAdvanced Universal SYBR Green Supermix, and Bio-Rad iTaq Universal SYBR Green Supermix. qPCR was performed using universal human reference (UHR) cDNA at 1 ng\/reaction, with reactions run in triplicate on a Bio-Rad CFX Opus Real-Time PCR System.performed using universal human reference (UHR) cDNA at 1 ng\/reaction, with reactions run in triplicate on a Bio-Rad CFX Opus Real-Time PCR System.<br><br>* Results reflect the specific assays, experimental conditions, reagent lots, and product versions evaluated and may not be representative of performance in all applications or conditions.<\/figcaption><\/figure>\n\n\n\n<p><strong>Which Applied Biosystems SYBR Green Master Mix Should You Choose?<\/strong><\/p>\n\n\n\n<p>Both PowerTrack and PowerUp SYBR Green Master Mixes include premixed ROX dye and UDG. The key differences are the performance and workflow features that may matter for your application.<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td><\/td><td><strong>PowerTrack SYBR Green Master Mix<\/strong><\/td><td><strong>PowerUp SYBR Green Master Mix<\/strong><\/td><\/tr><tr><td><strong>Recommended for<\/strong><\/td><td>High analytical sensitivity &amp; reaction setup QC<\/td><td>Highest analytical specificity<\/td><\/tr><tr><td><strong>Tracking dye<\/strong><\/td><td>Yes<\/td><td>No<\/td><\/tr><tr><td><strong>Benchtop stability<\/strong><\/td><td>8 hours<\/td><td>72 hours<\/td><\/tr><tr><td><strong>DNA polymerase<\/strong><\/td><td>Taq with antibody-mediated hot start<\/td><td>Taq with dual lock double hot start<\/td><\/tr><tr><td><strong>ROX dye<\/strong><\/td><td>Included, premixed<\/td><td>Included, premixed<\/td><\/tr><tr><td><strong>UDG<\/strong><\/td><td>Included<\/td><td>Included<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-19922_a99741-71 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-19922_a99741-71 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-19922_a99741-71\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\" \/><\/div><\/div>\n\n\n\n<div class=\"wp-block-buttons is-content-justification-center is-layout-flex wp-container-core-buttons-is-layout-16018d1d wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button\"><a class=\"wp-block-button__link wp-element-button\" href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\">Request a sample of an Applied Biosystems SYBR Green Master Mix<\/a><\/div>\n<\/div>\n\n\n<style>.wp-block-kadence-spacer.kt-block-spacer-19922_9e50d3-46 .kt-block-spacer{height:60px;}.wp-block-kadence-spacer.kt-block-spacer-19922_9e50d3-46 .kt-divider{border-top-width:1px;height:1px;border-top-color:#eee;width:80%;border-top-style:solid;}<\/style>\n<div class=\"wp-block-kadence-spacer aligncenter kt-block-spacer-19922_9e50d3-46\"><div class=\"kt-block-spacer kt-block-spacer-halign-center\"><hr class=\"kt-divider\" \/><\/div><\/div>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-challenge-accepted\"><strong>Challenge accepted?<\/strong><\/h2>\n\n\n\n<p>The best way to evaluate a master mix isn&#8217;t by reading about it. It&#8217;s by running it.<\/p>\n\n\n\n<p>Request an evaluation sample, download the guide that matches your workflow, and compare an Applied Biosystems Master Mix with your current supermix. Then decide based on what your data tell you<strong>.<\/strong><\/p>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\"><strong>Request a Sample<\/strong><\/a>*<\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity is-style-wide\" \/>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-frequently-asked-questions\"><strong>Frequently Asked Questions<\/strong><\/h2>\n\n\n<style>.kt-accordion-id19922_63f9ce-b4 .kt-accordion-inner-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:10px;}.kt-accordion-id19922_63f9ce-b4 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;background:#ffffff;padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);}.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;background:#ffffff;color:#444444;padding-top:14px;padding-right:16px;padding-bottom:14px;padding-left:16px;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4 .kt-blocks-accordion-header:focus-visible{color:#444444;background:#ffffff;border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion--visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger, body:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id19922_63f9ce-b4 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{color:#444444;background:#ffffff;border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_63f9ce-b4:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}@media all and (max-width: 1024px){.kt-accordion-id19922_63f9ce-b4 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}}@media all and (max-width: 1024px){.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}}@media all and (max-width: 1024px){.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}}@media all and (max-width: 1024px){.kt-accordion-id19922_63f9ce-b4 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}}@media all and (max-width: 767px){.kt-accordion-id19922_63f9ce-b4 .kt-accordion-inner-wrap{display:block;}.kt-accordion-id19922_63f9ce-b4 .kt-accordion-inner-wrap .kt-accordion-pane:not(:first-child){margin-top:10px;}.kt-accordion-id19922_63f9ce-b4 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_63f9ce-b4 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}.kt-accordion-id19922_63f9ce-b4 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_63f9ce-b4 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}}<\/style>\n<div class=\"wp-block-kadence-accordion alignnone\"><div class=\"kt-accordion-wrap kt-accordion-id19922_63f9ce-b4 kt-accordion-has-4-panes kt-active-pane-0 kt-accordion-block kt-pane-header-alignment-left kt-accodion-icon-style-arrow kt-accodion-icon-side-right\" style=\"max-width:none\"><div class=\"kt-accordion-inner-wrap\" data-allow-multiple-open=\"false\" data-start-open=\"0\">\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-3 kt-pane19922_72d113-86\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong>Can switching supermixes improve my qPCR data?<\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p>Potentially, yes. A side-by-side comparison using the same instrument, assays, and samples can help determine whether a different qPCR master mix improves analytical sensitivity, precision, specificity, or overall assay performance for your application.<\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-4 kt-pane19922_7338b5-82\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong>Can I use Applied Biosystems Master Mixes on a Bio-Rad\u2122 qPCR instrument?<\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p>Yes. Applied Biosystems Master Mixes can be evaluated on Bio-Rad\u2122 CFX Opus\u2122 and other Bio-Rad\u2122 real-time PCR systems. The evaluation guides include recommended starting cycling conditions, and our technical support specialists can assist with workflow questions and Bio-Rad\u2122 run files if needed.<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<style>.kt-accordion-id19922_8a57cd-48 .kt-accordion-inner-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:10px;}.kt-accordion-id19922_8a57cd-48 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;background:#ffffff;padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);}.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;background:#ffffff;color:#444444;padding-top:14px;padding-right:16px;padding-bottom:14px;padding-left:16px;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48 .kt-blocks-accordion-header:focus-visible{color:#444444;background:#ffffff;border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion--visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle ) .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger, body:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:hover .kt-blocks-accordion-icon-trigger:before, body:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:after, body:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}.kt-accordion-id19922_8a57cd-48 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{color:#444444;background:#ffffff;border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger{background:#444444;}.kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_8a57cd-48:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) .kt-blocks-accordion-header.kt-accordion-panel-active .kt-blocks-accordion-icon-trigger:before{background:#ffffff;}@media all and (max-width: 1024px){.kt-accordion-id19922_8a57cd-48 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}}@media all and (max-width: 1024px){.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}}@media all and (max-width: 1024px){.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}}@media all and (max-width: 1024px){.kt-accordion-id19922_8a57cd-48 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}}@media all and (max-width: 767px){.kt-accordion-id19922_8a57cd-48 .kt-accordion-inner-wrap{display:block;}.kt-accordion-id19922_8a57cd-48 .kt-accordion-inner-wrap .kt-accordion-pane:not(:first-child){margin-top:10px;}.kt-accordion-id19922_8a57cd-48 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_8a57cd-48 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}.kt-accordion-id19922_8a57cd-48 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_8a57cd-48 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}}<\/style>\n<div class=\"wp-block-kadence-accordion alignnone\"><div class=\"kt-accordion-wrap kt-accordion-id19922_8a57cd-48 kt-accordion-has-3-panes kt-active-pane-0 kt-accordion-block kt-pane-header-alignment-left kt-accodion-icon-style-arrow kt-accodion-icon-side-right\" style=\"max-width:none\"><div class=\"kt-accordion-inner-wrap\" data-allow-multiple-open=\"false\" data-start-open=\"0\">\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-1 kt-pane19922_6acb99-61\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong>Should I compare Ct values or overall analytical performance<\/strong>?<\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p>Ct values are only one part of the comparison. When evaluating qPCR master mixes, consider analytical sensitivity, PCR efficiency, linearity, replicate precision, and analytical specificity rather than relying on Ct values alone. Learn more about evaluating qPCR reagent performance in our <a href=\"https:\/\/documents.thermofisher.com\/TFS-Assets\/GSD\/Application-Notes\/evaluate-qpcr-performance-app-note.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Real-time PCR Reagent Comparison Guide.<\/a><\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-2 kt-pane19922_fbd377-12\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong>Which Applied Biosystems Master Mix should I compare to my supermix?<\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p>Choose TaqMan Fast Advanced Master Mix if you use probe-based qPCR assays. Choose PowerUp SYBR Green Master Mix if you use SYBR Green chemistry and want to evaluate analytical specificity and amplification performance.<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n<style>.kt-accordion-id19922_23959d-79 .kt-accordion-inner-wrap{column-gap:var(--global-kb-gap-md, 2rem);row-gap:10px;}.kt-accordion-id19922_23959d-79 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;background:#ffffff;padding-top:var(--global-kb-spacing-sm, 1.5rem);padding-right:var(--global-kb-spacing-sm, 1.5rem);padding-bottom:var(--global-kb-spacing-sm, 1.5rem);padding-left:var(--global-kb-spacing-sm, 1.5rem);}.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;border-top-left-radius:0px;border-top-right-radius:0px;border-bottom-right-radius:0px;border-bottom-left-radius:0px;background:#ffffff;color:#444444;padding-top:14px;padding-right:16px;padding-bottom:14px;padding-left:16px;}.kt-accordion-id19922_23959d-79:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:after, .kt-accordion-id19922_23959d-79:not( .kt-accodion-icon-style-basiccircle ):not( .kt-accodion-icon-style-xclosecircle ):not( .kt-accodion-icon-style-arrowcircle )  > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap .kt-blocks-accordion-icon-trigger:before{background:#444444;}.kt-accordion-id19922_23959d-79:not( .kt-accodion-icon-style-basic ):not( .kt-accodion-icon-style-xclose ):not( .kt-accodion-icon-style-arrow ) 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transparent;border-left:1px solid transparent;}}@media all and (max-width: 1024px){.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}}@media all and (max-width: 1024px){.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_23959d-79 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}}@media all and (max-width: 1024px){.kt-accordion-id19922_23959d-79 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}}@media all and (max-width: 767px){.kt-accordion-id19922_23959d-79 .kt-accordion-inner-wrap{display:block;}.kt-accordion-id19922_23959d-79 .kt-accordion-inner-wrap .kt-accordion-pane:not(:first-child){margin-top:10px;}.kt-accordion-id19922_23959d-79 .kt-accordion-panel-inner{border-top:0px solid transparent;border-right:1px solid transparent;border-bottom:1px solid transparent;border-left:1px solid transparent;}.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header{border-top:1px solid #eeeeee;border-right:1px solid #eeeeee;border-bottom:1px solid #eeeeee;border-left:2px solid #eeeeee;}.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header:hover, \n\t\t\t\tbody:not(.hide-focus-outline) .kt-accordion-id19922_23959d-79 .kt-blocks-accordion-header:focus-visible{border-top-color:#878787;border-top-style:solid;border-right-color:#878787;border-right-style:solid;border-bottom-color:#878787;border-bottom-style:solid;border-left-color:#878787;border-left-style:solid;}.kt-accordion-id19922_23959d-79 .kt-accordion-header-wrap .kt-blocks-accordion-header:focus-visible,\n\t\t\t\t.kt-accordion-id19922_23959d-79 > .kt-accordion-inner-wrap > .wp-block-kadence-pane > .kt-accordion-header-wrap > .kt-blocks-accordion-header.kt-accordion-panel-active{border-top-color:#eeeeee;border-top-style:solid;border-right-color:#eeeeee;border-right-style:solid;border-bottom-color:#eeeeee;border-bottom-style:solid;border-left-color:#0e9cd1;border-left-style:solid;}}<\/style>\n<div class=\"wp-block-kadence-accordion alignnone\"><div class=\"kt-accordion-wrap kt-accordion-id19922_23959d-79 kt-accordion-has-2-panes kt-active-pane-0 kt-accordion-block kt-pane-header-alignment-left kt-accodion-icon-style-arrow kt-accodion-icon-side-right\" style=\"max-width:none\"><div class=\"kt-accordion-inner-wrap\" data-allow-multiple-open=\"false\" data-start-open=\"0\">\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-1 kt-pane19922_a7a7d6-fd\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong>Do I need to change my instrument or assay?<\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p>No. The comparison is designed to use your existing Bio-Rad real-time PCR system, assays, and samples. Follow the recommended starting conditions in the evaluation guide and compare your current supermix with an Applied Biosystems Master Mix.<\/p>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-kadence-pane kt-accordion-pane kt-accordion-pane-2 kt-pane19922_3d1aaa-0f\"><h2 class=\"kt-accordion-header-wrap\"><button class=\"kt-blocks-accordion-header kt-acccordion-button-label-show\" type=\"button\"><span class=\"kt-blocks-accordion-title-wrap\"><span class=\"kt-blocks-accordion-title\"><strong>How do I request an evaluation sample?<\/strong><\/span><\/span><span class=\"kt-blocks-accordion-icon-trigger\"><\/span><\/button><\/h2><div class=\"kt-accordion-panel kt-accordion-panel-hidden\"><div class=\"kt-accordion-panel-inner\">\n<p>Click <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\">Challenge Your Supermix<\/a> or <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\">Request a Sample<\/a> to request an Applied Biosystems Master Mix sample. Download the evaluation guide that matches your workflow, then perform a side-by-side comparison using your own assays, samples, and real-time PCR system. Technical support is available if you need assistance during the evaluation.<\/p>\n<\/div><\/div><\/div>\n<\/div><\/div><\/div>\n\n\n\n<div class=\"wp-block-tf-blocks-internal-links\">\n<h3 class=\"wp-block-heading\" id=\"h-related-resources\">Related Resources<\/h3>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/qpcr-master-mix-sample.html\" target=\"_blank\" rel=\"noreferrer noopener\">Applied Biosystems Real-Time PCR Master Mix Sample Request Program<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/documents.thermofisher.com\/TFS-Assets\/GSD\/Application-Notes\/evaluate-qpcr-performance-app-note.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Real-time PCR Reagent Comparison Guide<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/reagents-kits\/resources.html#evaluate\" target=\"_blank\" rel=\"noreferrer noopener\">Master Mix Evaluation Resources<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/documents.thermofisher.com\/TFS-Assets\/GSD\/Reference-Materials\/taqman-fast-advanced-master-mix-evaluation-guide.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">TaqMan Fast Advanced Master Mix Evaluation Guide<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/documents.thermofisher.com\/TFS-Assets\/GSD\/Reference-Materials\/powerup-sybr-green-master-mix-evaluation-guide.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">PowerUp SYBR Green Master Mix Evaluation Guide<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/4444556\" target=\"_blank\" rel=\"noreferrer noopener\">TaqMan Fast Advanced Master Mix Product Page<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A25741\" target=\"_blank\" rel=\"noreferrer noopener\">PowerUp SYBR &nbsp;Green Master Mix Product Page<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A46012\" target=\"_blank\" rel=\"noreferrer noopener\">PowerTrack SYBR Green Master Mix Product Page<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/reagents-kits.html\" target=\"_blank\" rel=\"noreferrer noopener\">Real-Time PCR Reagents and Kits<\/a><br><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/reagents-kits\/resources.html\" target=\"_blank\" rel=\"noreferrer noopener\">Technical Support<\/a><\/li>\n<\/ul>\n<\/div>\n\n\n\n<p class=\"has-small-font-size\">For Research Use Only. Not for use in diagnostic procedures. \u00a9 2026 Thermo Fisher Scientific Inc. All rights reserved. Bio-Rad, CFX Opus, SsoAdvanced, iTaq, and iQ are trademarks of Bio-Rad Laboratories, Inc. TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license. All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.<\/p>\n\n\n\n<p class=\"has-small-font-size\"><strong>Terms and conditions:<\/strong> No purchase necessary. This promotion is available to life science professionals 21 years or older. Approx. retail value of sample is $125\u2013$525 USD depending on the sample selected. Promotion runs until sample supplies are depleted. The sample volume is 1 mL. Limit one free sample per customer. By accepting the sample, you warrant that you are not prohibited by employment, contract, or law from accepting a sample from Life Technologies and that your employer has full knowledge of your actions and has consented to you receiving a free sample from Life Technologies. Cannot be combined with other discounts or promotions. Offer void where prohibited, licensed, or restricted by federal, state, provincial, or local laws or regulation or agency\/institutional policy. Other restrictions may apply.<\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>The chemistry of your qPCR supermix can influence analytical sensitivity, specificity, precision, and overall assay performance. Even when you keep the same instrument, assays, and samples, different master mixes can perform differently. That&#8217;s why we challenge you to put your supermix to the test. Compare it side by side with an Applied Biosystems Master Mix<\/p>\n","protected":false},"author":1793,"featured_media":19937,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[172,108,101],"tags":[2706,2707,2705,2704,2700,2701,2702,2703,2318],"division":[2575],"class_list":{"0":"post-19922","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-general","8":"category-lab-tips-and-tricks","9":"category-product-news","10":"tag-assay-specificity","11":"tag-melt-curve-analysis","12":"tag-pcr-efficiency","13":"tag-probe-based-qpcr","14":"tag-qpcr-master-mix","15":"tag-qpcr-master-mix-comparison","16":"tag-qpcr-performance","17":"tag-qpcr-reagents","18":"tag-sybr-green","19":"division-gsd","20":"entry"},"_selected_authors":[1552],"_selected_reviewers":[],"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>qPCR Master Mix Comparison: Test Your Supermix<\/title>\n<meta name=\"description\" content=\"Compare qPCR master mix performance for sensitivity, precision, specificity, and PCR efficiency using your existing assays, samples, and instrument.\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/qpcr-master-mix-comparison\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Think your supermix is good enough? 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The benefits are clear: reduced sample consumption, lower costs, faster workflows, and more data from every experiment. However, designing successful multiplex assays isn't always straightforward. Traditionally, identifying problematic assay combinations required extensive wet-lab\u2026","rel":"","context":"In &quot;Lab Tips and Tricks&quot;","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"For Research Use Only. Not for use in diagnostic procedures. \u00a9 2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19685,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-qpcr-primer-optimization\/","url_meta":{"origin":19922,"position":3},"title":"Multiplex qPCR primer optimization: How to balance amplification across targets","author":"Ashleigh Barlow","date":"May 1, 2026","format":false,"excerpt":"What happens when one target amplifies more efficiently than another in a multiplex qPCR reaction? In many experiments, targets are not present at equal abundance. Some amplify earlier, generate stronger signals, and begin to dominate the reaction. Others may appear delayed, or not at all. These effects are not always\u2026","rel":"","context":"In &quot;Lab Tips and Tricks&quot;","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"DNA strands with multicolored signals representing simultaneous detection of multiple targets in multiplex qPCR.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Blog_qPCR_Reagent_multiplex_qPCR_primerlimitations-1-Multiplex-qPCR_hero.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Blog_qPCR_Reagent_multiplex_qPCR_primerlimitations-1-Multiplex-qPCR_hero.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Blog_qPCR_Reagent_multiplex_qPCR_primerlimitations-1-Multiplex-qPCR_hero.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Blog_qPCR_Reagent_multiplex_qPCR_primerlimitations-1-Multiplex-qPCR_hero.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Blog_qPCR_Reagent_multiplex_qPCR_primerlimitations-1-Multiplex-qPCR_hero.jpeg?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19748,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/andes-virus-rt-qpcr-detection-workflow\/","url_meta":{"origin":19922,"position":4},"title":"Responding to Hantavirus: Advancing Rapid Molecular Detection","author":"Behind The Bench Staff","date":"May 14, 2026","format":false,"excerpt":"Article Summary Laboratories responding to the emerging 2026 Andes hantavirus incident need rapid, scalable molecular workflows that can integrate into existing RT-qPCR infrastructure. This workflow is best suited for public health laboratories, surveillance programs, academic research labs, and high-throughput testing facilities seeking rapid research-use-only (RUO) molecular detection capabilities. By combining\u2026","rel":"","context":"In &quot;Infectious Diseases&quot;","block_context":{"text":"Infectious Diseases","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/infectious-diseases\/"},"img":{"alt_text":"Illustration of hantavirus transmission showing rodent-derived viral particles entering the human respiratory system.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/05\/Hantavirus-3-1_Geoffrey-Cassell.png?resize=1050%2C600&ssl=1 3x"},"classes":[]},{"id":19271,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-qpcr-basics-guide\/","url_meta":{"origin":19922,"position":5},"title":"Multiplex vs. Singleplex qPCR: What do I need to know?","author":"Ashleigh Barlow","date":"December 3, 2025","format":false,"excerpt":"Real-time PCR (qPCR) has transformed how scientists detect and quantify nucleic acid targets, offering analytical sensitivity, speed, and scalability. Many real-time PCR reactions measure a single target per well \u2014 a setup called singleplex qPCR. However, the effectiveness of real-time PCR can be increased by an approach called multiplex qPCR.\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=700%2C400&ssl=1 2x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/Untitled-August-18-2026-at-08.39.51-5.jpeg","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/19922","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/1793"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=19922"}],"version-history":[{"count":4,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/19922\/revisions"}],"predecessor-version":[{"id":19941,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/19922\/revisions\/19941"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media\/19937"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=19922"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=19922"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=19922"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=19922"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}