{"id":7011,"date":"2015-06-25T14:54:31","date_gmt":"2015-06-25T14:54:31","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=7011"},"modified":"2020-09-26T00:10:43","modified_gmt":"2020-09-26T00:10:43","slug":"sanger-sequencing-by-ce-6-resources","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-by-ce-6-resources\/","title":{"rendered":"Sanger Sequencing by CE 6: Resources"},"content":{"rendered":"<p>We at Behind the Bench hope you have enjoyed this series on Sanger Sequencing by Capillary Electrophoresis, and we conclude with this final post to point out different online resources to access.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-7017\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/12\/sangeredu2.jpg\" alt=\"DNA strand\" width=\"250\" height=\"200\" \/><\/p>\n<p>There are three <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/fragment-analysis-guide-registration-form.html?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">guides available to download<\/a>. The first, the Fragment Analysis Guide, is a 218-page electronic PDF comprehensive in its scope. This guide covers topics from experimental design (including different dye and size standards and primer design considerations), to optimization of PCR, to optimization of capillary electrophoresis for fragment analysis (at instrument-specific detail). It also includes sections on STR, SNP genotyping, different fingerprinting methods, and software analysis tools, in addition to an extensive troubleshooting section.<\/p>\n<p>The second guide, DNA Sequencing by Capillary Electrophoresis: Applied Biosystems Chemistry Guide, is a 310-page electronic PDF covering all of the details of the process, from template preparation and cycle sequencing to purification of products and capillary electrophoresis, and then data analysis. There is also a troubleshooting section.<\/p>\n<p>The third guide, the Capillary Electrophoresis Catalog, is a comprehensive product catalog that lists all of the Sanger sequencing-related products in one place.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-7018 alignright\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2015\/12\/sangeredu.jpg\" alt=\"sangeredu\" width=\"230\" height=\"195\" \/><\/p>\n<p>Thermo Fisher Scientific offers on-site training for Sanger Sequencing, as well as trainings regularly held at one of our regional training centers. Here is the webpage with more <a href=\"https:\/\/learn.thermofisher.com\/dnasequencing?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">information<\/a>, or you can email <a href=\"mailto:customertraining@thermofisher.com\" target=\"_blank\" rel=\"noopener noreferrer\">customertraining@thermofisher.com<\/a>.<\/p>\n<p>If you are looking for software support, here\u2019s our <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/technical-resources\/technical-reference-library\/capillary-electrophoresis-software-support-center.html?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Capillary Electrophoresis Software Support Center<\/a> with links to Getting Started and Troubleshooting help for both fragment analysis and Sanger sequencing applications. In addition, each respective section has links to PDF files of the datasheets for the respective software application.<\/p>\n<p>Over the next few months keep an eye out for a new technical question video series called \u2018Seq-It-Out\u2019, and of course feel free to contact our excellent <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/technical-resources\/contact-us.html?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Technical Support group<\/a> directly with any questions.<\/p>\n<p>Check out the whole Series:<\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-by-ce-1-foundations\/?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Sanger Sequencing by CE 1: Foundations<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-using-ce-2-fragment-analysis\/?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Sanger Sequencing using CE 2: Fragment Analysis<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/using-ce-3-designing-a-27-plex-pcr-for-fragment-analysis\/?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Fragment Analysis using CE 3: Designing a 27-plex PCR<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-by-ce-4-bioinformatics\/?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Sanger Sequencing by CE 4: Bioinformatics<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-by-ce-5-sanger-sequencing-applications\/?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Sanger Sequencing by CE 5: Sanger Sequencing Applications<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-by-ce-6-resources\/?cid=social_btb_abseq\" target=\"_blank\" rel=\"noopener noreferrer\">Sanger Sequencing by CE 6: Resources<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We at Behind the Bench hope you have enjoyed this series on Sanger Sequencing by Capillary Electrophoresis, and we conclude with this final post to point out different online resources to access. There are three guides available to download. The first, the Fragment Analysis Guide, is a 218-page electronic PDF comprehensive in its scope. This<\/p>\n","protected":false},"author":120,"featured_media":7018,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[108],"tags":[17,36,99,25,74],"division":[],"class_list":{"0":"post-7011","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-lab-tips-and-tricks","8":"tag-applied-biosystems","9":"tag-capillary-electrophoresis","10":"tag-fragment-analysis","11":"tag-sanger-sequencing","12":"tag-sequencing","13":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Sanger Sequencing by CE 6: Resources - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/sanger-sequencing-by-ce-6-resources\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Sanger Sequencing by CE 6: Resources\" \/>\n<meta property=\"og:description\" content=\"We at Behind the Bench hope you have enjoyed this series on Sanger Sequencing by Capillary Electrophoresis, and we conclude with this final post to point out different online resources to access. 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Today\u2019s labs are not only looking for accuracy\u2014they need speed, flexibility, and reliable\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"DNA sequencing sample tube with DNA helix and sequencing chromatogram representing Sanger sequencing workflow.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/ChatGPT-Image-Aug-5-2026-09_44_12-AM.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19509,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/cfdna-fragmentomics-analysis-qpcr-cancer-monitoring\/","url_meta":{"origin":7011,"position":1},"title":"qPCR-based fragmentomics for monitoring cancer disease progression","author":"Behind The Bench Staff","date":"March 5, 2026","format":false,"excerpt":"As fragmentomics gains momentum in cancer liquid biopsy research, attention is increasingly turning to how these structural cfDNA signals can be measured in a way that is both sensitive and practical. Established qPCR platforms offer an alternative to sequencing-based approaches for capturing fragment size information with high precision. Using a\u2026","rel":"","context":"In &quot;Bioinformatics&quot;","block_context":{"text":"Bioinformatics","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/bioinformatics\/"},"img":{"alt_text":"Scientist pipetting into a 96 well plate during qPCR workflow for cell free DNA fragmentomics analysis","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Picture1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Picture1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Picture1.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":19810,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/integration-site-analysis-gene-therapy-safety\/","url_meta":{"origin":7011,"position":2},"title":"Genotoxicity, vectors, and the power of integration site analysis","author":"Ashleigh Barlow","date":"June 23, 2026","format":false,"excerpt":"Article Summary In this episode of Absolute Gene-ius, Michael Stump, PhD, President of ProtaGene, and Matteo Franco, Lead Scientist at ProtaGene, discuss how integration site analysis is helping researchers evaluate genotoxicity risks in cell and gene therapy workflows. Their conversation explores how viral vectors integrate into the genome, why integration\u2026","rel":"","context":"In &quot;Gene Editing&quot;","block_context":{"text":"Gene Editing","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/gene-editing\/"},"img":{"alt_text":"Matteo Franco, Lead Scientist at ProtaGene, discussing integration site analysis and genotoxicity assessment","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19883,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/building-a-better-sample-qc-workflow-from-extraction-to-sequencing\/","url_meta":{"origin":7011,"position":3},"title":"Building a Better Sample QC Workflow: From Extraction to Sequencing","author":"Marlene Gasdia-Cochrane","date":"August 25, 2026","format":false,"excerpt":"Article Summary Successful genomics experiments don't begin with sequencing\u2014they begin with sample quality. Every downstream application, from PCR to next-generation sequencing (NGS), depends on starting with nucleic acids that are accurately quantified and free from contaminants that could compromise results. Establishing a consistent quality control workflow helps laboratories improve reproducibility\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"A pipette drops a liquid into a petri dish with a dna sequence background, science concept. 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With continuous advancements, qPCR remains a critical tool for researchers worldwide. 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