{"id":7026,"date":"2015-03-30T16:35:46","date_gmt":"2015-03-30T16:35:46","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=7026"},"modified":"2020-05-28T19:14:26","modified_gmt":"2020-05-28T19:14:26","slug":"go-digital-pcr-pt-2-quantstudio-3d","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/go-digital-pcr-pt-2-quantstudio-3d\/","title":{"rendered":"Go Digital PCR: Pt 2 \u2013 QuantStudio\u2122 3D"},"content":{"rendered":"

How to deliver absolutely attainable digital PCR, simply and affordably<\/span><\/p>\n

This is the second in a six part series to introduce you to digital PCR (dPCR). In this edition, we\u2019ll take a look at how QuantStudio\u2122 3D dPCR from Thermo Fisher Scientific\u00a0offers simplicity, affordability, and most importantly, absolute quantification attainability.<\/p>\n

QuantStudio\u2122 3D Digital PCR System helps expand the use of dPCR<\/strong><\/p>\n

Cumbersome workflows and costly implementation have long been barriers to dPCR adoption in the lab. Thermo Fisher Scientific has made progress in bringing load-and-go simplicity to dPCR and significantly lowering the price of entry with the introduction of its QuantStudio\u2122 3D Digital PCR System<\/a>.<\/p>\n

dPCR expands the application boundaries of traditional real-time PCR by enabling absolute quantification without the use of a standard curve. The ability to move beyond measuring Ct<\/sub> values to detecting individual DNA molecules provides researchers with additional sensitivity and precision for a variety of experiments. QuantStudio\u2122 3D research experiments include, but not limited to: Rare cancer mutation quantification<\/a>, copy number variation (CNV) analysis<\/a>, pathogen detection and load determination<\/a>, absolute quantification of standards<\/a>,<\/p>\n

library quantification for next-generation sequencing (NGS)<\/a>, characterization of low-fold changes in mRNA and miRNA expression<\/a>, and GMO detection and contamination assessment<\/a>.<\/p>\n

Simplicity, affordability, and absolute quantification at a fraction of the size, cost, and number of hands-on steps compared to other digital PCR and real-time qPCR methods sets the stage for an increasing number of labs to optimize their nucleic acid detection and quantification capabilities.<\/p>\n

A simpler-is-better workflow improves process efficiencies<\/strong><\/p>\n

Seeing is believing. To view a brief video demonstration of how easy it is to load and read chips on the QuantStudio\u2122 3D, click here<\/a>.<\/p>\n

QuantStudio\u2122 3D is designed to minimize the level of expertise needed to perform dPCR. Users simply load the reaction mix onto the uniquely tagged chip, amplify on a dual flat block thermal cycler, and read the target concentration (reading takes less than 30 seconds). Up to 24 chips can be loaded at one time.<\/p>\n

\"3d<\/p>\n

When performing dPCR, a nucleic acid mixture is partitioned into many reaction wells, with some wells receiving a target molecule and some not. Reactions are then subjected to standard PCR and then scanned to identify wells that have not received target molecules.<\/p>\n

\"dpcr2\"<\/p>\n

A standard statistical correction, or Poisson model, accounts for wells that may have received more than a single target molecule, and a final concentration value is determined. Given that dPCR doesn\u2019t rely on Ct<\/sub> values to quantify copy number, comparison to a known standard is not required for absolute quantification.<\/p>\n

The QuantStudio\u2122 3D leverages high-density nanofluidic chip technology to partition a sample into as many as 20,000 independent reaction wells. Comprised of consistently sized wells etched in a solid silicon substrate, the chip enables straightforward and robust sample partitioning that allows thousands of data points to be analyzed per run.<\/p>\n

\"dpcr3\"<\/p>\n

Samples and amplification products remain completely contained throughout the streamlined process, and an easily interpretable answer \u2013 in target copies\/\u03bcL \u2013 is produced.<\/p>\n

The QuantStudio\u2122 3D is capable of detecting both TaqMan\u00ae <\/sup>and SYBR\u2122 chemistries. TaqMan\u00ae<\/sup> chemistry minimizes false positives in quantitative PCR experiments and minimizes the need for melt curve analysis. Over 8 million predesigned TaqMan\u00ae<\/sup> Assays are available for dPCR applications using the QuantStudio\u2122 3D.<\/p>\n

Perform absolute quantification with confidence <\/strong><\/p>\n

The QuantStudio\u2122 3D makes a data quality assessment for each calculation that determines absolute number copies\/\u03bcL. Data considered marginal or failing are flagged for further review in secondary analysis using Thermo Fisher AnalysisSuite\u2122<\/sup> Cloud Software<\/a>. The software supports secondary analysis from any computer connected to the Internet through a personalized, cloud-based ThermoFisher Scientific user account. Data transfer is seamless and fully automated, but can also be transferred over an internal network or via USB flash drive.<\/p>\n

Analysis Suite\u2122<\/sup> software is also available for further data QC and multichip analysis for specific applications. Chip data are uploaded to a secure environment and analyzed using different application modules such as absolute quantification and relative quantification, with visualizations allowing for chip inspection, clustering, and CNV calculation.<\/p>\n

Affordable enough to enable dPCR in any lab<\/strong><\/p>\n

Compared to its closest dPCR competitor, it would take at least 10 years of average use, i.e. 1000 sample runs per year, to equal the savings achieved with the QuantStudio\u2122 3D. At this low price point it opens the door for more labs to upgrade and streamline their nucleic acid detection and absolute quantification capabilities. Because we offer more than 8 million TaqMan\u00ae<\/sup> assays available for dPCR, labs can further reduce time and procurement costs.<\/p>\n

dPCR Augmented Reality App puts cutting-edge technology at your fingertips<\/strong><\/p>\n

Take a virtual tour<\/a> of the QuantStudio\u2122 3D to experience its innovative features and sealed, chip-based workflow.<\/p>\n

Once you\u2019ve completed the tour, you can download the augmented reality app to your iOS\u2122<\/sup><\/a> or Android\u2122<\/sup> <\/a>
\ndevice, print the augmented reality (AR) target, and practice using it in your spare time to hone your dPCR skills.<\/p>\n

The app provides a good overview of and firsthand interaction with QuantStudio\u2122 3D absolute quantification capabilities, including:<\/p>\n

\u2022\u00a0 Augmented reality learning module to guide you through the key areas of dPCR, including absolute quantification and rare target detection<\/p>\n

\u2022\u00a0 17-minute video, with easy-to-understand illustrations<\/p>\n

\u2022\u00a0 Interactive workflow guide showcasing chip-based technology<\/p>\n

\u2022\u00a0 Snap and share photos of the virtual QuantStudio 3D right from your lab<\/p>\n

\u2022\u00a0 Connection to online learning resources from Thermo Fisher Scientific, including the Applied Biosystems\u2122 User Community<\/p>\n

Conclusion<\/strong><\/p>\n

That\u2019s a lot to absorb in such a short time, so I\u2019ll wrap up with the highlights and leave you to explore more about how to move your lab beyond real-time qPCR to dPCR with QuantStudio\u2122 3D.<\/p>\n

From the top, simpler is better. QuantStudio\u2122 3D uses a chip-based workflow with minimal hands-on steps and sample manipulation to help increase precision and sensitivity.<\/p>\n

Next, it puts absolute quantification at your fingertips with the help of precision controlled nanofluidic technology that can deliver up to 20,000 individual partitions in copies\/\u03bcL per chip, without the use of a standard curve.<\/p>\n

And, finally, QuantStudio\u2122 3D is available to labs at a cost that\u2019s less than half the price of competing platforms.<\/p>\n

Learn More about QuantStudio\u2122 3D Digital PCR<\/strong><\/p>\n

Learn more about how the QuantStudio\u2122 3D Digital PCR System<\/a> is delivering simplicity, affordability, and absolute quantification attainability to a growing number of labs everywhere. Plus, find a full complement of dPCR instrument support needs<\/a> in a single online source, including relevant technical information, tips and tricks when starting an experiment, and fixes for common instrument and software errors.<\/p>\n

Beginner and intermediate level dPCR Webinars<\/a> are also available on demand to provide a deeper level of understanding of the full range dPCR benefits and a more in-depth look at the QuantStudio\u2122 3D.<\/p>\n

Read all of the Let’s Go Digital PCR Series:<\/p>\n

Go Digital PCR: Pt 1 \u2013 Next-Gen Quantification<\/a><\/p>\n

Go Digital PCR: Pt 2 \u2013 QuantStudio\u2122 3D<\/a><\/p>\n

Go Digital PCR: Pt 3 – Copy Number Variation<\/a><\/p>\n

Go Digital PCR: Pt 4 \u2013 NGS Libraries<\/a><\/p>\n

Go Digital PCR: Pt 5 \u2013 Rare Mutations<\/a><\/p>\n

Go Digital PCR: Pt 6 – Emerging Analyses<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

How to deliver absolutely attainable digital PCR, simply and affordably This is the second in a six part series to introduce you to digital PCR (dPCR). In this edition, we\u2019ll take a look at how QuantStudio\u2122 3D dPCR from Thermo Fisher Scientific\u00a0offers simplicity, affordability, and most importantly, absolute quantification attainability. QuantStudio\u2122 3D Digital PCR System<\/p>\n","protected":false},"author":120,"featured_media":7030,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[108,101],"tags":[17,57,43],"division":[],"class_list":{"0":"post-7026","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-lab-tips-and-tricks","8":"category-product-news","9":"tag-applied-biosystems","10":"tag-digital-pcr","11":"tag-pcr","12":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nGo Digital PCR: Pt 2 \u2013 QuantStudio\u2122 3D - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/go-digital-pcr-pt-2-quantstudio-3d\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Go Digital PCR: Pt 2 \u2013 QuantStudio\u2122 3D\" \/>\n<meta property=\"og:description\" content=\"How to deliver absolutely attainable digital PCR, simply and affordably This is the second in a six part series to introduce you to digital PCR (dPCR). 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